XBP1 Antibody - BSA Free

Western Blot: XBP1 Antibody [NBP1-77681] - Total protein from HeLa cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-XBP1 in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Arrow delineates XBP1s and arrowhead XBP1u.

Immunocytochemistry/Immunofluorescence: XBP1 Antibody [NBP1-77681] - Mouse MS1 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with XBP1 Antibody (NBP1-77681) at 1 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry: XBP1 Antibody [NBP1-77681] - Staining of XBP1 in mouse bladder using DAB with hematoxylin counterstain.

Flow Cytometry: XBP1 Antibody [NBP1-77681] - An intracellular stain was performed on NIH3T3 cells with XBP1 Antibody NBP1-77681 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Immunocytochemistry/Immunofluorescence: XBP1 Antibody [NBP1-77681] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with XBP1 Antibody (NBP1-77681) at 1 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Flow Cytometry: XBP1 Antibody [NBP1-77681] - An intracellular stain was performed on HepG2 cells with XBP1 Antibody NBP1-77681 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Western Blot: XBP1 Antibody [NBP1-77681] - Western Blot of PC-3 cell lysate. Image from verified customer review.

• Reactivity: Hu, Mu
• Applications: WB, Flow, ICC/IF, IHC, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

XBP1 Antibody - BSA Free Summary

• Immunogen: A genomic peptide made to an internal region of the human XBP1 protein (within residues 100-250). [Swiss-Prot P17861]
• Localization: Nucleus
• Specificity: This antibody is specific for both XBP1s and XBP1u.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: XBP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry reported in scientific literature (PMID 31031094)
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Knockdown Validated reported in scientific literature (PMID 33513694)
  • Western Blot 1 ug/mL
• Application Notes: Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for XBP1 Antibody - BSA Free

• HTF
• TREB5
• X-box binding protein 1
• X-box-binding protein 1
• XBP1
• XBP-1
• XBP1s
• XBP1u
• XBP2
• XBP2Tax-responsive element-binding protein 5

Background

XBP1 (X-box binding protein 1 or TREB5), is a unique bZIP transcription factor whose active form is generated by a non-conventional splicing reaction upon disruption of homeostasis in endoplasmic reticulum (ER) and unfolded protein response (UPR) activation. Originally identified as a key regulator of MHC class II gene expression in B cells, XBP1 now is known as the most conserved signaling component of UPR and is critical for cell fate determination in response to ER stress. XBP1 is a nuclear transcription factor essential for hepatocyte growth, plasma cells differentiation, immunoglobulin secretion, and UPR cascade. XBP1 acts upon ER stress by activating UPR target genes via direct binding to UPRE (the UPR element). XBP1 binds DNA preferably to the CRE-like element 5'-GATGACGTG[TG]N3[AT]T-3', and also to certain TPA response elements. XBP1 also binds to HLA DR-alpha promoter as well as to the Tax-responsive element of HTLV-I. XBP1 is up-regulated by ATF6 via direct binding to ERSE in response to ER stress. Genetic variations in XBP1 have been linked with susceptibility to major affective disorder type 7 (MAFD7).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for XBP1 Antibody (NBP1-77681)(10)

Publications using NBP1-77681

• Lombardi S, Goldman AR, Tang HY et al. Targeting Fatty Acid Reprogramming Suppresses CARM1-expressing Ovarian Cancer Cancer research communications 2023-06-01 [PMID: 37377614] (ChIP, Human)
• Pandit M, Kil YS, Ahn JH et al. Methionine consumption by cancer cells drives a progressive upregulation of PD-1 expression in CD4 T cells Nature communications 2023-05-05 [PMID: 37147330] (WB, Mouse)
  
  Details:
  WB 1:1000
• Lin J, Liu H, Fukumoto T et al. Targeting the IRE1 alpha /XBP1s pathway suppresses CARM1-expressing ovarian cancer Nature communications 2021-09-07 [PMID: 34493732] (WB, Human)
• Al-Yacoub N, Colak D, Mahmoud Sa Et Al. Mutation in FBXO32 causes dilated cardiomyopathy through up-regulation of ER-stress mediated apoptosis Communications biology 2021-07-16 [PMID: 34272480]
• Pandey R, Bakay M, Strenkowski BP et al. JAK/STAT inhibitor therapy partially rescues the lipodystrophic autoimmune phenotype in Clec16a KO mice Scientific reports 2021-04-01 [PMID: 33795715] (WB, Mouse)
• Garufi A, Federici G, Gilardini Montani Ms et Al. Interplay between Endoplasmic Reticulum (ER) Stress and Autophagy Induces Mutant p53H273 Degradation Biomolecules 2020-03-03 [PMID: 32138264] (WB, Human)
• Ma, X;Bi, E;Lu, Y;Su, P;Huang, C;Liu, L;Wang, Q;Yang, M;Kalady, MF;Qian, J;Zhang, A;Gupte, AA;Hamilton, DJ;Zheng, C;Yi, Q; Cholesterol Induces CD8+ T Cell Exhaustion in the Tumor Microenvironment Cell Metab. 2019-04-18 [PMID: 31031094] (FLOW, Mouse)
• Ciccia F, Accardo-Palumbo A, Rizzo A et al. Evidence that autophagy, but not the unfolded protein response, regulates the expression of IL-23 in the gut of patients with ankylosing spondylitis and subclinical gut inflammation. Ann Rheum Dis 2013-06-05 [PMID: 23740229]
• Venkatesh P, Kumar S, Kumar N, et al. Molecular mechanisms underlying attenuation of live attenuated Japanese encephalitis virus vaccine SA14-14-2 bioRxiv 2021-01-15 (WB, Porcine, Mouse)
• Gonnella R, Gilardini Montani MS, Guttieri L et al. IRE1 Alpha/XBP1 Axis Sustains Primary Effusion Lymphoma Cell Survival by Promoting Cytokine Release and STAT3 Activation Biomedicines 2021-01-27 [PMID: 33513694] (KD, Human)

Review for XBP1 Antibody (NBP1-77681) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Armen Petrosyan WB Human 06/27/2023

Summary

• Application: Western Blot
• Sample Tested: PC-3 Cells
• Species: Human
• Lot: D104146-2

Bioinformatics

• Gene Symbol: XBP1
• Entrez:
  • 7494(Human)
  • 22433(Mouse)
• Uniprot:
  • P17861(Human)
  • O35426(Mouse)