VPS34 Antibody

Images

 
Western Blot: VPS34 Antibody [NB110-87320] - HepG2 whole cell lysates.
Immunocytochemistry/ Immunofluorescence: VPS34 Antibody [NB110-87320] - VPS34 antibody was tested at 1:200 in HeLa cells with FITC (green). Nuclei (Blue) were counterstained with Dapi (blue).
Simple Western: VPS34 Antibody [NB110-87320] - Simple Western lane view shows a specific band for VPS34 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

VPS34 Antibody Summary

Immunogen
Synthetic peptide made to an internal portion of the human VPS34 protein (within residues 700-850). [Swiss-Prot# Q8NEB9]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PIK3C3
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:20
  • Immunocytochemistry/Immunofluorescence 1:100
Application Notes
This VPS34 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen approx. 100 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
VPS34 Lysate (NBL1-14416)
Reviewed Applications
Read 1 Review rated 4
using
NB110-87320 in the following applications:

Publications
Read Publications using
NB110-87320 in the following applications:

  • WB
    4 publications

Reactivity Notes

Human, rat, mouse and porcine. Immunogen sequence has 87% homology to Xenopus.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 0.1% BSA, and 50% Glycerol
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for VPS34 Antibody

  • EC 2.7.1
  • EC 2.7.1.137
  • hVps34
  • MGC61518
  • phosphatidylinositol 3-kinase catalytic subunit type 3
  • Phosphatidylinositol 3-kinase p100 subunit
  • Phosphoinositide-3-kinase class 3
  • phosphoinositide-3-kinase, class 3
  • PI 3-Kinase C3
  • PI3K type 3
  • PI3-kinase type 3
  • PIK3C3
  • PtdIns-3-kinase type 3
  • VPS34

Background

VPS34 (vacuolar protein sorting 34) is a member of PI3K family of lipid kinases, all of which phosphorylate the 3' hydroxy position of phosphatidylinositol ring. VPS34 is ubiquitously expressed with highest expression in skeletal muscles and it localizes to midbody /late endosomes in cells. VPS34 forms a complexes with BECN1, PIK3R4 and either UVRAG and KIAA0226/Rubicon, or with ATG14, and the presence of UVRAG/ATG14 are mutually exclusive in this complex. It forms complex with PIK3R4/PIK3CB also, and interacts with RAB7A in the presence of PIK3R4. VPS34 regulate vesicular trafficking in the endosomal/lysosomal system, where it is involved in the recruitment of proteins containing phosphatidylinositol 3-phosphate (PtdIns3P) binding domains to intracellular membranes. As PI3K complex's catalytic subunit, VPS34 mediates formation of PtdIns3P which is involved in initiation and maturation of autophagosomes. Moreover, VPS34 is implicated in transporting lysosomal enzyme precursors to lysosomes, required for abcission step in cytokinesis and transport from early to late endosomes. Vps34 has also been involved in other signalling processes such as nutrient sensing in mTOR pathway in mammalian cells and trimeric G-protein signalling to MAPK in yeast.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VPS34 Antibody (NB110-87320)(4)

Review for VPS34 Antibody (NB110-87320) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB110-87320:
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WB
(1)
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(1)
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Images Ratings Applications Species Date Details
Western Blot VPS34 NB110-87320
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reviewed by:
Naoya Nakai
WB Mouse 07/27/2017
View

Summary

ApplicationWestern Blot
Sample TestedSkeletal muscle satellite cells (cultured in vitro)
SpeciesMouse

Product General Protocols

View specific protocols for VPS34 Antibody (NB110-87320): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for VPS34 Antibody (NB110-87320) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on VPS34.

E-syt in Autophagosome biogenesis: What is the source of it all?
By Christina Towers, PhD. Macroautophagy is a cellular recycling process that requires the formation of double membrane structures to engulf and degrade damaged cytoplasmic material. The pathway involves over 20 co...  Read full blog post.

Lysosomal Dysfunction is Linked to Exosomal Secretion
By Christina Towers, PhD. Lysosomal Dysfunction and DiseaseLysosomes are highly acidic organelles that are critical for cellular function and indispensable for degradative pathways like autophagy and endocytosis....  Read full blog post.

UVRAG - A regulator of membrane trafficking in autophagy and endocytosis
UV resistance-associated gene (UVRAG) is a tumor suppressor that is commonly mutated in colon and breast cancer. While UVRAG was discovered for its ability to complement UV sensitivity in xeroderma pigmentosum cells, its main functions are in auto...  Read full blog post.

VPS34 - autophagy initiator and regulator of endosomal trafficking
VPS34, vacuolar protein sorting 34, is the only identified Class III phosphoinositide-3 kinase (PI3K) in mammals and is ubiquitously expressed in all eukaryotic cells. VPS34 is a 100 kDa protein responsible for phosphorylating phosphatidylinositol...  Read full blog post.

ULK1 - mammalian homologue of the yeast ATG1 kinase
Autophagy is an important cellular process involved in degradation and recycling of cellular macromolecules in response to stress or starvation. Autophagy is carried out in four main phases: phagophore nucleation, autophagosome elongation, docking...  Read full blog post.

VPS41 - An important regulator of lysosomal trafficking
Membrane fusion is an essential step during the trafficking of endosomes and vesicles throughout the cell. Membrane fusion events are facilitated by multisubunit tethering complexes (MTC) including CORVET and HOPS. These complexes interact with Rab...  Read full blog post.

Beclin 2, a mammal-specific homolog of Beclin 1 with unique functional similarities and differences
Beclin 2 (BECN2) is also called Beclin-1-like protein 1/ BECN1P1 and it was recently identified by He et al 2013 as a mammal-specific homolog of the evolutionarily conserved protein Beclin 1 which is well established for its role in the regulation...  Read full blog post.

Beclin 1 - a key regulator of autophagosome formation
The Beclin 1 protein is a central regulator of autophagy in mammalian cells. Autophagy is an essential process used to maintain cellular homeostasis by degrading and recycling cellular components such as damaged or worn out organelles and macromole...  Read full blog post.

ATG9A - early marker autophagosome assembly
ATG9A is the only essential integral membrane protein involved in autophagy. ATG9A contains six transmembrane domains and initiates the assembly of autophagosomes. The autophagosome is a double-membrane structure that engulfs and eventually degrade...  Read full blog post.

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Recent Reviews

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5
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4
1
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2
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1
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Naoya Nakai
07/27/2017
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol PIK3C3
Entrez
Uniprot