Recombinant Human Siglec-7/CD328 Fc Chimera Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human Siglec-7/CD328 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of human red blood cells. Kelm, S. et al. (1994) Current Biology 4:965. The ED50 for this effect is 0.5‑2 µg/mL.
Source
Mouse myeloma cell line, NS0-derived human Siglec-7/CD328 protein
Human Siglec-7
(Gln19-Gly357)
Accession # Q9Y286
DIEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
No results obtained: Gln19 predicted
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
SIGLEC7
Purity
>85%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
64 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
90-100 kDa, reducing conditions
Publications
Read Publications using
1138-SL in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Purity
>85%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Siglec-7/CD328 Fc Chimera Protein, CF

  • Adhesion inhibitory receptor molecule 1
  • adhesion inhibitory receptor molecule 1, siglec-7
  • AIRM1
  • AIRM-1
  • AIRM1QA79 membrane protein
  • CD328 antigen
  • CD328
  • CDw328
  • D-siglec
  • p75
  • p75/AIRM1
  • QA79
  • sialic acid binding Ig-like lectin 7
  • sialic acid binding immunoglobulin-like lectin 7
  • sialic acid-binding Ig-like lectin 7
  • Siglec7
  • Siglec-7

Background

Siglecs (1) (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains (1, 2). Eleven human Siglecs have been cloned and characterized. They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglecs 5 to 11 (1 - 4). To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. Siglecs 5 to 11 share a high degree of sequence similarity with CD33/Siglec-3 both in their extracellular and intracellular regions. They are collectively referred to as CD33-related Siglecs. One remarkable feature of the CD33-related Siglecs is their differential expression pattern within the hematopoietic system (2, 3). This fact, together with the presence of two conserved immunoreceptor tyrosine-based inhibition motifs (ITIMs) in their cytoplasmic tails, suggests that CD33-related Siglecs are involved in the regulation of cellular activation within the immune system.

Human Siglec-7 encodes a 467 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-like V-type domain, two Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail (5). Siglec-7 exists as a monomer on the cell surface and is expressed on natural killer cells, CD8+ T cells and monocytes (3, 5). It binds equally well to both alpha 2,3- and alpha 2,6-linked sialic acid (5). The gene encoding Siglec-7 was mapped to chromosome 19q13.3.

  1. Crocker, P.R. et al. (1998) Glycobiology 8:v.
  2. Crocker, P.R. and A. Varki (2001) Trends Immunol. 22:337.
  3. Crocker, P.R. and A. Varki (2001) Immunology 103:137.
  4. Angata, T. et al. (2002) J. Biol. Chem. 277:24466.
  5. Nicoll, G. et al. (1999) J. Biol. Chem. 274:34089.

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Publications for Siglec-7/CD328 (1138-SL)(4)

We have publications tested in 3 confirmed species: Human, Bacteria - E. Coli, Virus.

We have publications tested in 3 applications: Binding Assay, Bioassay, Surface Plasmon Resonance.


Filter By Application
Binding Assay
(1)
Bioassay
(1)
Surface Plasmon Resonance
(1)
All Applications
Filter By Species
Human
(1)
Bacteria - E. Coli
(1)
Virus
(1)
All Species

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Bioinformatics

Gene Symbol SIGLEC7
Uniprot