Aurora A Antibody

Simple Western: Aurora A Antibody [NBP1-51843] - Simple Western lane view shows a specific band for Aurora A in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.

Immunocytochemistry/Immunofluorescence: Aurora A Antibody [NBP1-51843] - IF Confocal analysis of HeLa cells using Aurora A antibody (NBP1-51843, 1:10). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).

Western Blot: Aurora A Antibody [NBP1-51843] - WB detection of Aurora A in HeLa whole cell lysate.

Immunohistochemistry: Aurora A Antibody [NBP1-51843] - IHC staining of Aurora A in mouse brain.

• Reactivity: Hu, Mu
• Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.56 mg/ml

Aurora A Antibody Summary

• Immunogen: A genomic peptide made to an N-terminal region of the human Aurora A protein (within residues 50-200). [Swiss-Prot O14965]
• Localization: Centrosome, Spindle pole. Note: Detected at the neurite hillock in developing neurons. Localizes on centrosomes in interphase cells and at each spindle pole in mitosis.
• Marker: Mitosis Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AURKA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000-1:10000
  • Simple Western 1:200
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
• Application Notes: This AURKA antibody is useful for Immunohistochemistry and Western blot, where a band is seen ~45 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Control:

  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 0.1% BSA, and 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 0.56 mg/ml
• Purity: Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for Aurora A Antibody

• AIK
• AIKAurA
• ARK1
• ARK-1
• ARK1EC 2.7.11.1
• AURA
• AURKA
• Aurora A
• aurora kinase AhARK1
• aurora/IPL1-like kinase
• Aurora/IPL1-related kinase 1
• Aurora-related kinase 1
• Breast tumor-amplified kinase
• breast-tumor-amplified kinase
• BTAK
• BTAKAURORA2
• EC 2.7.11
• IPL1-related kinase
• serine/threonine kinase 6
• serine/threonine protein kinase 15
• Serine/threonine-protein kinase 15
• serine/threonine-protein kinase 6
• Serine/threonine-protein kinase aurora-A
• STK15
• STK15serine/threonine kinase 15
• STK6
• STK6MGC34538
• STK7

Background

Aurora A is a cytoplasmic serine/threonine kinase that is critical in transitioning cells through mitosis and meiosis. Aurka acts through phosphorylating several spindle and tubulin proteins. Without Aurka, axon and neuron formation are negatively impacted. When these critical cellular processes are disrupted, there is a potential for tumorgenesis, and thus Aurka is implicated in many cancers, particularly those of the blood. In studies of angiofibromas, both MDM1 and Aurka were amplified. Overall, cell proliferation seems proportional to the expression of Aurka.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Aurora A Antibody (NBP1-51843)(5)

Publications using NBP1-51843

• Drpic Danica, Almeida Ana C, Aguiar Paulo et al. Chromosome Segregation Is Biased by Kinetochore Size. Current Biology : Cb 2018 [PMID: 29706521] (WB, Human)
• Gasca J, Flores ML, Giraldez S et al. Loss of FBXW7 and accumulation of MCL1 and PLK1 promote paclitaxel resistance in breast cancer. Oncotarget. Jul 7 2016 [PMID: 27409838] (WB)
• Flores ML, Castilla C, Gasca J, Medina R. Loss of PKCdelta induces prostate cancer resistance to paclitaxel through activation of Wnt/beta-Catenin pathway and Mcl-1 accumulation. Molecular Cancer Therapeutics. 2016 Apr 13 [PMID: 27196755] (IHC, Human)
• Casorzo L, Dell'Aglio C, Sarotto I, Risio M. Aurora kinase A gene copy number is associated with the malignant transformation of colorectal adenomas but not with the serrated neoplasia progression Hum. Pathol. 2015 Mar 01 [PMID: 25596657] (IHC-P, Human)
• Honma K, Nakanishi R, Nakanoko T et al. Contribution of Aurora-A and -B expression to DNA aneuploidy in gastric cancers Surg Today 2013 Apr 10 [PMID: 23572383] (IHC-P, Human)

Review for Aurora A Antibody (NBP1-51843) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Anony- mous ICC Human 07/01/2014

Summary

• Application: Immunocytochemistry
• Species: Human
• Special Applications: IF Confocal analysis of HeLa cells using Aurora A antibody (NBP1-51843, 1:10). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
• File: View PDF

FAQs for Aurora A Antibody (NBP1-51843). (Showing 1 - 2 of 2 FAQs).

1. What are the better positive controls for Aurora A Antibody (NBP1-51843) in the immunohistochemistry reaction, dilution used and antigen retrieval?
   • The dilution we recommend for IHC is 1:50 and here is our protocol for Antigen Unmasking: Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. As far as positive control is concerned, Aurora A is highly expressed in testis, colon, ovarian, prostate, neuroblastoma, breast and cervical cancer, so that you may include one of these tissues as your positive controls.
2. Aurora A Antibody NBP1-51843 says it is compatible for IF at 1:10-1:2000. Do you have a more accurate concentration for paraffin embedded tissue? 5um sections. Thanks.
   • IF/ICC is our abbreviation for immunofluorescencestaining of cells. For IHC with paraffin-embedded tissue the recommended starting dilution is 1:50. Antigen retrieval with sodium citrate buffer (pH 6.0) is recommended for this antibody when performing IHC-P. Please accept my apologies for any confusion, and feel free to contact me should you have any further questions.

Bioinformatics

• Gene Symbol: AURKA
• Entrez:
  • 6790(Human)
  • 20878(Mouse)
• Uniprot:
  • O14965()
  • P97477(Mouse)