Aurora A Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: Aurora A Antibody [NBP1-51843] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti- (NBP1-51843) at 2 ug/ml overnight at ...read more
Simple Western: Aurora A Antibody [NBP1-51843] - Simple Western lane view shows a specific band for Aurora A in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa ...read more
Immunocytochemistry/ Immunofluorescence: Aurora A Antibody [NBP1-51843] - IF Confocal analysis of HeLa cells using Aurora A antibody (NBP1-51843, 1:10). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as ...read more
Western Blot: Aurora A Antibody [NBP1-51843] - WB detection of Aurora A in HeLa whole cell lysate.
Immunohistochemistry: Aurora A Antibody [NBP1-51843] - IHC staining of Aurora A in mouse brain.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Aurora A Antibody Summary

Immunogen
A recombinant protein made to an N-terminal region of the human Aurora A protein (within residues 50-200). [Swiss-Prot O14965]
Localization
Centrosome, Spindle pole. Note: Detected at the neurite hillock in developing neurons. Localizes on centrosomes in interphase cells and at each spindle pole in mitosis.
Marker
Mitosis Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AURKA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 2 ug/ml
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Simple Western 1:200
  • Western Blot 1 - 2 ug/ml
Application Notes
This AURKA antibody is useful for Immunohistochemistry and Western blot, where a band is seen ~45 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control
HeLa Whole Cell Lysate
Reviewed Applications
Read 1 Review rated 5
using
NBP1-51843 in the following applications:

Publications
Read Publications using
NBP1-51843 in the following applications:

  • IHC
    1 publication
  • 2 publications
  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Aurora A Antibody

  • AIK
  • AIKAurA
  • ARK1
  • ARK-1
  • ARK1EC 2.7.11.1
  • AURA
  • AURKA
  • Aurora A
  • aurora kinase AhARK1
  • aurora/IPL1-like kinase
  • Aurora/IPL1-related kinase 1
  • Aurora-related kinase 1
  • Breast tumor-amplified kinase
  • breast-tumor-amplified kinase
  • BTAK
  • BTAKAURORA2
  • EC 2.7.11
  • IPL1-related kinase
  • serine/threonine kinase 6
  • serine/threonine protein kinase 15
  • Serine/threonine-protein kinase 15
  • serine/threonine-protein kinase 6
  • Serine/threonine-protein kinase aurora-A
  • STK15
  • STK15serine/threonine kinase 15
  • STK6
  • STK6MGC34538
  • STK7

Background

Aurora A is a cytoplasmic serine/threonine kinase that is critical in transitioning cells through mitosis and meiosis. Aurka acts through phosphorylating several spindle and tubulin proteins. Without Aurka, axon and neuron formation are negatively impacted. When these critical cellular processes are disrupted, there is a potential for tumorgenesis, and thus Aurka is implicated in many cancers, particularly those of the blood. In studies of angiofibromas, both MDM1 and Aurka were amplified. Overall, cell proliferation seems proportional to the expression of Aurka.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Aurora A Antibody (NBP1-51843)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: IHC, IHC-P, WB.


Filter By Application
IHC
(1)
IHC-P
(2)
WB
(2)
All Applications
Filter By Species
Human
(5)
All Species

Review for Aurora A Antibody (NBP1-51843) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-51843:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry Aurora A NBP1-51843
Enlarge
5
reviewed by:
Anony- mous
ICC Human 07/01/2014
View

Summary

ApplicationImmunocytochemistry
SpeciesHuman
Special ApplicationsIF Confocal analysis of HeLa cells using Aurora A antibody (NBP1-51843, 1:10). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
FileView PDF

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Aurora A Antibody (NBP1-51843). (Showing 1 - 2 of 2 FAQs).

  1. What are the better positive controls for Aurora A Antibody (NBP1-51843) in the immunohistochemistry reaction, dilution used and antigen retrieval?
    • The dilution we recommend for IHC is 1:50 and here is our protocol for Antigen Unmasking: Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. As far as positive control is concerned, Aurora A is highly expressed in testis, colon, ovarian, prostate, neuroblastoma, breast and cervical cancer, so that you may include one of these tissues as your positive controls.
  2. Aurora A Antibody NBP1-51843 says it is compatible for IF at 1:10-1:2000. Do you have a more accurate concentration for paraffin embedded tissue? 5um sections. Thanks.
    • IF/ICC is our abbreviation for immunofluorescencestaining of cells. For IHC with paraffin-embedded tissue the recommended starting dilution is 1:50. Antigen retrieval with sodium citrate buffer (pH 6.0) is recommended for this antibody when performing IHC-P. Please accept my apologies for any confusion, and feel free to contact me should you have any further questions.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-51843AF350
Alexa Fluor 405 NBP1-51843AF405
Alexa Fluor 488 NBP1-51843AF488
Alexa Fluor 532 NBP1-51843AF532
Alexa Fluor 594 NBP1-51843AF594
Alexa Fluor 647 NBP1-51843AF647
Alexa Fluor 700 NBP1-51843AF700
Alexa Fluor 750 NBP1-51843AF750
Biotin NBP1-51843B
DyLight 350 NBP1-51843UV
DyLight 405 NBP1-51843V
DyLight 488 NBP1-51843G
DyLight 550 NBP1-51843R
DyLight 594 NBP1-51843DL594
DyLight 650 NBP1-51843C
DyLight 680 NBP1-51843FR
DyLight 755 NBP1-51843IR
FITC NBP1-51843F
HRP NBP1-51843H
Janelia Fluor 549 NBP1-51843JF549
Janelia Fluor 646 NBP1-51843JF646

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
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Anony- mous
07/01/2014
Application: ICC
Species: Human

Bioinformatics

Gene Symbol AURKA
Entrez
Uniprot