BLIMP1/PRDM1 Antibody (3H2-E8) - BSA Free

Images

 
Biological Strategies: Western Blot: BLIMP1/PRDM1 Antibody (3H2-E8) - BSA Free [NB600-235] - BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - The effect of IL-21 and CD40L exposure on MEC1 and MEC2 cells. Expression ...read more
Immunocytochemistry/ Immunofluorescence: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The ...read more
Immunohistochemistry-Paraffin: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Analysis using paraffin-embedded human tonsil tissue with BLIMP1/PRDM1 antibody at dilution of 1:50. Detection is completed through VC001 (DAB) ...read more
Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) - BSA Free [NB600-235] - An intracellular stain was performed on A431 cells with BLIMP1/PRDM1 Antibody (3H2-E8) NB600-235 (blue) and a matched isotype control MAB002 ...read more
Western Blot: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Detection of Blimp-1 in murine plasmacytoma cell lysate (P3X) using NB 600-235. 1881: murine pre-B cell lysate (negative control). Photo courtesy of DA ...read more
Immunocytochemistry/ Immunofluorescence: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Double IF for Blimp-1 (green) and Ki-67 (proliferation, red) with DAPI counterstain, at 10x magnification (scale bar is 10um). ...read more
Immunocytochemistry/ Immunofluorescence: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The ...read more
Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - IL-10+Blimp-1-, IL-10+Blimp-1+, IL-10-Blimp-1+ and IL-10-Blimp-1- cells, % total living single splenic CD19+ cells of 3 total mice (Tedder Lab; Duke Univ).
Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Blimp-1 expression by IL-10+ (black line), IL-10- (dotted line) or CD8+ (thin, shaded line) cells (Tedder Lab; Duke Univ)
Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Blimp-1 expression by CD19+ (thick black line) or CD8+ (thin, shaded line). (Tedder Lab; Duke Univ).
Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Analysis using the Biotin conjugate of NB600-235. Staining of Blimp-1 in mouse spleen. Image courtesy of product review by Branislav Krljanac.
Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - An intracellular stain was performed on U266 cells with BLIMP1/PRDM1 Antibody (3H2-E8) NB600-235 (blue) and a matched isotype control (orange). Cells were ...read more
Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - An intracellular stain was performed on A431 cells with BLIMP1/PRDM1 [3H2-E8] Antibody NB600-235C (blue) and a matched isotype control (orange). Cells were ...read more
DenA deneddylase activity.(A) Recombinant human DEN1 and fungal DenA deneddylate a human CUL1-Nedd8 substrate in vitro. SDS-PAGE and subsequent western analysis show cleavage of the substrate (∼60 kDa) producing the ...read more
Histone induce BMECs pyroptosis is dependent on activations of caspase 1, caspase 3 and NLRP3. BMECs were incubated with stimulated histone for 16 h, and the activities of these proteins were determined by western ...read more

Product Details

Summary
Reactivity Hu, Mu, PoSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IP, ChIP
Clone
3H2-E8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

BLIMP1/PRDM1 Antibody (3H2-E8) - BSA Free Summary

Immunogen
A fragment of mouse Blimp-1 corresponding to residues 199-409. [UniProt# Q60636]
Localization
Nuclear
Marker
Plasma Cell Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PRDM1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 1ug antibody/ 10 ug protein
  • ELISA 1:100-1:2000
  • Flow Cytometry 8 ug/ml where cells are used at 2*10^6/mL
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen reported in scientific literature
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation
  • Western Blot 1:1000
Application Notes
By WB, this antibody recognizes a band at ~98 kDa and may recognize one at ~80 kDa (the beta form). Antigen retrieval is recommended (EDTA buffer, microwave) prior to IHC on paraffin tissues. This antibody demonstrates nuclear staining. For IHC and ICC/IF a dilution of 1:200 is recommended with tyramide amplification.
Control
BLIMP1/PRDM1 Overexpression Lysate
Reviewed Applications
Read 2 Reviews rated 3.5
using
NB600-235 in the following applications:

Publications
Read Publications using
NB600-235 in the following applications:

Reactivity Notes

Porcine usage reported in customer reviews

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for BLIMP1/PRDM1 Antibody (3H2-E8) - BSA Free

  • Beta-interferon gene positive regulatory domain I-binding factor
  • beta-interferon gene positive-regulatory domain I binding factor
  • BLIMP1
  • BLIMP-1
  • BLIMP1MGC118925
  • blmp-1
  • B-lymphocyte-induced maturation protein 1
  • MGC118922
  • MGC118923
  • Positive regulatory domain I-binding factor 1
  • PR domain containing 1, with ZNF domain
  • PR domain zinc finger protein 1
  • PR domain-containing protein 1
  • PRDI-BF1MGC118924
  • PRDI-binding factor 1
  • PRDI-binding factor-1
  • PRDM1
  • PR-domain zinc finger protein 1
  • ZNFPR1A1

Background

BLIMP-1 (B lymphocyte-induced maturation protein) is a nuclear zinc-finger containing transcriptional repressor which primarily controls the terminal differentiation of mature B cells to plasma cells. BLIMP-1 is also involved in macrophage and T-cell population homeostasis/differentiation. It is also called positive regulatory domain I-binding factor-1 (PRDI-BF1) or PR (PRDI-BF1-RIZ) domain zinc finger protein 1 (PRDM1) and its first human homolog, PRDI-BF1, was identified by its ability to bind to the PRDI element on the IFN-beta promoter leading to inhibition of virus-mediated IFN-beta production. BLIMP-1 contains N-terminal PR/SET domain and five C2H2 zinc fingers located near its C-terminus that mediate DNA binding, nuclear import and recruitment of histone modifying enzymes, and these activities enable BLIMP-1 to control cell-fate decisions in the embryo and govern tissue homeostasis in multiple cell types in the adult organism. BLIMP-1 recruits chromatin-modifying enzymes including HDACs and methyltransferases, and some of its target genes include c-Myc, CIITA, Pax5, Spi-B, and Id3. BLIMP-1 sumoylation at Lys-816 by PIAS1 augments transcriptional repressor activity, and is critical for plasma cell differentiation. Deletion mutation of BLIMP-1 is frequently observed in several tumor types including lymphoid malignancies, and has been proposed to be a candidate for tumor supression research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BLIMP1/PRDM1 Antibody (NB600-235)(52)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 8 applications: B/N, FLOW, ICC/IF, IF/IHC, IHC-Fr, IHC-P, IP, WB.


Filter By Application
B/N
(1)
FLOW
(7)
ICC/IF
(5)
IF/IHC
(1)
IHC-Fr
(1)
IHC-P
(7)
IP
(2)
WB
(20)
All Applications
Filter By Species
Human
(21)
Mouse
(14)
All Species
Showing Publications 1 - 10 of 52. Show All 52 Publications.
Publications using NB600-235 Applications Species
Xiong E, Popp O, Salomon C et al. A CRISPR/Cas9-mediated screen identifies determinants of early plasma cell differentiation Frontiers in Immunology 2023-01-05 [PMID: 36685499] (B/N, WB) B/N, WB
Tan K, Wilkinson MF Regulation of both transcription and RNA turnover contribute to germline specification Nucleic acids research 2022-07-22 [PMID: 35776114] (ICC/IF)

Details:
Mouse ESCs were induced to differentiate into EpiLCs and PGCLCs
ICC/IF
Nishikawa K, Seno S, Yoshihara T Et al. Osteoclasts adapt to physioxia perturbation through DNA demethylation EMBO reports 2021-10-18 [PMID: 34661337] (WB, Mouse) WB Mouse
Dong G, Li Y, Lee L et al. Genetic manipulation of primary human natural killer cells to investigate the functional and oncogenic roles of PRDM1 Haematologica 2020-07-30 [PMID: 32732362] (WB, Human) WB Human
Wang Y, Wang C, Cai X et al. IL-21 Stimulates the expression and activation of cell cycle regulators and promotes cell proliferation in EBV-positive diffuse large B cell lymphoma Sci Rep 2020-07-23 [PMID: 32704112] (WB, Human) WB Human
Iwanaga R, Truong BT, Lambert KA, et al. Loss of prdm1a accelerates melanoma onset and progression Mol Carcinog 2020-06-21 [PMID: 32562448]
Hussaini M, Yeung C, Zhu X et al. PRDM1 expression levels in marginal zone lymphoma and lymphoplasmacytic lymphoma Int J Clin Exp Pathol 2020-01-23 [PMID: 31966717]
Tian Y, Seumois G, De-Oliveira-Pinto LM Molecular Signatures of Dengue Virus-Specific IL-10/IFN-gamma Co-producing CD4 T Cells and Their Association with Dengue Disease Cell Rep 2019-12-24 [PMID: 31875555] (Human) Human
Meer S, Perner Y, McAlpine E, Willem P Extra-oral plasmablastic lymphomas in a high HIV endemic area Histopathology Jul 30 2019 [PMID: 31361906] Germinality does not necessarily define mAb expression and thermal stability. Appl Microbiol Biotechnol. 2019-09-01 [PMID: 31361906]

Details:
Citation using the FITC version of this antibody.
Ye, Y;Liu, M;Tang, L;Du, F;Liu, Y;Hao, P;Fu, Q;Guo, Q;Yan, Q;Zhang, X;Bao, C; Iguratimod represses B cell terminal differentiation linked with the inhibition of PKC/EGR1 axis Arthritis Res. Ther. 2019-04-11 [PMID: 30971291] (FLOW, Human) FLOW Human
Show All 52 Publications.

Reviews for BLIMP1/PRDM1 Antibody (NB600-235) (2) 3.52

Average Rating: 3.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Other.

Reviews using NB600-235:
Filter by Applications
IHC-P
(1)
IF
(1)
All Applications
Filter by Species
Other
(2)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
IHC-P Other 05/31/2010
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedPorcine embryo
SpeciesOther
  3
reviewed by:
Verified Customer
IF Other 02/17/2009
View

Summary

ApplicationImmunofluorescence
Sample TestedBlimp1
SpeciesOther
LotSS

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for BLIMP1/PRDM1 Antibody (NB600-235). (Showing 1 - 4 of 4 FAQs).

  1. Does your Blimp-1 Antibody (NB600-235) recognize both the alpha and beta form of BLIMP-1
    • By WB, this antibody recognizes a band ~98 kD and may recognize one ~80 kDa (the beta form).
  2. Do you have any images (dot plot chart or histogram) of human cells stained with this ab for flow cytometry?
    • Unfortunately, I do not have any additional images for this product on my records. This is one of our best selling products with great customer feedback and citations in at least 24 peer reviewed publications in journals of high repute. We stand by the quality of our products and all our products are 100% guaranteed. We are always there to help in case you face any trouble in generating your desired outcome out of the use of our products.
  3. Do you have any references which show this works in human for IF?
    • The research publication that has evidence for IF use of the above mentioned antibody in samples of human origin is: Julaton VT, Reijo Pera RA. NANOS3 function in human germ cell development. Hum Mol Genet. 2011 Jun 1;20(11):2238-50. Epub 2011 Mar 19.
  4. May we ask if NB600-235 is suitable for ChIP?
    • NB600-235 have been validated in IP, so since BLIMP-1 is a DNA binding protein, this antibody should be suitable for ChIP. If you would be interested in testing this novel application, please take a look at our Innovator's Reward program.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional BLIMP1/PRDM1 Products

Research Areas for BLIMP1/PRDM1 Antibody (NB600-235)

Find related products by research area.

Blogs on BLIMP1/PRDM1.

Tired T cells: Hypoxia Drives T cell Exhaustion in the Tumor Microenvironment
By Hunter MartinezThe paradigm shifting view of the immune system being leveraged to target cancer has led to numerous therapeutic breakthroughs. One major cell group responsible for this revelation is a T cell. ...  Read full blog post.

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Recent Reviews

3.5
5
0
4
1
3
1
2
0
1
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Verified Customer
05/31/2010
Application: IHC-P
Species: Other

 
Verified Customer
02/17/2009
Application: IF
Species: Other

Bioinformatics

Gene Symbol PRDM1