BLIMP1/PRDM1 Antibody (3H2-E8) Summary
A fragment of mouse Blimp-1 corresponding to residues 199-409. [UniProt# Q60636]
Plasma Cell Marker
Protein G purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
- Western Blot 1:1000
- Chromatin Immunoprecipitation 1ug antibody / 10 ug protein
- ELISA 1:100-1:2000
- Flow Cytometry 8 ug/ml where cells are used at 2*10^6/mL
- Immunocytochemistry/Immunofluorescence 1:50-1:200
- Immunohistochemistry 1:200
- Immunohistochemistry-Paraffin 1:200
This Blimp-1 (3H2-E8) antibody is useful for ELISA, Immunocytochemistry/Immunofluorescence, Western Blot, Immunohistochemistry on paraffin-embedded sections, Flow Cytometry, Immunoprecipitation and Chromatin Immunoprecipitation applications. Immunohistochemistry-Frozen was reported in scientific literature. By WB, this antibody recognizes a band at ~98 kDa and may recognize one at ~80 kDa (the beta form). Antigen retrieval is recommended (EDTA buffer, microwave) prior to IHC on paraffin tissues. This antibody demonstrates nuclear staining. For IHC and ICC/IF a dilution of 1:200 is recommended with tyramide amplification.
|Read Publications using
NB600-235 in the following applications:
Porcine usage reported in customer reviews
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Protein G purified
Alternate Names for BLIMP1/PRDM1 Antibody (3H2-E8)
- Beta-interferon gene positive regulatory domain I-binding factor
- beta-interferon gene positive-regulatory domain I binding factor
- B-lymphocyte-induced maturation protein 1
- Positive regulatory domain I-binding factor 1
- PR domain containing 1, with ZNF domain
- PR domain zinc finger protein 1
- PR domain-containing protein 1
- PRDI-binding factor 1
- PRDI-binding factor-1
- PR-domain zinc finger protein 1
BLIMP-1 (B lymphocyte-induced maturation protein) is a nuclear zinc-finger containing transcriptional repressor which primarily controls the terminal differentiation of mature B cells to plasma cells. BLIMP-1 is also involved in macrophage and T-cell population homeostasis/differentiation. It is also called positive regulatory domain I-binding factor-1 (PRDI-BF1) or PR (PRDI-BF1-RIZ) domain zinc finger protein 1 (PRDM1) and its first human homolog, PRDI-BF1, was identified by its ability to bind to the PRDI element on the IFN-beta promoter leading to inhibition of virus-mediated IFN-beta production. BLIMP-1 contains N-terminal PR/SET domain and five C2H2 zinc fingers located near its C-terminus that mediate DNA binding, nuclear import and recruitment of histone modifying enzymes, and these activities enable BLIMP-1 to control cell-fate decisions in the embryo and govern tissue homeostasis in multiple cell types in the adult organism. BLIMP-1 recruits chromatin-modifying enzymes including HDACs and methyltransferases, and some of its target genes include c-Myc, CIITA, Pax5, Spi-B, and Id3. BLIMP-1 sumoylation at Lys-816 by PIAS1 augments transcriptional repressor activity, and is critical for plasma cell differentiation. Deletion mutation of BLIMP-1 is frequently observed in several tumor types including lymphoid malignancies, and has been proposed to be a candidate for tumor supression research.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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Product General Protocols
View specific protocols for BLIMP1/PRDM1 Antibody (NB600-235):
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FAQs for BLIMP1/PRDM1 Antibody (NB600-235). (Showing 1 - 4 of 4 FAQs).
Does your Blimp-1 Antibody (NB600-235) recognize both the alpha and beta form of BLIMP-1
- By WB, this antibody recognizes a band ~98 kD and may recognize one ~80 kDa (the beta form).
Do you have any images (dot plot chart or histogram) of human cells stained with this ab for flow cytometry?
- Unfortunately, I do not have any additional images for this product on my records. This is one of our best selling products with great customer feedback and citations in at least 24 peer reviewed publications in journals of high repute. We stand by the quality of our products and all our products are 100% guaranteed. We are always there to help in case you face any trouble in generating your desired outcome out of the use of our products.
Do you have any references which show this works in human for IF?
- The research publication that has evidence for IF use of the above mentioned antibody in samples of human origin is: Julaton VT, Reijo Pera RA. NANOS3 function in human germ cell development. Hum Mol Genet. 2011 Jun 1;20(11):2238-50. Epub 2011 Mar 19.
May we ask if NB600-235 is suitable for ChIP?
- NB600-235 have been validated in IP, so since BLIMP-1 is a DNA binding protein, this antibody should be suitable for ChIP. If you would be interested in testing this novel application, please take a look at our Innovator's Reward program.
Other Available Formats
Additional BLIMP1/PRDM1 Products
Bioinformatics Tool for BLIMP1/PRDM1 Antibody (NB600-235)
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Blogs on BLIMP1/PRDM1