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Western Blot: Complement Factor B Antibody [NBP1-89985] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: Complement Factor B Antibody [NBP1-89985] - Staining of human cell line ASC TERT1 shows localization to endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Complement Factor B Antibody [NBP1-89985] - Staining of human prostate shows strong cytoplasmic positivity in glandular cells.
Western Blot: Complement Factor B Antibody [NBP1-89985] - Analysis in human plasma.
Immunohistochemistry-Paraffin: Complement Factor B Antibody [NBP1-89985] - Staining of human placenta shows strong positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: Complement Factor B Antibody [NBP1-89985] - Staining of human testis shows moderate positivity of secretion surrounding Leydig cells.
Immunohistochemistry-Paraffin: Complement Factor B Antibody [NBP1-89985] - Staining of human kidney shows moderate positivity in secretion surrounding cells in tubules.
Simple Western: Complement Factor B Antibody [NBP1-89985] - Simple Western lane view shows a specific band for CFB in 0.2 mg/ml of Tonsil lysate. This experiment was performed under reducing conditions using the 12-230 ...read more
Simple Western: Complement Factor B Antibody [NBP1-89985] - Electropherogram image(s) of corresponding Simple Western lane view. Complement Factor B antibody was used at 1:20 dilution on Tonsil lysate(s).
This antibody was developed against Recombinant Protein corresponding to amino acids: KDNEQHVFKVKDMENLEDVFYQMIDESQSLSLCGMVWEHRKGTDYHKQPWQAKISVIRPSKGHESCMGAVVSEYFVLTAAHCFTVDDKEHSIKVSVGGEKRDLEIEVVLFHPNYNINGKKEAGIPEFYDYDVALIKLKNKLKYG
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CFB
Purity
Immunogen affinity purified
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For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. IHC-Fr reported in literature (PMID: 26928779). ICC/IF reported in literature (PMID: 30220554).
Rat reactivity reported in scientific literature (PMID:30220554) and (PMID: 26928779).
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified
Alternate Names for Complement Factor B Antibody
BF AHUS4
BF
B-factor, properdin
BFD
C3 proaccelerator
C3 proactivator
C3/C5 convertase
CFAB
CFB
Complement Factor B
EC 3.4.21
EC 3.4.21.47
FB
FBI12
GBG
GBGCFAB
Glycine-rich beta glycoprotein
glycine-rich beta-glycoprotein
H2-Bf
PBF2
Properdin factor B
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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