BDNF Antibody

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Biological Strategies: Western Blot: BDNF Antibody [NB100-98682] - BDNF lowered the mechanical withdrawal threshold further and promoted activation of astrocytes and microglia, and enhanced the p38/JNK pathway to ...read more
Immunohistochemistry: BDNF Antibody [NB100-98682] - Effects of mBMSC and EA treatment on the expression of mBDNF and NT4 in neurons. Photomicrographs for mBDNF/NeuN or NT4/NeuN double-positive cells in the striatum and ...read more
Western Blot: BDNF Antibody [NB100-98682] - Human platelet lysate and recombinant mature BDNF. Blocking: 1% LFDM for 30 min at RT; primary antibody (1:1000) incubated at 4C overnight.
Immunohistochemistry-Paraffin: BDNF Antibody [NB100-98682] - Mouse hippocampus. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 ...read more
Immunohistochemistry-Paraffin: BDNF Antibody [NB100-98682] - Mouse hippocampus. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 ...read more
Immunohistochemistry-Paraffin: BDNF Antibody [NB100-98682] - Mouse hippocampus. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, ZeSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
   

Biological Strategies

     

Order Details

BDNF Antibody Summary

Immunogen
A synthetic peptide from human mature BDNF conjugated to an immunogenic carrier protein was used as the immunogen. The peptide is homologous in many other species including rat, mouse, zebrafish and xenopus.
Specificity
Specific for mature BDNF.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BDNF
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:300-1:2000
  • Western Blot 1:300-1:2000
Application Notes
Use in IHC-P reported in scientific literature (PMID: 30222997). ICC/IF reported in scientific literature (PMID: 27914953).
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
BDNF Overexpression Lysate
Publications
Read Publications using
NB100-98682 in the following applications:

  • 4 publications
  • IHC
    3 publications
  • 1 publication
  • WB
    11 publications

Reactivity Notes

Zebrafish reactivity reported in scientific literature (PMID: 30222997).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
No Preservative
Purity
Unpurified
Reconstitution Instructions
Please note the larger size(s) come in lyophilized form and the sample size will be in liquid formate. Reconstitute with 0.1 ml sterilized water to desired concentration.

Alternate Names for BDNF Antibody

  • Abrineurin
  • ANON2
  • BDNF
  • brain-derived neurotrophic factor
  • BULN2
  • MGC34632
  • Neurotrophin

Background

FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BDNF Antibody (NB100-98682)(23)

We have publications tested in 3 confirmed species: Mouse, Rat, Zebrafish.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-P, WB.


Filter By Application
ICC/IF
(4)
IHC
(3)
IHC-P
(1)
WB
(11)
All Applications
Filter By Species
Mouse
(13)
Rat
(4)
Zebrafish
(1)
All Species
Showing Publications 1 - 10 of 23. Show All 23 Publications.
Publications using NB100-98682 Applications Species
Ma W, Wei X, Gu H et al. Intra-Amniotic Transplantation of Brain-Derived Neurotrophic Factor - Modified Mesenchymal Stem Cells Treatment for Rat Fetuses with Spina Bifida Aperta Research Square Mar 8 2022 (WB) WB
Pak M, Park y, yang H et al. Samhwangsasim-tang attenuates neuronal apoptosis and cognitive decline through BDNF-mediated activation of tyrosin kinase B and p75-neurotrophin receptors Phytomedicine Feb 1 2022 [PMID: 35279612] (IHC, Mouse) IHC Mouse
Wang J, Gao F, Cui S et al. Utility of 7,8-dihydroxyflavone in preventing astrocytic and synaptic deficits in the hippocampus elicited by PTSD Pharmacological research Jan 10 2022 [PMID: 35026406] (WB, Mouse) WB Mouse
Song Z, Shen F, Zhang Z et al. Calpain inhibition ameliorates depression-like behaviors by reducing inflammation and promoting synaptic protein expression in the hippocampus Neuropharmacology May 31 2020 [PMID: 32492450] (WB, Mouse) WB Mouse
Doboszewska U, Socala K, PierOg M et al. The Effects and Mechanisms of Action of TC-G 1008, GPR39 Agonist, in Animal Models of Seizures and Epilepsy Research Square Jan 7 2022 (WB, Mouse) WB Mouse
Gentry N, McMahon T, Yamazaki M et al. Microglia are involved in the protection of memories formed during sleep deprivation Neurobiology of Sleep and Circadian Rhythms May 1 2022 [PMID: 35028489] (WB, Mouse) WB Mouse
Topal A, Ozdemir S, Arslan H, Comakli S How does elevated water temperature affect fish brain? (A neurophysiological and experimental study: Assessment of brain derived neurotrophic factor, cFOS, apoptotic genes, heat shock genes, ER-stress genes and oxidative stress genes) Fish & shellfish immunology May 6 2021 [PMID: 33965523]
Jung, D H, Ahn, S M Et al. Therapeutic effects of anodal transcranial direct current stimulation in a rat model of ADHD. Elife Sep 21 2020 [PMID: 32955434] (ICC/IF, Mouse) ICC/IF Mouse
Wang X, Xu W, Chen H et al. Astragaloside IV prevents A beta 1-42 oligomers-induced memory impairment and hippocampal cell apoptosis by promoting PPAR gamma/BDNF signaling pathway Brain Res. Jul 30 2020 [PMID: 32739157] (WB, Mouse) WB Mouse
Celik H, Kucukler S, Ozdemir S et al. Lycopene protects against central and peripheral neuropathy by inhibiting oxaliplatin-induced ATF-6 pathway, apoptosis, inflammation and oxidative stress in brains and sciatic tissues of rats Neurotoxicology Jun 13 2020 [PMID: 32544411] (WB, ICC/IF, Rat) WB, ICC/IF Rat
Show All 23 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for BDNF Antibody (NB100-98682) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional BDNF Products

Bioinformatics Tool for BDNF Antibody (NB100-98682)

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Blogs on BDNF. Showing 1-10 of 11 blog posts - Show all blog posts.


  Read full blog post.

The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

The identification of dopaminergic neurons using Tyrosine Hydroxylase in Parkinson's research and LRRK2
Tyrosine hydroxylase (TH) is a crucial enzyme involved in the biosynthesis of dopamine, norepinephrine and epinephrine in the brain.  Specifically, TH catalyzes the conversion of l-tyrosine to l-dihydroxyphenylalanine (l-dopa).  The importance of t...  Read full blog post.

Niemann Pick-C1 and cholesterol dynamics
Niemann-Pick type C1 (NPC1) mediates low-density cholesterol transport from late endosomes and lysosomes to other areas of the cell via receptor mediation endocytosis.  Although cholesterol moves freely inside the cell, it cannot independently expo...  Read full blog post.

Synapsin I: Implicated in synaptic activity across a diverse range of studies
Synapsins are a family of neuronal proteins that are most renowned for their activity in modulating the pre-synaptic terminal.  Synapsin’s behavior is regulated by protein kinases and phosphatases, which alter the way that synapsin’s i...  Read full blog post.

TrkB: Bridging Ontogenesis and Oncogenesis
Tropomyosin receptor kinase B (TrkB) is a member of the Trk receptor tyrosine kinases family consisting of TrkA, TrkB and TrkC. The sequence of these family members is highly conserved. Interaction of brain-derived neurotrophic factor (BDNF) with its ...  Read full blog post.

TrkB and Nervous System Function
Neutrophins and their receptors play an important role in regulating the development of both the central and peripheral nervous systems. Neurotrophin ligand binding to each of their respective Trk cellular receptors is essential for the growth and sur...  Read full blog post.

TrkB: Docking for Neurotrophins and Beyond.
Tropomyosin receptor kinase B (TrkB) is a member of the Trk receptor tyrosine kinases family consisting of TrkA, TrkB and TrkC. The sequence of these family members is highly conserved. TrK's are activated by several neurotrophins, which are small pro...  Read full blog post.

BDNF Antibodies Aid Research on Alzheimer's Therapies
Brain-derived neurotrophic factor (BDNF) is known to be important for neuronal differentiation, survival, migration and plasticity in both the developing embryo and adult synapses. The BDNF antibody is also proving to be an important tool in Alzheimer...  Read full blog post.

BDNF Antibodies and Synaptic Research
Brain-derived neurotrophic factor (BDNF) is a member of the NGF family of neurotrophins. During development it regulates the survival and differentiation of neuronal cell populations in the central and peripheral nervous system, while in adult synapse...  Read full blog post.

Showing 1-10 of 11 blog posts - Show all blog posts.
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Bioinformatics

Gene Symbol BDNF
Entrez
Uniprot