Mouse IL-5 Quantikine ELISA Kit, 2 Plate Summary
The Quantikine Mouse IL-5 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-5
in cell culture supernates and mouse serum. It contains Sf 21-expressed recombinant mouse IL-5 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant mouse IL-5. Results obtained using natural mouse IL-5 showed linea ... r curves that were parallel to the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-5.
Natural and recombinant mouse IL-5
Solid Phase Sandwich ELISA
No significant interference observed with available related molecules.
Packaging, Storage & Formulations
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Mouse IL-5 Quantikine ELISA Kit, 2 Plate
BCDF mu B-cell differentiation factor I BCGFII EDF Eo-CSF Eosinophil differentiation factor IL5 IL-5 IL-5T-cell replacing factor interleukin 5 (colony-stimulating factor, eosinophil) interleukin-5 TRF TRFB cell differentiation factor I Background
IL-5 (Interleukin-5) is a secreted cytokine that is expressed as a covalent dimer. It is produced by activated Th2 cells, eosinophils, mast cells, and iNKT cells and promotes eosinophilic and basophilic differentiation and activation. IL-5 signals through a receptor complex containing IL-5 R alpha and the Common beta Chain/CD131 on eosinophils and basophils. Naturally occurring soluble forms of IL-5 R alpha retain the ability to bind IL-5 and limit its bioavailability.
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Applications: Flow, ICC/IF, IHC, IHC-P, WB
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Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
Species: Mu, Rt
Applications: Cell Depl, CyTOF-ready, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, InhibTFunc
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.
Publications for IL-5 (M5000)(78)
We have publications tested in 3 confirmed species: Human, Mouse, Rat.
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Publications using M5000
Y Lu, Y Huang, J Li, J Huang, L Zhang, J Feng, J Li, Q Xia, Q Zhao, L Huang, S Jiang, S Su Eosinophil extracellular traps drive asthma progression through neuro-immune signals Nature Cell Biology, 2021;23(10):1060-1072. 2021 [PMID: 34616019] (Mouse)
S Lee, A Ishitsuka, T Kuroki, YH Lin, A Shibuya, T Hongu, Y Funakoshi, Y Kanaho, K Nagata, A Kawaguchi Arf6 exacerbates allergic asthma through cell-to-cell transmission of ASC inflammasomes JCI Insight, 2021;6(16):. 2021 [PMID: 34423792] (Mouse)
W Wei, W Chen, N He HDAC4 induces the development of asthma by increasing Slug-upregulated CXCL12 expression through KLF5 deacetylation Journal of Translational Medicine, 2021;19(1):258. 2021 [PMID: 34118928] (Mouse)
LT Wang, MH Lin, KY Liu, SS Chiou, SN Wang, CY Chai, LW Tseng, HC Chiou, HC Wang, KK Yokoyama, SH Hsu, SK Huang WLS/wntless is essential in controlling dendritic cell homeostasis via a WNT signaling-independent mechanism Autophagy, 2021;0(0):1-16. 2021 [PMID: 33853474] (Mouse)
Á Rol, T Todorovski, P Martin-Mal, A Escolà, E Gonzalez-R, E Aragón, X Verdaguer, M Vallès-Mir, J Farrera-Si, E Puig, J Fernández-, B Ponsati, M Delgado, A Riera, MJ Macias Structure-based design of a Cortistatin analogue with immunomodulatory activity in models of inflammatory bowel disease Nature Communications, 2021;12(1):1869. 2021 [PMID: 33767180] (Mouse)
E Saba, YY Lee, MH Rhee, SD Kim Alleviation of Ulcerative Colitis Potentially through th1/th2 Cytokine Balance by a Mixture of Rg3-enriched Korean Red Ginseng Extract and Persicaria tinctoria Molecules, 2020;25(22):. 2020 [PMID: 33182623] (Mouse)
W Zhou, J Zhang, S Toki, K Goleniewsk, AE Norlander, DC Newcomb, P Wu, KL Boyd, H Kita, RS Peebles COX Inhibition Increases Alternaria-Induced Pulmonary Group 2 Innate Lymphoid Cell Responses and IL-33 Release in Mice J. Immunol., 2020;0(0):. 2020 [PMID: 32690653] (Mouse)
CT Wu, FH Lin, YT Lee, MS Ku, KH Lue Effect of Lactobacillus rhamnosus GG immunopathologic changes in chronic mouse asthma model J Microbiol Immunol Infect, 2019;0(0):. 2019 [PMID: 30952512] (Mouse)
I Sulaiman, K Tan, N Mohtarrudi, JC Woei Lim, J Stanslas Andrographolide prevented toluene diisocyanate-induced occupational asthma and aberrant airway E-cadherin distribution via p38 MAPK-dependent Nrf2 induction Pulm Pharmacol Ther, 2018;0(0):. 2018 [PMID: 30244166] (Mouse)
Z Dong, Y Yuan Accelerated inflammation and oxidative stress induced by LPS in acute lung injury: ?nhibition by ST1926 Int. J. Mol. Med., 2018;0(0):. 2018 [PMID: 29568857] (Mouse)
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