BDNF Antibody

Western Blot: BDNF Antibody [NB100-98682] - Human platelet lysate and recombinant mature BDNF. Blocking: 1% LFDM for 30 min at RT; primary antibody (1:1000) incubated at 4C overnight.

Immunohistochemistry-Paraffin: BDNF Antibody [NB100-98682] - Mouse hippocampus. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 1000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.

Immunohistochemistry-Paraffin: BDNF Antibody [NB100-98682] - Mouse hippocampus. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 1000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.

Immunohistochemistry-Paraffin: BDNF Antibody [NB100-98682] - Mouse hippocampus. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 1000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.

• Reactivity: Hu, Mu, Rt, Ze
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

BDNF Antibody Summary

• Immunogen: A synthetic peptide from human mature BDNF conjugated to an immunogenic carrier protein was used as the immunogen. The peptide is homologous in many other species including rat, mouse, zebrafish and xenopus.
• Specificity: Specific for mature BDNF.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BDNF
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:300-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin
• Application Notes: Use in IHC-P reported in scientific literature (PMID: 30222997). ICC/IF reported in scientific literature (PMID: 27914953).
• Theoretical MW: 28 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  BDNF Overexpression Lysate

Reactivity Notes

Zebrafish reactivity reported in scientific literature (PMID: 30222997).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: No Preservative
• Purity: Unpurified
• Reconstitution Instructions: Please note the larger size(s) come in lyophilized form and the sample size will be in liquid formate. Reconstitute with 0.1 ml sterilized water to desired concentration.

Alternate Names for BDNF Antibody

• Abrineurin
• ANON2
• BDNF
• brain-derived neurotrophic factor
• BULN2
• MGC34632
• Neurotrophin

Background

FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BDNF Antibody (NB100-98682)(12)

Publications using NB100-98682

• Kim YR, Ahn SM, Pak ME, Lee HJ. Potential benefits of mesenchymal stem cells and electroacupuncture on the trophic factors associated with neurogenesis in mice with ischemic stroke. Sci Rep. 2018 Feb 01 [PMID: 29391466] (ICC/IF, Mouse)
• Pak ME, Jung DH, Lee HJ et al. Combined therapy involving electroacupuncture and treadmill exercise attenuates demyelination in the corpus callosum by stimulating oligodendrogenesis in a rat model of neonatal hypoxia-ischemia. Exp. Neurol. 2018 Feb 01 [PMID: 29199131] (Rat)
• Lee HI, Lee SW, Kim NG et al. Low-level light emitting diode therapy promotes long-term functional recovery after experimental stroke in mice. J Biophotonics May 2 2017 [PMID: 28464523] (Mouse)
  
  Details:
  The BDNF antibody was used to investigate the efficacy of using low level LED therapy in encouraging brain function recover of mice post stroke.
• Atasoy IL, Dursun E, Gezen-Ak D et al. Both secreted and the cellular levels of BDNF attenuated due to tau hyperphosphorylation in primary cultures of cortical neurons. J. Chem. Neuroanat. Nov 30 2016 [PMID: 27914953] (ICC/IF, Rat)
• Ding H, Chen J, Su M et al. BDNF promotes activation of astrocytes and microglia contributing to neuroinflammation and mechanical allodynia in cyclophosphamide-induced cystitis J Neuroinflammation Jan 13 2020 [PMID: 31931832] (WB, Rat)
• Comakli S, Kokturk M, Topal A et al. Immunofluorescence/fluorescence assessment of brain-derived neurotrophic factor, c-Fos activation, and apoptosis in the brain of zebrafish (Danio rerio) larvae exposed to glufosinate. Neurotoxicology Sep 14 2018 [PMID: 30222997] (IHC-P, Zebrafish)
• Kim Y, Park B, Kim Y et al. Antidepressant Effect of Fraxinus rhynchophylla Hance Extract in a Mouse Model of Chronic Stress-Induced Depression BioMed Research International Jul 3 2018 [PMID: 30046601] (IHC, Mouse)
• Tang Y, Liu L, Xu D et al. Interaction between astrocytic colony stimulating factor and its receptor on microglia mediates central sensitization and behavioral hypersensitivity in chronic post ischemic pain model. Brain Behav Immun 2018 Feb [PMID: 29080683]
• Nam SM, Kim JW, Kwon HJ et al. Differential Effects of Low- and High-dose Zinc Supplementation on Synaptic Plasticity and Neurogenesis in the Hippocampus of Control and High-fat Diet-fed Mice Neurochem. Res. 2017 Aug 02 [PMID: 28770438] (WB, Mouse)
• Efstathopoulos P, Kourgiantaki A, Karali K et al. Fingolimod induces neurogenesis in adult mouse hippocampus and improves contextual fear memory. Translational Psychiatry 2015 [PMID: 26795749] (WB, Mouse)

Bioinformatics

• Gene Symbol: BDNF
• Entrez:
  • 627(Human)
  • 12064(Mouse)
  • 24225(Rat)
• Uniprot:
  • P23560()