Rat Complement C4 ELISA Kit (Colorimetric)

ELISA: Rat Complement C4 ELISA Kit (Colorimetric) [NBP2-70042] - Samples were spiked with high concentrations of Rat Complement C4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat Complement C4 ELISA Kit (Colorimetric) [NBP2-70042] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 3.13 - 200 ng/mL
• Sensitivity: 1.88 ng/mL

Rat Complement C4 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Rat C4 in samples. No significant cross-reactivity or interference between Rat C4 and analogues was observed.
• Standard Curve Range: 3.13 - 200 ng/mL
• Sensitivity: 1.88 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.18%
• Intra-Assay: CV% < 5.17%
• Spike Recovery: 86-109%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: C4A

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat Complement C4 ELISA Kit (Colorimetric)

• C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2
• C4
• complement component 4A (Rodgers blood group)
• RG

Background

C4 consists of three polypeptide chains (alpha, molecular weight 95 kDa, beta, molecular weight 75 kDa and gamma, molecular weight 33 kDa) and is usually present in serum at 200-600mg/L. It is a protein of the classical complement pathway. When activated by C1s, a peptide C4a is released and the generated C4b binds C2 which is in turn cleaved by C1s to produce the classical pathway C3 convertase, namely C4b2a. C4b also has opsonin activity and binds to macrophages and neutrophils via their CR1 receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Complement C4 ELISA Kit (NBP2-70042). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: C4A