Rat Complement C4 ELISA Kit (Colorimetric)

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Rat Complement C4 ELISA Kit (Colorimetric) - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids.
Standard Curve Range
3.13 - 200 ng/mL
Sensitivity
1.88 ng/mL

Order Details

Rat Complement C4 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat C4 in samples. No significant cross-reactivity or interference between Rat C4 and analogues was observed.
Standard Curve Range
3.13 - 200 ng/mL
Sensitivity
1.88 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.18%
Intra-Assay
CV% < 5.17%
Spike Recovery
86-109%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
C4A

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Certificate of Analysis
  3. Concentrated Biotinylated Detection Ab (100×)
  4. Concentrated HRP Conjugate (100×)
  5. Concentrated Wash Buffer (25×)
  6. HRP Conjugate Diluent
  7. Micro ELISA Plate(Dismountable)
  8. Plate Sealer
  9. Product Description
  10. Reference Standard & Sample Diluent
  11. Reference Standard
  12. Stop Solution
  13. Substrate Reagent

Alternate Names for Rat Complement C4 ELISA Kit (Colorimetric)

  • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2
  • C4
  • complement component 4A (Rodgers blood group)
  • RG

Background

C4 consists of three polypeptide chains (alpha, molecular weight 95 kDa, beta, molecular weight 75 kDa and gamma, molecular weight 33 kDa) and is usually present in serum at 200-600mg/L. It is a protein of the classical complement pathway. When activated by C1s, a peptide C4a is released and the generated C4b binds C2 which is in turn cleaved by C1s to produce the classical pathway C3 convertase, namely C4b2a. C4b also has opsonin activity and binds to macrophages and neutrophils via their CR1 receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol C4A