Exosome component 10 Antibody

Western Blot: Exosome component 10 Antibody [NBP1-32870] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EXOSC10 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: Exosome component 10 Antibody [NBP1-32870] - Paraffin-embedded Cal27 xenograft, using antibody at 1:100 dilution.

Western Blot: Exosome component 10 Antibody [NBP1-32870] - Sample: 1000 ug 293T whole cell lysate/extract A. 30 ug 293T whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgG, C. Immunoprecipitation of EXOSC 10 protein by 2. 5 ug of EXOSC 10 antibody 7. 5% SDS-PAGE gel.

Western Blot: Exosome component 10 Antibody [NBP1-32870] - Sample (30 ug of whole cell lysate) A: 293T 7.5% SDS PAGE, antibody diluted at 1:3000.

Western Blot: Exosome component 10 Antibody [NBP1-32870] - analysis of EXOSC10 in HeLa whole cell extracts treated with (1) si-control and (2) EXOSC10 siRNA using anti-EXOSC10 antibody. Image from verified customer review.

• Reactivity: Hu, Ch, Pm
• Applications: WB, IHC, IHC-P, IP, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Exosome component 10 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human EXOSC10. The exact sequence is proprietary.
• Localization: Cytoplasm, Nucleus, nucleolus
• Predicted Species: Primate (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EXOSC10
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000-1:10000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Knockdown Validated
• Theoretical MW: 101 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken reactivity reported in scientific literature (PMID: 24842995). Chimpanzee (99%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for Exosome component 10 Antibody

• autoantigen PM-SCL
• EC 3.1.13.-
• exosome component 10
• P100 polymyositis-scleroderma overlap syndrome-associated autoantigen
• p2
• p3
• p4
• PM/Scl-100Autoantigen PM/Scl 2
• PM-Scl
• PMSCL2PMSCL
• polymyositis/scleroderma autoantigen 2 (100kD)
• Polymyositis/scleroderma autoantigen 2
• polymyositis/scleroderma autoantigen 2, 100kDa
• RRP6
• Rrp6p
• RRP6Polymyositis/scleroderma autoantigen 100 kDa

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Exosome component 10 Antibody (NBP1-32870)(3)

Publications using NBP1-32870

• Ogami K, Richard P, Chen Y et al. An Mtr4/ZFC3H1 complex facilitates turnover of unstable nuclear RNAs to prevent their cytoplasmic transport and global translational repression. Gens & Dev. 2017 Jul 21 [PMID: 28733371]
• Hsin JP, Li W, Hoque M et al. RNAP II CTD tyrosine 1 performs diverse functions in vertebrate cells. Elife (Cambridge) 2014 Jun 04 [PMID: 24842995] (WB, Chicken)
  
  Details:
  Exosome Component 10 antibody used for WB on lysates of 26r (DT40 cells derived Rpb1 derivative containing a CTD with 26 YSPTSPS repeats) and 25F+Y cells (Rpb1-Y1F derivative in which only a single F, in the C terminal-most heptad, was changed back to Y). WB data shown in Supplement Figure 4.
• Richard P, Feng S, Manley JL. A SUMO-dependent interaction between Senataxin and the exosome, disrupted in the neurodegenerative disease AOA2, targets the exosome to sites of transcription-induced DNA damage. Genes Dev. 2013 Oct 15 [PMID: 24105744] (WB, Human)

Reviews for Exosome component 10 Antibody (NBP1-32870) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Anonymous WB Human 05/05/2015

Summary

• Application: Western Blot
• Sample Tested: Hela whole cell lysate
• Species: Human
• Lot: 40044

Blocking

• Blocking Details: TBS, 5% skim milk, 1h, r.t.,

Details

• Detection Notes: ECL, 10sec-1min exposure

reviewed by: Patricia Richard IP Human 08/02/2012

Summary

• Application: Immunoprecipitation
• Sample Tested: HeLa, U87 whole cell extract and nuclear extract
• Species: Human
• Lot: 40044
• Comments: This antibody works also in Western Blot

Blocking

• Blocking Details: 20 min in PBST, 5% milk

Primary Anitbody

• Dilution Ratio: 1:1000 in PBST 5% milk O/N at 4 degrees

Secondary Antibody

• Secondary Description: anti-Rabbit IgG
• Secondary Manufacturer Cat#: A6154, Sigma
• Secondary Concentration: 1:10000

Details

• Detection Notes: ECL, 1 min to 5 min
• Immunoprecipitation Details: protein G sepharose, O/N at 4 degrees

Comments

• Comments: This antibody works also in Western Blot

Bioinformatics

• Gene Symbol: EXOSC10
• Entrez:
  • 5394(Human)
• Uniprot:
  • Q01780(Human)