Autophagy Antibody Pack

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Genetic Strategies: Knockout Validated: Autophagy Antibody Pack [NB910-94877] - Lysates of HeLa parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 ...read more
Genetic Strategies: Knockout Validated: Autophagy Antibody Pack [NB910-94877] - LC3B was detected in immersion fixed Cloroquine treated HeLa cells (left) but was not detected in LC3B knockout HeLa cells (right) ...read more
Genetic Strategies: Knockout Validated: Autophagy Antibody Pack [NB910-94877] - ERK phosphorylation in Atg5-/-cells depends on nutrient availability. Serum-deprived Atg5-/- MEFs display decreased ERK and p90RSK ...read more
Immunohistochemistry: Autophagy Antibody Pack [NB910-94877] - Staining of ATG5 using NB110-53818 in human hepatocytes (liver) at 2.5ug/ml. 40X magnification.
Simple Western: Autophagy Antibody Pack [NB910-94877] - Lane view shows a specific band for Beclin using NB110-87318 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 ...read more
Flow Cytometry: Autophagy Antibody Pack [NB910-94877] - An intracellular stain was performed on Hela Cells with NB110-56893 to detect ATG9 and a matched isotype control. Cells were fixed with 4% PFA and then ...read more
Immunohistochemistry: Autophagy Antibody Pack [NB910-94877] - Analysis of a FFPE tissue section of human breast cancer using Beclin 1 antibody (NB110-87318) at 1:300 dilution, followed by a HRP labeled secondary ...read more
Biological Strategies: Immunohistochemistry: Autophagy Antibody Pack [NB910-94877] - SCI leads to lysosomal dysfunction. Representative high magnification images ( x 60) of LC3 (NB100-2220, green) and CTSD (red) ...read more
Western Blot: Autophagy Antibody Pack [NB910-94877] - Total protein from human HeLa and mouse Neuro2A cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. ...read more
Western Blot: Autophagy Antibody Pack [NB910-94877] - Detection of ATG16L1 using NB110-60928 in HeLa whole cell extracts.
Immunohistochemistry: Autophagy Antibody Pack [NB910-94877] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human colon cancer using ATG16L1 antibody (NB110-60928) at 1:500 on a Bond Rx autostainer (Leica ...read more
Genetic Strategies: Western Blot: Autophagy Antibody Pack [NB910-94877] - Detection of ATG9A protein using NB110-56893 in HEK293 lysates. Lane 1: siRNA ATG9A knockdown Lane 2: wildtype ATG9A.
Western Blot: Autophagy Antibody Pack [NB910-94877] - CD133 promoted glucose uptake. E. Detection of autophagic genes in Huh-7con and Huh-7sh133 cells by Western blotting. Image collected and cropped by CiteAb from the ...read more
Immunohistochemistry: Autophagy Antibody Pack [NB910-94877] - Detection of ATG9A (NB110-56893) in mouse intestine.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Al, Bv, PmSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IP, KD

Order Details

Autophagy Antibody Pack Summary

Description
This pack contains 1 vial each of: NB100-2220 (25 uL), NB110-87318 (25 uL), NB110-53818 (25 uL), NB110-56893 (25 uL) and NB110-82384 (25 uL).
Immunogen
See individual datasheets
Specificity
Autophagy Antibodies (Beclin, LC3, ATG5, ATG9, ATG16L1)
Gene
BECN1

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Knockdown Validated
  • Simple Western
  • Western Blot
Application Notes
See individual datasheets of components for their validated applications
Publications
Read Publication using
NB910-94877 in the following applications:

  • WB
    1 publication

Reactivity Notes

See individual datasheets of components for their validated species

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
See individual datasheets

Kit Components

Alternate Names for Autophagy Antibody Pack

  • ATG6 autophagy related 6 homolog
  • ATG6
  • beclin 1 (coiled-coil, moesin-like BCL2 interacting protein)
  • beclin 1 (coiled-coil, moesin-like BCL2-interacting protein)
  • beclin 1, autophagy related
  • beclin1
  • beclin-1
  • Coiled-coil myosin-like BCL2-interacting protein
  • GT197
  • Protein GT197
  • VPS30

Background

This Autophagy Antibodies Pack includes sample aliquots to many different autophagy related proteins.
Autophagy can be understood as a cell recycling system on its most basic level. Components of the cytoplasm are sequestered and moved in the lysosome where they are broken down into their basic components and returned to the cytosol for reuse.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.

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Species: Am, Ca, Fi, Hu, Mu, Pl, Rt, Ze
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NB100-56098
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
NB110-53818
Species: Al, Bv, Dr, Fi, Gp, Hu, Mu, Po, Pm, Rt, Xp, Ze
Applications: EM, ELISA, Flow, IB, ICC/IF, IHC, IHC-P, IP, KD, KO, PLA, RIA, Simple Western, WB
NB600-607
Species: Hu, Rt
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NB100-56104
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-Fr, IHC-P, IP, WB
H00008878-M01
Species: Bv, Ha, Hu, Mu, Rb, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
MAB6608
Species: Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow, Simple Western, WB
AF887
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
NB110-87320
Species: Hu, Mu, Po, Rt
Applications: ICC/IF, KD, Simple Western, WB
H00002965-M01
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, KD, S-ELISA, WB
NBP1-31381
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, WB
NBP2-37399
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NBP2-02627
Species: Ca, Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP1-19151
Species: Bv, Hu, Ma
Applications: ICC/IF, IHC, IHC-P, IP, WB
NBP1-28566
Species: Bv, Hu, Pm, Mu, Po, Rt
Applications: Func, IHC, IHC-Fr, IHC-P, WB
NBP2-19804
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-88937
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-15071
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KO, WB
NB910-94877
Species: Hu, Mu, Rt, Po, Al, Bv, Pm
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IP, KD

Publications for Autophagy Antibody Pack (NB910-94877)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Blogs on Autophagy. Showing 1-10 of 63 blog posts - Show all blog posts.

Staufen1 Overabundance and the Consequent mTOR Hyperactivity in Amyotrophic Lateral Sclerosis, Spinocerebellar Ataxia Type 2, Alzheimer’s, Parkinson’s, and Huntington’s Diseases
Jamshed Arslan, Pharm D, PhD Neurodegenerative disorders involve loss of function and, ultimately, death of neurons. Selective neuronal vulnerability has been observed in a variety of neurodegenerative diseases. Fo...  Read full blog post.

Autophagy and RAS signaling: Clinical implications
By Christina Towers, PhD The cellular recycling process known as autophagy is currently being targeted in over 60 clinical trials focused on treating different types of cancer1. To date, the only autophagy-targeted ...  Read full blog post.

Methamphetamine with HIV induces mitochondrial dysfunction and neuronal injury through oxidative stress
By Jamshed Arslan, Pharm. D., PhD. December 1 is the World AIDS Day. Despite the combination antiretroviral therapy, 10-25% of Human Immunodeficiency Virus (HIV)-positive individuals report neurocognitive impairm...  Read full blog post.

Understanding Mitophagy Mechanisms: Canonical PINK1/Parkin, LC3-Dependent Piecemeal, and LC3-Independent Mitochondrial Derived Vesicles
By Christina Towers, PhD What is Mitophagy?The selective degradation of mitochondria via double membrane autophagosome vesicles is called mitophagy. Damaged mitochondria can generate harmful amounts of reactive ox...  Read full blog post.

Liver ASK1 activates autophagy to protect against hepatic fat accumulation, non-alcoholic steatohepatitis and fibrosis
By Jamshed Arslan, Pharm. D., PhD. The most common chronic liver disorder worldwide is non-alcoholic fatty liver disease (NAFLD). This obesity-linked disorder can manifest as hepatic fat accumulation (steatosis) wit...  Read full blog post.

New Players in the Mitophagy Game
By Christina Towers, PhD Mitochondrial turn over via the lysosome, otherwise known as mitophagy, involves engulfment of mitochondria into double membrane autophagosomes and subsequent fusion with lysosomes. Much is al...  Read full blog post.

Autophagy Research Update: What a difference a year makes!
By Christina Towers, PhD Over the last two decades the field of autophagy has exploded! Innovative techniques, comprehensive analysis and disease-relevant models have yielded basic and clinical discoveries of conseque...  Read full blog post.

Autophagy and Metastasis
  By Christina Towers, PhD The majority of cancer patients die from metastatic disease at secondary sites. The threshold to undergo metastasis is high. Only a minority of cancer cells acquire invasive phenotypes...  Read full blog post.

Optogenetic Control of Mitophagy: AMBRA1 based mitophagy switch
By Christina Towers, PhD Mitophagy in the BrainSelective autophagic degradation of damaged mitochondria, known as mitophagy, has been described as a cyto-protective process. Accordingly, defects in mitophagy h...  Read full blog post.

Autophagic flux: Is p62 a good indicator?
By Christina Towers, PhD Is p62 a good indicator of autophagic flux? The short answer: Yes … but … SQSTM1 encodes the cargo adaptor protein, p62, which interacts with autophagic substrates and delivers them to aut...  Read full blog post.

Showing 1-10 of 63 blog posts - Show all blog posts.

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Bioinformatics

Gene Symbol BECN1