Lamin B Receptor Antibody

Images

 
Western Blot: Lamin B Receptor Antibody [H00003930-B01P] - Analysis of LBR expression in transfected 293T cell line by LBR polyclonal antibody. Lane 1: LBR transfected lysate(67.65 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: Lamin B Receptor Antibody [H00003930-B01P] - Analysis of purified antibody to LBR on HeLa cell. (antibody concentration 10 ug/ml)
Immunohistochemistry-Paraffin: Lamin B Receptor Antibody [H00003930-B01P] - Analysis of purified antibody to LBR on formalin-fixed paraffin-embedded human small Intestine. (antibody concentration 3 ug/ml)
Western Blot: Lamin B Receptor Antibody [H00003930-B01P] - Expression in Jurkat.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Lamin B Receptor Antibody Summary

Immunogen
LBR (NP_002287.2, 1 a.a. - 615 a.a.) full-length human protein. MPSRKFADGEVVRGRWPGSSLYYEVEILSHDSTSQLYTVKYKDGTELELKENDIKPLTSFRQRKGGSTSSSPSRRRGSRSRSRSRSPGRPPKSARRSASASHQADIKEARREVEVKLTPLILKPFGNSISRYNGEPEHIERNDAPHKNTQEKFSLSQESSYIATQYSLRPRREEVKLKEIDSKEEKYVAKELAVRTFEVTPIRAKDLEFGGVPGVFLIMFGLPVFLFLLLLMCKQKDPSLLNFPPPLPALYELWETRVFGVYLLWFLIQVLFYLLPIGKVVEGTPLIDGRRLKYRLNGFYAFILTSAVIGTSLFQGVEFHYVYSHFLQFALAATVFCVVLSVYLYMRSLKAPRNDLSPASSGNAVYDFFIGRELNPRIGTFDLKYFCELRPGLIGWVVINLVMLLAEMKIQDRAVPSLAMILVNSFQLLYVVDALWNEEALLTTMDIIHDGFGFMLAFGDLVWVPFIYSFQAFYLVSHPNEVSWPMASLIIVLKLCGYVIFRGANSQKNAFRKNPSDPKLAHLKTIHTSTGKNLLVSGWWGFVRHPNYLGDLIMALAWSLPCGFNHILPYFYIIYFTMLLVHREARDEYHCKKKYGVAWEKYCQRVPYRIFPYIY
Marker
Nuclear Inner Membrane Marke
Specificity
LBR,
Isotype
IgG
Clonality
Polyclonal
Host
Mouse
Gene
LBR
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Control
Lamin B Receptor Knockout 293T Cell Lysate
Reviewed Applications
Read 1 Review rated 3
using
H00003930-B01P in the following applications:

Reactivity Notes

Mouse samples were tested by customer review, so the antibody may cross react with the mouse protein.Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein A purified

Notes

Quality control test: Antibody reactive against mammalian transfected lysate.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Lamin B Receptor Antibody

  • DHCR14B
  • FLJ43126
  • Integral nuclear envelope inner membrane protein
  • lamin B receptor
  • lamin-B receptor
  • LMN2R
  • MGC9041
  • PHA

Background

The protein encoded by this gene belongs to the ERG4/ERG24 family. It localized in the nuclear envelope inner membrane and anchors the lamina and the heterochromatin to the membrane. It may mediate interaction between chromatin and lamin B. Mutations of this gene has been associated with autosomal recessive HEM/Greenberg skeletal dysplasia. Alternative splicing occurs at this locus and two transcript variants encoding the same protein have been identified. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Lamin B Receptor Antibody (H00003930-B01P) (0)

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Review for Lamin B Receptor Antibody (H00003930-B01P) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using H00003930-B01P:
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IF
(1)
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(1)
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Images Ratings Applications Species Date Details
Immunofluorescence Lamin B Receptor H00003930-B01P
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reviewed by:
Anonymous
IF Mouse 09/27/2013
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Summary

ApplicationImmunofluorescence
Sample Testedmouse skin
SpeciesMouse
Lot11132

Blocking

Blocking Details0.1% BSA and 20% goat serum for 30 min at room temperature; Mouse to mouse blocking

Primary Anitbody

Dilution Ratio1:50 in PBS, overnight incubation at 4 degrees

Secondary Antibody

Secondary DescriptionAlexa 555 Goat anti-mouse
Secondary Manufacturer Cat#Invitrogen, A21422
Secondary Concentration1:100

Details

Detection NotesConfocal microscopy
Fixation Details4% PFA fixed,Paraffin embedded and/or Cryosection;Antigen retrieval with a pressure cooker in 0.5M EDTA solution (pH8) for 2 min
Wash DescriptionPBS, 5 min three times

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Lamin B Receptor Antibody (H00003930-B01P) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Recent Reviews

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1
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Anonymous
09/27/2013
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol LBR
Entrez
Uniprot