TROP-2 Antibody

Western blot shows lysates of NCI‑N87 human gastric carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TROP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF650) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TROP‑2 at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

PC‑3 human prostate cancer cell line was stained with Goat Anti-Human TROP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF650, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

TROP‑2 was detected in immersion fixed paraffin-embedded sections of human brain (frontal cortex) using Goat Anti-Human TROP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF650) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, Flow, IHC, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

TROP-2 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human TROP-2
  Thr88-Thr274
  Accession # P09758
• Specificity: Detects human TROP-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) MCAM, rhNCAM‑L1, and rhBCAM is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TACSTD2
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TROP-2 Antibody

• Cell surface glycoprotein Trop-2
• EGP1
• EGP-1
• epithelial glycoprotein-1
• GA733-1
• GA733-1GP50
• gastrointestinal tumor-associated antigen GA7331
• gp50
• M1S1cell surface glycoprotein TROP2
• Membrane component chromosome 1 surface marker 1
• membrane component, chromosome 1, surface marker 1
• Pancreatic carcinoma marker protein GA733-1
• pancreatic carcinoma marker protein GA7331,40kD glycoprotein, identified by monoclonal GA733
• T16
• TACSTD2
• TROP2
• TROP-2
• TROP2GA7331
• tumor-associated calcium signal transducer 2

Background

Human TROP-2, also called tumor associated calcium signal transducer 2 (TACSTD2), GA733-1, gp50 and T16, is a type I cell surface glycoprotein that is highly expressed on human carcinomas. It was originally identified as an antigen present on human gastrointestinal tumors and is the second of two members of this family. The other family member is GA733-2, also called EpCAM, TROP-1, 17-1A, gp40 and KSA. The TROP-2 gene is unique in that it contains no introns. A study of these two genes suggested that TROP-2 was the result of a retroposition of the EpCAM gene. TROP-2 and EpCAM share approximately 49% amino acid identity and approximately 67% similarity. Human and mouse TROP-2 share 87% similarity. The human TROP-2 protein consists of a putative 26 amino acid (aa) signal sequence, a 248 aa extracellular domain, a 23 aa transmembrane region and a 26 aa cytoplasmic domain. TROP-2 is capable of transducing an intracellular calcium signal and may play a role in tumor growth. It also has adhesive functions.

1. Linnenbach, A.J. et al. (1989) Proc. Natl. Acad. Sci. USA 86:27.
2. Linnenbach, A.J. et al. (1993) Mol. Cell. Biol. 13:1507.
3. Fornaro, M. et al. (1995) Int. J. Cancer 62:610.
4. Ripani, E. et al. (1998) Int. J. Cancer 76:671.
5. El Sewedy, T. et al. (1998) Int. J. Cancer 75:324.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TROP-2 Antibody (AF650)(18)

Publications using AF650

• JY Seok, X Fan TROP-2 and 5hmC expression in follicular-patterned thyroid neoplasm emphasizing tiny well-formed papillae Annals of diagnostic pathology, 2022;57(0):151903. 2022 [PMID: 35121239] (IHC, Human)
• PR Kamble, SR Patkar, AA Breed, BR Pathak N-glycosylation status of Trop2 impacts its surface density, interaction with claudin-7 and exosomal release Archives of biochemistry and biophysics, 2021;714(0):109084. 2021 [PMID: 34774484] (Western Blot, Human)
• Y Hao, D Zhang, Y Guo, Z Fu, D Yu, G Guan miR-488-3p sponged by circ-0000495 and mediated upregulation of TROP2 in head and neck squamous cell carcinoma development J Cancer, 2020;11(11):3375-3386. 2020 [PMID: 32231744] (IHC, Human)
• B Zeybek, A Manzano, A Bianchi, E Bonazzoli, S Bellone, N Buza, P Hui, S Lopez, E Perrone, P Manara, L Zammataro, G Altwerger, C Han, J Tymon-Rosa, G Menderes, E Ratner, DA Silasi, GS Huang, M Azodi, PE Schwartz, A Santin Cervical carcinomas that overexpress human trophoblast cell-surface marker (Trop-2) are highly sensitive to the antibody-drug conjugate sacituzumab govitecan Sci Rep, 2020;10(1):973. 2020 [PMID: 31969666] (IHC, Human)
• X Kuai, L Jia, T Yang, X Huang, W Zhao, M Zhang, Y Chen, J Zhu, Z Feng, Q Tang Trop2 Promotes Multidrug Resistance by Regulating Notch1 Signaling Pathway in Gastric Cancer Cells Med. Sci. Monit., 2020;26(0):e919566. 2020 [PMID: 31964857] (Western Blot, Human)
• C Han, E Perrone, B Zeybek, S Bellone, J Tymon-Rosa, G Altwerger, G Menderes, J Feinberg, K Haines, ME Muller Kar, A Bianchi, L Zammataro, A Manzano, E Bonazzoli, P Manara, N Buza, P Hui, E Ratner, DA Silasi, GS Huang, M Azodi, PE Schwartz, S Lopez, AD Santin In�vitro and in�vivo activity of sacituzumab govitecan, an antibody-drug conjugate targeting trophoblast cell-surface antigen 2 (Trop-2) in uterine serous carcinoma Gynecol. Oncol., 2019;0(0):. 2019 [PMID: 31839338] (IHC, Human)
• STK Sin, Y Li, M Liu, S Ma, XY Guan TROP-2 exhibits tumor suppressive functions in cervical cancer by dual inhibition of IGF-1R and ALK signaling Gynecol. Oncol., 2018;0(0):. 2018 [PMID: 30429055] (Western Blot, Human)
• S Vranic, J Palazzo, S Sanati, E Florento, E Contreras, J Xiu, J Swensen, Z Gatalica Potential Novel Therapy Targets in Neuroendocrine Carcinomas of the Breast Clin. Breast Cancer, 2018;0(0):. 2018 [PMID: 30268765] (IHC, Human)
• SM Zimmers, EP Browne, KE Williams, RM Jawale, CN Otis, SS Schneider, KF Arcaro TROP2 methylation and expression in tamoxifen-resistant breast cancer Cancer Cell Int., 2018;18(0):94. 2018 [PMID: 30002602] (IHC, Human)
• H Guan, Z Guo, W Liang, H Li, G Wei, L Xu, H Xiao, Y Li Trop2 enhances invasion of thyroid cancer by inducing MMP2 through ERK and JNK pathways BMC Cancer, 2017;17(1):486. 2017 [PMID: 28709407] (IHC, Human)
• Starodub A, Ocean A, Shah M, Guarino M, Picozzi V, Vahdat L, Thomas S, Govindan S, Maliakal P, Wegener W, Hamburger S, Sharkey R, Goldenberg D First-in-Human Trial of a Novel Anti-Trop-2 Antibody-SN-38 Conjugate, Sacituzumab Govitecan, for the Treatment of Diverse Metastatic Solid Tumors. Clin Cancer Res, 2015;21(17):3870-8. 2015 [PMID: 25944802] (IHC, Human)
• Xia Y, Li B, Gao N, Xia H, Men Y, Liu Y, Liu Z, Chen Q, Li L Expression of tumor-associated calcium signal transducer 2 in patients with salivary adenoid cystic carcinoma: Correlation with clinicopathological features and prognosis. Oncol Lett, 2014;8(4):1670-1674. 2014 [PMID: 25202389] (IHC-P, Human)
• Ambrogi , Federico, Fornili , Marco, Boracchi , Patrizia, Trerotola , Marco, Relli , Valeria, Simeone , Pasquale, La Sorda , Rossana, Lattanzio , Rossano, Querzoli , Patrizia, Pedriali , Massimo, Piantelli , Mauro, Biganzoli , Elia, Alberti , Saverio Trop-2 is a determinant of breast cancer survival. PLoS ONE, 2014;9(5):e96993. 2014 [PMID: 24824621] (Flow Cytometry, Human)
• Trerotola , M, Cantanelli , P, Guerra , E, Tripaldi , R, Aloisi , A L, Bonasera , V, Lattanzio , R, de Lange , R, Weidle , U H, Piantelli , M, Alberti , S Upregulation of Trop-2 quantitatively stimulates human cancer growth. Oncogene, 2013;32(2):222-33. 2013 [PMID: 22349828] (ICC, Human)
• Raji R, Guzzo F, Carrara L Uterine and ovarian carcinosarcomas overexpressing Trop-2 are sensitive to hRS7, a humanized anti-Trop-2 antibody. J. Exp. Clin. Cancer Res., 2011;30(0):106. 2011 [PMID: 22075385] (IHC-P, Human)
• Eisenwort G, Jurkin J, Yasmin N, Bauer T, Gesslbauer B, Strobl H Identification of TROP2 (TACSTD2), an EpCAM-like molecule, as a specific marker for TGF-beta1-dependent human epidermal Langerhans cells. J. Invest. Dermatol., 2011;131(10):2049-57. 2011 [PMID: 21677668] (Flow Cytometry, Human)
• Nakatsukasa M, Kawasaki S, Yamasaki K Tumor-associated calcium signal transducer 2 is required for the proper subcellular localization of claudin 1 and 7: implications in the pathogenesis of gelatinous drop-like corneal dystrophy. Am. J. Pathol., 2010;177(3):1344-55. 2010 [PMID: 20651236] (Immunoprecipitation, Human)
• Wang J, Day R, Dong Y, Weintraub SJ, Michel L Identification of Trop-2 as an oncogene and an attractive therapeutic target in colon cancers. Mol. Cancer Ther., 2008;7(2):280-5. 2008 [PMID: 18281513] (Neutralization, Mouse)

Review for TROP-2 Antibody (AF650) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Anonymous - ICC Human 01/09/2015

Summary

• Application: Immunocytochemistry
• Sample Tested: See PMID 23151048
• Species: Human

Bioinformatics

• Gene Symbol: TACSTD2
• Uniprot:
  • P09758()