Complement C4d Antibody (C4D204)

Immunohistochemistry-Paraffin: Complement C4d Antibody (C4D204) [NBP2-34234] - Formalin-fixed, paraffin-embedded human kidney transplant tissue (20X) stained with Complement 4d MAb (C4D204).

Immunohistochemistry-Paraffin: Complement C4d Antibody (C4D204) [NBP2-34234] - Formalin-fixed, paraffin-embedded human kidney transplant tissue (10X) stained with Complement 4d MAb (C4D204).

Immunohistochemistry-Paraffin: Complement C4d Antibody (C4D204) [NBP2-34234] - Formalin-fixed, paraffin-embedded human kidney transplant tissue (40X) stained with Complement 4d Ab (C4D204).

Immunohistochemistry-Paraffin: Mouse Monoclonal Complement C4d Antibody (C4D204) [NBP2-34234] - Analysis of C4d on human lymph node tissue. Follicular dendritic cell are positive for C4d. pAb: 1:200 anti-mouse DAB epitope retrieval in basic buffer. Image from a verified customer review.

• Reactivity: Hu
• Applications: ELISA, ICC/IF, IHC
• Clone: C4D204
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Complement C4d Antibody (C4D204) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34581)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Recombinant human Complement Complement C4d protein
• Localization: Intracytoplasmic vacuoles of endothelial cells and Secreted
• Marker: Acute Humoral Rejection Marker
• Specificity: This monoclonal antibody is specific to Complement 4d (C4d) and reacts with the secreted as well as cell-bound C4d.C4d is a degradation product of the activated complement factor C4b. Complement 4b is typically activated by binding of Abs to specific target molecules. Following activation and degradation of the C4 molecule, thio-ester groups are exposed, which allow transient, covalent binding of the degradation product Complement 4d to endothelial cell surfaces and extracellular matrix components of vascular basement membranes near the sites of C4 activation. The presence of C4d in peritubular capillaries is a key indicator for acute humoral (i.e. antibody-mediated) rejection of kidney, heart, pancreas and lung allografts. As an established marker of antibody-mediated acute renal allograft rejection and its proclivity for endothelium, this component can be detected in peritubular capillaries in chronic renal allograft rejection as well as hyperacute rejection, acute vascular rejection, acute cellular rejection, and borderline rejection. It has been shown to be a significant predictor of transplant kidney graft survival. Anti-C4d, combined with anti-C3d, can be utilized as a tool for diagnosis of allograft rejection that may warrant a prompt and aggressive anti-rejection treatment.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: C4A
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1-2 ug/ml
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200-1:400
• Application Notes: ELISA: For coating use Ab at 1-2ug/ml, order Ab without BSA.
  Immunohistochemistry (Formalin-fixed): 1:200-1:400 for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 7.5-8.5, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 192 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Complement C4d Antibody (C4D204)

• C4
• C4A
• C4A2
• C4A3
• C4A4
• C4A6
• C4S
• CO4
• complement C4A
• CPAMD2
• RG

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Complement C4d Antibody (NBP2-34234)(1)

Publication using NBP2-34234

• Katschke KJ, Xi H, Cox C et al. Classical and alternative complement activation on photoreceptor outer segments drives monocyte-dependent retinal atrophy Sci Rep 2018-05-09 [PMID: 29743491] (ICC/IF, Human)

Review for Complement C4d Antibody (NBP2-34234) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IHC-P Human 02/05/2025

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Lymph node
• Species: Human
• Lot: 720-1P240823

Comments

• Comments: pAb: 1:200
  anti-mouse
  DAB
  epitope retrieval in basic buffer

Bioinformatics

• Gene Symbol: C4A