BDNF Antibody

Images

 
Western Blot: BDNF Antibody [NBP1-46750] - Human platelet lysate. Blocking: 1% LFDM for 30 min at RT; primary antibody incubated at 4C overnight. Click on eLAB BOOK tab to see the details. Mature BDNF: (1:2000 dilution) ...read more
Immunohistochemistry-Paraffin: BDNF Antibody [NBP1-46750] - IHC-P on paraffin sections of rat hippocampus.The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for ...read more
Immunohistochemistry-Paraffin: BDNF Antibody [NBP1-46750] - Paraffin sections of rat spinal cord. Dilution 1: 1000.
Immunohistochemistry-Paraffin: BDNF Antibody [NBP1-46750] - Paraffin sections of rat hippocampus. Dilution 1: 1000.
Immunohistochemistry-Paraffin: BDNF Antibody [NBP1-46750] - Paraffin sections of mouse hippocampus. Dilution 1: 1000.
Immunohistochemistry-Paraffin: BDNF Antibody [NBP1-46750] - Paraffin sections of rat hippocampus. Dilution 1: 1000.
Immunohistochemistry-Paraffin: BDNF Antibody [NBP1-46750] - IHC-P on paraffin sections of rat hippocampus.The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

BDNF Antibody Summary

Immunogen
A synthetic peptide from internal region of human mature BDNF (BDNF, Brain-derived neurotrophic factor, Abrineurin) conjugated to an immunogenic carrier protein was used as the antigen.
Specificity
Specific for mature BDNF.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BDNF
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1000 - 1 : 2000
  • Immunohistochemistry 1000 - 1 : 2000
  • Immunohistochemistry-Paraffin 1000 - 1 : 2000
Reviewed Applications
Read 1 Review rated 3
using
NBP1-46750 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
No Preservative
Concentration
LYOPH
Purity
Unpurified
Reconstitution Instructions
Reconstitute in 100 ul of sterile water.

Alternate Names for BDNF Antibody

  • Abrineurin
  • ANON2
  • BDNF
  • brain-derived neurotrophic factor
  • BULN2
  • MGC34632
  • neurotrophin

Background

BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycaemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Subunit: Monomers and homodimers. Binds to NTRK2/TRKB. Subcellular Location: Secreted protein. Post translation modification: Converted into mature BDNF by plasmin (PLG). Similarity: Belongs to the NGF-beta family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF175
Species: Hu
Applications: WB, Flow, IHC, CyTOF-ready, Neut
NBP1-47897
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
MAB367
Species: Hu, Ca
Applications: WB, IHC, ICC
NBP1-87382
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
AF1404
Species: Mu, Rt
Applications: WB, Simple Western, IHC, Block
557-NT/CF
Species: Rt
NB110-40561
Species: Hu
Applications: WB, IP
NBP2-13883
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
AF5789
Species: Hu, Mu, Rt
Applications: WB
MAB224
Species: Hu
Applications: Flow, IHC, CyTOF-ready, Neut
NBP1-18910
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, IP
AF1138
Species: Hu
Applications: WB, Flow, IHC, CyTOF-ready, Neut
NBP1-46750
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P

Publications for BDNF Antibody (NBP1-46750) (0)

There are no publications for BDNF Antibody (NBP1-46750).
By submitting your publication information earn gift cards and discounts for future purchases.

Review for BDNF Antibody (NBP1-46750) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Rat.
We have 1 review tested in 1 application: IHC-Fr.

Reviews using NBP1-46750:
Filter by Applications
IHC-Fr
(1)
All Applications
Filter by Species
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen BDNF NBP1-46750
Enlarge
reviewed by:
Ra Gyung Kim
IHC-Fr Rat 10/16/2017
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Testedrat brain sections
SpeciesRat
LotRb.2728-080516-WS

Comments

CommentsThis figure represents immunofluoro (Alexa 555)-labeled BDNF signals using antibody NBP1-46750 (1:200). When we overlayed images with BDNF and NeuN signals, they showed colocalization, and we confirmed those signals were positive. However, the patterns are ring-shaped, different from what we expected. This was immunohistochemistry with frozen rat brain section.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for BDNF Antibody (NBP1-46750) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional BDNF Products

Bioinformatics Tool for BDNF Antibody (NBP1-46750)

Discover related pathways, diseases and genes to BDNF Antibody (NBP1-46750). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for BDNF Antibody (NBP1-46750)

Discover more about diseases related to BDNF Antibody (NBP1-46750).
 

Pathways for BDNF Antibody (NBP1-46750)

View related products by pathway.

PTMs for BDNF Antibody (NBP1-46750)

Learn more about PTMs related to BDNF Antibody (NBP1-46750).
 

Research Areas for BDNF Antibody (NBP1-46750)

Find related products by research area.

Blogs on BDNF.

The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

The identification of dopaminergic neurons using Tyrosine Hydroxylase in Parkinson's research and LRRK2
Tyrosine hydroxylase (TH) is a crucial enzyme involved in the biosynthesis of dopamine, norepinephrine and epinephrine in the brain.  Specifically, TH catalyzes the conversion of l-tyrosine to l-dihydroxyphenylalanine (l-dopa).  The importance of t...  Read full blog post.

Niemann Pick-C1 and cholesterol dynamics
Niemann-Pick type C1 (NPC1) mediates low-density cholesterol transport from late endosomes and lysosomes to other areas of the cell via receptor mediation endocytosis.  Although cholesterol moves freely inside the cell, it cannot independently expo...  Read full blog post.

Synapsin I: Implicated in synaptic activity across a diverse range of studies
Synapsins are a family of neuronal proteins that are most renowned for their activity in modulating the pre-synaptic terminal.  Synapsin’s behavior is regulated by protein kinases and phosphatases, which alter the way that synapsin’s i...  Read full blog post.

TrkB: Bridging Ontogenesis and Oncogenesis
Tropomyosin receptor kinase B (TrkB) is a member of the Trk receptor tyrosine kinases family consisting of TrkA, TrkB and TrkC. The sequence of these family members is highly conserved. Interaction of brain-derived neurotrophic factor (BDNF) with its...  Read full blog post.

TrkB and Nervous System Function
Neutrophins and their receptors play an important role in regulating the development of both the central and peripheral nervous systems. Neurotrophin ligand binding to each of their respective Trk cellular receptors is essential for the growth and sur...  Read full blog post.

TrkB: Docking for Neurotrophins and Beyond.
Tropomyosin receptor kinase B (TrkB) is a member of the Trk receptor tyrosine kinases family consisting of TrkA, TrkB and TrkC. The sequence of these family members is highly conserved. TrK's are activated by several neurotrophins, which are small pro...  Read full blog post.

BDNF Antibodies Aid Research on Alzheimer's Therapies
Brain-derived neurotrophic factor (BDNF) is known to be important for neuronal differentiation, survival, migration and plasticity in both the developing embryo and adult synapses. The BDNF antibody is also proving to be an important tool in Alzheimer...  Read full blog post.

BDNF Antibodies and Synaptic Research
Brain-derived neurotrophic factor (BDNF) is a member of the NGF family of neurotrophins. During development it regulates the survival and differentiation of neuronal cell populations in the central and peripheral nervous system, while in adult synapse...  Read full blog post.

Save Time & Money with High Quality Sandwich ELISA Kits
Enzyme-linked immunosorbent assay (ELISA) is a widely used technique for detecting concentration of proteins, using enzyme tagged antibodies with which react with dyes to produce a colorimetric or fluorescent signal. Sandwich ELISA takes this one step...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

3
5
0
4
0
3
1
2
0
1
0

Ra Gyung Kim
10/16/2017
Application: IHC-Fr
Species: Rat

Bioinformatics

Gene Symbol BDNF
Entrez