ACD Antibody (1D8-1B6)

Images

 
Western Blot: ACD Antibody (1D8-1B6) [H00065057-M02] - Western Blot analysis of ACD expression in IMR-32 ( Cat # L008V1 ).
Immunocytochemistry/ Immunofluorescence: ACD Antibody (1D8-1B6) [H00065057-M02] - Analysis of monoclonal antibody to ACD on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: ACD Antibody (1D8-1B6) [H00065057-M02] - Analysis of monoclonal antibody to ACD on formalin-fixed paraffin-embedded human heart tissue. Antibody concentration 2 ug/ml.
Sandwich ELISA: ACD Antibody (1D8-1B6) [H00065057-M02] - Detection limit for recombinant GST tagged ACD is 0.3 ng/ml as a capture antibody.
Immunoprecipitation: ACD Antibody (1D8-1B6) [H00065057-M02] - Analysis of ACD transfected lysate using anti-ACD monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ACD MaxPab rabbit polyclonal ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ChIP, ELISA, ICC/IF, IHC, IHC-P, IP
Clone
1D8-1B6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

ACD Antibody (1D8-1B6) Summary

Immunogen
ACD (AAH16904, 1 a.a. ~ 545 a.a) full length recombinant protein with GST tag.MPGRCQSDAAMRVNGPASRAPAGWTSGSLHTGPRAGRPRAQARGVRGRGLLLRPRPAKELPLPRKGGAWAPAGNPGPLHPLGVAVGMAGSGRLVLRPWIRELILGSETPSSPRAGQLLEVLQDAEAAVAGPSHAPDTSDVGATLLVSDGTHSVRCLVTREALDTSDWEEKEFGFRGTEGRLLLLQDCGVHVQVAEGGAPAEFYLQVDRFSLLPTEQPRLRVPGCNQDLDVQKKLYDCLEEHLSESTSSNAGLSLS
Specificity
ACD - adrenocortical dysplasia homolog (mouse)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ACD
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Chromatin Immunoprecipitation 1:10-1:500
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA. Use in chromatin immunoprecipitation reported in scientific literature (PMID 25233904)
Reviewed Applications
Read 1 Review rated 3
using
H00065057-M02 in the following applications:

Publications
Read Publications using
H00065057-M02 in the following applications:

Reactivity Notes

Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ACD Antibody (1D8-1B6)

  • adrenocortical dysplasia homolog (mouse)
  • Pip1
  • PIP1Tint1
  • POT1 and TIN2 organizing protein
  • POT1 and TIN2-interacting protein
  • Ptop
  • PTOPTpp1
  • TIN2 interacting protein 1
  • TINT1adrenocortical dysplasia protein homolog
  • TPP1

Background

This gene encodes a protein that is involved in telomere function. This protein is one of six core proteins in the telosome/shelterin telomeric complex, which functions to maintain telomere length and to protect telomere ends. Through its interaction with other components, this protein plays a key role in the assembly and stabilization of this complex, and it mediates the access of telomerase to the telomere. Multiple transcript variants encoding different isoforms have been found for this gene. This gene, which is also referred to as TPP1, is distinct from the unrelated TPP1 gene on chromosome 11, which encodes tripeptidyl-peptidase I.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-56506
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, CyTOF-ready
NB500-176
Species: Hu
Applications: WB
NB600-1522
Species: Hu, Mu
Applications: WB, GS, ICC/IF
NBP1-85487
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-47914
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NB110-68281
Species: Hu, Mu, Rt, Mk
Applications: ICC/IF (-), WB, Simple Western, ELISA, Flow
NBP1-31133
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-292
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP1-89296
Species: Hu
Applications: WB, IHC, IHC-P
NB100-317
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NBP2-22527
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP1-76548
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC
NBP2-45388
Species: Hu
Applications: WB, IHC, IHC-P
H00065057-M02
Species: Hu
Applications: WB, ChIP, ELISA, ICC/IF, IHC, IHC-P, IP

Publications for ACD Antibody (H00065057-M02)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ChIP.


Filter By Application
ChIP
(1)
All Applications
Filter By Species
Human
(2)
All Species

Review for ACD Antibody (H00065057-M02) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using H00065057-M02:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot ACD H00065057-M02
Enlarge
reviewed by:
Anonymous
WB Human 09/01/2015
View

Summary

ApplicationWestern Blot
Sample Testedhuman lung homogenates
SpeciesHuman
LotD5201-S1

Blocking

Blocking Details5% BSA for 1 hr RT

Primary Anitbody

Dilution Ratio1:1000 in 5% BSA at 4 degree overnight

Secondary Antibody

Secondary DescriptionGoat anti-mouse IgG HRP secondary Ab
Secondary Manufacturer Cat#Bio-Rad Cat # 170-6516
Secondary Concentration1:10,000

Details

Detection NotesECL based detection using ChemiDoc MP for 1-2 min exposure

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for ACD Antibody (H00065057-M02) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional ACD Products

Bioinformatics Tool for ACD Antibody (H00065057-M02)

Discover related pathways, diseases and genes to ACD Antibody (H00065057-M02). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ACD Antibody (H00065057-M02)

Discover more about diseases related to ACD Antibody (H00065057-M02).
 

Pathways for ACD Antibody (H00065057-M02)

View related products by pathway.

PTMs for ACD Antibody (H00065057-M02)

Learn more about PTMs related to ACD Antibody (H00065057-M02).

Blogs on ACD.

Application Highlight: Recent uses of TERF2 in immunofluorescence (IF)
Telomeres are a region of repeat nucleotide sequences located at the end of chromosomes to protect our DNA from becoming damaged via end-to-end fusion.  TERF2, or telomeric-repeat binding factor 2, is important for telomere integrity and aids in th...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

3
5
0
4
0
3
1
2
0
1
0

Anonymous
09/01/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol ACD
Entrez
OMIM
Uniprot