Immunohistochemistry-Paraffin: CD35 Antibody (E11) [NBP2-29460] - Formalin-fixed, paraffin-embedded human tonsil stained with CD35 antibody at 1:200 using peroxidase-conjugate and DAB chromogen. Note membrane staining.
Intact human monocytes were used as immunogen to generate the CD35 antibody (Hogg et al, 1984).
Follicular Dendritic Cell Marker
Recognizes a protein of 210-220kDa, which is identified as the complement receptor 1 (CR1)/CD35. This MAb is specific for a site in CR1 that is distal from the C3b-binding site, so that it is unable to block CR1 activity. This MAb is highly specific to CR1 and shows no cross-reaction with CR2. The primary function of CR1 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein. Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. They are CD21+/CD35+/CD1a-. This MAb labels follicular dendritic cells and follicular dendritic cell sarcoma.
Protein G purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
Human Chromosome Location: 1q32.2 Mol. Weight of Antigen: 210-220kDa Use in Western Blot has been reported in literature. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
215 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using NBP2-29460 in the following applications:
The clone E11 antibody reacts with CD35, also known as complement receptor 1 (CR1), a 210-220 kDa protein (Hogg et al, 1985). CD35 is present on a subpopulation of T lymphocytes (~5%) which are relatively low in CD3 expression. CD35 is also expressed on CD20 positive B lymphocytes, CD14 positive monocytes, and on poly-morphonuclear leukocytes. The primary function of CD35 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein. In Immunohistochemical staining (IHC) the CD35 antibody strongly labels the follicular dendritic cells (FDCs) and follicular dendritic cell sarcoma. Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. The antibody is highly specific for CD35 and does not cross react with CD21 (CR2). Furthermore, this CD35 antibody does not block CD35 activity because it recognizes a CD35 epitope distal to the C3b binding site. The antibody has been widely cited in the literature. Examples include its use in antibody panels for immunophenotyping normal bone marrow stem cells by flow cytometry (Laranjeira et al, 2012) and characterizing infections in rheumatoid arthritis patients (Moduko et al, 2012). The CD35 antibody has also been used along with CD23 and CD21 antibodies for identifying FDCs in reactive and neoplastic lymphoid tissue by IHC (Bagdi et al, 2001). Furthermore, the CD35 antibody showed than tonsil-derived mesenchymal progenitor cells acquire an FDC-like phenotype following cytokine stimulation (Cho et al, 2012), and that oncolytic measles viral infection upregulates CD35 (Zhang et al, 2012). Additional publications using the CD35 antibody can be identified through a key word on scholar.google.com.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
Review this Product
Be the first to review our CD35 Antibody (E11) and receive a gift card or discount.
PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.