CSRP1 Antibody

Western blot shows lysates of ME-180 human cervical epithelial carcinoma cell line, HT-2 mouse T cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human/Mouse/Rat CSRP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5739) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human CSRP1, CSRP2, CSRP3 (5 ng/lane) were included. A specific band for CSRP1 was detected at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CSRP1 Antibody Summary

• Immunogen: E. coli-derived recombinant human CSRP1
  Met1-Glu193
  Accession # P21291
• Specificity: Detects human, mouse, and rat CSRP1 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CSRP1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CSRP1 Antibody

• CRP
• CRP1
• CSRP1
• CSRPCYRP
• CYRP
• cysteine and glycine-rich protein 1
• Cysteine-rich protein 1
• D1S181E
• DKFZp686M148
• LIM-domain protein

Background

CSRP1 (Cysteine-rich protein 1; also CRP1 and Cysteine and Glycine‑rich protein 1) is a 23 kDa cytoplasmic member of the CRP family of LIM domain‑containing proteins. It is expressed in both visceral and vascular smooth muscle, and interacts with zyxin and alpha ‑actinin, two proteins involved in actin assembly and crosslinking, during muscle cell differentiation. Human CSRP1 is 193 amino acids (aa) in length and contains two 50 aa LIM (for lin11/isl1/mec3) domains that contain two zinc‑finger motifs (aa 10‑61 and 119‑171), an NLS between aa 64‑69, and a Gly-rich C-terminus (aa 176‑187). There are two likely phosphorylation sites at Tyr127 and Ser192. Full‑length human CSRP1 shares 99% aa identity with mouse CSRP1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CSRP1
• Uniprot:
  • P21291()