XBP1 Antibody - BSA Free

Images

 
Western Blot: XBP1 Antibody [NBP1-77681] - Total protein from HeLa cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ...read more
Immunocytochemistry/ Immunofluorescence: XBP1 Antibody [NBP1-77681] - Mouse MS1 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Immunohistochemistry: XBP1 Antibody [NBP1-77681] - Staining of XBP1 in mouse bladder using DAB with hematoxylin counterstain.
Flow Cytometry: XBP1 Antibody [NBP1-77681] - An intracellular stain was performed on NIH3T3 cells with XBP1 Antibody NBP1-77681 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and ...read more
Immunocytochemistry/ Immunofluorescence: XBP1 Antibody [NBP1-77681] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated ...read more
Flow Cytometry: XBP1 Antibody [NBP1-77681] - An intracellular stain was performed on HepG2 cells with XBP1 Antibody NBP1-77681 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

XBP1 Antibody - BSA Free Summary

Immunogen
A genomic peptide made to an internal region of the human XBP1 protein (within residues 100-250). [Swiss-Prot P17861]
Localization
Nucleus
Specificity
This antibody is specific for both XBP1s and XBP1u.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
XBP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Knockdown Validated
  • Western Blot 1 ug/mL
Application Notes
Use in KD reported in scientific literature (PMID:33513694) Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in FLOW reported in scientific literature (PMID: 31031094).
Publications
Read Publications using
NBP1-77681 in the following applications:

  • 1 publication
  • KD
    1 publication
  • WB
    4 publications

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for XBP1 Antibody - BSA Free

  • HTF
  • TREB5
  • X-box binding protein 1
  • X-box-binding protein 1
  • XBP1
  • XBP-1
  • XBP1s
  • XBP1u
  • XBP2
  • XBP2Tax-responsive element-binding protein 5

Background

XBP1 (X-box binding protein 1 or TREB5), is a unique bZIP transcription factor whose active form is generated by a non-conventional splicing reaction upon disruption of homeostasis in endoplasmic reticulum (ER) and unfolded protein response (UPR) activation. Originally identified as a key regulator of MHC class II gene expression in B cells, XBP1 now is known as the most conserved signaling component of UPR and is critical for cell fate determination in response to ER stress. XBP1 is a nuclear transcription factor essential for hepatocyte growth, plasma cells differentiation, immunoglobulin secretion, and UPR cascade. XBP1 acts upon ER stress by activating UPR target genes via direct binding to UPRE (the UPR element). XBP1 binds DNA preferably to the CRE-like element 5'-GATGACGTG[TG]N3[AT]T-3', and also to certain TPA response elements. XBP1 also binds to HLA DR-alpha promoter as well as to the Tax-responsive element of HTLV-I. XBP1 is up-regulated by ATF6 via direct binding to ERSE in response to ER stress. Genetic variations in XBP1 have been linked with susceptibility to major affective disorder type 7 (MAFD7).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-778
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NBP1-77681
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, KD

Publications for XBP1 Antibody (NBP1-77681)(8)

We have publications tested in 3 confirmed species: Human, Mouse, Porcine.

We have publications tested in 3 applications: Flow, KD, WB.


Filter By Application
Flow
(1)
KD
(1)
WB
(4)
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Human
(3)
Mouse
(3)
Porcine
(1)
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Showing Publications 1 - 8 of 8.
Publications using NBP1-77681 Applications Species
Lin J, Liu H, Fukumoto T et al. Targeting the IRE1 alpha /XBP1s pathway suppresses CARM1-expressing ovarian cancer Nature communications Sep 7 2021 [PMID: 34493732] (WB, Human) WB Human
Garufi A, Federici G, Gilardini Montani Ms et Al. Interplay between Endoplasmic Reticulum (ER) Stress and Autophagy Induces Mutant p53H273 Degradation Biomolecules Mar 3 2020 [PMID: 32138264] (WB, Human) WB Human
Ma, X;Bi, E;Lu, Y;Su, P;Huang, C;Liu, L;Wang, Q;Yang, M;Kalady, MF;Qian, J;Zhang, A;Gupte, AA;Hamilton, DJ;Zheng, C;Yi, Q; Cholesterol Induces CD8+ T Cell Exhaustion in the Tumor Microenvironment Cell Metab. Apr 18 2019 [PMID: 31031094] (Flow, Mouse) Flow Mouse
Ciccia F, Accardo-Palumbo A, Rizzo A et al. Evidence that autophagy, but not the unfolded protein response, regulates the expression of IL-23 in the gut of patients with ankylosing spondylitis and subclinical gut inflammation. Ann Rheum Dis 2013 Jun 5 [PMID: 23740229]
Venkatesh P, Kumar S, Kumar N, et al. Molecular mechanisms underlying attenuation of live attenuated Japanese encephalitis virus vaccine SA14-14-2 bioRxiv Jan 15 2021 (WB, Mouse, Porcine) WB Mouse, Porcine
Al-Yacoub N, Colak D, Mahmoud Sa Et Al. Mutation in FBXO32 causes dilated cardiomyopathy through up-regulation of ER-stress mediated apoptosis Communications biology Jul 16 2021 [PMID: 34272480]
Pandey R, Bakay M, Strenkowski BP et al. JAK/STAT inhibitor therapy partially rescues the lipodystrophic autoimmune phenotype in Clec16a KO mice Scientific reports Apr 1 2021 [PMID: 33795715] (WB, Mouse) WB Mouse
Gonnella R, Gilardini Montani MS, Guttieri L et al. IRE1 Alpha/XBP1 Axis Sustains Primary Effusion Lymphoma Cell Survival by Promoting Cytokine Release and STAT3 Activation Biomedicines Jan 27 2021 [PMID: 33513694] (KD, Human) KD Human

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for XBP1 Antibody (NBP1-77681) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on XBP1.

Analysis of Total & pSer724 IRE1 alpha, the Sensor of ER Stress
Inositol-requiring protein 1/IRE1 alpha (also called Endoplasmic Reticulum to Nucleus Signaling 1/ERN1; predicted mol wt 110 kDa) is a serine-threonine protein kinase/endoribonuclease which plays a highly critical role in unfolded protein response/U...  Read full blog post.

IRE1 - an important sensor in the unfolded protein response pathway
During cellular stress the protein folding capacity of the ER is diminished. In order to maintain homeostasis and ensure proper protein folding cells activate the unfolded protein response (UPR), a signaling network consisting of sensors and effect...  Read full blog post.

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Bioinformatics

Gene Symbol XBP1
Entrez
Uniprot