Aurora A Antibody

Images

 
Western Blot: Aurora A Antibody [NBP1-51843] - WB detection of Aurora A in HeLa whole cell lysate.
Immunocytochemistry/ Immunofluorescence: Aurora A Antibody [NBP1-51843] - IF Confocal analysis of HeLa cells using Aurora A antibody (NBP1-51843, 1:10). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as ...read more
Immunohistochemistry: Aurora A Antibody [NBP1-51843] - IHC staining of Aurora A in mouse brain.
Western Blot: Aurora A Antibody [NBP1-51843] - Western blot analysis of HepG2, HEK 293, and COS cell lysate using Aurora A antibody at 1:1000.
Simple Western: Aurora A Antibody [NBP1-51843] - Simple Western lane view shows a specific band for Aurora A in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.56 mg/ml

Order Details

Aurora A Antibody Summary

Immunogen
A genomic peptide made to an N-terminal region of the human Aurora A protein (within residues 50-200). [Swiss-Prot O14965]
Localization
Cytoplasm - cytoskeleton - centrosome. Cytoplasm - cytoskeleton - spindle pole. Note: Detected at the neurite hillock in developing neurons. Localizes on centrosomes in interphase cells and at each spindle pole in mitosis.
Marker
Mitosis Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AURKA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Simple Western 1:200
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
Application Notes
This AURKA antibody is useful for Immunohistochemistry and Western blot, where a band is seen ~45 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Positive Control
HeLa Lysate (NB800-PC1)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-51843 in the following applications:

Publications
Read Publications using
NBP1-51843 in the following applications:

  • IHC
    1 publication
  • 2 publications
  • WB
    1 publication

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 0.1% BSA, and 50% Glycerol
Preservative
0.05% Sodium Azide
Concentration
0.56 mg/ml
Purity
Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for Aurora A Antibody

  • AIK
  • AIKAurA
  • ARK1
  • ARK-1
  • ARK1EC 2.7.11.1
  • AURA
  • AURKA
  • Aurora A
  • aurora kinase AhARK1
  • aurora/IPL1-like kinase
  • Aurora/IPL1-related kinase 1
  • Aurora-related kinase 1
  • Breast tumor-amplified kinase
  • breast-tumor-amplified kinase
  • BTAK
  • BTAKAURORA2
  • EC 2.7.11
  • IPL1-related kinase
  • serine/threonine kinase 6
  • serine/threonine protein kinase 15
  • Serine/threonine-protein kinase 15
  • serine/threonine-protein kinase 6
  • Serine/threonine-protein kinase aurora-A
  • STK15
  • STK15serine/threonine kinase 15
  • STK6
  • STK6MGC34538
  • STK7

Background

Aurora A is a cytoplasmic serine/threonine kinase that is critical in transitioning cells through mitosis and meiosis. Aurka acts through phosphorylating several spindle and tubulin proteins. Without Aurka, axon and neuron formation are negatively impacted. When these critical cellular processes are disrupted, there is a potential for tumorgenesis, and thus Aurka is implicated in many cancers, particularly those of the blood. In studies of angiofibromas, both MDM1 and Aurka were amplified. Overall, cell proliferation seems proportional to the expression of Aurka.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Aurora A Antibody (NBP1-51843)(4)

Review for Aurora A Antibody (NBP1-51843) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: ICC.

Reviews using NBP1-51843:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry Aurora A NBP1-51843
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reviewed by:
Anony- mous
ICC Human 07/01/2014
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Summary

ApplicationImmunocytochemistry
SpeciesHuman
Special ApplicationsIF Confocal analysis of HeLa cells using Aurora A antibody (NBP1-51843, 1:10). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Aurora A Antibody (NBP1-51843). (Showing 1 - 2 of 2 FAQs).

  1. What are the better positive controls for Aurora A Antibody (NBP1-51843) in the immunohistochemistry reaction, dilution used and antigen retrieval?
    • The dilution we recommend for IHC is 1:50 and here is our protocol for Antigen Unmasking: Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. As far as positive control is concerned, Aurora A is highly expressed in testis, colon, ovarian, prostate, neuroblastoma, breast and cervical cancer, so that you may include one of these tissues as your positive controls.
  2. Aurora A Antibody NBP1-51843 says it is compatible for IF at 1:10-1:2000. Do you have a more accurate concentration for paraffin embedded tissue? 5um sections. Thanks.
    • IF/ICC is our abbreviation for immunofluorescencestaining of cells. For IHC with paraffin-embedded tissue the recommended starting dilution is 1:50. Antigen retrieval with sodium citrate buffer (pH 6.0) is recommended for this antibody when performing IHC-P. Please accept my apologies for any confusion, and feel free to contact me should you have any further questions.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Anony- mous
07/01/2014
Application: ICC
Species: Human

Bioinformatics

Gene Symbol AURKA
Entrez
Uniprot