![Western Blot: BDNF Antibody (3D9C5) [NBP2-37276] - Analysis using BDNF mouse mAb against SK-N-SH (1) cell lysate.](http://images.novusbio.com/fullsize2/BDNF-Antibody-(3D9C5)-Western-Blot-NBP2-37276-img0004.jpg "Western Blot: BDNF Antibody (3D9C5) [NBP2-37276] - Analysis using BDNF mouse mAb against SK-N-SH (1) cell lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF |
| Clone | 3D9C5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | Purified recombinant fragment of human BDNF (AA: 19-248) expressed in E. Coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | BDNF |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 27.8 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Diseases for BDNF Antibody (NBP2-37276)Discover more about diseases related to BDNF Antibody (NBP2-37276).
| Pathways for BDNF Antibody (NBP2-37276)View related products by pathway.
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PTMs for BDNF Antibody (NBP2-37276)Learn more about PTMs related to BDNF Antibody (NBP2-37276).
| Research Areas for BDNF Antibody (NBP2-37276)Find related products by research area.
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The identification of dopaminergic neurons using Tyrosine Hydroxylase in Parkinson's research and LRRK2 Tyrosine hydroxylase (TH) is a crucial enzyme involved in the biosynthesis of dopamine, norepinephrine and epinephrine in the brain. Specifically, TH catalyzes the conversion of l-tyrosine to l-dihydroxyphenylalanine (l-dopa). The importance of t... Read full blog post. |
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Niemann Pick-C1 and cholesterol dynamics Niemann-Pick type C1 (NPC1) mediates low-density cholesterol transport from late endosomes and lysosomes to other areas of the cell via receptor mediation endocytosis. Although cholesterol moves freely inside the cell, it cannot independently expo... Read full blog post. |
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Synapsin I: Implicated in synaptic activity across a diverse range of studies Synapsins are a family of neuronal proteins that are most renowned for their activity in modulating the pre-synaptic terminal. Synapsin’s behavior is regulated by protein kinases and phosphatases, which alter the way that synapsin’s i... Read full blog post. |
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TrkB: Bridging Ontogenesis and Oncogenesis Tropomyosin receptor kinase B (TrkB) is a member of the Trk receptor tyrosine kinases family consisting of TrkA, TrkB and TrkC. The sequence of these family members is highly conserved. Interaction of brain-derived neurotrophic factor (BDNF) with its... Read full blog post. |
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TrkB and Nervous System Function Neutrophins and their receptors play an important role in regulating the development of both the central and peripheral nervous systems. Neurotrophin ligand binding to each of their respective Trk cellular receptors is essential for the growth and sur... Read full blog post. |
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TrkB: Docking for Neurotrophins and Beyond. Tropomyosin receptor kinase B (TrkB) is a member of the Trk receptor tyrosine kinases family consisting of TrkA, TrkB and TrkC. The sequence of these family members is highly conserved. TrK's are activated by several neurotrophins, which are small pro... Read full blog post. |
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BDNF Antibodies Aid Research on Alzheimer's Therapies Brain-derived neurotrophic factor (BDNF) is known to be important for neuronal differentiation, survival, migration and plasticity in both the developing embryo and adult synapses. The BDNF antibody is also proving to be an important tool in Alzheimer... Read full blog post. |
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BDNF Antibodies and Synaptic Research Brain-derived neurotrophic factor (BDNF) is a member of the NGF family of neurotrophins. During development it regulates the survival and differentiation of neuronal cell populations in the central and peripheral nervous system, while in adult synapse... Read full blog post. |
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| Gene Symbol | BDNF |
![Immunocytochemistry/Immunofluorescence: BDNF Antibody (3D9C5) [NBP2-37276] - Analysis of Hela cells using BDNF mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize2/BDNF-Antibody-(3D9C5)-Immunocytochemistry-Immunofluorescence-NBP2-37276-img0005.jpg "Immunocytochemistry/Immunofluorescence: BDNF Antibody (3D9C5) [NBP2-37276] - Analysis of Hela cells using BDNF mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.")
![Western Blot: BDNF Antibody (3D9C5) [NBP2-37276] - Analysis using BDNF mAb against human BDNF (AA: 19-248) recombinant protein. (Expected MW is 51.7 kDa)](http://images.novusbio.com/fullsize2/BDNF-Antibody-(3D9C5)-Western-Blot-NBP2-37276-img0002.jpg "Western Blot: BDNF Antibody (3D9C5) [NBP2-37276] - Analysis using BDNF mAb against human BDNF (AA: 19-248) recombinant protein. (Expected MW is 51.7 kDa)")
![Western Blot: BDNF Antibody (3D9C5) [NBP2-37276] - Analysis using BDNF mAb against HEK293 (1) and BDNF (AA: 19-248)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize2/BDNF-Antibody-(3D9C5)-Western-Blot-NBP2-37276-img0003.jpg "Western Blot: BDNF Antibody (3D9C5) [NBP2-37276] - Analysis using BDNF mAb against HEK293 (1) and BDNF (AA: 19-248)-hIgGFc transfected HEK293 (2) cell lysate.")
![ELISA: BDNF Antibody (3D9C5) [NBP2-37276] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize2/BDNF-Antibody-(3D9C5)-ELISA-NBP2-37276-img0001.jpg "ELISA: BDNF Antibody (3D9C5) [NBP2-37276] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);")
![Western Blot: tropomyosin-3 Antibody [NBP1-87382] - Analysis in human cell line THP-1.](http://images.novusbio.com/fullsize2/tropomyosin-3-Antibody-Western-Blot-NBP1-87382-img0006.jpg)
![Immunohistochemistry-Paraffin: tropomyosin-3 Antibody [NBP1-87382] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize2/tropomyosin-3-Antibody-Immunohistochemistry-Paraffin-NBP1-87382-img0008.jpg)
![Western Blot: tropomyosin-3 Antibody [NBP1-87382] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize2/tropomyosin-3 Antibody-Western Blot-NBP1-87382-img0007.jpg)
![Western Blot: CDP/CUTL1 Antibody [NBP2-13883] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted)](http://images.novusbio.com/fullsize2/CDP-CUTL1-Antibody-Western-Blot-NBP2-13883-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: CDP/CUTL1 Antibody [NBP2-13883] - Staining of human cell line MCF7 shows localization to nucleoplasm & the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/CDP-CUTL1 Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13883-img0006.jpg)
![Immunohistochemistry-Paraffin: CDP/CUTL1 Antibody [NBP2-13883] - Staining of human stomach, upper shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize2/CDP-CUTL1-Antibody-Immunohistochemistry-Paraffin-NBP2-13883-img0001.jpg)
![Western Blot: HCLS1 Antibody (1A8) [H00003059-M02] - HCLS1 monoclonal antibody (M02), clone 1A8 Analysis of HCLS1 expression in K-562.](http://images.novusbio.com/fullsize2/HCLS1-Antibody-(1A8)-Western-Blot-H00003059-M02-img0013.jpg)
![Immunohistochemistry-Paraffin: HCLS1 Antibody (1A8) [H00003059-M02] - Analysis of monoclonal antibody to HCLS1 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize2/HCLS1-Antibody-(1A8)-Immunohistochemistry-Paraffin-H00003059-M02-img0010.jpg)
![Western Blot: HCLS1 Antibody (1A8) [H00003059-M02] - Analysis of HCLS1 over-expressed 293 cell line, cotransfected with HCLS1 Validated Chimera RNAi ( Cat # H00003059-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with HCLS1 monoclonal antibody (M02), clone 1A8 (Cat # H00003059-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize2/HCLS1-Antibody-(1A8)-Western-Blot-H00003059-M02-img0011.jpg)
![Simple Western: KHSRP Antibody [NBP1-18910] - Simple Western lane view shows a specific band for KHSRP in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize2/KHSRP-Antibody-Simple-Western-NBP1-18910-img0005.jpg)
![Immunohistochemistry: KHSRP Antibody [NBP1-18910] - Immunofluorescence of ILF3 and KHSRP in the retina. Retinal sections were prepared from control mice and from mice at one day after light exposure and stained for GS (green) and KHSRP (red). Image collected and cropped by CiteAb from the following publication (http://bmcbiol.biomedcentral.com/articles/10.1186/s12915-015-0137-1), licensed under a CC-BY licence.](http://images.novusbio.com/fullsize2/KHSRP Antibody-Immunocytochemistry-Immunofluorescence-NBP1-18910-img0009.jpg)
![Western Blot: KHSRP Antibody [NBP1-18910] - Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. KSRP was also immunoprecipitated by rabbit anti-KSRP antibody NBP1-18911.](http://images.novusbio.com/fullsize2/KHSRP-Antibody-Western-Blot-NBP1-18910-img0001.jpg)
![SDS-Page: Mouse IgG1 Isotype Control [NBP1-97005] - Lane 1:, Non-reduced. M: Opal Pre-stained Ladder. Lane 2:, Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](http://images.novusbio.com/images2/Mouse-IgG1-Isotype-Control-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control [NBP1-97005] - Lane 1:, Non-reduced. M: Opal Pre-stained Ladder. Lane 2:, Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")