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Exosome component 10 Antibody

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 (2 Reviews)
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Western Blot: Exosome component 10 Antibody [NBP1-32870] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EXOSC10 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: Exosome component 10 Antibody [NBP1-32870] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EXOSC10 antibody ...read more
Immunohistochemistry-Paraffin: Exosome component 10 Antibody [NBP1-32870] - Paraffin-embedded Cal27 xenograft, using antibody at 1:100 dilution.
Western Blot: Exosome component 10 Antibody [NBP1-32870] - Sample: 1000 ug 293T whole cell lysate/extract A. 30 ug 293T whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgG, C. Immunoprecipitation ...read more
Western Blot: Exosome component 10 Antibody [NBP1-32870] - Sample (30 ug of whole cell lysate) A: 293T 7.5% SDS PAGE, antibody diluted at 1:3000.
Western Blot: Exosome component 10 Antibody [NBP1-32870] - analysis of EXOSC10 in HeLa whole cell extracts treated with (1) si-control and (2) EXOSC10 siRNA using anti-EXOSC10 antibody. Image from verified customer ...read more

Product Details

Summary
Reactivity Hu, Ch, PmSpecies Glossary
Applications WB, IHC, IHC-P, IP, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Exosome component 10 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human EXOSC10. The exact sequence is proprietary.
Localization
Cytoplasm, Nucleus, nucleolus
Predicted Species
Primate (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EXOSC10
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Knockdown Validated
  • Western Blot 1:1000-1:10000
Theoretical MW
101 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 5
using
NBP1-32870 in the following applications:
Publications
Read Publications using
NBP1-32870 in the following applications:

  • WB
    2 publications

Reactivity Notes

Chicken reactivity reported in scientific literature (PMID: 24842995). Chimpanzee (99%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for Exosome component 10 Antibody

  • autoantigen PM-SCL
  • EC 3.1.13.-
  • exosome component 10
  • P100 polymyositis-scleroderma overlap syndrome-associated autoantigen
  • p2
  • p3
  • p4
  • PM/Scl-100Autoantigen PM/Scl 2
  • PM-Scl
  • PMSCL2PMSCL
  • polymyositis/scleroderma autoantigen 2 (100kD)
  • Polymyositis/scleroderma autoantigen 2
  • polymyositis/scleroderma autoantigen 2, 100kDa
  • RRP6
  • Rrp6p
  • RRP6Polymyositis/scleroderma autoantigen 100 kDa

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: CD77 Synthase Antibody [NBP1-90824] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Negative control (vector only transfected HEK293T lysate)Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402588)
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Western Blot: NUCKS1 Antibody [NB100-74635] - Detection of Human NUCKS by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NUCKS antibody NB100-74635 used for IP at 3 mcg/mg lysate. NUCKS was also immunoprecipitated by rabbit anti-NUCKS antibody NB100-74634, which recognizes an upstream epitope. Detection: Chemiluminescence with an exposure time of 30 seconds
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Simple Western: Exosome Component 9 Antibody [NBP1-71702] - Simple Western lane view shows a specific band for Exosome Component 9 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
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Western Blot: ELK3 Antibody (1H3) [NBP2-01264] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ELK3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ELK3.
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(3 Reviews)
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Flow Cytometry: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Analysis of Hela cells with hnRNP C1+C2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
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Western Blot: RRP4 Antibody [NBP2-97759] - Anti-RRP4 rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lane C: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 33 kDa. Observed band size: 33 kDa
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Exosome component 10 Antibody (NBP1-32870)(3)

We have publications tested in 2 confirmed species: Human, Chicken.

We have publications tested in 1 application: WB.


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Reviews for Exosome component 10 Antibody (NBP1-32870) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NBP1-32870:
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(1)
IP
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Images Ratings Applications Species Date Details
Western Blot Exosome component 10 NBP1-32870
Enlarge 		5
reviewed by:
Verified Customer
 WB Human 05/05/2015
View

Summary

ApplicationWestern Blot
Sample TestedHela whole cell lysate
SpeciesHuman
Lot40044

Blocking

Blocking DetailsTBS, 5% skim milk, 1h, r.t.,

Details

Detection NotesECL, 10sec-1min exposure
Immunoprecipitation Exosome component 10 NBP1-32870
Enlarge 		5
reviewed by:
Patricia Richard
 IP Human 08/02/2012
View

Summary

ApplicationImmunoprecipitation
Sample TestedHeLa, U87 whole cell extract and nuclear extract
SpeciesHuman
Lot40044
CommentsThis antibody works also in Western Blot

Blocking

Blocking Details20 min in PBST, 5% milk

Primary Anitbody

Dilution Ratio1:1000 in PBST 5% milk O/N at 4 degrees

Secondary Antibody

Secondary Descriptionanti-Rabbit IgG
Secondary Manufacturer Cat#A6154, Sigma
Secondary Concentration1:10000

Details

Detection NotesECL, 1 min to 5 min
Immunoprecipitation Detailsprotein G sepharose, O/N at 4 degrees

Comments

CommentsThis antibody works also in Western Blot

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FAQs for Exosome component 10 Antibody (NBP1-32870) (0)

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Recent Reviews

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Western Blot Exosome component 10 NBP1-32870
Enlarge
 Verified Customer
05/05/2015
Application: WB
Species: Human
read this review
Immunoprecipitation Exosome component 10 NBP1-32870
Enlarge
 Patricia Richard
08/02/2012
Application: IP
Species: Human
read this review

Bioinformatics

Gene Symbol EXOSC10
Entrez
Uniprot