XBP1 Antibody


Western Blot: XBP1 Antibody [NBP1-77681] - Total protein from HeLa cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ...read more
Immunocytochemistry/ Immunofluorescence: XBP1 Antibody [NBP1-77681] - XBP1 antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).
Immunohistochemistry: XBP1 Antibody [NBP1-77681] - Staining of XBP1 in mouse bladder using DAB with hematoxylin counterstain.

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
1.0 mg/ml

XBP1 Antibody Summary

A genomic peptide made to an internal region of the human XBP1 protein (within residues 100-250). [Swiss-Prot P17861]
This antibody is specific for both XBP1s and XBP1u.
Immunogen affinity purified
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  • Western Blot 1 ug/mL
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
Application Notes
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in FLOW reported in scientific literature (PMID: 31031094).
Read Publications using
NBP1-77681 in the following applications:

  • 1 publication
  • WB
    1 publication

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS, 30% Glycerol
0.05% Sodium Azide
1.0 mg/ml
Immunogen affinity purified


Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for XBP1 Antibody

  • HTF
  • TREB5
  • X-box binding protein 1
  • X-box-binding protein 1
  • XBP1
  • XBP-1
  • XBP1s
  • XBP1u
  • XBP2
  • XBP2Tax-responsive element-binding protein 5


XBP1 (X-box binding protein 1 or TREB5), is a unique bZIP transcription factor whose active form is generated by a non-conventional splicing reaction upon disruption of homeostasis in endoplasmic reticulum (ER) and unfolded protein response (UPR) activation. Originally identified as a key regulator of MHC class II gene expression in B cells, XBP1 now is known as the most conserved signaling component of UPR and is critical for cell fate determination in response to ER stress. XBP1 is a nuclear transcription factor essential for hepatocyte growth, plasma cells differentiation, immunoglobulin secretion, and UPR cascade. XBP1 acts upon ER stress by activating UPR target genes via direct binding to UPRE (the UPR element). XBP1 binds DNA preferably to the CRE-like element 5'-GATGACGTG[TG]N3[AT]T-3', and also to certain TPA response elements. XBP1 also binds to HLA DR-alpha promoter as well as to the Tax-responsive element of HTLV-I. XBP1 is up-regulated by ATF6 via direct binding to ERSE in response to ER stress. Genetic variations in XBP1 have been linked with susceptibility to major affective disorder type 7 (MAFD7).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for XBP1 Antibody (NBP1-77681)(3)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for XBP1 Antibody (NBP1-77681) (0)

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Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-77681AF350
Alexa Fluor 405 NBP1-77681AF405
Alexa Fluor 488 NBP1-77681AF488
Alexa Fluor 532 NBP1-77681AF532
Alexa Fluor 594 NBP1-77681AF594
Alexa Fluor 647 NBP1-77681AF647
Alexa Fluor 700 NBP1-77681AF700
Alexa Fluor 750 NBP1-77681AF750
Biotin NBP1-77681B
DyLight 350 NBP1-77681UV
DyLight 405 NBP1-77681V
DyLight 488 NBP1-77681G
DyLight 550 NBP1-77681R
DyLight 594 NBP1-77681DL594
DyLight 650 NBP1-77681C
DyLight 680 NBP1-77681FR
DyLight 755 NBP1-77681IR
FITC NBP1-77681F
HRP NBP1-77681H
Janelia Fluor 549 NBP1-77681JF549
Janelia Fluor 646 NBP1-77681JF646

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Blogs on XBP1.

Analysis of Total & pSer724 IRE1 alpha, the Sensor of ER Stress
Inositol-requiring protein 1/IRE1 alpha (also called Endoplasmic Reticulum to Nucleus Signaling 1/ERN1; predicted mol wt 110 kDa) is a serine-threonine protein kinase/endoribonuclease which plays a highly critical role in unfolded protein response/U...  Read full blog post.

IRE1 - an important sensor in the unfolded protein response pathway
During cellular stress the protein folding capacity of the ER is diminished. In order to maintain homeostasis and ensure proper protein folding cells activate the unfolded protein response (UPR), a signaling network consisting of sensors and effect...  Read full blog post.

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Gene Symbol XBP1