c-Myc Antibody (9E11)


Western Blot: c-Myc Antibody (9E11) [NB200-108] - Whole cell protein from PC3 (lane 1), U-2 OS (lane 2) and mouse testis (lane 3) was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% ...read more
Flow Cytometry: c-Myc Antibody (9E11) [NB200-108] - c-Myc antibody was tested at 1:400 in HL-60 cells using an Alexa Fluor 488 secondary (shown in purple). M1 is defined by unstained cells.

Product Details

Reactivity Hu, Mu, Ch, YeSpecies Glossary
Applications WB, ChIP, ELISA, Flow, IHC, IHC-Fr, IHC-P, IP
1.0 mg/ml

c-Myc Antibody (9E11) Summary

A synthetic peptide corresponding to amino acids 408-420 (AEEQKLISEEDL) of human c-Myc, conjugated to KLH. [UniProt# P01106]
IgG2a Kappa
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1.0 mg/ml
Protein A or G purified


  • Western Blot 1:500-1:1000
  • Chromatin Immunoprecipitation 2 ug / 500 ug extract
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:200-1:400
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 2ug/mg lysate
Application Notes
This c-Myc antibody (clone 9E11) is useful for Flow Cytometry, ChIP, Immunoprecipitation, ELISA, Immunohistochemistry- Frozen and Paraffin and Western blot.
Reviewed Applications
Read 3 Reviews rated 4.7
NB200-108 in the following applications:

Read Publications using
NB200-108 in the following applications:

  • 1 publication
  • 1 publication
  • IP
    2 publications
  • WB
    2 publications

Reactivity Notes

Human, mouse, Saccharomyces and chicken.

Alternate Names for c-Myc Antibody (9E11)

  • avian myelocytomatosis viral oncogene homolog
  • BHLHE39
  • bHLHe39MRTL
  • Class E basic helix-loop-helix protein 39
  • cMyc
  • c-Myc
  • myc proto-oncogene protein
  • Myc
  • Myc2
  • MYCC
  • myc-related translation/localization regulatory factor
  • Niard
  • Nird
  • Proto-oncogene c-Myc
  • Transcription factor p64
  • v-myc avian myelocytomatosis viral oncogene homolog
  • v-myc myelocytomatosis viral oncogene homolog (avian)


Myc genes are a family of proto-oncogenes (L- Myc, N- Myc and C- Myc) that codes for Myc proteins which are transcriptor factors implicated in cellular proliferation, differentiation, apoptosis, metabolism, adhesion and self-renovation of tumor stem cells. Myc protein can act as transcriptional activator/repressor, and is activated via response to diverse mitogenic signals (including Wnt, Shh and EGF) and has been found to be up-regulated in several types of cancers. c-Myc participates gene transcription regulation and binds DNA in a non-specific manner, yet can specifically recognizes core sequence 5'-CAC[GA]TG-3' also. c-Myc heterodimerization with another bHLH protein namely Myc-associated factor X (MAX) is required for efficient c-Myc- DNA binding. c-Myc interacts with several proteins such as TAF1C, SPAG9, PARP10, KDM5A, KDM5B, NO66, PIM2 and with FBXW7 when phosphorylated at Thr-58/Ser-62. c-Myc activate the transcription of growth-related genes and c- Myc overexpression induce cell-cycle progression thereby implicating in a variety of cancers. Moreover, a chromosomal aberration involving c-Myc has been linked to a form of B-cell chronic lymphocytic leukemia and defective c-MYC is responsible for Burkitt lymphoma also.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for c-Myc Antibody (NB200-108)(8)

We have publications tested in 3 confirmed species: Human, Rat, Yeast.

We have publications tested in 4 applications: ChIP, IHC-P, IP, WB.

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Showing Publications 1 - 8 of 8.
Publications using NB200-108 Applications Species
Hubbi ME, Kshitiz, Gilkes DMet al. A nontranscriptional role for HIF-1alpha as a direct inhibitor of DNA replication. Sci Signal 2013 Feb 12 [PMID:23405012]
Matsumura S et al. Activation of the Kaposi's sarcoma-associated herpesvirus major latency locus by the lytic switch protein RTA (ORF50). J Virol 79:8493-505 (2005). [PMID:15956592] (WB, Human) WB Human
Sers JA. Gruppuso PA Nucleolar localization of hepatic c-Myc: a potential mechanism for c-Myc regulation. Biochim Biophys Acta 1743:141-50. 2005 [PMID:15777849] (WB, Rat) WB Rat
Robert F et al. Global position recruitment of HATs HDACs in the yeast genome. Mol Cell 16:199-209. 2004 [PMID:15494307]
Zhang H et al. The Yaf9 component of the SWR1 NuA4 complexes is required for proper gene expression, histone H4 acetylation, Htz1 replacement near telomeres. Mol Cell Biol 24:9424-36. 2004 [PMID:15485911] (ChIP, Yeast) ChIP Yeast
Tanaka S, Diffley JF. Interdependent nuclear accumulation of budding yeast Cdt1 and Mcm2-7 during G1 phase. Nat Cell Biol4 (3):198-207. 2002 Mar. [PMID:11836525] (IP, Yeast) IP Yeast
Pich A, Margaria E, Chiusa L. Oncogenes and male breast carcinoma: c-erbB-2 and p53 coexpression predicts a poor survival. J Clin Oncol 18(16):2948-56. 2000 Aug. [PMID:10944127] (IHC-P, Human) IHC-P Human
Munchow S, Sauter C, Jansen RP. Association of the class V myosin Myo4p with a localised messenger RNA in budding yeast depends on She proteins. J Cell Sci 112 ( Pt 10):1511-8. 1999 May. [PMID:10212145] (IP, Yeast) IP Yeast

Reviews for c-Myc Antibody (NB200-108) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.
We have 3 reviews tested in 2 application: ChIP, WB.

Reviews using NB200-108:
Filter by Applications
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Images Ratings Applications Species Date Details
reviewed by:
ChIP Human 06/27/2012


ApplicationChromatin Immunoprecipitation
Sample TestedHela whole nuclear lysate

Primary Anitbody

Dilution Ratio2ug per reaction


Detection NotesQ-PCR
Fixation/Cross-Linking16 Hrs at 55 Deg followed by 2 Hr treatment with Proteinase K
DNS SharingBiorupter - 40 cycles of 30 secs, final base pair size ~500 bp
Immunoprecipitation DetailsDynabeads Protein A/G (Invirtogen)
Crosslinking/Reversal Details1% Formaldehyde for 10 mins followed by quenching with Ice Cold PBS for 5 mins
DNA Prurification DetailsPhenol-Chloroform extraction
reviewed by:
WB Human 06/20/2012


ApplicationWestern Blot
Sample TestedHEK293 whole cell lysate


Blocking Details3% Milk in PBS for 1 hour at RT

Primary Anitbody

Dilution Ratio1:1000

Secondary Antibody

Secondary Descriptionanti-mouse HRP
Secondary Manufacturer Cat#Jackson Immunoresearch
Secondary Concentration1:5000


Detection NotesECL, 1 mins exposure
reviewed by:
WB Human 08/10/2010


ApplicationWestern Blot
Sample Tested293T Cells overexpressing myc-tagged proteins, Sample Amount: 50
CommentsClear detection of myc-tagged fusion proteins.


Blocking DetailsBlocking Buffer: 5% Milk in PBST, Blocking Time: 1 hour, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius


CommentsClear detection of myc-tagged fusion proteins.

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar

FAQs for c-Myc Antibody (NB200-108). (Showing 1 - 1 of 1 FAQs).

  1. Can I use ant myc antibody for my ChIP since my protein does not have antibody yet? Am going to get a myc-tag cloned protein as transfection-ready for this assay in Caco-2 cell line. How do I start and any tips since and going to use your kit?
    • Yes, you can use c-myc antibody for your mentioned endeavors. c-Myc Antibody (9E11) (NB200-108) is one of our best sellers that has been validated for ChIP application. It has nice customer reviews and has been cited in several peer reviewed publications as well. Regarding your question on tips, can you please confirm me the catalog number of the kit you have or are planning to buy? Moreover, please let me know if you have any specific questions.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB200-108AF405
Alexa Fluor (R) 488 Labeled NB200-108AF488
Alexa Fluor (R) 647 Labeled NB200-108AF647
Alexa Fluor (R) 700 Labeled NB200-108AF700
Allophycocyanin Labeled NB200-108APC
Biotin Labeled NB200-108B
DyLight 350 Labeled NB200-108UV
DyLight 405 Labeled NB200-108V
DyLight 405LS Labeled NB200-108V3
DyLight 488 Labeled NB200-108G
DyLight 550 Labeled NB200-108R
DyLight 650 Labeled NB200-108C
DyLight 680 Labeled NB200-108FR
DyLight 755 Labeled NB200-108IR
FITC Labeled NB200-108F
HRP Labeled NB200-108H
PE Labeled NB200-108PE
PerCP Labeled NB200-108PCP

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Blogs on c-Myc. Showing 1-10 of 12 blog posts - Show all blog posts.

MAPK8/JNK1 - A multifunctional kinase and drug target for cancer therapeutics
The c-Jun N-terminal kinase (JNK) family is a group of regulatory kinases with important functions in cell morphogenesis, inflammation, differentiation, and cell death (1). Aberrant activation of JNK family proteins in cancers has led to interest i...  Read full blog post.

MYC - A human oncogene with valuable laboratory applications
Myc is a basic helix-loop-helix zipper transcription factor that regulates a network of many hundreds of genes. Myc up-regulates the expression of many genes involved in cell growth and proliferation such as ribosome biogenesis and protein synthesi...  Read full blog post.

c-Myc - transcription factor and oncogene
c-Myc is a protein of the Myc family of transcription factors (c-Myc, B-Myc, L-Myc, N-Myc, and s-Myc) encoded by the MYC proto-oncogene. c-Myc was first discovered as the cellular homolog of the retroviral v-Myc oncogene. c-Myc is a transcription...  Read full blog post.

c-Myc. See Myc Run Transcription Regulation
Myc genes (L-Myc, N-Myc and C-Myc) are a family of transcription factors. c-Myc is involved in transcription regulation, apoptosis and cell growth. Mutations in c-Myc have been tied to several cancers. Free sample bonus: Get a free sample on...  Read full blog post.

Beta Catenin Implications for Signaling
The Wnt/beta Catenin signaling pathway plays a critical role in embryonic development, stem cell self-renewal and regeneration. Alterations in this signaling cascade have been implicated in the pathogenesis of cancer. Notably, chronic activation of Wn...  Read full blog post.

Cerebellar Degeneration-Related Protein 2 (CDR2): Cell-Cycle Regulated Tumor Antigen
CDR2 is a tumor antigen expressed in a high percentage of breast and ovarian tumors and is the target of a naturally occurring tumor immune response in patients with paraneoplastic cerebellar degeneration. CDR2 has also been shown to be a cell cycle r...  Read full blog post.

CIP2A: The Cancerous Inhibitor of Protein Phosphatase 2A
The autoantigen p90 is a recently discovered protein that binds to and inhibits Protein Phosphatase 2A (PP2A) activity, thereby playing a critical role in cancer progression. Thus, p90 was renamed the "cancerous inhibitory protein of PP2A" or CIP2A....  Read full blog post.

Nucleolin Antibodies: Knowing When it's Time to Split
Nucleolin is an abundant, 106 kDa nucleolar phosphoprotein that is a major protein in actively dividing cells. The stability of nucleolin is heavily cell proliferation-dependent, as nucleolin antibody studies have shown that degraded forms are relativ...  Read full blog post.

The Myc Epitope Tags Along...
Recombinant DNA technology allows researchers to fuse epitope tags to their protein of interest and then identify that protein using tag specific antibodies. The Myc Tag can be used to purify tagged proteins by affinity chromatography or detect them b...  Read full blog post.

The c-Myc Antibody: A Major Tool in Cancer Research
C-Myc is a widely expressed transcription factor, regulating cellular differentiation, proliferation, cell cycle progression and pro-apoptotic gene expression. The c-Myc antibody is widely used in cancer research, as a number of human tumors have been...  Read full blog post.

Showing 1-10 of 12 blog posts - Show all blog posts.

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Gene Symbol MYC

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