Autophagy Antibody Pack

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Genetic Strategies: Knockout Validated: Autophagy Antibody Pack [NB910-94877] - Lysates of HeLa parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 ...read more
Genetic Strategies: Knockout Validated: Autophagy Antibody Pack [NB910-94877] - LC3B was detected in immersion fixed Cloroquine treated HeLa cells (left) but was not detected in LC3B knockout HeLa cells (right) ...read more
Genetic Strategies: Knockout Validated: Autophagy Antibody Pack [NB910-94877] - ERK phosphorylation in Atg5-/-cells depends on nutrient availability. Serum-deprived Atg5-/- MEFs display decreased ERK and p90RSK ...read more
Immunohistochemistry: Autophagy Antibody Pack [NB910-94877] - Staining of ATG5 using NB110-53818 in human hepatocytes (liver) at 2.5ug/ml. 40X magnification.
Simple Western: Autophagy Antibody Pack [NB910-94877] - Lane view shows a specific band for Beclin using NB110-87318 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 ...read more
Flow Cytometry: Autophagy Antibody Pack [NB910-94877] - An intracellular stain was performed on Hela Cells with NB110-56893 to detect ATG9 and a matched isotype control. Cells were fixed with 4% PFA and then ...read more
Immunohistochemistry: Autophagy Antibody Pack [NB910-94877] - Analysis of a FFPE tissue section of human breast cancer using Beclin 1 antibody (NB110-87318) at 1:300 dilution, followed by a HRP labeled secondary ...read more
Biological Strategies: Immunohistochemistry: Autophagy Antibody Pack [NB910-94877] - SCI leads to lysosomal dysfunction. Representative high magnification images ( x 60) of LC3 (NB100-2220, green) and CTSD (red) ...read more
Western Blot: Autophagy Antibody Pack [NB910-94877] - Total protein from human HeLa and mouse Neuro2A cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. ...read more
Western Blot: Autophagy Antibody Pack [NB910-94877] - Detection of ATG16L1 using NB110-60928 in HeLa whole cell extracts.
Immunohistochemistry: Autophagy Antibody Pack [NB910-94877] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human colon cancer using ATG16L1 antibody (NB110-60928) at 1:500 on a Bond Rx autostainer (Leica ...read more
Western Blot: Autophagy Antibody Pack [NB910-94877] - Detection of ATG9A protein using NB110-56893 in HEK293 lysates. Lane 1: siRNA ATG9A knockdown Lane 2: wildtype ATG9A.
Western Blot: Autophagy Antibody Pack [NB910-94877] - CD133 promoted glucose uptake. E. Detection of autophagic genes in Huh-7con and Huh-7sh133 cells by Western blotting. Image collected and cropped by CiteAb from the ...read more
Immunohistochemistry: Autophagy Antibody Pack [NB910-94877] - Detection of ATG9A (NB110-56893) in mouse intestine.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, KO

Order Details

Autophagy Antibody Pack Summary

Immunogen
See individual datasheets
Specificity
Autophagy Antibodies (Beclin, LC3, ATG5, ATG9, ATG16L1)
Gene
BECN1

Applications/Dilutions

Application Notes
Mainly western blot and immunohistochemistry on paraffin sections. However, additional applications may be possible for individual products.
See individual datasheets for validated applications.
Publications
Read Publication using
NB910-94877 in the following applications:

  • WB
    1 publication

Reactivity Notes

All vials are validated for mouse and human but individual vials may have additional reactivity, please see each vials data sheet for reactivity information specific to each product.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
See individual datasheets

Kit Components

Notes

This pack contains 1 vial each of: NB100-2220 (25 uL), NB110-87318 (25 uL), NB110-53818 (25 uL), NB110-56893 (25 uL) and NB110-82384 (25 uL).

Alternate Names for Autophagy Antibody Pack

  • ATG6 autophagy related 6 homolog
  • ATG6
  • beclin 1 (coiled-coil, moesin-like BCL2 interacting protein)
  • beclin 1 (coiled-coil, moesin-like BCL2-interacting protein)
  • beclin 1, autophagy related
  • beclin1
  • beclin-1
  • Coiled-coil myosin-like BCL2-interacting protein
  • GT197
  • Protein GT197
  • VPS30

Background

This Autophagy Antibodies Pack includes sample aliquots to many different autophagy related proteins.
Autophagy can be understood as a cell recycling system on its most basic level. Components of the cytoplasm are sequestered and moved in the lysosome where they are broken down into their basic components and returned to the cytosol for reuse.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.

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NBP1-28566
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NBP2-46403
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NBP2-88937
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NBP2-15071
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NB910-94877
Species: Hu, Mu
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, KO

Publications for Autophagy Antibody Pack (NB910-94877)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Autophagy Antibody Pack (NB910-94877) (0)

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Blogs on Autophagy. Showing 1-10 of 60 blog posts - Show all blog posts.

Understanding Mitophagy Mechanisms: Canonical PINK1/Parkin, LC3-Dependent Piecemeal, and LC3-Independent Mitochondrial Derived Vesicles
By Christina Towers, PhD What is Mitophagy?The selective degradation of mitochondria via double membrane autophagosome vesicles is called mitophagy. Damaged mitochondria can generate harmful amounts of reactive ox...  Read full blog post.

Liver ASK1 activates autophagy to protect against hepatic fat accumulation, non-alcoholic steatohepatitis and fibrosis
By Jamshed Arslan, Pharm. D., PhD. The most common chronic liver disorder worldwide is non-alcoholic fatty liver disease (NAFLD). This obesity-linked disorder can manifest as hepatic fat accumulation (steatosis) wit...  Read full blog post.

New Players in the Mitophagy Game
By Christina Towers, PhD Mitochondrial turn over via the lysosome, otherwise known as mitophagy, involves engulfment of mitochondria into double membrane autophagosomes and subsequent fusion with lysosomes. Much is al...  Read full blog post.

Autophagy Research Update: What a difference a year makes!
By Christina Towers, PhD Over the last two decades the field of autophagy has exploded! Innovative techniques, comprehensive analysis and disease-relevant models have yielded basic and clinical discoveries of conseque...  Read full blog post.

Autophagy and Metastasis
  By Christina Towers, PhD The majority of cancer patients die from metastatic disease at secondary sites. The threshold to undergo metastasis is high. Only a minority of cancer cells acquire invasive phenotypes...  Read full blog post.


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Mechanisms of neurodegeneration: Mitochondrial dysfunction and oxidative stress
By Michalina Hanzel, PhDIn this second installment of our three blog-posts series on major cellular mechanisms responsible for neurodegenerative disorders, we will explore the processes of mitochondrial dysfunction an...  Read full blog post.


  Read full blog post.

Showing 1-10 of 60 blog posts - Show all blog posts.

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Bioinformatics

Gene Symbol BECN1