BDNF Antibody

Western Blot: BDNF Antibody [NB100-98682] - BDNF lowered the mechanical withdrawal threshold further and promoted activation of astrocytes and microglia, and enhanced the p38/JNK pathway to aggravate the release of IL-1beta and TNF-alpha in the SDH of CYP-induced cystitis. Western blots showing the expression of BDNF was further upregulated when compared with the CYP + PBS group. Data of mechanical withdrawal threshold were analyzed using a two-way analysis of variance (ANOVA) followed by the Sidak's multiple comparisons test. All data were calculated as mean +/- SEM (n = 10 per group). **p < 0.01, ***p < 0.001 vs. the control group. #p < 0.05, ##p < 0.01, ###p < 0.001 vs. the CYP + rBDNF group. $p < 0.05, $$p < 0.01, $$$p < 0.001 vs. the CYP + PBS group.

Immunohistochemistry: BDNF Antibody [NB100-98682] - Effects of mBMSC and EA treatment on the expression of mBDNF and NT4 in neurons. Photomicrographs for mBDNF/NeuN or NT4/NeuN double-positive cells in the striatum and hippocampus (n = 5). The number of mBDNF/NeuN double-positive cells in the SVZ + striatum and hippocampus was significantly higher in the EA-treated MCAO group and the combined mBMSC + EA-treated MCAO group, compared to the number in the other groups. The number of NT4/NeuN double-positive cells in the hippocampus was significantly higher in the EA-treated MCAO group than in the vehicle-treated MCAO group. #p < 0.05 and ##p < 0.01 versus control group; *p < 0.05, **p < 0.01, and ***p < 0.001 versus vehicle-treated MCAO group; &&p < 0.01 versus mBMSC-treated MCAO group. Scale bar = 100 um. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41598-018-20481-3) licensed under a CC-BY license.

Western Blot: BDNF Antibody [NB100-98682] - Human platelet lysate and recombinant mature BDNF. Blocking: 1% LFDM for 30 min at RT; primary antibody (1:1000) incubated at 4C overnight.

Immunohistochemistry-Paraffin: BDNF Antibody [NB100-98682] - Mouse hippocampus. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 1000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.

Immunohistochemistry-Paraffin: BDNF Antibody [NB100-98682] - Mouse hippocampus. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 1000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.

Immunohistochemistry-Paraffin: BDNF Antibody [NB100-98682] - Mouse hippocampus. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 1000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.

Western Blot: BDNF Antibody [NB100-98682] - Effect of treatment with FX extract on CREB/BDNF signaling. (a-c) Differences in the phosphorylation of CREB & expression of BDNF between groups in the PFC & hippocampus (n = 3-4). (d, e) pCREB & BDNF immunofluorescence was assessed in the PFC & hippocampus (n = 3-4). Mean ± SD. #P < 0.05 versus normal group; ∗P < 0.05 & ∗∗P < 0.01 versus control group. Scale bar = 200 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30065945), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: BDNF Antibody [NB100-98682] - Effect of treatment with FX extract on CREB/BDNF signaling. (a-c) Differences in the phosphorylation of CREB & expression of BDNF between groups in the PFC & hippocampus (n = 3-4). (d, e) pCREB & BDNF immunofluorescence was assessed in the PFC & hippocampus (n = 3-4). Mean ± SD. #P < 0.05 versus normal group; ∗P < 0.05 & ∗∗P < 0.01 versus control group. Scale bar = 200 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30065945), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: BDNF Antibody [NB100-98682] - Antagonized TrkB could restrain the activation of astrocytes & microglia & supress the p38/JNK pathway to alleviate the release of IL-1 beta & TNF-alpha in the SDH of CYP-induced cystitis. Western blots showed that the overexpression of a BDNF, b TrkB, cp-TrkB, d Iba1, e GFAP, fp-p38, gp-JNK, h TNF-alpha , & i IL-1 beta were downregulated in comparison to the CYP + DMSO group after ANA-12 treatment. All data were calculated as mean ± SEM (n = 5 per group). **p < 0.01, ***p < 0.001 vs. control group. ##p < 0.01, ###p < 0.001 vs. CYP + ANA-12 group Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31931832), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: BDNF Antibody [NB100-98682] - BDNF lowered the mechanical withdrawal threshold further & promoted activation of astrocytes & microglia, & enhanced the p38/JNK pathway to aggravate the release of IL-1 beta & TNF-alpha in the SDH of CYP-induced cystitis. a BDNF treated every other day after CYP injection could further lower the mechanical withdrawal threshold & suppress the retrieval of mechanical threshold when compared with the CYP + PBS group. After the exogenous BDNF injection, Western blots showing the expression of b BDNF, c TrkB, dp-TrkB, e Iba1, f GFAP, gp-p38, hp-JNK, i TNF-alpha , & j IL-1 beta were all further upregulated when compared with the CYP + PBS group. Data of mechanical withdrawal threshold were analyzed using a two-way analysis of variance (ANOVA) followed by the Sidak's multiple comparisons test. All data were calculated as mean ± SEM (n = 10 per group). **p < 0.01, ***p < 0.001 vs. the control group. #p < 0.05, ##p < 0.01, ###p < 0.001 vs. the CYP + rBDNF group. $p < 0.05, $$p < 0.01, $$$p < 0.001 vs. the CYP + PBS group Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31931832), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: BDNF Antibody [NB100-98682] - Effects of mBMSC & EA treatment on the expression of trophic factors. (A) Photomicrographs & (B‒D) histograms for mBDNF, NT4, & VEGF expression (n = 4–5). mBDNF expression was markedly increased by treatment with mBMSC and/or EA compared to vehicle treatment. mBDNF expression levels were higher in the EA-treated MCAO group than in the mBMSC-treated MCAO group. NT4 expression in the SVZ & hippocampus was significantly higher in the combined mBMSC & EA treatment group than in the other groups. VEGF expression in the hippocampus was markedly higher in the mBMSC treatment group than in the vehicle-treated MCAO group. #p < 0.05, ##p < 0.01, & ###p < 0.001 versus control group; *p < 0.05, **p < 0.01, & ***p < 0.001 versus vehicle-treated MCAO group; &p < 0.05, &&p < 0.01, & &&&p < 0.001 versus mBMSC-treated MCAO group; Scale bar = 1 mm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29391466), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: BDNF Antibody [NB100-98682] - The expression of BDNF-TrkB signaling in the SDH of CYP-induced cystitis. a Changes of the mechanical threshold in CYP-induced cystitis model. Compared to that in the control group, the mechanical threshold of the cystitis group decreased significantly after the CYP injection & remained low until day 17, & the minimum threshold value was reached on day 12. The expression of b BDNF, c TrkB, & dp-TrkB were evaluated by western blots. Compared to the control group, they were upregulated on days 8, 12, & 17. e Immunofluorescence double staining assay of BDNF & p-TrkB in the SDH. BDNF & p-TrkB (red), NeuN, GFAP, & OX-42( green), co-localization (yellow). BDNF was mainly colocalized in neurons which mainly located in Laminate II to IV. & TrkB receptors expressed in neurons, microglia, & astrocytes. The white dotted lines in picture “BDNF/NeuN” showed the laminate of the SDH according to Rexed & Steiner. Scale bar = 100 μm. All data were calculated as mean ± SEM (n = 5 per group). *p < 0.05, ** p < 0.01, *** p < 0.001 vs. the control group Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31931832), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Ze
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

BDNF Antibody Summary

• Immunogen: A synthetic peptide from n-terminal region of human mature BDNF conjugated to blue carrier protein was used as the antigen. The peptide is homologous in many other species including rat, mouse, zebra fish and xenopus.
• Specificity: Specific for mature BDNF.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BDNF
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Paraffin 1:1000
  • Western Blot 1:1000
• Application Notes: Use in IHC-P reported in scientific literature (PMID: 30222997). ICC/IF reported in scientific literature (PMID: 27914953).
• Theoretical MW: 28 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  BDNF Overexpression Lysate

Reactivity Notes

Zebrafish reactivity reported in scientific literature (PMID: 30222997).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from whole antisera
• Preservative: No Preservative
• Purity: Unpurified
• Reconstitution Instructions: Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Alternate Names for BDNF Antibody

• Abrineurin
• ANON2
• BDNF
• brain-derived neurotrophic factor
• BULN2
• MGC34632
• Neurotrophin

Background

FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BDNF Antibody (NB100-98682)(37)

Publications using NB100-98682

• Gadsielinski M, Gladyszz D, Gralewski M Relationship between BDNF-positive number of nerve fibers and pain in intervertebral disc degeneration Sciforum 2023-01-01 (ICC/IF)
• Poon CH, Liu Y, Pak S et al. Prelimbic Cortical Stimulation with L-methionine Enhances Cognition through Hippocampal DNA Methylation and Neuroplasticity Mechanisms Aging and disease 2023-02-01 [PMID: 36818556] (WB, Rat)
• Doboszewska U, Soca?a K, Pier�g M et al. Dietary Zinc Differentially Regulates the Effects of the GPR39 Receptor Agonist, TC-G 1008, in the Maximal Electroshock Seizure Test and Pentylenetetrazole-Kindling Model of Epilepsy Cells 2023-01-09 [PMID: 36672199] (Western Blot, Mouse)
• Wu CY, Zhang Y, Howard P et Al. ACSL3 is a promising therapeutic target for alleviating anxiety and depression in Alzheimer's disease Geroscience 2024-11-13 [PMID: 39532829]
• Tikhonova, MA;Shvaikovskaya, AA;Zhanaeva, SY;Moysak, GI;Akopyan, AA;Rzaev, JA;Danilenko, KV;Aftanas, LI; Concordance between the In Vivo Content of Neurospecific Proteins (BDNF, NSE, VILIP-1, S100B) in the Hippocampus and Blood in Patients with Epilepsy International journal of molecular sciences 2023-12-29 [PMID: 38203674]
• Gao Q, Gao Z, Su M et al. Umbilical Cord Mesenchymal Stem Cells Overexpressing Heme Oxygenase-1 Promotes Symptoms Recovery in Cystitis Rats by Alleviating Neuroinflammation Stem cells international 2023-11-14 [PMID: 38020203]
• Filimonova EA, Pashkov AA, Moysak GI et al. Brain but not serum BDNF levels are associated with structural alterations in the hippocampal regions in patients with drug-resistant mesial temporal lobe epilepsy Frontiers in Neuroscience 2023-07-19 [PMID: 37539386] (Western Blot, Mouse)
• Doboszewska U, Soca?a K, Pieróg M et al. TC-G 1008 facilitates epileptogenesis by acting selectively at the GPR39 receptor but non-selectively activates CREB in the hippocampus of pentylenetetrazole-kindled mice Cellular and Molecular Life Sciences 2023-05-01 [PMID: 37185787] (Western Blot, Mouse)
• Staszkiewicz R, G?adysz D, Gralewski M et al. Usefulness of Detecting Brain-Derived Neurotrophic Factor in Intervertebral Disc Degeneration of the Lumbosacral Spine Medical science monitor : international medical journal of experimental and clinical research 2023-01-16 [PMID: 36642939] (IHC-Fr, Human)
  
  Details:
  1:200 dilution
• Wang M, Wang T, Ji H et al. Modulation effect of non-invasive transcranial ultrasound stimulation in an ADHD rat model Journal of neural engineering 2023-01-18 [PMID: 36599159]
• Gao F, Wang J, Yang S et al. Fear extinction induced by activation of PKA ameliorates anxiety-like behavior in PTSD mice Neuropharmacology 2023-01-01 [PMID: 36341808] (WB, Mouse)
  
  Details:
  Dilution used in WB 1:1000
• Ma W, Wei X, Gu H et al. Intra-amniotic transplantation of brain-derived neurotrophic factor-modified mesenchymal stem cells treatment for rat fetuses with spina bifida aperta Stem cell research & therapy 2022-08-13 [PMID: 35964077]
  
  Details:
  Dilution used 1:25, Outbred Wistar rats
• Doboszewska U, Socala K, PierOg M et al. The Effects and Mechanisms of Action of TC-G 1008, GPR39 Agonist, in Animal Models of Seizures and Epilepsy Cells 2022-07-09 [PMID: 35805072]
• Saleem S, Banerjee R, Rajesh Kannan R Chrysin-Loaded Chitosan Nanoparticle-Mediated Neuroprotection in A beta 1-42-Induced Neurodegenerative Conditions in Zebrafish ACS chemical neuroscience 2022-06-13 [PMID: 35696319]
• Fanarioti E, Tsarouchi M, Vasilakopoulou PB et al. Brain polar phenol content, behavioural and neurochemical effects of Corinthian currant in a rotenone rat model of Parkinson's disease Nutritional neuroscience 2022-06-03 [PMID: 35656969]
• Pak M, Park y, yang H et al. Samhwangsasim-tang attenuates neuronal apoptosis and cognitive decline through BDNF-mediated activation of tyrosin kinase B and p75-neurotrophin receptors Phytomedicine 2022-02-01 [PMID: 35279612] (IF/IHC, Mouse)
• Gentry N, McMahon T, Yamazaki M et al. Microglia are involved in the protection of memories formed during sleep deprivation Neurobiology of Sleep and Circadian Rhythms 2022-05-01 [PMID: 35028489] (WB, Mouse)
• Wang J, Gao F, Cui S et al. Utility of 7,8-dihydroxyflavone in preventing astrocytic and synaptic deficits in the hippocampus elicited by PTSD Pharmacological research 2022-01-10 [PMID: 35026406] (WB, Mouse)
• Ferguson L, Giza CC, Serpa RO et al. Sex Differences in Neurophysiological Changes Following Voluntary Exercise in Adolescent Rats Frontiers in Neurology 2021-07-22 [PMID: 34367052] (Western Blot, Mouse)
• Topal A, Ozdemir S, Arslan H, Comakli S How does elevated water temperature affect fish brain? (A neurophysiological and experimental study: Assessment of brain derived neurotrophic factor, cFOS, apoptotic genes, heat shock genes, ER-stress genes and oxidative stress genes) Fish & shellfish immunology 2021-05-06 [PMID: 33965523]
• Jung, D H, Ahn, S M Et al. Therapeutic effects of anodal transcranial direct current stimulation in a rat model of ADHD. Elife 2020-09-21 [PMID: 32955434] (ICC/IF, Mouse)
• Wang X, Xu W, Chen H et al. Astragaloside IV prevents A beta 1-42 oligomers-induced memory impairment and hippocampal cell apoptosis by promoting PPAR gamma/BDNF signaling pathway Brain Res. 2020-07-30 [PMID: 32739157] (WB, Mouse)
• Celik H, Kucukler S, Ozdemir S et al. Lycopene protects against central and peripheral neuropathy by inhibiting oxaliplatin-induced ATF-6 pathway, apoptosis, inflammation and oxidative stress in brains and sciatic tissues of rats Neurotoxicology 2020-06-13 [PMID: 32544411] (ICC/IF, WB, Rat)
• Song Z, Shen F, Zhang Z et al. Calpain inhibition ameliorates depression-like behaviors by reducing inflammation and promoting synaptic protein expression in the hippocampus Neuropharmacology 2020-05-31 [PMID: 32492450] (WB, Mouse)
• Ding H, Chen J, Su M et al. BDNF promotes activation of astrocytes and microglia contributing to neuroinflammation and mechanical allodynia in cyclophosphamide-induced cystitis J Neuroinflammation 2020-01-13 [PMID: 31931832] (WB, Rat)
• Comakli S, Kokturk M, Topal A et al. Immunofluorescence/fluorescence assessment of brain-derived neurotrophic factor, c-Fos activation, and apoptosis in the brain of zebrafish (Danio rerio) larvae exposed to glufosinate. Neurotoxicology 2018-09-14 [PMID: 30222997] (IHC-P, Zebrafish)
• Kim Y R, Park B K et al. Antidepressant Effect of Fraxinus rhynchophylla Hance Extract in a Mouse Model of Chronic Stress-Induced Depression. Biomed Res Int 2018-02-08 [PMID: 30065945] (WB, IF/IHC, Mouse)
• Kim Y, Park B, Kim Y et al. Antidepressant Effect of Fraxinus rhynchophylla Hance Extract in a Mouse Model of Chronic Stress-Induced Depression BioMed Research International 2018-07-03 [PMID: 30046601] (IF/IHC, Mouse)
• Kim YR, Ahn SM, Pak ME, Lee HJ. Potential benefits of mesenchymal stem cells and electroacupuncture on the trophic factors associated with neurogenesis in mice with ischemic stroke. Sci Rep. 2018-02-01 [PMID: 29391466] (ICC/IF, Mouse)
• Pak ME, Jung DH, Lee HJ et al. Combined therapy involving electroacupuncture and treadmill exercise attenuates demyelination in the corpus callosum by stimulating oligodendrogenesis in a rat model of neonatal hypoxia-ischemia. Exp. Neurol. 2018-02-01 [PMID: 29199131] (Rat)
• Tang Y, Liu L, Xu D et al. Interaction between astrocytic colony stimulating factor and its receptor on microglia mediates central sensitization and behavioral hypersensitivity in chronic post ischemic pain model. Brain Behav Immun 2018-02-01 [PMID: 29080683]
• Nam SM, Kim JW, Kwon HJ et al. Differential Effects of Low- and High-dose Zinc Supplementation on Synaptic Plasticity and Neurogenesis in the Hippocampus of Control and High-fat Diet-fed Mice Neurochem. Res. 2017-08-02 [PMID: 28770438] (WB, Mouse)
• Lee HI, Lee SW, Kim NG et al. Low-level light emitting diode therapy promotes long-term functional recovery after experimental stroke in mice. J Biophotonics 2017-05-02 [PMID: 28464523] (Mouse)
  
  Details:
  The BDNF antibody was used to investigate the efficacy of using low level LED therapy in encouraging brain function recover of mice post stroke.
• Atasoy IL, Dursun E, Gezen-Ak D et al. Both secreted and the cellular levels of BDNF attenuated due to tau hyperphosphorylation in primary cultures of cortical neurons. J. Chem. Neuroanat. 2016-11-30 [PMID: 27914953] (ICC/IF, Rat)
• Efstathopoulos P, Kourgiantaki A, Karali K et al. Fingolimod induces neurogenesis in adult mouse hippocampus and improves contextual fear memory. Translational Psychiatry 2015-01-01 [PMID: 26795749] (WB, Mouse)

Review for BDNF Antibody (NB100-98682) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Verified Customer WB Rat 04/23/2025

Summary

• Application: Western Blot
• Sample Tested: Rat hippocampus
• Species: Rat
• Lot: Rb2851-090916-WS

Comments

• Comments: There are three aging groups on this blot: rats fed a Mediterranean diet, rats fed a western diet, and rats fed a chow diet. Animals were perfused with PBS and brains were micro dissected and frozen at -80C until needed. Dilution was 1:1,000. Two bands are clearly noticeable at ~32 and 30 kDa, while one band at ~13 kDa was less noticeable.

Bioinformatics

• Gene Symbol: BDNF
• Entrez:
  • 627(Human)
  • 12064(Mouse)
  • 24225(Rat)
• Uniprot:
  • P23560()