Beclin 1 Antibody

Images

 
Western Blot: Beclin 1 Antibody [NB110-87318] - Total protein from human HeLa and mouse Neuro2A cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The ...read more
Immunocytochemistry/ Immunofluorescence: Beclin 1/ATG6 Antibody [NB110-87318] - Beclin1 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 ...read more
Immunohistochemistry-Paraffin: Beclin 1 Antibody [NB110-87318] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of human breast cancer using Beclin 1 antibody Lot F4 at 1:300 dilution. The ...read more
Western Blot: Beclin 1/ATG6 Antibody [NB110-87318] - Detection of Beclin 1 in HeLa whole cell lysate at 1:10,000.
Western Blot: Beclin 1/ATG6 Antibody [NB110-87318] - Beclin 1 antibody image from verified customer review.
Immunohistochemistry: Beclin 1/ATG6 Antibody [NB110-87318] - Staining of normal breast tissue.
Immunohistochemistry: Beclin 1/ATG6 Antibody [NB110-87318] - Staining of breast carcinoma.
Immunohistochemistry-Paraffin: Beclin 1 Antibody [NB110-87318] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of human breast cancer using Beclin 1 antibody Lot 71917 at 1:300 dilution. The ...read more
Simple Western: Beclin 1/ATG6 Antibody [NB110-87318] - Simple Western lane view shows a specific band for Beclin in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, Po, Ca, Pm, Rt, BvSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Beclin 1 Antibody Summary

Immunogen
Synthetic peptide made to an internal portion of human Beclin 1 (within residues 150-300). [Swiss-Prot# Q14457]
Localization
Cell Membrane, Cytoplasmic and Golgi Apparatus. Expressed in dendrites and cell bodies of cerebellar Purkinje cells
Predicted Species
Rat (96%), Bovine (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BECN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10000
  • Simple Western 1:12.5
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:50 - 1:400
  • Immunohistochemistry-Paraffin 1:50 - 1:400
Application Notes
This Beclin 1 antibody is useful for Immunohistochemistry paraffin sections, Immunocytochemistry/Immunofluorescence, and Western blot where a band is seen approx. 51 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Beclin 1 Lysate (NBL1-07966)
HeLa Lysate (NB820-59462)
Reviewed Applications
Read 4 Reviews rated 4.8
using
NB110-87318 in the following applications:

Publications
Read Publications using
NB110-87318 in the following applications:

  • 1 publication
  • IHC
    1 publication
  • WB
    15 publications

Reactivity Notes

Human, mouse, rat (96%), monkey, bovine (97%), porcine. Possible reactivity to Chicken (87%), Xenopus laevis (79%), and Xenopus tropicalis (77%) (not yet validated). Canine reactivity reported in scientific literature (PMID: 24027311).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 0.1% BSA, and 50% Glycerol
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Beclin 1 Antibody

  • ATG6 autophagy related 6 homolog
  • ATG6
  • beclin 1 (coiled-coil, moesin-like BCL2 interacting protein)
  • beclin 1 (coiled-coil, moesin-like BCL2-interacting protein)
  • Beclin 1
  • beclin 1, autophagy related
  • beclin1
  • beclin-1
  • BECN1
  • Coiled-coil myosin-like BCL2-interacting protein
  • GT197
  • Protein GT197
  • VPS30

Background

Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumor suppressor and antiviral function. Autophagy, a process of bulk protein degradation through an autophagosomic-lysosomal pathway, is important for differentiation, survival during nutrient deprivation, and normal growth control, and is often defective in tumor cells. Beclin 1 was originally isolated in a yeast two hybrid screen to identify Bcl-2-binding partners and maps to a tumor susceptibility locus on human chromosome 17q21 that is frequently monoallelically deleted in human breast, ovarian and prostate cancer. Beclin 1 encodes an evolutionarily conserved 60 kDa coiled coil protein that is expressed in human muscle, epithelial cells and neurons. In gene transfer studies, Beclin 1 promotes nutrient deprivation-induced autophagy, inhibits mammary tumorigenesis, and inhibits viral replication. Expression of the Beclin 1 protein is frequently decreased in malignant breast epithelial cells. Based upon these observations, it is speculated that beclin 1 may work through induction of autophagy to negatively regulate tumorigenesis and to control viral infections. Beclin 1 may also play a role in other biological processes in which autophagy is important such as cell differentiation and nutritional stress responses.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB110-87318
Species: Hu, Mu, Po, Ca, Pm, Rt, Bv
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P

Publications for Beclin 1 Antibody (NB110-87318)(27)

We have publications tested in 5 confirmed species: Human, Mouse, Canine, Porcine, Primate.

We have publications tested in 3 applications: ICC/IF, IHC, WB.


Filter By Application
ICC/IF
(1)
IHC
(1)
WB
(15)
All Applications
Filter By Species
Human
(8)
Mouse
(6)
Canine
(1)
Porcine
(2)
Primate
(1)
All Species
Showing Publications 1 - 10 of 27. Show All 27 Publications.
Publications using NB110-87318 Applications Species
Valera E, Spencer B, Mott J et al. MicroRNA-101 Modulates Autophagy and Oligodendroglial Alpha-Synuclein Accumulation in Multiple System Atrophy. Front Mol Neurosci. 2017 Nov 01 [PMID: 29089869] (WB, Human) WB Human
Mortezavi A, Salemi S, Rupp NJ et al. Negative LC3b immunoreactivity in cancer cells is an independent prognostic predictor of prostate cancer specific death. Oncotarget. 2017 May 09 [PMID: 28423666] (IHC, Human) IHC Human
Li Y, Wang F, Luo Y. Ginsenoside Rg1 protects against sepsis-associated encephalopathy through beclin 1-independent autophagy in mice. J Surg Res 2017 Jan [PMID: 27979475] (Mouse) Mouse
Tan X, Wang Hc, Liang Ry, Chuang Sm. Human Rad23A plays a regulatory role in autophagy. Biochem. Biophys. Res. Commun. Sep 30 2016 [PMID: 27613096]
Pei J, Deng J, Ye Z et al. Absence of autophagy promotes apoptosis by modulating the ROS-dependent RLR signaling pathway in classical swine fever virus-infected cells. Autophagy. Jul 27 2016 [PMID: 27463126] (WB, Porcine) WB Porcine
Choi MS, Kim HJ, Ham M et al. Amber Light (590 nm) Induces the Breakdown of Lipid Droplets through Autophagy-Related Lysosomal Degradation in Differentiated Adipocytes Sci Rep 2016 Jun 27 [PMID: 27346059]
Barnard RA, Regan DP, Hansen RJ et al. Autophagy inhibition delays early but not late stage metastatic disease. J. Pharmacol. Exp. Ther. May 26 2016 [PMID: 27231155] (WB, Mouse) WB Mouse
Song JX, Sun YR, Peluso I et al. A novel curcumin analog binds to and activates TFEB in vitro and in vivo independent of MTOR inhibition. Autophagy. May 12 2016 [PMID: 27172265] (WB, Mouse) WB Mouse
Lee YH, Chen HY, Su LJ, Chueh PJ. Sirtuin 1 (SIRT1) Deacetylase Activity and NAD(+)/NADH Ratio Are Imperative for Capsaicin-Mediated Programmed Cell Death. J. Agric. Food Chem. 2015 Aug 26 [PMID: 26255724] (WB, Human)

Details:
Beclin-1 antibody was use for WB on lysates of MRC-5 normal human foetal lung fibroblast cells and adenocarcinomic human alveolar basal epithelial cell line A549 that were treated or not with Capsaicin or ethanol.
WB Human
Chia-Wei Lin, Ming-Shiou Jan, Jung-Hua Steven Kuo. Exploring MicroRNA Expression Profiles Related to the mTOR Signaling Pathway in Mouse Embryonic Fibroblast Cells Treated with Polyethylenimine. Mol Pharm. 2015 Aug 3 [PMID: 26158199]
Show All 27 Publications.

Reviews for Beclin 1 Antibody (NB110-87318) (4) 4.84

Average Rating: 4.8
(Based on 4 reviews)
We have 4 reviews tested in 2 species: Human, Mouse.
We have 4 reviews tested in 1 application: WB.

Reviews using NB110-87318:
Filter by Applications
WB
(4)
All Applications
Filter by Species
Human
(3)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Mathumai Kanapathipillai
WB Human 03/03/2017
View

Summary

ApplicationWestern Blot
Sample TestedA549 human lung carcinoma cell line
SpeciesHuman
Western Blot Beclin 1 NB110-87318
Enlarge
reviewed by:
Anonymous
WB Human 04/25/2014
View

Summary

ApplicationWestern Blot
Sample TestedHuman microglia cell lysate
SpeciesHuman
LotD-1
Commentsthis antibody worked best for me at 1:5000 dilution however recommended dilution is 1:10000 so users can try other dilution if they are using different cell lysate

Blocking

Blocking Details5% BSA in 1X TBS 0.1%Tween20 for 1h at Room temperature

Primary Anitbody

Dilution Ratio1:5000 in 5% BSA in 1X TBS 0.1%Tween20 overnight incubation at 4 degree

Secondary Antibody

Secondary DescriptionAlk. Phosphtase conjugated anti rabbit
Secondary Manufacturer Cat#Cat #46-7006 from life technologies
Secondary Concentrationready to use 1X solution

Details

Detection NotesWesternBreeze Chemiluminescent Western Blot Immunodetection kit

Comments

Commentsthis antibody worked best for me at 1:5000 dilution however recommended dilution is 1:10000 so users can try other dilution if they are using different cell lysate
 
reviewed by:
Anonymous
WB Human 11/21/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman
SpeciesHuman
CommentsOk

Blocking

Blocking DetailsBlocking Buffer: 5% Milk

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab Dilution Ratio: 1:10000

Details

Detection NotesDetection Method: ECL-HRP

Comments

CommentsOk
 
reviewed by:
Anonymous
WB Mouse 11/21/2011
View

Summary

ApplicationWestern Blot
Sample TestedMouse
SpeciesMouse

Blocking

Blocking DetailsBlocking Buffer: 2% skim milk

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:10000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: Goat anti-rabbit HRP

Details

Detection NotesDetection Method: ECL

Product General Protocols

View specific protocols for Beclin 1 Antibody (NB110-87318): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Beclin 1 Antibody (NB110-87318). (Showing 1 - 2 of 2 FAQs).

  1. Can this NB110-87318 antibody applied to hamster?
    • The Beclin 1 Antibody, NB110-87318, has been validated in the following species: Human, mouse, rat, porcine, monkey, bovine, chicken, porcine and Xenopus. Hamster is not one of the species tested and validated and therefore we cannot guarantee that it will work in that species. If you are interested in testing this antibody in hamster, please take a look at our Innovators Reward Program.
  2. We found the tested applications of NB110-87318 including IHC and IHC-P. Does it mean only IHC with paraffin embeded sections were tested with this antibody, and thus it may not work on frozen sections?
    • Our Beclin 1 antibody with catalogue number NB110-87318 has been validated for IHC with paraffin-embedded samples, but has not yet been tested with frozen tissue. We use the term IHC as a general search term, then this is further refined as IHC-P (paraffin-embedded) or IHC-Fr (frozen).

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-87318AF405
Alexa Fluor 488 Labeled NB110-87318AF488
Alexa Fluor 647 Labeled NB110-87318AF647
Alexa Fluor 700 Labeled NB110-87318AF700
Biotin Labeled NB110-87318B
DyLight 488 Labeled NB110-87318G
DyLight 550 Labeled NB110-87318R
DyLight 650 Labeled NB110-87318C
HRP Labeled NB110-87318H

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Bioinformatics Tool for Beclin 1 Antibody (NB110-87318)

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Research Areas for Beclin 1 Antibody (NB110-87318)

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Blogs on Beclin 1.

Autophagy: Pro or Anti-tumorigenic? And the role of epigenetics in this debate
By Christina Towers, PhDAutophagy is an evolutionarily conserved process that cells use to break down damaged cytoplasmic constituents in order to fuel cellular metabolism, particularly in instances of stress. This process has been heavily...  Read full blog post.

Key Targets in Apoptosis, Necroptosis, and Autophagy
Cell death/recycling pathways such as apoptosis, necroptosis, and autophagy are an integral part of the growth, development, homeostasis as well as the pathophysiology in the life of living organisms. These signaling pathways are highly regulated and...  Read full blog post.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts...  Read full blog post.

UVRAG - A regulator of membrane trafficking in autophagy and endocytosis
UV resistance-associated gene (UVRAG) is a tumor suppressor that is commonly mutated in colon and breast cancer. While UVRAG was discovered for its ability to complement UV sensitivity in xeroderma pigmentosum cells, its main functions are in auto...  Read full blog post.

CHOP/GADD153 - A regulator and marker for ER-stress induced apoptosis
C/EBP homologous protein (CHOP) is a transcription factor that regulates apoptosis in response to cellular stress. CHOP also known as growth arrest and DNA damage 153 (GADD153) was first cloned because of its induction in response to genotoxic str...  Read full blog post.

Beclin 2, a mammal-specific homolog of Beclin 1 with unique functional similarities and differences
Beclin 2 (BECN2) is also called Beclin-1-like protein 1/ BECN1P1 and it was recently identified by He et al 2013 as a mammal-specific homolog of the evolutionarily conserved protein Beclin 1 which is well established for its role in the regulation...  Read full blog post.

Beclin 1 - a key regulator of autophagosome formation
The Beclin 1 protein is a central regulator of autophagy in mammalian cells. Autophagy is an essential process used to maintain cellular homeostasis by degrading and recycling cellular components such as damaged or worn out organelles and macromole...  Read full blog post.

ATG9A - early marker autophagosome assembly
ATG9A is the only essential integral membrane protein involved in autophagy. ATG9A contains six transmembrane domains and initiates the assembly of autophagosomes. The autophagosome is a double-membrane structure that engulfs and eventually degrade...  Read full blog post.

Wide Ranging Uses for the Autophagy Marker - Beclin-1 Antibody
Beclin 1 is the first mammalian gene identified as a mediator of autophagy, and plays important roles in development, tumorigenesis, and neurodegeneration. Research with the beclin-1 antibody has revealed that, Beclin 1 is found in complex with the th...  Read full blog post.

Beclin 1: Regulator of Autophagy and Apoptosis
Beclin 1 is the mammalian orthologue of the yeast Apg6/Vps30 gene. Beclin 1 can complement the defect in autophagy present in apg6 yeast strains and stimulate autophagy when overexpressed in mammalian cells (1) and can bind to Bcl2, an important regul...  Read full blog post.

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Recent Reviews

4.8
5
3
4
1
3
0
2
0
1
0

 
Mathumai Kanapathipillai
03/03/2017
Application: WB
Species: Human

Anonymous
04/25/2014
Application: WB
Species: Human

 
Anonymous
11/21/2011
Application: WB
Species: Human

Bioinformatics

Gene Symbol BECN1
Entrez