c-Myc Antibody (9E11) Summary
A synthetic peptide corresponding to amino acids 408-420 (AEEQKLISEEDL) of human c-Myc, conjugated to KLH. [UniProt# P01106]
Protein A or G purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
- Western Blot 1:500-1:1000
- Chromatin Immunoprecipitation 2 ug / 500 ug extract
- ELISA 1:100-1:2000
- Flow Cytometry 1:200-1:400
- Immunohistochemistry 1:100
- Immunohistochemistry-Frozen 1:100
- Immunohistochemistry-Paraffin 1:100
- Immunoprecipitation 2ug/mg lysate
This c-Myc antibody (clone 9E11) is useful for Flow Cytometry, ChIP, Immunoprecipitation, ELISA, Immunohistochemistry- Frozen and Paraffin and Western blot.
|Read Publications using
NB200-108 in the following applications:
Human, mouse, Saccharomyces and chicken.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
Protein A or G purified
Alternate Names for c-Myc Antibody (9E11)
- avian myelocytomatosis viral oncogene homolog
- Class E basic helix-loop-helix protein 39
- myc proto-oncogene protein
- myc-related translation/localization regulatory factor
- Proto-oncogene c-Myc
- Transcription factor p64
- v-myc avian myelocytomatosis viral oncogene homolog
- v-myc myelocytomatosis viral oncogene homolog (avian)
Myc genes are a family of proto-oncogenes (L- Myc, N- Myc and C- Myc) that codes for Myc proteins which are transcriptor factors implicated in cellular proliferation, differentiation, apoptosis, metabolism, adhesion and self-renovation of tumor stem cells. Myc protein can act as transcriptional activator/repressor, and is activated via response to diverse mitogenic signals (including Wnt, Shh and EGF) and has been found to be up-regulated in several types of cancers. c-Myc participates gene transcription regulation and binds DNA in a non-specific manner, yet can specifically recognizes core sequence 5'-CAC[GA]TG-3' also. c-Myc heterodimerization with another bHLH protein namely Myc-associated factor X (MAX) is required for efficient c-Myc- DNA binding. c-Myc interacts with several proteins such as TAF1C, SPAG9, PARP10, KDM5A, KDM5B, NO66, PIM2 and with FBXW7 when phosphorylated at Thr-58/Ser-62. c-Myc activate the transcription of growth-related genes and c- Myc overexpression induce cell-cycle progression thereby implicating in a variety of cancers. Moreover, a chromosomal aberration involving c-Myc has been linked to a form of B-cell chronic lymphocytic leukemia and defective c-MYC is responsible for Burkitt lymphoma also.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for c-Myc Antibody (NB200-108). (Showing 1 - 1 of 1 FAQs).
Can I use ant myc antibody for my ChIP since my protein does not have antibody yet? Am going to get a myc-tag cloned protein as transfection-ready for this assay in Caco-2 cell line. How do I start and any tips since and going to use your kit?
- Yes, you can use c-myc antibody for your mentioned endeavors. c-Myc Antibody (9E11) (NB200-108) is one of our best sellers that has been validated for ChIP application. It has nice customer reviews and has been cited in several peer reviewed publications as well. Regarding your question on tips, can you please confirm me the catalog number of the kit you have or are planning to buy? Moreover, please let me know if you have any specific questions.
Positive Control Lysate(s)
Other Available Formats
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Bioinformatics Tool for c-Myc Antibody (NB200-108)
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Read the Bioinformatics Tool Guide
for instructions on using this tool.
Blogs on c-Myc. Showing 1-10 of 14 blog posts - Show all blog posts.
The role of c-Fos in the regulation of the JC virus gene transcription
c-Fos is a member of the AP-1 transcription factor family under the Fos protein family umbrella, alongside Fra-1, Fra-2 and Fos-B. Also in the AP-1 transcription family are the Jun proteins, c-Jun, Jun-B and Jun-D. Each member of the AP-1 transcri... Read full blog post.
Dual applications of a c-Myc antibody in mitochondrial research
c-Myc, a proto-oncogene, has documented involvement in cellular differentiation, cell growth, cell death and tumor formation. Target genes of the Myc family include those that participate in cell survival, translation, transcription, metabolism and... Read full blog post.
MAPK8/JNK1 - A multifunctional kinase and drug target for cancer therapeutics
The c-Jun N-terminal kinase (JNK) family is a group of regulatory kinases with important functions in cell morphogenesis, inflammation, differentiation, and cell death (1). Aberrant activation of JNK family proteins in cancers has led to interest i... Read full blog post.
MYC - A human oncogene with valuable laboratory applications
Myc is a basic helix-loop-helix zipper transcription factor that regulates a network of many hundreds of genes. Myc up-regulates the expression of many genes involved in cell growth and proliferation such as ribosome biogenesis and protein synthesi... Read full blog post.
c-Myc - transcription factor and oncogene
c-Myc is a protein of the Myc family of transcription factors (c-Myc, B-Myc, L-Myc, N-Myc, and s-Myc) encoded by the MYC proto-oncogene. c-Myc was first discovered as the cellular homolog of the retroviral v-Myc oncogene. c-Myc is a transcription... Read full blog post.
c-Myc. See Myc Run Transcription Regulation
Myc genes (L-Myc, N-Myc and C-Myc) are a family of transcription factors. c-Myc is involved in transcription regulation, apoptosis and cell growth. Mutations in c-Myc have been tied to several cancers. Free sample bonus: Get a free sample on... Read full blog post.
Beta Catenin Implications for Signaling
The Wnt/beta Catenin signaling pathway plays a critical role in embryonic development, stem cell self-renewal and regeneration. Alterations in this signaling cascade have been implicated in the pathogenesis of cancer. Notably, chronic activation of Wn... Read full blog post.
Cerebellar Degeneration-Related Protein 2 (CDR2): Cell-Cycle Regulated Tumor Antigen
CDR2 is a tumor antigen expressed in a high percentage of breast and ovarian tumors and is the target of a naturally occurring tumor immune response in patients with paraneoplastic cerebellar degeneration. CDR2 has also been shown to be a cell cycle r... Read full blog post.
CIP2A: The Cancerous Inhibitor of Protein Phosphatase 2A
The autoantigen p90 is a recently discovered protein that binds to and inhibits Protein Phosphatase 2A (PP2A) activity, thereby playing a critical role in cancer progression. Thus, p90 was renamed the "cancerous inhibitory protein of PP2A" or CIP2A.... Read full blog post.
Nucleolin Antibodies: Knowing When it's Time to Split
Nucleolin is an abundant, 106 kDa nucleolar phosphoprotein that is a major protein in actively dividing cells. The stability of nucleolin is heavily cell proliferation-dependent, as nucleolin antibody studies have shown that degraded forms are relativ... Read full blog post.