Autophagy Antibody Pack

Images

 
Western Blot: Autophagy Antibody Pack [NB910-94877] - Detection of ATG16L1 in NIH/3T3 whole cell lysates.
Immunocytochemistry/ Immunofluorescence: Autophagy Antibody Pack [NB910-94877] - Staining of SY5Y cells at 1:250. Incubated overnight at 4 degrees. Photo courtesy of an anonymous collaborator.
Immunohistochemistry: Autophagy Antibody Pack [NB910-94877] - Staining of hepatocytes at 2.5ug/ml. Human liver hepatocytes 40X magnification.
Western Blot: Autophagy Antibody Pack [NB910-94877] - Detection of ATG5 in mouse wildtype ES cell lysate (Lane 1). Lane 2 is a mouse ATG5 KO ES cell lysate (negative control). Atg5-/- ES cells from Dr. Noboru Mizushima ...read more
Western Blot: Autophagy Antibody Pack [NB910-94877] - Detection of ATG9A protein in HEK293 lysates. Lane 1: siRNA ATG9A knockdown Lane 2: wildtype ATG9A.
Western Blot: Autophagy Antibody Pack [NB910-94877] - Detection of LC3 in human brain lysate. Atg5-/- ES cells from Dr. Noboru Mizushima [Mizushima, N. et al. J. Cell Biol. 152 (2001)] Photo courtesy of Dr. Beth Levine, ...read more
Western Blot: Autophagy Antibody Pack [NB910-94877] - Detection of ATG16L1 in NIH/3T3 whole cell lysates.
Western Blot: Autophagy Antibody Pack [NB910-94877] - Detection of Beclin 1 in HeLa whole cell lysate at 1:10,000.
Immunohistochemistry: Autophagy Antibody Pack [NB910-94877] - Staining of normal breast tissue.
Immunohistochemistry: Autophagy Antibody Pack [NB910-94877] - Staining of breast carcinoma.
Immunohistochemistry: Autophagy Antibody Pack [NB910-94877] - Staining of ATG9A in mouse intestine.
Immunohistochemistry: Autophagy Antibody Pack [NB910-94877] - Staining of brain, cerebral cortex, neurons with cell processes.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P

Order Details

Autophagy Antibody Pack Summary

Immunogen
See individual datasheets
Specificity
Autophagy Antibodies (Beclin, LC3, ATG5, ATG9, ATG16L1)
Gene
BECN1

Applications/Dilutions

Application Notes
Mainly western blot and immunohistochemistry on paraffin sections. However, additional applications may be possible for individual products.
Publications
Read Publication using
NB910-94877 in the following applications:

  • WB
    1 publication

Reactivity Notes

All vials are validated for mouse and human but individual vials may have additional reactivity, please see each vials data sheet for reactivity information specific to each product.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
See individual datasheets

Kit Components

Notes

25ul vials of each of following: NB100-2220, NB110-87318, NB110-53818, NB110-56893 and NB110-82384.

Alternate Names for Autophagy Antibody Pack

  • ATG6 autophagy related 6 homolog
  • ATG6
  • beclin 1 (coiled-coil, moesin-like BCL2 interacting protein)
  • beclin 1 (coiled-coil, moesin-like BCL2-interacting protein)
  • beclin 1, autophagy related
  • beclin1
  • beclin-1
  • Coiled-coil myosin-like BCL2-interacting protein
  • GT197
  • Protein GT197
  • VPS30

Background

This Autophagy Antibodies Pack includes sample aliquots to many different autophagy related proteins.
Autophagy can be understood as a cell recycling system on its most basic level. Components of the cytoplasm are sequestered and moved in the lysosome where they are broken down into their basic components and returned to the cytosol for reuse.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.

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NB910-94877
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P

Publications for Autophagy Antibody Pack (NB910-94877)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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WB
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Blogs on Autophagy. Showing 1-10 of 52 blog posts - Show all blog posts.

Autophagic flux: Is p62 a good indicator?
By Christina Towers, PhD Is p62 a good indicator of autophagic flux? The short answer: Yes … but … SQSTM1 encodes the cargo adaptor protein, p62, which interacts with autophagic substrates and delivers them to autopha...  Read full blog post.

Best genes to knockout to prevent autophagic flux
By Christina Towers, PhD Autophagy flux: Basic principlesAutophagic flux is defined as the amount of cellular material degraded and recycled through the process of autophagy. Briefly, the autophagy process involves...  Read full blog post.

Mechanisms of neurodegeneration: Mitochondrial dysfunction and oxidative stress
By Michalina Hanzel, PhDIn this second installment of our three blog-posts series on major cellular mechanisms responsible for neurodegenerative disorders, we will explore the processes of mitochondrial dysfunction an...  Read full blog post.

Make each cell count: Advanced techniques for assessing autophagy using flow cytometry
Kristy R. Howell, PhDThe cellular recycling process known as autophagy may be induced by a variety of conditions including reduced nutrient availability, serum starvation and pharmacological agents (e.g., Rapamycin)....  Read full blog post.

Mechanisms of Neurodegeneration: Protein aggregation and failure of autophagy
By Michalina Hanzel, PhDIn a series of three blog posts I will briefly explore the major cellular mechanisms responsible for many neurodegenerative disorders. The first, and perhaps the most apparent, is the accumulat...  Read full blog post.

Visualizing Autophagy by Microscopy
By Christina Towers, PhD Autophagy is a recycling process that relies on the formation of a unique organelle termed an autophagosome. An elegant way to monitor autophagy is through various microscopy techniques to vi...  Read full blog post.

The LC3 A, B, C’s and 1, 2, 3’s
By Christina Towers, PhD Autophagy is a catabolic process used to breakdown and recycle damaged proteins and organelles. It is a multistep process that, in its simplest form, consists of 4 steps: initiation, phagophor...  Read full blog post.

Best way to quantitatively measure Autophagic Flux
By Christina Towers, PhD Autophagy is a stress induced cellular recycling process that plays an important physiological role in many diseases. It is induced by a variety of stimuli both intracellular and extracellula...  Read full blog post.

Animal Models to Study Autophagy
By Christina Towers, PhD What is autophagy?Autophagy is the catabolic process that degrades cytoplasmic material via the lysosome. In the beginning... the process of macroautophagy was originally characterized in...  Read full blog post.

Best Methods to Induce and Inhibit Autophagy Pharmacologically
By Christina Towers, PhD Autophagy facilitates the degradation and recycling of damaged cytoplasmic material. The multistep process consists of a double membrane structure called an autophagosome that engulfs proteins...  Read full blog post.

Showing 1-10 of 52 blog posts - Show all blog posts.

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Bioinformatics

Gene Symbol BECN1