Exosome component 10 Antibody

Western Blot: Exosome component 10 Antibody [NBP1-32870] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EXOSC10 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: Exosome component 10 Antibody [NBP1-32870] - Paraffin-embedded Cal27 xenograft, using antibody at 1:100 dilution.

Western Blot: Exosome component 10 Antibody [NBP1-32870] - Sample: 1000 ug 293T whole cell lysate/extract A. 30 ug 293T whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgG, C. Immunoprecipitation of EXOSC 10 protein by 2. 5 ug of EXOSC 10 antibody 7. 5% SDS-PAGE gel.

Western Blot: Exosome component 10 Antibody [NBP1-32870] - Sample (30 ug of whole cell lysate) A: 293T 7.5% SDS PAGE, antibody diluted at 1:3000.

Western Blot: Exosome component 10 Antibody [NBP1-32870] - analysis of EXOSC10 in HeLa whole cell extracts treated with (1) si-control and (2) EXOSC10 siRNA using anti-EXOSC10 antibody. Image from verified customer review.

• Reactivity: Hu, Ch, Pm
• Applications: WB, IHC, IHC-P, IP, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Exosome component 10 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human EXOSC10. The exact sequence is proprietary.
• Localization: Cytoplasm, Nucleus, nucleolus
• Predicted Species: Primate (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EXOSC10
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Knockdown Validated
  • Western Blot 1:1000-1:10000
• Theoretical MW: 101 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken reactivity reported in scientific literature (PMID: 24842995). Chimpanzee (99%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for Exosome component 10 Antibody

• autoantigen PM-SCL
• EC 3.1.13.-
• exosome component 10
• P100 polymyositis-scleroderma overlap syndrome-associated autoantigen
• p2
• p3
• p4
• PM/Scl-100Autoantigen PM/Scl 2
• PM-Scl
• PMSCL2PMSCL
• polymyositis/scleroderma autoantigen 2 (100kD)
• Polymyositis/scleroderma autoantigen 2
• polymyositis/scleroderma autoantigen 2, 100kDa
• RRP6
• Rrp6p
• RRP6Polymyositis/scleroderma autoantigen 100 kDa

Background

Part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Component of the exosome 3'->5' exoribonuclease complex, a complex that degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions. Required for the 3'processing of the 7S pre-RNA to the mature 5.8S rRNA. Could be an exonuclease . Plays a role in replication-dependent histone mRNA degradation. Mediates the association of MTR4, C1D and MPP6 wth the exosome and this complex is required for the maturation of 5.8S rRNA. Required for nucleolar localization of C1D

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Exosome component 10 Antibody (NBP1-32870)(3)

Publications using NBP1-32870

• Ogami K, Richard P, Chen Y et al. An Mtr4/ZFC3H1 complex facilitates turnover of unstable nuclear RNAs to prevent their cytoplasmic transport and global translational repression. Gens & Dev. 2017 Jul 21 [PMID: 28733371]
• Hsin JP, Li W, Hoque M et al. RNAP II CTD tyrosine 1 performs diverse functions in vertebrate cells. Elife (Cambridge) 2014 Jun 04 [PMID: 24842995] (WB, Chicken)
  
  Details:
  Exosome Component 10 antibody used for WB on lysates of 26r (DT40 cells derived Rpb1 derivative containing a CTD with 26 YSPTSPS repeats) and 25F+Y cells (Rpb1-Y1F derivative in which only a single F, in the C terminal-most heptad, was changed back to Y). WB data shown in Supplement Figure 4.
• Richard P, Feng S, Manley JL. A SUMO-dependent interaction between Senataxin and the exosome, disrupted in the neurodegenerative disease AOA2, targets the exosome to sites of transcription-induced DNA damage. Genes Dev. 2013 Oct 15 [PMID: 24105744] (WB, Human)

Reviews for Exosome component 10 Antibody (NBP1-32870) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Koichi Ogami WB Human 05/05/2015

Summary

• Application: Western Blot
• Sample Tested: Hela whole cell lysate
• Species: Human
• Lot: 40044

reviewed by: Verified Customer IP Human 08/02/2012

Summary

• Application: Immunoprecipitation
• Sample Tested: HeLa, U87 whole cell extract and nuclear extract
• Species: Human
• Lot: 40044

Bioinformatics

• Gene Symbol: EXOSC10
• Entrez:
  • 5394(Human)
• Uniprot:
  • Q01780(Human)