TROP-2 Antibody

Images

 
Western blot shows lysates of NCI‑N87 human gastric carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TROP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF650) followed by ...read more
TROP‑2 was detected in immersion fixed paraffin-embedded sections of human brain (frontal cortex) using Goat Anti-Human TROP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF650) at 10 µg/mL overnight ...read more
PC‑3 human prostate cancer cell line was stained with Goat Anti-Human TROP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF650, filled histogram) or control antibody (Catalog # AB-108-C, open ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

TROP-2 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human TROP-2
Thr88-Thr274
Accession # P09758
Specificity
Detects human TROP-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) MCAM, rhNCAM‑L1, and rhBCAM is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TACSTD2
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • CyTOF-ready
Reviewed Applications
Read 1 Review rated 4
using
AF650 in the following applications:

Publications
Read Publications using
AF650 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TROP-2 Antibody

  • Cell surface glycoprotein Trop-2
  • EGP1
  • EGP-1
  • epithelial glycoprotein-1
  • GA733-1
  • GA733-1GP50
  • gastrointestinal tumor-associated antigen GA7331
  • gp50
  • M1S1cell surface glycoprotein TROP2
  • Membrane component chromosome 1 surface marker 1
  • membrane component, chromosome 1, surface marker 1
  • Pancreatic carcinoma marker protein GA733-1
  • pancreatic carcinoma marker protein GA7331,40kD glycoprotein, identified by monoclonal GA733
  • T16
  • TACSTD2
  • TROP2
  • TROP-2
  • TROP2GA7331
  • tumor-associated calcium signal transducer 2

Background

Human TROP-2, also called tumor associated calcium signal transducer 2 (TACSTD2), GA733-1, gp50 and T16, is a type I cell surface glycoprotein that is highly expressed on human carcinomas. It was originally identified as an antigen present on human gastrointestinal tumors and is the second of two members of this family. The other family member is GA733-2, also called EpCAM, TROP-1, 17-1A, gp40 and KSA. The TROP-2 gene is unique in that it contains no introns. A study of these two genes suggested that TROP-2 was the result of a retroposition of the EpCAM gene. TROP-2 and EpCAM share approximately 49% amino acid identity and approximately 67% similarity. Human and mouse TROP-2 share 87% similarity. The human TROP-2 protein consists of a putative 26 amino acid (aa) signal sequence, a 248 aa extracellular domain, a 23 aa transmembrane region and a 26 aa cytoplasmic domain. TROP-2 is capable of transducing an intracellular calcium signal and may play a role in tumor growth. It also has adhesive functions.

  1. Linnenbach, A.J. et al. (1989) Proc. Natl. Acad. Sci. USA 86:27.
  2. Linnenbach, A.J. et al. (1993) Mol. Cell. Biol. 13:1507.
  3. Fornaro, M. et al. (1995) Int. J. Cancer 62:610.
  4. Ripani, E. et al. (1998) Int. J. Cancer 76:671.
  5. El Sewedy, T. et al. (1998) Int. J. Cancer 75:324.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TROP-2 Antibody (AF650)(16)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 7 applications: Flow Cytometry, ICC, IHC, IHC-P, Immunoprecipitation, Neutralization, Western Blot.


Filter By Application
Flow Cytometry
(2)
ICC
(1)
IHC
(7)
IHC-P
(2)
Immunoprecipitation
(1)
Neutralization
(1)
Western Blot
(2)
All Applications
Filter By Species
Human
(15)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using AF650 Applications Species
Y Hao, D Zhang, Y Guo, Z Fu, D Yu, G Guan miR-488-3p sponged by circ-0000495 and mediated upregulation of TROP2 in head and neck squamous cell carcinoma development J Cancer, 2020;11(11):3375-3386. 2020 [PMID: 32231744] (IHC, Human) IHC Human
B Zeybek, A Manzano, A Bianchi, E Bonazzoli, S Bellone, N Buza, P Hui, S Lopez, E Perrone, P Manara, L Zammataro, G Altwerger, C Han, J Tymon-Rosa, G Menderes, E Ratner, DA Silasi, GS Huang, M Azodi, PE Schwartz, A Santin Cervical carcinomas that overexpress human trophoblast cell-surface marker (Trop-2) are highly sensitive to the antibody-drug conjugate sacituzumab govitecan Sci Rep, 2020;10(1):973. 2020 [PMID: 31969666] (IHC, Human) IHC Human
X Kuai, L Jia, T Yang, X Huang, W Zhao, M Zhang, Y Chen, J Zhu, Z Feng, Q Tang Trop2 Promotes Multidrug Resistance by Regulating Notch1 Signaling Pathway in Gastric Cancer Cells Med. Sci. Monit., 2020;26(0):e919566. 2020 [PMID: 31964857] (Western Blot, Human) Western Blot Human
C Han, E Perrone, B Zeybek, S Bellone, J Tymon-Rosa, G Altwerger, G Menderes, J Feinberg, K Haines, ME Muller Kar, A Bianchi, L Zammataro, A Manzano, E Bonazzoli, P Manara, N Buza, P Hui, E Ratner, DA Silasi, GS Huang, M Azodi, PE Schwartz, S Lopez, AD Santin In�vitro and in�vivo activity of sacituzumab govitecan, an antibody-drug conjugate targeting trophoblast cell-surface antigen 2 (Trop-2) in uterine serous carcinoma Gynecol. Oncol., 2019;0(0):. 2019 [PMID: 31839338] (IHC, Human) IHC Human
STK Sin, Y Li, M Liu, S Ma, XY Guan TROP-2 exhibits tumor suppressive functions in cervical cancer by dual inhibition of IGF-1R and ALK signaling Gynecol. Oncol., 2018;0(0):. 2018 [PMID: 30429055] (Western Blot, Human) Western Blot Human
S Vranic, J Palazzo, S Sanati, E Florento, E Contreras, J Xiu, J Swensen, Z Gatalica Potential Novel Therapy Targets in Neuroendocrine Carcinomas of the Breast Clin. Breast Cancer, 2018;0(0):. 2018 [PMID: 30268765] (IHC, Human) IHC Human
SM Zimmers, EP Browne, KE Williams, RM Jawale, CN Otis, SS Schneider, KF Arcaro TROP2 methylation and expression in tamoxifen-resistant breast cancer Cancer Cell Int., 2018;18(0):94. 2018 [PMID: 30002602] (IHC, Human) IHC Human
H Guan, Z Guo, W Liang, H Li, G Wei, L Xu, H Xiao, Y Li Trop2 enhances invasion of thyroid cancer by inducing MMP2 through ERK and JNK pathways BMC Cancer, 2017;17(1):486. 2017 [PMID: 28709407] (IHC, Human) IHC Human
Starodub A, Ocean A, Shah M, Guarino M, Picozzi V, Vahdat L, Thomas S, Govindan S, Maliakal P, Wegener W, Hamburger S, Sharkey R, Goldenberg D First-in-Human Trial of a Novel Anti-Trop-2 Antibody-SN-38 Conjugate, Sacituzumab Govitecan, for the Treatment of Diverse Metastatic Solid Tumors. Clin Cancer Res, 2015;21(17):3870-8. 2015 [PMID: 25944802] (IHC, Human) IHC Human
Xia Y, Li B, Gao N, Xia H, Men Y, Liu Y, Liu Z, Chen Q, Li L Expression of tumor-associated calcium signal transducer 2 in patients with salivary adenoid cystic carcinoma: Correlation with clinicopathological features and prognosis. Oncol Lett, 2014;8(4):1670-1674. 2014 [PMID: 25202389] (IHC-P, Human) IHC-P Human
Show All 16 Publications.

Review for TROP-2 Antibody (AF650) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF650:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Anonymous
ICC Human 01/09/2015
View

Summary

ApplicationImmunocytochemistry
Sample TestedSee PMID 23151048
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23151048

Details

Detection NotesSee PMID 23151048

Comments

CommentsPublished: See PMID 23151048Independent review from antibodyresource.com

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Secondary Antibodies

 

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Biotin BAF650

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Recent Reviews

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Anonymous
01/09/2015
Application: ICC
Species: Human

Bioinformatics

Gene Symbol TACSTD2
Uniprot