BLIMP1/PRDM1 Antibody (3H2-E8) - BSA Free

Western Blot: BLIMP1/PRDM1 Antibody (3H2-E8) - BSA Free [NB600-235] - BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - The effect of IL-21 and CD40L exposure on MEC1 and MEC2 cells. Expression of EBNA-2 and LMP-1 in IL-21 treated cells. Expression of EBNA-2, LMP-1 and Blimp-1 by immunoblotting; positive control: CBM1-Ral-STO, negative control: Ramos. 1.5x105 cells were loaded in the control lanes and 5x105 were loaded in both untreated and IL-21 treated MEC1 and MEC2 lanes. Note low expression of EBNA-2 and high expression of LMP-1 after IL-21 treatment and induction of Blimp-1 after IL-21 treatment. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0106008), licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-BLIMP1 (3H2-E8) at 10 ug/ml overnight at 4C and detected with an anti-mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Analysis using paraffin-embedded human tonsil tissue with BLIMP1/PRDM1 antibody at dilution of 1:50. Detection is completed through VC001 (DAB) and Counterstained by hematoxylin; labeling is predominantly in germinal centers. Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.

Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) - BSA Free [NB600-235] - An intracellular stain was performed on A431 cells with BLIMP1/PRDM1 Antibody (3H2-E8) NB600-235 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).

Western Blot: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Detection of Blimp-1 in murine plasmacytoma cell lysate (P3X) using NB 600-235. 1881: murine pre-B cell lysate (negative control). Photo courtesy of DA Savitsky, Columbia University.

Immunocytochemistry/Immunofluorescence: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Double IF for Blimp-1 (green) and Ki-67 (proliferation, red) with DAPI counterstain, at 10x magnification (scale bar is 10um). Positive surface epithelium and centrocytes are labelled. Photo courtesy of Dr. Giorgio Cattoretti, Institute for Cancer Genetics, Columbia University, NY.

Immunocytochemistry/Immunofluorescence: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-BLIMP1/PRDM1 (3H2-E8) conjugated to DyLight 550 [NB600-235R] at 20ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - IL-10+Blimp-1-, IL-10+Blimp-1+, IL-10-Blimp-1+ and IL-10-Blimp-1- cells, % total living single splenic CD19+ cells of 3 total mice (Tedder Lab; Duke Univ).

Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Blimp-1 expression by IL-10+ (black line), IL-10- (dotted line) or CD8+ (thin, shaded line) cells (Tedder Lab; Duke Univ)

Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Blimp-1 expression by CD19+ (thick black line) or CD8+ (thin, shaded line). (Tedder Lab; Duke Univ).

Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Analysis using the Biotin conjugate of NB600-235. Staining of Blimp-1 in mouse spleen. Image courtesy of product review by Branislav Krljanac.

Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - An intracellular stain was performed on U266 cells with BLIMP1/PRDM1 Antibody (3H2-E8) NB600-235 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and permeabilized with 0.1% Saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).

Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - An intracellular stain was performed on A431 cells with BLIMP1/PRDM1 [3H2-E8] Antibody NB600-235C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.

• Reactivity: Hu, Mu, Po
• Applications: WB, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
• Clone: 3H2-E8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

BLIMP1/PRDM1 Antibody (3H2-E8) - BSA Free Summary

• Immunogen: A fragment of mouse Blimp-1 corresponding to residues 199-409. [UniProt# Q60636]
• Localization: Nuclear
• Marker: Plasma Cell Marker
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PRDM1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation 1ug antibody / 10 ug protein
  • ELISA 1:100-1:2000
  • Flow Cytometry 8 ug/ml where cells are used at 2*10^6/mL
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation
  • Western Blot 1:1000
• Application Notes: This Blimp-1 (3H2-E8) antibody is useful for ELISA, Immunocytochemistry/Immunofluorescence, Western Blot, Immunohistochemistry on paraffin-embedded sections, Flow Cytometry, Immunoprecipitation and Chromatin Immunoprecipitation applications. Immunohistochemistry-Frozen was reported in scientific literature. By WB, this antibody recognizes a band at ~98 kDa and may recognize one at ~80 kDa (the beta form). Antigen retrieval is recommended (EDTA buffer, microwave) prior to IHC on paraffin tissues. This antibody demonstrates nuclear staining. For IHC and ICC/IF a dilution of 1:200 is recommended with tyramide amplification.
• Control:

  BLIMP1/PRDM1 Overexpression Lysate

Reactivity Notes

Porcine usage reported in customer reviews

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for BLIMP1/PRDM1 Antibody (3H2-E8) - BSA Free

• Beta-interferon gene positive regulatory domain I-binding factor
• beta-interferon gene positive-regulatory domain I binding factor
• BLIMP1
• BLIMP-1
• BLIMP1MGC118925
• blmp-1
• B-lymphocyte-induced maturation protein 1
• MGC118922
• MGC118923
• Positive regulatory domain I-binding factor 1
• PR domain containing 1, with ZNF domain
• PR domain zinc finger protein 1
• PR domain-containing protein 1
• PRDI-BF1MGC118924
• PRDI-binding factor 1
• PRDI-binding factor-1
• PRDM1
• PR-domain zinc finger protein 1
• ZNFPR1A1

Background

BLIMP-1 (B lymphocyte-induced maturation protein) is a nuclear zinc-finger containing transcriptional repressor which primarily controls the terminal differentiation of mature B cells to plasma cells. BLIMP-1 is also involved in macrophage and T-cell population homeostasis/differentiation. It is also called positive regulatory domain I-binding factor-1 (PRDI-BF1) or PR (PRDI-BF1-RIZ) domain zinc finger protein 1 (PRDM1) and its first human homolog, PRDI-BF1, was identified by its ability to bind to the PRDI element on the IFN-beta promoter leading to inhibition of virus-mediated IFN-beta production. BLIMP-1 contains N-terminal PR/SET domain and five C2H2 zinc fingers located near its C-terminus that mediate DNA binding, nuclear import and recruitment of histone modifying enzymes, and these activities enable BLIMP-1 to control cell-fate decisions in the embryo and govern tissue homeostasis in multiple cell types in the adult organism. BLIMP-1 recruits chromatin-modifying enzymes including HDACs and methyltransferases, and some of its target genes include c-Myc, CIITA, Pax5, Spi-B, and Id3. BLIMP-1 sumoylation at Lys-816 by PIAS1 augments transcriptional repressor activity, and is critical for plasma cell differentiation. Deletion mutation of BLIMP-1 is frequently observed in several tumor types including lymphoid malignancies, and has been proposed to be a candidate for tumor supression research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BLIMP1/PRDM1 Antibody (NB600-235)(51)

Publications using NB600-235

• Tan K, Wilkinson MF Regulation of both transcription and RNA turnover contribute to germline specification Nucleic acids research 2022-07-22 [PMID: 35776114] (ICC/IF)
  
  Details:
  Mouse ESCs were induced to differentiate into EpiLCs and PGCLCs
• Nishikawa K, Seno S, Yoshihara T Et al. Osteoclasts adapt to physioxia perturbation through DNA demethylation EMBO reports 2021-10-18 [PMID: 34661337] (WB, Mouse)
• Dong G, Li Y, Lee L et al. Genetic manipulation of primary human natural killer cells to investigate the functional and oncogenic roles of PRDM1 Haematologica 2020-07-30 [PMID: 32732362] (WB, Human)
• Wang Y, Wang C, Cai X et al. IL-21 Stimulates the expression and activation of cell cycle regulators and promotes cell proliferation in EBV-positive diffuse large B cell lymphoma Sci Rep 2020-07-23 [PMID: 32704112] (WB, Human)
• Iwanaga R, Truong BT, Lambert KA, et al. Loss of prdm1a accelerates melanoma onset and progression Mol Carcinog 2020-06-21 [PMID: 32562448]
• Hussaini M, Yeung C, Zhu X et al. PRDM1 expression levels in marginal zone lymphoma and lymphoplasmacytic lymphoma Int J Clin Exp Pathol 2020-01-23 [PMID: 31966717]
• Tian Y, Seumois G, De-Oliveira-Pinto LM Molecular Signatures of Dengue Virus-Specific IL-10/IFN-gamma Co-producing CD4 T Cells and Their Association with Dengue Disease Cell Rep 2019-12-24 [PMID: 31875555] (Human)
• Meer S, Perner Y, McAlpine E, Willem P Extra-oral plasmablastic lymphomas in a high HIV endemic area Histopathology Jul 30 2019 [PMID: 31361906] Germinality does not necessarily define mAb expression and thermal stability. Appl Microbiol Biotechnol. 2019-09-01 [PMID: 31361906]
  
  Details:
  Citation using the FITC version of this antibody.
• Ye, Y;Liu, M;Tang, L;Du, F;Liu, Y;Hao, P;Fu, Q;Guo, Q;Yan, Q;Zhang, X;Bao, C; Iguratimod represses B cell terminal differentiation linked with the inhibition of PKC/EGR1 axis Arthritis Res. Ther. 2019-04-11 [PMID: 30971291] (FLOW, Human)
• Cao, Y;Trillo-Tinoco, J;Sierra, RA;Anadon, C; ER stress-induced mediator C/EBP homologous protein thwarts effector Tcell activity in tumors through T-bet repression Nat Commun. [PMID: 30894532] (WB, Mouse)
• Wu L, Ehlin-Henriksson B, Zhou X et al. EBV provides survival factors to EBV+ DLBCL lines and modulates cytokine induced specific chemotaxis in EBV+ DLBCL Immunology 2017-07-12 [PMID: 28699226] (Human)
• McCarthy C, Kenny LC. Therapeutically targeting mitochondrial redox signalling alleviates endothelial dysfunction in preeclampsia Sci Rep 2016-09-08 [PMID: 27604418] (ICC/IF, Human)
• Jang KJ, Mano H, Aoki K et al. Mitochondrial function provides instructive signals for activation-induced B-cell fates Nat Commun. Nat Commun 2015-04-10 [PMID: 25857523] (WB, FLOW, Mouse)
  
  Details:
  Using the DyLight 650 conjugated version of NB600-302, catalog number NB600-302C.
• Ramon S, Baker Sf, Sahler Jm et al. The Specialized Proresolving Mediator 17-HDHA Enhances the Antibody-Mediated Immune Response against Influenza Virus: A new Class of Adjuvant? J. Immunol. 2014-11-12 [PMID: 25392529] (WB)
• Gosal K, Dunlop K, Dhaliwal R et al. Rho-kinase Mediates Right Ventricular Systolic Dysfunction in Rats with Chronic neonatal Pulmonary Hypertension. Am. J. Respir. Cell Mol. Biol. 2014-10-22 [PMID: 25337652] (FLOW)
• Joo H, Li D, Dullaers M et al. C-Type Lectin-like Receptor LOX-1 Promotes Dendritic Cell-Mediated Class-Switched B Cell Responses. Immunity 2014-10-08 [PMID: 25308333] (FLOW, Human)
  
  Details:
  Using the PE conjugated version of NB600-235, catalog number NB600-235PE.
• Rasul E, Salamon D, Nagy N et al. The MEC1 and MEC2 Lines Represent Two CLL Subclones in Different Stages of Progression towards Prolymphocytic Leukemia. PLoS ONE. 2014-08-28 [PMID: 25162594] (WB, Human)
• Kim J, Nakasaki M, Todorova D et al. p53 induces skin aging by depleting Blimp1(+) sebaceous gland cells. Cell Death Dis 2014-03-28 [PMID: 24675459] (ICC/IF, FLOW, IHC-P, Mouse)
• Horn M, Geisen C, Cermak L et al. DRE-1/FBXO11-Dependent Degradation of BLMP-1/BLIMP-1 Governs C. elegans Developmental Timing and Maturation. Dev. Cell 2014-03-04 [PMID: 24613396] (WB, Human)
• Lin MH, Yeh LT, Chen SJ et al. T cell-specific BLIMP-1 deficiency exacerbates experimental autoimmune encephalomyelitis in nonobese diabetic mice by increasing Th1 and Th17 cells. Clin Immunol. 2014-03-10 [PMID: 24568746] (WB, Mouse)
• Severa M, Islam SA, Waggoner SN et al. The Transcriptional Repressor BLIMP1 Curbs Host Defenses by Suppressing Expression of the Chemokine CCL8. J. Immunol. 2014-02-24 [PMID: 24477914] (WB, Mouse)
• Kim EH, Gasper DJ, Lee SH et al. Bach2 regulates homeostasis of Foxp3+ regulatory T cells and protects against fatal lung disease in mice. J. Immunol. 2014-02-01 [PMID: 24367030] (FLOW, Mouse)
• Wong LY, Brulois K, Toth Z et al. KSHV K4.2 immediate early gene product regulates immunoglobulin secretion and calcium homeostasis by interacting with and inhibiting pERP1. J Virol. 2013-08-28 [PMID: 23986581] (IHC-P, Human)
• Parlato S, Bruni R, Fragapane P et al. IFN-alpha Regulates Blimp-1 Expression via miR-23a and miR-125b in Both Monocytes-Derived DC and pDC. PLoS One. 2013-08-16 [PMID: 23977359] (WB, Human)
• Fan CC, Lee LY, Yu MY et al. Upregulated hPuf-A promotes breast cancer tumorigenesis. Tumour Biol. 2013-04-28 [PMID: 23625657]
• Lin MH, Chou FC, Yeh LT et al. B lymphocyte-induced maturation protein 1 (BLIMP-1) attenuates autoimmune diabetes in NOD mice by suppressing Th1 and Th17 cells Diabetologia 2012-10-07 [PMID: 23052053] (WB, Mouse)
• Lee SR, Rutan JA, Monteith AJ et al. Receptor Cross-Talk Spatially Restricts p-ERK during TLR4 Stimulation of Autoreactive B Cells J Immunol 2012-09-14 [PMID: 22984080] (WB, Mouse)
• Takata K, Sato Y, Nakamura N et al. Duodenal follicular lymphoma lacks AID but expresses BACH2 and has memory B-cell characteristics Mod Pathol 2012-08-17 [PMID: 22899287] (IF/IHC, Human)
• Morgan MA, Mould AW, Li L et al. Alternative splicing regulates prdm1/blimp-1 DNA binding activities and corepressor interactions Mol Cell Biol 2012-09-01 [PMID: 22733990] (IP, Human)
• Ramon S, Gao F, Serhan CN, Phipps RP. Specialized Proresolving Mediators Enhance Human B Cell Differentiation to Antibody-Secreting Cells J Immunol 2012-06-18 [PMID: 22711890] (WB, Human)
• Le Gallou S, Caron G, Delaloy C et al. IL-2 Requirement for Human Plasma Cell Generation: Coupling Differentiation and Proliferation by Enhancing MAPK-ERK Signaling J Immunol 2012-07-01 [PMID: 22634617] (WB, Human)
• Altin JA, Goodnow CC, Cook MC. IL-10+CTLA-4+ Th2 Inhibitory Cells Form in a Foxp3-Independent, IL-2-Dependent Manner from Th2 Effectors during Chronic Inflammation. J Immunol;188(11):5478-88. 2012-06-01 [PMID: 22547705] (FLOW, Mouse)
• Nagy N, Adori M, Rasul A et al. Soluble factors produced by activated CD4+ T cells modulate EBV latency Proc Natl Acad Sci U S A 2012-01-31 [PMID: 22307606] (WB, Human)
• Gao Y, Kazama H, Yonehara S. Bim regulates B-cell receptor-mediated apoptosis in the presence of CD40 signaling in CD40-pre-activated splenic B cells differentiating into plasma cells. Int Immunol. 2012-02-01 [PMID: 22301689]
• Maseda D, Smith SH, Dilillo DJ et al. Regulatory B10 Cells Differentiate into Antibody-Secreting Cells After Transient IL-10 Production In Vivo. Journal of Immunology 2011-12-23 [PMID: 22198952]
• Shimshon L, Michaeli A, Hadar R et al. SUMOylation of Blimp-1 promotes its proteasomal degradation. FEBS Lett;585(15):2405-9. 2011-08-04 [PMID: 21722636] (IP, Mouse)
• Julaton VTA, Reijo Pera RA. NANOS3 function in human germ cell development. Hum Mol Genet;20(11):2238-50. 2011-06-01 [PMID: 21421998] (ICC/IF, Human)
• Matsushita T, Tedder TF. Identifying regulatory B cells (B10 cells) that produce IL-10 in mice. Methods Mol Biol;677:99-111. 2011-01-01 [PMID: 20941605]
• Boy S, van Heerden M, Pool R, Willem P. Plasmablastic lymphoma versus diffuse large B cell lymphoma with plasmablastic differentiation: proposal for a novel diagnostic scoring system Journal of Hematopathology. 2015-01-15 (IHC-P, Human)
  
  Details:
  BLIMP1/PRDM1 antibody used for IHC-P staining on CD20-negative, ALK-negative, HHV8-negative non-Hodgkin?s human lymphomas with plasmablastic differentiation defined by their morphological features, high proliferation index and positivity for MUM1/IRF4 and PRDM/Blimp1 protein expression. IHC-P assay involved - Heat-induced epitope retrieval with TRIS/EDTA pH9 buffer, quenching of endogenous peroxidase activity with H2O2, 1:200 dilution of primary antibody, detection with Novolink Polymer Detection Kit which followed counterstaining with haematoxylin (Fig 3).
• Nishikawa K, Nakashima T, Hayashi M et al. Blimp1-mediated repression of negative regulators is required for osteoclast differentiation. Proc Natl Acad Sci U S A 2010-02-16 [PMID: 20133620]
• Brzezinski JA 4th, Lamba DA, Reh TA. Blimp1 controls photoreceptor versus bipolar cell fate choice during retinal development. Development 2010-02-01 [PMID: 20110327]
• Garcia-Bates TM, Baglole CJ, Bernard MP et al. Peroxisome proliferator-activated receptor gamma ligands enhance human B cell antibody production and differentiation. J Immunol;183(11):6903-12. 2009-12-01 [PMID: 19915048]
• Wang X, Belguise K, O'Neill CF et al. RelB NF-kappaB represses estrogen receptor alpha expression via induction of the zinc finger protein Blimp1. Mol Cell Biol 2008-05-01 [PMID: 19433448]
• Courts C, Montesinos-Rongen M, Brunn A, Bug S, Siemer D, Hans V, Blumcke I, Klapper W, Schaller C, Wiestler OD, Kuppers R, Siebert R, Deckert M. Recurrent inactivation of the PRDM1 gene in primary central nervous system lymphoma. J Neuropathol Exp Neurol;67(7):720-7. 2008-07-01 [PMID: 18596541] (IHC-Fr, IHC-P, Human)
• Robertson EJ, Charatsi I, Joyner CJ et al. Blimp1 regulates development of the posterior forelimb, caudal pharyngeal arches, heart and sensory vibrissae in mice. Development;134(24):4335-45. 2007-12-01 [PMID: 18039967] (ICC/IF, Mouse)
• John, S A et al. Ets-1 regulates plasma cell differentiation by interfering with the activity of the transcription factor Blimp-1. J Biol Chem;283(2):951-62. 2008-01-11 [PMID: 17977828] (WB, Mouse)
• Liu, Y et al. Rituximab plus CHOP (R-CHOP) overcomes PRDM1-associated resistance to chemotherapy in patients with diffuse large B-cell lymphoma. Blood;110(1):339-44. 2007-07-01 [PMID: 17379744] (WB, IHC-P, Human)
• Gong, D, Malek, T R. Cytokine-dependent Blimp-1 expression in activated T cells inhibits IL-2 production. J Immunol;178(1):242-52. 2007-01-01 [PMID: 17182561]
• Severa, M et al. Toll-like receptor-dependent and -independent viperin gene expression and counter-regulation by PRDI-binding factor-1/BLIMP1. J Biol Chem;281(36):26188-95. 2006-09-08 [PMID: 16849320]
• Cattoretti, G et al. PRDM1/Blimp-1 is expressed in human B-lymphocytes committed to the plasma cell lineage. J Pathol;206(1):76-86. 2005-05-01 [PMID: 15772984] (IHC-P, WB, Human)
• Chang, DH et al. The dynamic expression pattern of B lymphocyte induced maturation protein-1 (Blimp-1) during mouse embryonic development. Mech Dev 1179(1-2): 305-309. 2002-01-01 [PMID: 12204275] (IHC-P, Mouse)

Reviews for BLIMP1/PRDM1 Antibody (NB600-235) (2)

Average Rating: 3.5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Other.

reviewed by: Anne Friis Petersen IHC-P Other 05/31/2010

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Porcine embryo
• Species: Other

reviewed by: System Admin IF Other 02/17/2009

Summary

• Application: Immunofluorescence
• Sample Tested: Blimp1
• Species: Other
• Lot: SS

FAQs for BLIMP1/PRDM1 Antibody (NB600-235). (Showing 1 - 4 of 4 FAQs).

1. Does your Blimp-1 Antibody (NB600-235) recognize both the alpha and beta form of BLIMP-1
   • By WB, this antibody recognizes a band ~98 kD and may recognize one ~80 kDa (the beta form).
2. Do you have any images (dot plot chart or histogram) of human cells stained with this ab for flow cytometry?
   • Unfortunately, I do not have any additional images for this product on my records. This is one of our best selling products with great customer feedback and citations in at least 24 peer reviewed publications in journals of high repute. We stand by the quality of our products and all our products are 100% guaranteed. We are always there to help in case you face any trouble in generating your desired outcome out of the use of our products.
3. Do you have any references which show this works in human for IF?
   • The research publication that has evidence for IF use of the above mentioned antibody in samples of human origin is: Julaton VT, Reijo Pera RA. NANOS3 function in human germ cell development. Hum Mol Genet. 2011 Jun 1;20(11):2238-50. Epub 2011 Mar 19.
4. May we ask if NB600-235 is suitable for ChIP?
   • NB600-235 have been validated in IP, so since BLIMP-1 is a DNA binding protein, this antibody should be suitable for ChIP. If you would be interested in testing this novel application, please take a look at our Innovator's Reward program.

Bioinformatics

• Gene Symbol: PRDM1