Pax5/BSAP Antibody (PAX5/3735)

Western Blot: Pax5/BSAP Antibody (PAX5/3735) [NBP3-07581] - Western Blot Analysis of Raji cell lysate using Pax5/BSAP Mouse Monoclonal Antibody (PAX5/3735).

Immunohistochemistry-Paraffin: Pax5/BSAP Antibody (PAX5/3735) [NBP3-07581] - Formalin-fixed, paraffin-embedded human Tonsil stained with Pax5/BSAP Mouse Monoclonal Antibody (Pax5/BSAP/3735).

Immunohistochemistry-Paraffin: Pax5/BSAP Antibody (PAX5/3735) [NBP3-07581] - Formalin-fixed, paraffin-embedded human Tonsil stained with Pax5/BSAP Mouse Monoclonal Antibody (Pax5/BSAP/3735).

Protein Array: Pax5/BSAP Antibody (PAX5/3735) [NBP3-07581] - Analysis of Protein Array containing more than 21,000 full-length human proteins using Pax5/BSAP Mouse Monoclonal Antibody (Pax5/BSAP/3735).

Immunohistochemistry-paraffin: Mouse Monoclonal Pax5/BSAP Antibody (PAX5/3735) [NBP3-07581] - Pax5/BSAP immunoreactivity in a dog lymph node. Primary was used at 0.5ug/mL and was incubated with the tissue section for 30m at room temperature. Secondary was Horse Anti-Mouse HRP polymer. Image from a verified customer review.

Immunohistochemistry-paraffin: Mouse Monoclonal Pax5/BSAP Antibody (PAX5/3735) [NBP3-07581] - Pax5/BSAP immunoreactivity in equine skin. Primary was used at 0.5ug/mL and was incubated with the tissue section for 30m at room temperature. Secondary was Horse Anti-Mouse HRP polymer. Image from a verified customer review.

Immunohistochemistry-paraffin: Mouse Monoclonal Pax5/BSAP Antibody (PAX5/3735) [NBP3-07581] - Pax5/BSAP immunoreactivity in human tonsil. Primary was used at 0.5ug/mL and was incubated with the tissue section for 30m at room temperature. Secondary was Horse Anti-Mouse HRP polymer. Image from a verified customer review.

Immunohistochemistry-paraffin: Mouse Monoclonal Pax5/BSAP Antibody (PAX5/3735) [NBP3-07581] - Pax5/BSAP immunoreactivity in feline intestine. Primary was used at 0.5ug/mL and was incubated with the tissue section for 30m at room temperature. Secondary was Horse Anti-Mouse HRP polymer. Image from a verified customer review.

Immunohistochemistry-Frozen: Mouse Monoclonal Pax5/BSAP Antibody (PAX5/3735) [NBP3-07581] - Immunoreactivity was observed with this Pax5 antibody (red) in Rhesus macaque spleen tissues. Nuclei counterstained with DAPI (blue). Primary antibody dilution used: 1:100. Image from a verified customer review.

Formalin-fixed, paraffin-embedded human Tonsil stained with Pax5/BSAP Antibody (PAX5/3735).

Formalin-fixed, paraffin-embedded human Tonsil stained with Pax5/BSAP Antibody (PAX5/3735).

• Reactivity: Hu, Ca, Eq, Fe, RM
• Applications: WB, IHC, MA
• Clone: PAX5/3735
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Pax5/BSAP Antibody (PAX5/3735) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08418)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: A recombinant fragment (around aa 235-382) of human PAX5 protein (exact sequence is proprietary) (Uniprot: Q02548)
• Localization: Nuclear
• Marker: Early B-Cell Marker
• Specificity: The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 21,000, full-length human proteins. The PAX5 gene is a member of the paired box (PAX)family of transcription factors. The central feature of this gene family is a novel, highly conservedDNA-binding domain, known as the paired box. The PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute toneoplastic transformation. The PAX5 gene encodes theB-celllineage specific activator protein (BSAP) that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis; therefore, PAX5 gene product may not only play an important role in B-cell differentiation, but also in neural development and spermatogenesis.
• Isotype: IgG1 Lambda
• Clonality: Monoclonal
• Host: Mouse
• Gene: PAX5
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Frozen Validated for Immunohistochemistry-Frozen from a verified customer review.
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
  • Western Blot
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Feline and Equine reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Pax5/BSAP Antibody (PAX5/3735)

• B cell specific activator protein
• B-cell-specific transcription factor
• BSAP
• BSAPB-cell lineage specific activator
• paired box 5
• paired box gene 5 (B-cell lineage specific activator protein)
• paired box gene 5 (B-cell lineage specific activator)
• paired box homeotic gene 5
• paired box protein Pax-5
• Pax5
• transcription factor PAX 5

Background

PAX5 encodes a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding motif, known as the paired box. PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. This gene encodes the B-cell lineage specific activator protein that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis and so the encoded protein may also play a role in neural development and spermatogenesis. This gene is located at 9p13, which is involved in t(9;14)(p13;q32) translocations recurring in small lymphocytic lymphomas of the plasmacytoid subtype, and in derived large-cell lymphomas. This translocation brings the potent E-mu enhancer of the IgH gene into close proximity of the PAX5 promoter, suggesting that the deregulation of transcription of this gene contributes to the pathogenesis of these lymphomas. Alternatively spliced transcript variants encoding different isoforms have been described but their biological validity has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Reviews for Pax5/BSAP Antibody (NBP3-07581) (5)

Average Rating: 5

(Based on 5 reviews)

We have 5 reviews tested in 5 species: Human, Canine, Equine, Feline, Rhesus Macaque.

reviewed by: Verified Customer IHC-Fr Rhesus Macaque 04/18/2024

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: spleen
• Species: Rhesus Macaque
• Lot: 5079-6P231220
• Pub Med ID: ***Novus Innovators Program - new species or application used on a primary antibody.***

reviewed by: Verified Customer IHC-P Feline 10/13/2023

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Adult intestine,Intestine
• Species: Feline
• Lot: 5079-6P230620

Comments

• Comments: ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.*** Requires heat-induced epitope retrieval.

reviewed by: Verified Customer IHC-P Human 10/13/2023

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: human tonsil
• Species: Human
• Lot: 5079-6P230620

reviewed by: Verified Customer IHC-P Equine 10/13/2023

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Skin
• Species: Equine
• Lot: 5079-6P230620

Comments

• Comments: ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***

reviewed by: Verified Customer IHC-P Canine 10/13/2023

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Lymph Node
• Species: Canine
• Lot: 5079-6P230620

Comments

• Comments: Requires heat-induced epitope retrieval. I found that both citrate and EDTA buffer works, but the signal is better with EDTA.

Bioinformatics

• Gene Symbol: PAX5
• Uniprot:
  • Q02548()
  • Q02548()