Aurora A Antibody


Western Blot: Aurora A Antibody [NBP1-51843] - WB detection of Aurora A in HeLa whole cell lysate.
Immunocytochemistry/ Immunofluorescence: Aurora A Antibody [NBP1-51843] - IF Confocal analysis of HeLa cells using Aurora A antibody (NBP1-51843, 1:10). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as more
Immunohistochemistry: Aurora A Antibody [NBP1-51843] - IHC staining of Aurora A in mouse brain.
Western Blot: Aurora A Antibody [NBP1-51843] - Western blot analysis of HepG2, HEK 293, and COS cell lysate using Aurora A antibody at 1:1000.
Simple Western: Aurora A Antibody [NBP1-51843] - Simple Western lane view shows a specific band for Aurora A in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa more

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
0.56 mg/ml

Order Details

Aurora A Antibody Summary

A genomic peptide made to an N-terminal region of the human Aurora A protein (within residues 50-200). [Swiss-Prot O14965]
Cytoplasm - cytoskeleton - centrosome. Cytoplasm - cytoskeleton - spindle pole. Note: Detected at the neurite hillock in developing neurons. Localizes on centrosomes in interphase cells and at each spindle pole in mitosis.
Mitosis Marker
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS, 0.1% BSA, and 50% Glycerol
0.05% Sodium Azide
0.56 mg/ml
Immunogen affinity purified


  • Western Blot 1:1000-1:10000
  • Simple Western 1:200
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
Application Notes
This AURKA antibody is useful for Immunohistochemistry and Western blot, where a band is seen ~45 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 1 Review rated 5
NBP1-51843 in the following applications:

Read Publications using
NBP1-51843 in the following applications:

  • IHC
    1 publication
  • 2 publications
  • WB
    1 publication

Reactivity Notes

Human and mouse.


Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for Aurora A Antibody

  • AIK
  • AIKAurA
  • ARK1
  • ARK-1
  • ARK1EC
  • AURA
  • aurora kinase AhARK1
  • aurora/IPL1-like kinase
  • Aurora/IPL1-related kinase 1
  • Aurora-related kinase 1
  • Breast tumor-amplified kinase
  • breast-tumor-amplified kinase
  • BTAK
  • EC 2.7.11
  • IPL1-related kinase
  • serine/threonine kinase 6
  • serine/threonine protein kinase 15
  • Serine/threonine-protein kinase 15
  • serine/threonine-protein kinase 6
  • Serine/threonine-protein kinase aurora-A
  • STK15
  • STK15serine/threonine kinase 15
  • STK6
  • STK6MGC34538
  • STK7


Aurora A is a cytoplasmic serine/threonine kinase that is critical in transitioning cells through mitosis and meiosis. Aurka acts through phosphorylating several spindle and tubulin proteins. Without Aurka, axon and neuron formation are negatively impacted. When these critical cellular processes are disrupted, there is a potential for tumorgenesis, and thus Aurka is implicated in many cancers, particularly those of the blood. In studies of angiofibromas, both MDM1 and Aurka were amplified. Overall, cell proliferation seems proportional to the expression of Aurka.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Aurora A Antibody (NBP1-51843)(4)

Reviews for Aurora A Antibody (NBP1-51843) (1) 51

Average Rating: 5
(Based on 1 reviews)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: ICC.

Reviews using NBP1-51843:
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Images Ratings Applications Species Date Details
Immunocytochemistry Aurora A NBP1-51843
reviewed by:
ICC Human 07/01/2014


Special ApplicationsIF Confocal analysis of HeLa cells using Aurora A antibody (NBP1-51843, 1:10). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Aurora A Antibody (NBP1-51843). (Showing 1 - 2 of 2 FAQs).

  1. What are the better positive controls for Aurora A Antibody (NBP1-51843) in the immunohistochemistry reaction, dilution used and antigen retrieval?
    • The dilution we recommend for IHC is 1:50 and here is our protocol for Antigen Unmasking: Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. As far as positive control is concerned, Aurora A is highly expressed in testis, colon, ovarian, prostate, neuroblastoma, breast and cervical cancer, so that you may include one of these tissues as your positive controls.
  2. Aurora A Antibody NBP1-51843 says it is compatible for IF at 1:10-1:2000. Do you have a more accurate concentration for paraffin embedded tissue? 5um sections. Thanks.
    • IF/ICC is our abbreviation for immunofluorescencestaining of cells. For IHC with paraffin-embedded tissue the recommended starting dilution is 1:50. Antigen retrieval with sodium citrate buffer (pH 6.0) is recommended for this antibody when performing IHC-P. Please accept my apologies for any confusion, and feel free to contact me should you have any further questions.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Contact Information

Product PDFs


Gene Symbol AURKA

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