Syndecan-1/CD138 Antibody (B-A38)

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Flow Cytometry: Syndecan-1/CD138 Antibody (B-A38) [NB100-64980] - Staining of U266 cell line with MOUSE ANTI HUMAN CD138: FITC.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
B-A38
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.1 mg/ml

Order Details

Syndecan-1/CD138 Antibody (B-A38) Summary

Immunogen
U266 cell line
Localization
Membrane; Single-pass type I membrane protein.
Specificity
NB100-64980 recognizes a heparan sulfate rich membrane glycoprotein of 200kD known as Syndecan-1 (CD138). Syndecan functions as a receptor for collagen, fibronectin and thrombospondin. NB100-64980 stains all plasma cells and plasma cell lines. No staining of other leucocytes is seen in peripheral blood or bone marrow, although some ALL cell lines express the antigen weakly. Some staining of epithelial and endothelial cells may be seen in immunohistology.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SDC1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry Neat
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
Application Notes
For Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. For IHC: This product requires heat pre-treatment. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Control
Syndecan-1/CD138 Lysate (NBP2-64798)
Publications
Read Publications using
NB100-64980 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Phosphate buffered saline
Preservative
0.09% Sodium Azide
Concentration
0.1 mg/ml
Purity
Protein G purified

Alternate Names for Syndecan-1/CD138 Antibody (B-A38)

  • CD138 antigen
  • CD138
  • SDC
  • SDC1
  • SYND1heparan sulfate proteoglycan fibroblast growth factor receptor
  • syndecan 1
  • syndecan proteoglycan 1
  • syndecan
  • Syndecan1
  • Syndecan-1

Background

CD138 / Syndecan 1 is a transmembrane heparin sulphate proteoglycan which is made up of one core protein and five glycosaminoglycan. CD138 is expected to play a role in cell adhesion. It is expressed on the surface of pre B cells and plasma cells but is absent from mature B cells. It is a selective marker for B cell lymphoblastic leukemia and lymphoplasmocytoid leukemia. It is lost from the apoptotic myeloma cells; hence is a useful marker for viable myeloma cells. This antibody stains all plasma cells and plasma cell lines. No staining of other leucocytes is seen in peripheral blood or bone marrow, although some ALL cell lines express the antigen weakly. Some staining of epithelial and endothelial cells may be seen in immunohistology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Syndecan-1/CD138 Antibody (NB100-64980)(16)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC/IF.


Filter By Application
ICC/IF
(1)
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Filter By Species
Human
(1)
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Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using NB100-64980 Applications Species
Sharpe B, Alghezi DA, Cattermole C et al. A subset of high Gleason grade prostate carcinomas contain a large burden of prostate cancer syndecan-1 positive stromal cells Prostate 2017 Jul 26 [PMID: 28744948] (ICC/IF, Human) ICC/IF Human
Li, K et al. Anaplastic lymphoma kinase-positive diffuse large B-cell lymphoma presenting as an isolated nasopharyngeal mass: a case report and review of literature. Int J Clin Exp Pathol 4: 190-6. 2010 [PMID: 21326807]
Beauvais, DM Rapraeger, AC. Syndecan-1 couples the insulin-like growth factor-1 receptor to inside-out integrin activation. J Cell Sci 123: 3796-807. 2010 [PMID: 20971705]
Thaunat, O et al. Chronic rejection triggers the development of an aggressive intragraft immune response through recapitulation of lymphoid organogenesis. J Immunol 185: 717-28. 2010 [PMID: 20525884]
Du, S et al. Systemic mastocytosis in association with chronic lymphocytic leukemia and plasma cell myeloma. Int J Clin Exp Pathol 3: 448-57. 2010 [PMID: 20490336]
Chang, H et al. CKS1B nuclear expression is inversely correlated with p27Kip1 expression and is predictive of an adverse survival in patients with multiple myeloma. 20421271 Haematologica. 95: 1542-7. 2010 [PMID: 20421271]
Kim, YC et al. Presence of Porphyromonas gingivalis and plasma cell dominance in gingival tissues with periodontitis. Oral Dis 16: 375-81. 2010 [PMID: 20233323]
Kylanpaa, L et al. Syndecan-1 and tenascin expression in cystic tumors of the pancreas. JOP 10: 378-82. 2009 [PMID: 19581738]
Beauvais, DM et al. Syndecan-1 regulates alphavbeta3 and alphavbeta5 integrin activation during angiogenesis and is blocked by synstatin, a novel peptide inhibitor. J Exp Med 206: 691-705. 2009 [PMID: 19255147]
Mahshid, Y et al. High expression of 5-lipoxygenase in normal and malignant mantle zone B lymphocytes. BMC Immunol;10:2. 2009 Jan 9. [PMID: 19134178]
Show All 16 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Syndecan-1/CD138 Antibody (NB100-64980). (Showing 1 - 2 of 2 FAQs).

  1. Does this product work on endometrial tissue?
    • Syndecan-1/CD138 antibody (NB100-64980) is a great antibody and it should work in any sections as long as they have CD138 antigen. As far as endometrial tissue is concerned, I am assuming that the cancerous endometrial tissue express Syndecan-1/CD138 while the normal tissue does not express it that much. And we would suggest the customer to include a positive control section in the assay using any of the following tissues: Normal Tissues:- Placenta, Colon, Small Intestine or Tonsil; Cancerous Tissues:- Ovarian cancer, rectal cancer, Lung cancer.
  2. Is this differ from anti human syndecan-1 mouse B-B4 monoclonal antibody?
    • Our NB100-64980 is Syndecan-1/CD138 antibody’s clone B-A38 and this clone is similar to clone B-B4. Both of these clones are IgG1 monoclonal antibodies generated in mouse using U266 myeloma cell line as immunogen. Both of these clones recognize CD138 antigen/ Syndecan-1 protein selectively on Plasma cells /B Cells and are routinely utilized for the diagnosis of multiple myeloma. We do not have license to sell Clone B-B4 at the moment, therefore, the customer can buy our Syndecan-1/CD138 antibody (clone B-A38) NB100-64980 which is a similar product by nature and performance in immunoassays.

Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol SDC1
Entrez
Uniprot