Ataxin 1 Antibody (2F5)

Images

 
Western Blot: Ataxin 1 Antibody (2F5) [NBP1-51689] - Analysis using ATXN1 mab against HEK293 (1) and ATXN1 higGFC transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: Ataxin 1 Antibody (2F5) [NBP1-51689] - Immunofluorescence analysis of NTERA-2 cells using ATXN1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been ...read more
Immunohistochemistry-Paraffin: Ataxin 1 Antibody (2F5) [NBP1-51689] - Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues (left) and lung cancer tissues (right) using ATXN1 mouse mAb with DAB ...read more
Flow Cytometry: Ataxin 1 Antibody (2F5) [NBP1-51689] - Flow cytometric analysis of Jurket cells using ATXN1 mouse mAb (green) and negative control (purple).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Clone
2F5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Ataxin 1 Antibody (2F5) Summary

Immunogen
Purified recombinant fragment of human ATXN1 expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ATXN1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1/:000
  • Western Blot 1:500 - 1:2000
Theoretical MW
87 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for Ataxin 1 Antibody (2F5)

  • ataxin 1
  • ataxin 1)
  • ataxin-1
  • ATX1spinocerebellar ataxia 1 (olivopontocerebellar ataxia 1, autosomal dominant
  • SCA1D6S504E
  • Spinocerebellar ataxia type 1 protein

Background

Defects in Ataxin-1 are the cause of spinocerebellar ataxia type 1 (SCA1), also known as olivopontocerebellar atrophy I (OPCA I or OPCA1). Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to cerebellum degeneration with variable involvement of the brainstem and spinal cord. SCA1 is caused by expansion of a CAG repeat in the coding region of the ataxin-1 gene. Longer expansions result in earlier onset and more severe clinical manifestations of the disease. Ataxin-1 binds RNA in vitro and may be involved in RNA metabolism. [Uniprot]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Ataxin 1 Antibody (NBP1-51689)

Discover related pathways, diseases and genes to Ataxin 1 Antibody (NBP1-51689). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on Ataxin 1.

ATXN2 Identified as New Genetic Risk Factor for Lou Gehrig's Disease (ALS)
Ataxin antibodies are used in the study of autosomal dominant cerebellar ataxia (ADCA) diseases. These neurodegenerative disorders are highly heterogeneous, characterized by progressive, irreversible, atrophy of the cerebellum and spinal cord.Ataxin...  Read full blog post.

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Bioinformatics

Gene Symbol ATXN1