AICDA Antibody

Western Blot: AICDA Antibody [NB100-93454] - Staining of Human Tonsil lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

• Reactivity: Hu, Mu, Po, Bv
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

AICDA Antibody Summary

• Description: Novus Biologicals Goat AICDA Antibody (NB100-93454) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-AICDA Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence RRKFLYQFKNVR-C corresponding to N-Terminus according to NP_065712.1.
• Predicted Species: Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: AICDA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Peptide ELISA Detection limit 1:1000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 26 kDa band observed in human lymph node, spleen and tonsil lysates (calculated MW of 24.0 kDa band according to NP_065712.1). Use in ICC/IF reported in scientific literature (PMID:34450044).
• Control:

  AICDA Overexpression Lysate

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:34450044).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for AICDA Antibody

• activation-induced cytidine deaminase
• AICDA
• AID
• AIDCytidine aminohydrolase
• Arp2
• CDA2
• HIGM2
• HIGM2EC 3.5.4.5
• integrated into Burkitt's lymphoma cell line Ramos

Background

Activation-induced cytidine deaminase (AID) was initially discovered as a homolog of the apolipoprotein B RNA-editing cytidine deaminase 1 (APOBEC1) that showed cytidine deaminase properties in stimulated B cell lines. It is necessary for somatic hypermutation and class switch recombination in B cells, but inappropriate or dysregulated expression AID is often found in tumors and B cell neoplasms. Although it is structurally and functionally similar to the APOBEC proteins, it appears unlikely that AID deaminates dC to dU residues in HIV cDNA as does APOBEC3G.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AICDA Antibody (NB100-93454)(2)

Publications using NB100-93454

• Nair L, Zhang W, Laffleur B Et al. Mechanism of noncoding RNA-associated N6-methyladenosine recognition by an RNA processing complex during IgH DNA recombination Molecular cell 2021-08-20 [PMID: 34450044] (ICC/IF, Mouse)
• Crouch EE, Li Z, Takizawa M et al. Regulation of AID expression in the immune response. J Exp Med 2007-05-14 [PMID: 17452520]

Bioinformatics

• Gene Symbol: AICDA
• Entrez:
  • 57379(Human)
• Uniprot:
  • P00441()