Beclin 1 Antibody - BSA Free


Simple Western: Beclin 1 Antibody [NB110-87318] - Lane view shows a specific band for Beclin in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Western Blot: Beclin 1 Antibody [NB110-87318] - Total protein from human HeLa and mouse Neuro2A cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The more
Immunohistochemistry-Paraffin: Beclin 1 Antibody [NB110-87318] - Analysis of a FFPE tissue section of human breast cancer using Beclin 1 antibody (Lot 71917) at 1:300 dilution. The primary antibody bound to Beclin 1 more
Western Blot: Beclin 1 Antibody [NB110-87318] - Basal levels of autophagy in the hypothalamus of BACHD mice. The expression levels of selected autophagy markers were analyzed by Western blot in hypothalamic tissue from more
Immunocytochemistry/ Immunofluorescence: Beclin 1 Antibody [NB110-87318] - Beclin 1/ATG6 Antibody [NB110-87318] - Beclin1 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained more
Immunohistochemistry-Paraffin: Beclin 1 Antibody [NB110-87318] - Analysis of a FFPE tissue section of human breast cancer using Beclin 1 antibody (Lot F4) at 1:300 dilution. The primary antibody bound to Beclin 1 more
Western Blot: Beclin 1 Antibody [NB110-87318] - Image from verified customer review.
Immunohistochemistry: Beclin 1/ATG6 Antibody [NB110-87318] - Staining of normal breast tissue.

Product Details

Reactivity Hu, Mu, Rt, Po, Al, Ca, Pm, Bv, Ch, Pm-OrSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, KD
BSA Free
1 mg/ml

Order Details

Beclin 1 Antibody - BSA Free Summary

Synthetic peptide made to an internal portion of human Beclin 1 (within residues 150-300). [Swiss-Prot: Q14457]
Cell Membrane, Cytoplasmic and Golgi Apparatus. Expressed in dendrites and cell bodies of cerebellar Purkinje cells
Predicted Species
Chicken (91%), Bovine (97%), Orangutan (99%). Backed by our 100% Guarantee.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:50 - 1:400
  • Immunohistochemistry-Paraffin 1:50 - 1:400
  • Knockdown Validated reported in scientific literature (PMID 29297744)
  • Simple Western 1:12.5
  • Western Blot 1:1000
Application Notes
In Western blot, a band is seen approx. 51 kDa. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
Theoretical MW
51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Beclin 1 Overexpression Lysate
Reviewed Applications
Read 4 Reviews rated 4.8
NB110-87318 in the following applications:

Read Publications using
NB110-87318 in the following applications:

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34622072). Canine reactivity reported in scientific literature (PMID: 24027311). Use in Rat reported in scientific literature (PMID:32501746).

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS, 0.02 Sodium Azide
0.02% Sodium Azide
1 mg/ml
Immunogen affinity purified

Alternate Names for Beclin 1 Antibody - BSA Free

  • ATG6 autophagy related 6 homolog
  • ATG6
  • beclin 1 (coiled-coil, moesin-like BCL2 interacting protein)
  • beclin 1 (coiled-coil, moesin-like BCL2-interacting protein)
  • Beclin 1
  • beclin 1, autophagy related
  • beclin1
  • beclin-1
  • BECN1
  • Coiled-coil myosin-like BCL2-interacting protein
  • GT197
  • Protein GT197
  • VPS30


Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumor suppressor and antiviral function. Autophagy, a process of bulk protein degradation through an autophagosomic-lysosomal pathway, is important for differentiation, survival during nutrient deprivation, and normal growth control, and is often defective in tumor cells. Beclin 1 was originally isolated in a yeast two hybrid screen to identify Bcl-2-binding partners and maps to a tumor susceptibility locus on human chromosome 17q21 that is frequently monoallelically deleted in human breast, ovarian and prostate cancer. Beclin 1 encodes an evolutionarily conserved 60 kDa coiled coil protein that is expressed in human muscle, epithelial cells and neurons. In gene transfer studies, Beclin 1 promotes nutrient deprivation-induced autophagy, inhibits mammary tumorigenesis, and inhibits viral replication. Expression of the Beclin 1 protein is frequently decreased in malignant breast epithelial cells. Based upon these observations, it is speculated that beclin 1 may work through induction of autophagy to negatively regulate tumorigenesis and to control viral infections. Beclin 1 may also play a role in other biological processes in which autophagy is important such as cell differentiation and nutritional stress responses.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Am, Ca, Fi, Hu, Mu, Pl, Rt, Ze
Applications: ChIP, ChIP, ELISA, Flow, IB, ICC/IF, IHC-WhMt, IHC, IHC-Fr, IHC-P, IP, Simple Western, SB, WB
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
Species: Al, Bv, Dr, Fi, Gp, Hu, Mu, Po, Pm, Rt, Xp, Ze
Applications: EM, ELISA, Flow, IB, ICC/IF, IHC, IHC-P, IP, KD, KO, PLA, RIA, Simple Western, WB
Species: Hu, Rt
Applications: ELISA, IHC, IHC-P, NULL, WB
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-Fr, IHC-P, IP, WB
Species: Bv, Ha, Hu, Mu, Rb, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
Species: Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow, Simple Western, WB
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
Species: Hu, Mu, Po, Rt
Applications: ICC/IF, KD, Simple Western, WB
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, KD, S-ELISA, WB
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, WB
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, WB
Species: Ca, Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
Species: Bv, Hu, Ma
Applications: ICC/IF, IHC, IHC-P, IP, WB
Species: Bv, Hu, Pm, Mu, Po, Rt
Applications: Func, IHC, IHC-Fr, IHC-P, WB
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KO, WB
Species: Hu, Mu, Rt, Po, Al, Ca, Pm, Bv, Ch, Pm-Or
Applications: WB, Simple Western, ICC/IF, IHC, KD

Publications for Beclin 1 Antibody (NB110-87318)(54)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Alligator, Canine, Porcine, Primate.

We have publications tested in 4 applications: ICC/IF, IF/IHC, KD, WB.

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Showing Publications 1 - 10 of 54. Show All 54 Publications.
Publications using NB110-87318 Applications Species
Wen Y, Chen Z, Tian Y et al. Incomplete autophagy promotes the proliferation of Mycoplasma hyopneumoniae through the JNK and Akt pathways in porcine alveolar macrophages Veterinary research 2022-08-04 [PMID: 35927699] (WB, Porcine) WB Porcine
Izumi H, Li Y, Yasunami M et al. Asymmetric Pericentrosomal CD133 Endosomes Induce the Unequal Autophagic Activity During Cytokinesis in CD133-Positive Human Neuroblastoma Cells Stem cells (Dayton, Ohio) 2022-03-14 [PMID: 35284915]
Chen y, Chen S, Li T et al. 4-Acetylantroquinonol B enhances cell death and inhibits autophagy by downregulating the PI3K/Akt/MDR1 pathway in gemcitabine-resistant pancreatic cancer cells Oncology Letters 2022-02-18 [PMID: 35251348]
Wang yT, Liu Ty, Shen CH et al. K48/K63-linked polyubiquitination of ATG9A by TRAF6 E3 ligase regulates oxidative stress-induced autophagy Cell reports 2022-02-22 [PMID: 35196483] (WB, Mouse, Human) WB Mouse, Human
Huang PJ, Chiu CC, Hsiao MH et al. Potential of antiviral drug oseltamivir for the treatment of liver cancer International journal of oncology 2021-12-01 [PMID: 34859259] (WB, Human) WB Human
Wang CC, Lin SY, Huang YH Et al. Paired-like homeodomain 2B contributes to tumour progression and anti-autophagy in human lung cancer American journal of cancer research 2021-10-15 [PMID: 34765299] (WB, Human) WB Human
Zhang X, Xiao R, Lu B Et al. Kinase DYRK2 acts as a regulator of autophagy and an indicator of favorable prognosis in gastric carcinoma Colloids and Surfaces B: Biointerfaces 2021-10-01 [PMID: 34749023] (WB, Human) WB Human
Parry H, Randall R, Hyatt H et al. Short and long-term effect of reproduction on mitochondrial dynamics and autophagy in rats Heliyon 2021-09-01 [PMID: 34622072] (WB, Rat) WB Rat
Wang Z, Wen Y, Zhou B et al. Incomplete autophagy promotes the replication of Mycoplasma hyopneumoniae Journal of microbiology (Seoul, Korea) 2021-08-01 [PMID: 34219210]
Ilnytska O, Lai K, Gorshkov K et al. Enrichment of NPC1-deficient cells with the lipid LBPA stimulates autophagy, improves lysosomal function, and reduces cholesterol storage The Journal of biological chemistry 2021-05-20 [PMID: 34023384]
Show All 54 Publications.

Reviews for Beclin 1 Antibody (NB110-87318) (4) 4.84

Average Rating: 4.8
(Based on 4 reviews)
We have 4 reviews tested in 2 species: Human, Mouse.

Reviews using NB110-87318:
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Images Ratings Applications Species Date Details
reviewed by:
Verified Customer
WB Human 03/03/2017


ApplicationWestern Blot
Sample TestedA549 human lung carcinoma cell line
Western Blot Beclin 1 NB110-87318
reviewed by:
Pratima Rawat
WB Human 04/25/2014


ApplicationWestern Blot
Sample TestedHuman microglia cell lysate
reviewed by:
Ming-Tsang Chiao
WB Human 11/21/2011


ApplicationWestern Blot
Sample TestedHuman
reviewed by:
Ming-Shiou Jan
WB Mouse 11/21/2011


ApplicationWestern Blot
Sample TestedMouse

Product General Protocols

View specific protocols for Beclin 1 Antibody (NB110-87318): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Beclin 1 Antibody (NB110-87318). (Showing 1 - 2 of 2 FAQs).

  1. Can this NB110-87318 antibody applied to hamster?
    • The Beclin 1 Antibody, NB110-87318, has been validated in the following species: Human, mouse, rat, porcine, monkey, bovine, chicken, porcine and Xenopus. Hamster is not one of the species tested and validated and therefore we cannot guarantee that it will work in that species. If you are interested in testing this antibody in hamster, please take a look at our Innovators Reward Program.
  2. We found the tested applications of NB110-87318 including IHC and IHC-P. Does it mean only IHC with paraffin embeded sections were tested with this antibody, and thus it may not work on frozen sections?
    • Our Beclin 1 antibody with catalogue number NB110-87318 has been validated for IHC with paraffin-embedded samples, but has not yet been tested with frozen tissue. We use the term IHC as a general search term, then this is further refined as IHC-P (paraffin-embedded) or IHC-Fr (frozen).

Control Lysate(s)

Secondary Antibodies


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Blogs on Beclin 1. Showing 1-10 of 16 blog posts - Show all blog posts.

Autophagy and RAS signaling: Clinical implications
By Christina Towers, PhD The cellular recycling process known as autophagy is currently being targeted in over 60 clinical trials focused on treating different types of cancer1. To date, the only autophagy-targeted ...  Read full blog post.

Best Methods to Induce and Inhibit Autophagy Pharmacologically
By Christina Towers, PhD Autophagy facilitates the degradation and recycling of damaged cytoplasmic material. The multistep process includes a double membrane structure called an autophagosome that engulfs pr...  Read full blog post.

E-syt in Autophagosome biogenesis: What is the source of it all?
By Christina Towers, PhD. Macroautophagy is a cellular recycling process that requires the formation of double membrane structures to engulf and degrade damaged cytoplasmic material. The pathway involves over 20 co...  Read full blog post.

Lysosomal Dysfunction is Linked to Exosomal Secretion
By Christina Towers, PhD. Lysosomal Dysfunction and DiseaseLysosomes are highly acidic organelles that are critical for cellular function and indispensable for degradative pathways like autophagy and endocytosis....  Read full blog post.

CaMKII stimulates autophagic degradation of 'ID', a new frontier against cancer
By Yoskaly Lazo-Fernandez, PhD The field of Cancer Stem Cell (CSC) research has been gaining traction in recent years1. CSCs are a minority group of cells (usually about 1 in 10000) within solid tumors of hematolog...  Read full blog post.

Brain size matters: MTOR regulates autophagy and number of cortical interneurons
By Jamshed Arslan Pharm.D. Interneurons transmit impulses between other neurons, in part, to facilitate the birth of neurons. Cortical interneurons themselves arise from the progenitors in the ventral telencephalo...  Read full blog post.

Autophagy: Pro or Anti-tumorigenic? And the role of epigenetics in this debate
By Christina Towers, PhDAutophagy is an evolutionarily conserved process that cells use to break down damaged cytoplasmic constituents in order to fuel cellular metabolism, particularly in instances of stress. This process has been heavily ...  Read full blog post.

Key Targets in Apoptosis, Necroptosis, and Autophagy
Cell death/recycling pathways such as apoptosis, necroptosis, and autophagy are an integral part of the growth, development, homeostasis as well as the pathophysiology in the life of living organisms. These signaling pathways are highly regulated and ...  Read full blog post.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts...  Read full blog post.

UVRAG - A regulator of membrane trafficking in autophagy and endocytosis
UV resistance-associated gene (UVRAG) is a tumor suppressor that is commonly mutated in colon and breast cancer. While UVRAG was discovered for its ability to complement UV sensitivity in xeroderma pigmentosum cells, its main functions are in auto...  Read full blog post.

Showing 1-10 of 16 blog posts - Show all blog posts.
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Recent Reviews


Verified Customer
Application: WB
Species: Human

Pratima Rawat
Application: WB
Species: Human

Ming-Tsang Chiao
Application: WB
Species: Human


Gene Symbol BECN1