IRF4 Antibody

Images

 
Western blot shows lysates of Ramos human Burkitt's lymphoma cell line, Raji human Burkitt's lymphoma cell line, and RPMI 8226 human multiple myeloma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep ...read more
IRF4 was detected in immersion fixed paraffin-embedded sections of human tonsil using Sheep Anti-Human IRF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5525) at 3 µg/mL overnight at 4 °C. Before ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

IRF4 Antibody Summary

Immunogen
E. coli-derived recombinant human IRF4
Glu130-Glu451
Accession # Q15306
Specificity
Detects human IRF4 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) IRF3, rhIRF5, and rhIRF6 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
IRF4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF5525 in the following applications:

Publications
Read Publication using
AF5525 in the following applications:

  • ICC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IRF4 Antibody

  • interferon regulatory factor 4
  • IRF4
  • IRF-4
  • LSIRF
  • LSIRFMUM1
  • Lymphocyte-specific interferon regulatory factor
  • Multiple myeloma oncogene 1
  • MUM1
  • NF-EM5

Background

Interferon regulatory factor 4 (IRF4), also known as MUM1 and LSIRF, is a 51 kDa lymphocyte-restricted transcription factor. It is required for immunoglobulin class switching and terminal differentiation of B cells. IRF4 is overexpressed in multiple myeloma and cooperates with Myc in an autoregulatory loop. In T cells, IRF4 is required for the production of IL-4. IRF4 contains an N-terminal DNA binding domain that is homologous to that in other IRF proteins. Within the C-terminal domain (aa 130‑451), human IRF4 shares 90% aa sequence identity with mouse and rat IRF4. Alternate splicing may generate isoforms with N-terminal, C-terminal, or internal deletions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IRF4 Antibody (AF5525)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC.


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ICC
(1)
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Human
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Review for IRF4 Antibody (AF5525) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: IF.

Reviews using AF5525:
Filter by Applications
IF
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Immunofluorescence IRF4 AF5525
Enlarge
reviewed by:
Natalie Erdmann
IF Human 12/07/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedU266 (multiple myeloma), LP-1 (multiple myeloma), Daudi (Burkitt lymphoma) and HeLa (Cervical adenocarcinoma)
SpeciesHuman
LotZPD011510A

Blocking

Blocking Details10% donkey serum in PBS 1 hour at room temperature

Primary Anitbody

Dilution Ratio1/200 1ary antibody diluted in 10% donkey serum/PBS incubated O/N at 4oC.

Secondary Antibody

Secondary DescriptionDonkey F(ab")2 anti-sheep IgG (H+L) Cy3
Secondary Manufacturer Cat#Jackson #713-166-147
Secondary Concentration1/2000

Details

Detection NotesZeiss AxioImager, Cy3 filter cube for 200 ms exposure
Fixation DetailsImmersion-fixed in 4% paraformaldehyde 10 minutes, 0.5% Triton X-100 5 minutes permeabilization at room temperature
Wash Description3X 5 minutes PBS after each step

Comments

CommentsPositive controls U266 and LP-1, negative/low controls Daudi and HeLa

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for IRF4 Antibody (AF5525) (0)

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Secondary Antibodies

 

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Bioinformatics Tool for IRF4 Antibody (AF5525)

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Natalie Erdmann
12/07/2015
Application: IF
Species: Human

Bioinformatics

Gene Symbol IRF4
Entrez
Uniprot