Exosome component 10 Antibody

Images

 
Western Blot: Exosome component 10 Antibody [NBP1-32870] - analysis of EXOSC10 in HeLa whole cell extracts treated with (1) si-control and (2) EXOSC10 siRNA using anti-EXOSC10 antibody. Image from verified customer ...read more
Immunohistochemistry-Paraffin: Exosome component 10 Antibody [NBP1-32870] - Paraffin-embedded Cal27 xenograft, using antibody at 1:100 dilution.
Western Blot: Exosome component 10 Antibody [NBP1-32870] - Sample: 1000 ug 293T whole cell lysate/extract A. 30 ug 293T whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgG, C. Immunoprecipitation ...read more
Western Blot: Exosome component 10 Antibody [NBP1-32870] - Sample (30 ug of whole cell lysate) A: 293T 7.5% SDS PAGE, antibody diluted at 1:3000.

Product Details

Summary
Reactivity Hu, Ch, PmSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.65 mg/ml

Order Details

Exosome component 10 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human EXOSC10. The exact sequence is proprietary.
Localization
Cytoplasm, Nucleus, nucleolus
Predicted Species
Primate (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EXOSC10
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
101 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 5
using
NBP1-32870 in the following applications:

Publications
Read Publications using
NBP1-32870 in the following applications:

  • WB
    2 publications

Reactivity Notes

Chicken reactivity reported in scientific literature (PMID: 24842995). Chimpanzee (99%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.65 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Exosome component 10 Antibody

  • autoantigen PM-SCL
  • EC 3.1.13.-
  • exosome component 10
  • P100 polymyositis-scleroderma overlap syndrome-associated autoantigen
  • p2
  • p3
  • p4
  • PM/Scl-100Autoantigen PM/Scl 2
  • PM-Scl
  • PMSCL2PMSCL
  • polymyositis/scleroderma autoantigen 2 (100kD)
  • Polymyositis/scleroderma autoantigen 2
  • polymyositis/scleroderma autoantigen 2, 100kDa
  • RRP6
  • Rrp6p
  • RRP6Polymyositis/scleroderma autoantigen 100 kDa

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Exosome component 10 Antibody (NBP1-32870)(3)

Reviews for Exosome component 10 Antibody (NBP1-32870) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.
We have 2 reviews tested in 2 application: WB, IP.

Reviews using NBP1-32870:
Filter by Applications
WB
(1)
IP
(1)
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Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot Exosome component 10 NBP1-32870
Enlarge
reviewed by:
Anonymous
WB Human 05/05/2015
View

Summary

ApplicationWestern Blot
Sample TestedHela whole cell lysate
SpeciesHuman
Lot40044

Blocking

Blocking DetailsTBS, 5% skim milk, 1h, r.t.,

Details

Detection NotesECL, 10sec-1min exposure
Immunoprecipitation Exosome component 10 NBP1-32870
Enlarge
reviewed by:
Patricia Richard
IP Human 08/02/2012
View

Summary

ApplicationImmunoprecipitation
Sample TestedHeLa, U87 whole cell extract and nuclear extract
SpeciesHuman
Lot40044
CommentsThis antibody works also in Western Blot

Blocking

Blocking Details20 min in PBST, 5% milk

Primary Anitbody

Dilution Ratio1:1000 in PBST 5% milk O/N at 4 degrees

Secondary Antibody

Secondary Descriptionanti-Rabbit IgG
Secondary Manufacturer Cat#A6154, Sigma
Secondary Concentration1:10000

Details

Detection NotesECL, 1 min to 5 min
Immunoprecipitation Detailsprotein G sepharose, O/N at 4 degrees

Comments

CommentsThis antibody works also in Western Blot

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Exosome component 10 Antibody (NBP1-32870) (0)

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Secondary Antibodies

 

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Anonymous
05/05/2015
Application: WB
Species: Human

Patricia Richard
08/02/2012
Application: IP
Species: Human

Bioinformatics

Gene Symbol EXOSC10
Entrez
Uniprot