BLIMP1/PRDM1 Antibody (3H2-E8)

Images

 
Western Blot: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Detection of Blimp-1 in murine plasmacytoma cell lysate (P3X) using NB 600-235. 1881: murine pre-B cell lysate (negative control). Photo courtesy of DA ...read more
Immunocytochemistry/ Immunofluorescence: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Double IF for Blimp-1 (green) and Ki-67 (proliferation, red) with DAPI counterstain, at 10x magnification (scale bar is 10um). ...read more
Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Analysis using the Biotin conjugate of NB600-235. Staining of Blimp-1 in mouse spleen. Image courtesy of product review by Branislav Krljanac.
Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - IL-10+Blimp-1-, IL-10+Blimp-1+, IL-10-Blimp-1+ and IL-10-Blimp-1- cells, % total living single splenic CD19+ cells of 3 total mice (Tedder Lab; Duke Univ).
Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Blimp-1 expression by IL-10+ (black line), IL-10- (dotted line) or CD8+ (thin, shaded line) cells (Tedder Lab; Duke Univ)
Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Blimp-1 expression by CD19+ (thick black line) or CD8+ (thin, shaded line). (Tedder Lab; Duke Univ).

Product Details

Summary
Reactivity Hu, Mu, PoSpecies Glossary
Applications WB, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
3H2-E8
Clonality
Monoclonal
Host
Mouse
Concentration
1 mg/ml

BLIMP1/PRDM1 Antibody (3H2-E8) Summary

Immunogen
A fragment of mouse Blimp-1 corresponding to residues 199-409. [UniProt# Q60636]
Localization
Nuclear
Marker
Plasma Cell Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PRDM1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Chromatin Immunoprecipitation 1ug antibody / 10 ug protein
  • ELISA 1:100-1:2000
  • Flow Cytometry 8 ug/ml where cells are used at 2*10^6/mL
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation
Application Notes
This Blimp-1 (3H2-E8) antibody is useful for ELISA, Immunocytochemistry/Immunofluorescence, Western Blot, Immunohistochemistry on paraffin-embedded sections, Flow Cytometry, Immunoprecipitation and Chromatin Immunoprecipitation applications. Immunohistochemistry-Frozen was reported in scientific literature. By WB, this antibody recognizes a band at ~98 kDa and may recognize one at ~80 kDa (the beta form). Antigen retrieval is recommended (EDTA buffer, microwave) prior to IHC on paraffin tissues. This antibody demonstrates nuclear staining. For IHC and ICC/IF a dilution of 1:200 is recommended with tyramide amplification.
Positive Control
BLIMP1/PRDM1 Lysate (NBL1-14733)
Reviewed Applications
Read 2 Reviews rated 3.5
using
NB600-235 in the following applications:

Publications
Read Publications using
NB600-235 in the following applications:

Reactivity Notes

Human, mouse and pig.

Alternate Names for BLIMP1/PRDM1 Antibody (3H2-E8)

  • Beta-interferon gene positive regulatory domain I-binding factor
  • beta-interferon gene positive-regulatory domain I binding factor
  • BLIMP1
  • BLIMP-1
  • BLIMP1MGC118925
  • blmp-1
  • B-lymphocyte-induced maturation protein 1
  • MGC118922
  • MGC118923
  • Positive regulatory domain I-binding factor 1
  • PR domain containing 1, with ZNF domain
  • PR domain zinc finger protein 1
  • PR domain-containing protein 1
  • PRDI-BF1MGC118924
  • PRDI-binding factor 1
  • PRDI-binding factor-1
  • PRDM1
  • PR-domain zinc finger protein 1
  • ZNFPR1A1

Background

Blimp-1 (B lymphocyte-induced maturation protein) is a nuclear zinc-finger containing transcriptional repressor which primarily controls the terminal differentiation of mature B cells to plasma cells, and also involves in macrophage as well as T-cells population homeostasis/differentiation. It is also called positive regulatory domain I-binding factor-1 (PRDI-BF1) or PR (PRDI-BF1-RIZ) domain zinc finger protein 1 (PRDM1) and its first human homolog, PRDI-BF1, was identified by its ability to bind to the PRDI element on the IFN-beta promoter leading to inhibition of virus-mediated IFN-beta production. Blimp-1 contains N-terminal PR/SET domain and five C2H2 zinc fingers located near its C-terminus that mediate DNA binding, nuclear import and recruitment of histone modifying enzymes, and these activities enable Blimp-1 for its ability to control cell-fate decisions in the embryo and govern tissue homeostasis in multiple cell types in the adult organism. Blimp-1 recruits chromatin-modifying enzymes including HDACs and methyltransferases, and some of its target genes include c-Myc, CIITA, Pax5, Spi-B, and Id3. Blimp-1 sumoylation at Lys-816 by PIAS1 augments transcriptional repressor activity, and is critical for plasma cell differentiation. Deletion mutation of Blimp-1 is frequently observed in several tumors including lymphoid malignancies and has been proposed to be a candidate of tumor suppressor gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Po
Applications: WB, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP

Publications for BLIMP1/PRDM1 Antibody (NB600-235)(40)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 7 applications: FLOW, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB.


Filter By Application
FLOW
(6)
ICC/IF
(4)
IHC
(1)
IHC-Fr
(1)
IHC-P
(7)
IP
(2)
WB
(15)
All Applications
Filter By Species
Human
(16)
Mouse
(12)
All Species
Showing Publications 1 - 10 of 40. Show All 40 Publications.
Publications using NB600-235 Applications Species
McCarthy C, Kenny LC. Therapeutically targeting mitochondrial redox signalling alleviates endothelial dysfunction in preeclampsia Sci Rep Sep 8 2016 12:00AM [PMID:27604418] (ICC/IF, Human) ICC/IF Human
Jang KJ, Mano H, Aoki K et al. Mitochondrial function provides instructive signals for activation-induced B-cell fates Nat Commun 2015 Apr 10 [PMID:25857523] (WB, FLOW, Mouse) WB, FLOW Mouse
Ramon S, Baker Sf, Sahler Jm et al. The Specialized Proresolving Mediator 17-HDHA Enhances the Antibody-Mediated Immune Response against Influenza Virus: A new Class of Adjuvant? J. Immunol. 2014 Nov 12 [PMID:25392529] (WB) WB
Gosal K, Dunlop K, Dhaliwal R et al. Rho-kinase Mediates Right Ventricular Systolic Dysfunction in Rats with Chronic neonatal Pulmonary Hypertension. Am. J. Respir. Cell Mol. Biol. 2014 Oct 22 [PMID:25337652] (FLOW) FLOW
Joo H, Li D, Dullaers M et al. C-Type Lectin-like Receptor LOX-1 Promotes Dendritic Cell-Mediated Class-Switched B Cell Responses.

Details:
Using the PE conjugated version of NB600-235, catalog number NB600-235PE. Immunity 2014 Oct 08 [PMID:25308333] (FLOW, Human)
FLOW Human
Rasul E, Salamon D, Nagy N et al. The MEC1 and MEC2 Lines Represent Two CLL Subclones in Different Stages of Progression towards Prolymphocytic Leukemia. PLoS ONE. 2014 Aug 28 [PMID:25162594] (WB, Human) WB Human
Kim J, Nakasaki M, Todorova D et al. p53 induces skin aging by depleting Blimp1(+) sebaceous gland cells. Cell Death Dis 3/28/2014 [PMID:24675459] (IHC-P, ICC/IF, FLOW, Mouse) IHC-P, ICC/IF, FLOW Mouse
Horn M, Geisen C, Cermak L et al. DRE-1/FBXO11-Dependent Degradation of BLMP-1/BLIMP-1 Governs C. elegans Developmental Timing and Maturation. Dev. Cell 3/4/2014 [PMID:24613396] (WB, Human) WB Human
Lin MH, Yeh LT, Chen SJ et al. T cell-specific BLIMP-1 deficiency exacerbates experimental autoimmune encephalomyelitis in nonobese diabetic mice by increasing Th1 and Th17 cells. Clin Immunol. 2014 Mar 10 [PMID:24568746] (WB, Mouse) WB Mouse
Severa M, Islam SA, Waggoner SN et al. The Transcriptional Repressor BLIMP1 Curbs Host Defenses by Suppressing Expression of the Chemokine CCL8. J. Immunol. 2014 Feb 24 [PMID:24477914] (WB, Mouse) WB Mouse
Show All 40 Publications.

Reviews for BLIMP1/PRDM1 Antibody (NB600-235) (2) 3.52

Average Rating: 3.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Other.
We have 2 reviews tested in 2 application: IHC-P, IF.

Reviews using NB600-235:
Filter by Applications
IHC-P
(1)
IF
(1)
All Applications
Filter by Species
Other
(2)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IHC-P Other 05/31/2010
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedPorcine embryo
SpeciesOther
CommentsI stained paraffin embedded porcine embryonic tissue to verify the usefulness of Blimp1 as a germline marker. The staining was highly specific (nuclear), but only successful when using chromogenic development (used the HRP-based AEC-red system). Citrate buffer was superior to EDTA for antigen retrieval and 1:500 dilution in 0.05M Tris-HCl, pH 6.0 with 1% BSA was superior to higher concentrations and higher pH or PBS-based solution.  Detection Method: AEC red. Negative Control: no primary and isotype control. Deparaffinization: 2x10min xylene, 2x 5min 99% etOH, 3min 96% etOH, 3min 70% etOH, 3 min water. Antigen Retrieval: 0.01M citrate buffer, 15min at boiling.

Blocking

Blocking DetailsBlocking Buffer: 2% BSA, Blocking Time: 10 minutes, Blocking Temp: room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:500, Incubation Dilution Buffer: 0.05M Tris-HCL, pH 6.0, 1 % BSA, Incubation Time: 1hr, Temp: RT

Secondary Antibody

Secondary DescriptionSecondary Ab: DAKO LSAB+ kit (SA-HRP link)

Details

Detection NotesPlease see comments
Fixation DetailsPFA fixed and paraffin embedded, Deparaffinization: 2x 10min xylene, 2x 5min 99% etOH, 3min 96% etOH, 3min 70% etOH, 3min water

Comments

CommentsI stained paraffin embedded porcine embryonic tissue to verify the usefulness of Blimp1 as a germline marker. The staining was highly specific (nuclear), but only successful when using chromogenic development (used the HRP-based AEC-red system). Citrate buffer was superior to EDTA for antigen retrieval and 1:500 dilution in 0.05M Tris-HCl, pH 6.0 with 1% BSA was superior to higher concentrations and higher pH or PBS-based solution.  Detection Method: AEC red. Negative Control: no primary and isotype control. Deparaffinization: 2x10min xylene, 2x 5min 99% etOH, 3min 96% etOH, 3min 70% etOH, 3 min water. Antigen Retrieval: 0.01M citrate buffer, 15min at boiling.
 
reviewed by:
Anonymous
IF Other 02/17/2009
View

Summary

ApplicationImmunofluorescence
Sample TestedBlimp1
SpeciesOther
LotSS

Blocking

Blocking DetailsBlocking Buffer: BSA, Blocking Time: 1 hour, Blocking Temp: 37 degrees Celsius

Primary Anitbody

Dilution RatioPrimary Ab Incubation Dilution Buffer: 0.5% BSA, Primary Ab Dilution Ratio: 1/200, Incubation Time:1.5 hr, Incubation Temp:37C

Secondary Antibody

Secondary DescriptionSecondary Ab: Alexa 594

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for BLIMP1/PRDM1 Antibody (NB600-235). (Showing 1 - 4 of 4 FAQs).

  1. Does your Blimp-1 Antibody (NB600-235) recognize both the alpha and beta form of BLIMP-1
    • By WB, this antibody recognizes a band ~98 kD and may recognize one ~80 kDa (the beta form).
  2. Do you have any images (dot plot chart or histogram) of human cells stained with this ab for flow cytometry?
    • Unfortunately, I do not have any additional images for this product on my records. This is one of our best selling products with great customer feedback and citations in at least 24 peer reviewed publications in journals of high repute. We stand by the quality of our products and all our products are 100% guaranteed. We are always there to help in case you face any trouble in generating your desired outcome out of the use of our products.
  3. Do you have any references which show this works in human for IF?
    • The research publication that has evidence for IF use of the above mentioned antibody in samples of human origin is: Julaton VT, Reijo Pera RA. NANOS3 function in human germ cell development. Hum Mol Genet. 2011 Jun 1;20(11):2238-50. Epub 2011 Mar 19.
  4. May we ask if NB600-235 is suitable for ChIP?
    • NB600-235 have been validated in IP, so since BLIMP-1 is a DNA binding protein, this antibody should be suitable for ChIP. If you would be interested in testing this novel application, please take a look at our Innovator's Reward program.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB600-235AF405
Alexa Fluor 488 Labeled NB600-235AF488
Alexa Fluor 647 Labeled NB600-235AF647
Alexa Fluor 700 Labeled NB600-235AF700
Allophycocyanin Labeled NB600-235APC
Biotin Labeled NB600-235B
DyLight 350 Labeled NB600-235UV
DyLight 405 Labeled NB600-235V
DyLight 488 Labeled NB600-235G
DyLight 550 Labeled NB600-235R
DyLight 650 Labeled NB600-235C
DyLight 680 Labeled NB600-235FR
DyLight 755 Labeled NB600-235IR
FITC Labeled NB600-235F
HRP Labeled NB600-235H
PE Labeled NB600-235PE
PerCP Labeled NB600-235PCP

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Contact Information

Product PDFs

Recent Reviews

3.5
5
0
4
1
3
1
2
0
1
0

 
Anonymous
05/31/2010
Application: IHC-P
Species: Other

 
Anonymous
02/17/2009
Application: IF
Species: Other

Bioinformatics

Gene Symbol PRDM1
Entrez
Uniprot

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