BLIMP1/PRDM1 Antibody (3H2-E8) [Biotin]

Images

 
Flow Cytometry: Blimp-1 Antibody (3H2-E8) [Biotin] [NB600-235B] - Blimp-1 in mouse spleen. Image courtesy of product review by Branislav Krljanac.

Product Details

Summary
Reactivity Hu, Mu, PoSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Clone
3H2-E8
Clonality
Monoclonal
Host
Mouse

BLIMP1/PRDM1 Antibody (3H2-E8) [Biotin] Summary

Immunogen
A fragment of mouse Blimp-1 corresponding to residues 199-409. [UniProt# Q60636]
Localization
Nuclear
Marker
Plasma Cell Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PRDM1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Conjugate
Biotin
Format
Biotin (A=N/A, E=N/A)
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA 1:100-1:2000
  • Flow Cytometry 8 ug/ml where cells are used at 2*10^6/mL
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
Application Notes
This Blimp-1 (3H2-E8) antibody is useful for ELISA, Immunocytochemistry/Immunofluorescence, Western Blot, Immunohistochemistry on paraffin-embedded sections and Flow Cytometry. Immunohistochemistry-Frozen was reported in scientific literature. By WB, this antibody recognizes a band at ~98 kDa and may recognize one at ~80 kDa (the beta form). Antigen retrieval is recommended (EDTA buffer, microwave) prior to IHC on paraffin tissues. This antibody demonstrates nuclear staining. This antibody does not immunoprecipitate. For IHC and ICC/IF a dilution of 1:200 is recommended with tyramide amplification.
Readout System
Reviewed Applications
Read 1 Review rated 4
using
NB600-235B in the following applications:

Reactivity Notes

Human, mouse and pig.

Alternate Names for BLIMP1/PRDM1 Antibody (3H2-E8) [Biotin]

  • Beta-interferon gene positive regulatory domain I-binding factor
  • beta-interferon gene positive-regulatory domain I binding factor
  • BLIMP-1
  • BLIMP1/PRDM1
  • BLIMP1MGC118925
  • B-lymphocyte-induced maturation protein 1
  • MGC118922
  • MGC118923
  • Positive regulatory domain I-binding factor 1
  • PR domain containing 1, with ZNF domain
  • PR domain zinc finger protein 1
  • PR domain-containing protein 1
  • PRDI-BF1MGC118924
  • PRDI-binding factor 1
  • PRDI-binding factor-1
  • PRDM1
  • PR-domain zinc finger protein 1
  • ZNFPR1A1

Background

Blimp-1 (B lymphocyte maturation protein-1) is a nuclear protein with five zinc fingers in its carboxy terminus. It is a transcriptional repressor that is both required and sufficient to trigger terminal differentiation of B lymphocytes and monocyte/macrophages. Blimp-1 is expressed in, and may be required for terminally differentiated epithelial cells, retinal neurons and other lineage cells. It has also been implicated in the repression of beta-interferon (IFN-beta) and c-myc gene expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BLIMP1/PRDM1 Antibody (NB600-235B) (0)

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Reviews for BLIMP1/PRDM1 Antibody (NB600-235B) (1) 41

Average Rating: 4
(Based on 1 reviews)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Flow.

Reviews using NB600-235B:
Filter by Applications
Flow
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
Flow Cytometry BLIMP1/PRDM1 NB600-235B
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reviewed by:
Anonymous
Flow Mouse 04/27/2012
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Summary

ApplicationFlow Cytometry
Sample TestedMouse Spleen
SpeciesMouse
LotC-C
CommentsBLIMP-1 (3H2-E8) [Biotin] works great for Western blot too.

Blocking

Blocking Details10min Avidin solution (Vector) mixed 1:1 with the 1x PERM buffer (eBioscience) + wash + 10min biotin(Vector) 1:1 w. perm.b.+wash

Primary Anitbody

Dilution RatioBLIMP-1 (3H2-E8) [Biotin] 1:100, 20min, 4 degrees, in 1x PERM buffer (eBioscience)

Details

Detection NotesFACS Canto II, Negative control: BLIMP-1 omitted + SA-PE added
Fixation DetailsSame as above - One step fixation and Permeabilization with the 1x Fix/Perm Foxp3 buffer (eBioscience)
Permeabilization Details1x Fix/Perm Foxp3 buffer (eBioscience) - 45min on 4 degrees
Wash Description2x wash after 1x Fix/Perm buffer, 1x wash after avidin, 1x wash after biotin

Comments

CommentsBLIMP-1 (3H2-E8) [Biotin] works great for Western blot too.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for BLIMP1/PRDM1 Antibody (NB600-235B) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NB600-235APC
DyLight 350 Labeled NB600-235UV
DyLight 405 Labeled NB600-235V
DyLight 405LS Labeled NB600-235V3
DyLight 488 Labeled NB600-235G
DyLight 550 Labeled NB600-235R
DyLight 650 Labeled NB600-235C
DyLight 680 Labeled NB600-235FR
DyLight 755 Labeled NB600-235IR
FITC Labeled NB600-235F
HRP Labeled NB600-235H
PE Labeled NB600-235PE
PerCP Labeled NB600-235PCP

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol PRDM1
Entrez
Uniprot

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