Beta Ig-h3/TGFBI Antibody - BSA Free

Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody [NBP1-88606] - Analysis in human placenta and pancreas tissues using NBP1-88606 antibody. Corresponding TGFBI RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody [NBP1-88606] - Staining of human gastrointestinal, pancreas, placenta and skin using Anti-Beta Ig-h3/TGFBI antibody NBP1-88606 (A) shows similar protein distribution across tissues to independent antibody NBP2-48534 (B).

Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody [NBP1-88606] - Staining of human placenta shows moderate to strong membranous positivity in stromal cells.

Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody [NBP1-88606] - Staining of human eye shows moderate extracellular positivity in cornea.

Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody [NBP1-88606] - Staining of human gastrointestinal shows moderate positivity in extracellular matrix of glandular cells.

Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody [NBP1-88606] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody [NBP1-88606] - Staining of human skin shows moderate positivity in extracellular matrix of dermis.

Simple Western: Beta Ig-h3/TGFBI Antibody [NBP1-88606] - Simple Western lane view shows a specific band for Beta Ig-h3/TGFBI in 0.2 mg/ml of U-251 MG lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation systems.

Simple Western: Beta Ig-h3/TGFBI Antibody [NBP1-88606] - Electropherogram image of the corresponding Simple Western lane view. Beta Ig-h3/TGFBI antibody was used at 1:25 dilution on U-251 MG lysate(s) respectively.

• Reactivity: Hu, Mu, Rt
• Applications: Simple Western, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Beta Ig-h3/TGFBI Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Beta Ig-h3/TGFBI Antibody - BSA Free (NBP1-88606) is a polyclonal antibody validated for use in IHC and Simple Western. Anti-Beta Ig-h3/TGFBI Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: DHHATNGVVHLIDKVISTITNNIQQIIEIEDTFETLRAAVAASGLNTMLEGNGQYTLLAPTNEAFEKIPSETLNRILGDPEALRDLLNNHILKSAMCAEAIVAGLSVETLEGTTLEVGCSGDMLTINGKAIISNKD
• Predicted Species: Mouse (93%), Rat (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TGFBI
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Simple Western 1:25
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. In Simple Western only 10-15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in U-251 MG lysates, separated by Size, antibody dilution of 1:25
• Control Peptide:

  Beta Ig-h3/TGFBI Protein (NBP1-88606PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Beta Ig-h3/TGFBI Antibody - BSA Free

• beta IGH3
• beta IG-H3
• BIGH3EBMD
• CDB1
• CDG2
• CDGG1transforming growth factor, beta-induced, 68kD
• CSD
• CSD1
• CSD2
• CSD3
• Kerato-epithelin
• LCD1
• RGD-CAP
• RGD-containing collagen-associated protein
• TGFBI
• TGFBIP
• transforming growth factor, beta-induced, 68kDa
• transforming growth factor-beta-induced protein ig-h3

Background

TGFBI encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion. Mutations in this gene are associated with multiple types of corneal dystrophy. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Beta Ig-h3/TGFBI Antibody (NBP1-88606)(3)

Publications using NBP1-88606

• Zhu J, Chen X, Liao Z et al. TGFBI protein high expression predicts poor prognosis in colorectal cancer patients. Int J Clin Exp Pathol 2015-01-01 [PMID: 25755764] (IF/IHC, Human)
• Kuot A, Hewitt AW, Griggs K et al. Association of TCF4 and CLU polymorphisms with Fuchs' endothelial dystrophy and implication of CLU and TGFBI proteins in the disease process. Eur J Hum Genet 2012-06-01 [PMID: 22234156]
• Kashyap MK, Harsha HC, Renuse S et al. SILAC-based quantitative proteomic approach to identify potential biomarkers from the esophageal squamous cell carcinoma secretome. Cancer Biol Ther 2010-10-01 [PMID: 20686364]

Bioinformatics

• Gene Symbol: TGFBI