Western Blot: Beclin 1/ATG6 Antibody [NB110-87318] - Beclin 1 antibody image from verified customer review.
Immunocytochemistry/ Immunofluorescence: Beclin 1/ATG6 Antibody [NB110-87318] - Beclin1 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 ...read more
Immunohistochemistry: Beclin 1/ATG6 Antibody [NB110-87318] - Staining of breast carcinoma.
Western Blot: Beclin 1/ATG6 Antibody [NB110-87318] - Detection of Beclin 1 in HeLa whole cell lysate at 1:10,000.
Immunohistochemistry: Beclin 1/ATG6 Antibody [NB110-87318] - Staining of normal breast tissue.
Simple Western: Beclin 1/ATG6 Antibody [NB110-87318] - Simple Western lane view shows a specific band for Beclin in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS, 0.1% BSA, 50% glycerol
0.05% Sodium Azide
Immunogen affinity purified
Western Blot 1:10000
Simple Western 1:12.5
This Beclin 1 antibody is useful for Immunohistochemistry paraffin sections, Immunocytochemistry/Immunofluorescence, and Western blot where a band is seen ~51 kDa. In Simple Western only 10-15 uL of the recommended dilution is used per data point.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 3 Reviews rated 5 using NB110-87318 in the following applications:
Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumor suppressor and antiviral function. Autophagy, a process of bulk protein degradation through an autophagosomic-lysosomal pathway, is important for differentiation, survival during nutrient deprivation, and normal growth control, and is often defective in tumor cells. Beclin 1 was originally isolated in a yeast two hybrid screen to identify Bcl-2-binding partners and maps to a tumor susceptibility locus on human chromosome 17q21 that is frequently monoallelically deleted in human breast, ovarian and prostate cancer. Beclin 1 encodes an evolutionarily conserved 60 kDa coiled coil protein that is expressed in human muscle, epithelial cells and neurons. In gene transfer studies, Beclin 1 promotes nutrient deprivation-induced autophagy, inhibits mammary tumorigenesis, and inhibits viral replication. Expression of the Beclin 1 protein is frequently decreased in malignant breast epithelial cells. Based upon these observations, it is speculated that beclin 1 may work through induction of autophagy to negatively regulate tumorigenesis and to control viral infections. Beclin 1 may also play a role in other biological processes in which autophagy is important such as cell differentiation and nutritional stress responses.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for Beclin 1 Antibody (NB110-87318). (Showing 1 - 2 of 2 FAQs).
Can this NB110-87318 antibody applied to hamster?
The Beclin 1 Antibody, NB110-87318, has been validated in the following species: Human, mouse, rat, porcine, monkey, bovine, chicken, porcine and Xenopus. Hamster is not one of the species tested and validated and therefore we cannot guarantee that it will work in that species. If you are interested in testing this antibody in hamster, please take a look at our Innovators Reward Program.
We found the tested applications of NB110-87318 including IHC and IHC-P. Does it mean only IHC with paraffin embeded sections were tested with this antibody, and thus it may not work on frozen sections?
Our Beclin 1 antibody with catalogue number NB110-87318 has been validated for IHC with paraffin-embedded samples, but has not yet been tested with frozen tissue. We use the term IHC as a general search term, then this is further refined as IHC-P (paraffin-embedded) or IHC-Fr (frozen).
ATG9A - early marker autophagosome assembly ATG9A is the only essential integral membrane protein involved in autophagy. ATG9A contains six transmembrane domains and initiates the assembly of autophagosomes. The autophagosome is a double-membrane structure that engulfs and eventually degrade... Read full blog post.