VPS34 Antibody


Simple Western: VPS34 Antibody [NB110-87320] - Simple Western lane view shows a specific band for VPS34 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Genetic Strategies: Knockdown Validated: VPS34 Antibody [NB110-87320] - ATG7 depleted SKBR3 cells show increased sensitivity to ATRA and the RARalpha agonist AM580. RARalpha , ATG5 and VPS34 Western blot ...read more
Western Blot: VPS34 Antibody [NB110-87320] - HepG2 whole cell lysates.
Immunocytochemistry/ Immunofluorescence: VPS34 Antibody [NB110-87320] - VPS34 antibody was tested at 1:200 in HeLa cells with FITC (green). Nuclei (Blue) were counterstained with Dapi (blue).

Product Details

Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications WB, Simple Western, ICC/IF, KD
0.2 mg/ml
Validated by:

Genetic Strategies


Order Details

VPS34 Antibody Summary

Synthetic peptide made to an internal portion of the human VPS34 protein (within residues 700-850). [Swiss-Prot# Q8NEB9]
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunocytochemistry/Immunofluorescence 1:100
  • Knockdown Validated
  • Simple Western 1:20
  • Western Blot 1:1000
Application Notes
This VPS34 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen approx. 100 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
VPS34 Overexpression Lysate
Reviewed Applications
Read 1 Review rated 4
NB110-87320 in the following applications:

Read Publications using
NB110-87320 in the following applications:

  • 1 publication
  • WB
    6 publications

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus (87%).

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS, 0.1% BSA, and 50% Glycerol
0.05% Sodium Azide
0.2 mg/ml
Immunogen affinity purified

Alternate Names for VPS34 Antibody

  • EC 2.7.1
  • EC
  • hVps34
  • MGC61518
  • phosphatidylinositol 3-kinase catalytic subunit type 3
  • Phosphatidylinositol 3-kinase p100 subunit
  • Phosphoinositide-3-kinase class 3
  • phosphoinositide-3-kinase, class 3
  • PI 3-Kinase C3
  • PI3K type 3
  • PI3-kinase type 3
  • PIK3C3
  • PtdIns-3-kinase type 3
  • VPS34


VPS34 (vacuolar protein sorting 34) is a member of PI3K family of lipid kinases, all of which phosphorylate the 3' hydroxy position of phosphatidylinositol ring. VPS34 is ubiquitously expressed with highest expression in skeletal muscles and it localizes to midbody/late endosomes in cells. VPS34 forms a complexes with BECN1, PIK3R4 and either UVRAG and KIAA0226/Rubicon, or with ATG14, and the presence of UVRAG/ATG14 are mutually exclusive in this complex. It forms complex with PIK3R4/PIK3CB also, and interacts with RAB7A in the presence of PIK3R4. VPS34 regulates vesicular trafficking in the endosomal/lysosomal system, where it is involved in the recruitment of proteins containing phosphatidylinositol 3-phosphate (PtdIns3P) binding domains to intracellular membranes. As PI3K complex's catalytic subunit, VPS34 mediates formation of PtdIns3P which is involved in initiation and maturation of autophagosomes. Moreover, VPS34 is implicated in transporting lysosomal enzyme precursors to lysosomes, required for abcission step in cytokinesis and transport from early to late endosomes. Vps34 has also been involved in other signalling processes such as nutrient sensing in mTOR pathway in mammalian cells and trimeric G-protein signalling to MAPK in yeast.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VPS34 Antibody (NB110-87320)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: FLOW, WB.

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Showing Publications 1 - 8 of 8.
Publications using NB110-87320 Applications Species
Yang D, Wu X, Wang W et al. Ciliary Type III Adenylyl Cyclase in the VMH Is Crucial for High-Fat Diet-Induced Obesity Mediated by Autophagy Advanced Science 2022-01-01 [PMID: 34783461] (WB) WB
Rijal R, Cadena LA, Smith MR et al. Polyphosphate is an extracellular signal that can facilitate bacterial survival in eukaryotic cells Proceedings of the National Academy of Sciences of the United States of America 2020-12-15 [PMID: 33268492]
Meena N, Ralston E, Raben N, Puertollano R Enzyme Replacement Therapy Can Reverse Pathogenic Cascade in Pompe Disease Mol Ther Methods Clin Dev 2020-07-17 [PMID: 32671132] (FLOW, Human) FLOW Human
Lin M W, Lin C C et al. Celastrol Inhibits Dopaminergic Neuronal Death of Parkinson's Disease through Activating Mitophagy. Antioxidants (Basel) 2019-12-31 [PMID: 31906147] (WB, Mouse) WB Mouse
Campa CC, Margaria JP, Derle A et al. Rab11 activity and PtdIns(3)P turnover removes recycling cargo from endosomes Nat. Chem. Biol. 2018-06-18 [PMID: 29915378] (WB) WB
Brigger D, Schlafli AM, Garattini E, Tschan MP. Activation of RARa induces autophagy in SKBR3 breast cancer cells and depletion of key autophagy genes enhances ATRA toxicity. Cell Death Dis 2015-08-28 [PMID: 26313912] (WB, Human) WB Human
Shi JM, Bai LL, Zhang DM et al. Saxifragifolin D induces the interplay between apoptosis and autophagy in breast cancer cells through ROS-dependent endoplasmic reticulum stress. Biochem Pharmacol 2013-01-21 [PMID: 23348250] (WB, Human) WB Human
Huang Y, Hou JK, Chen TT et al. PML-RAR alpha enhances constitutive autophagic activity through inhibiting the Akt/mTOR pathway. Autophagy. 2011-10-01 [PMID: 21673516] (WB, Human) WB Human

Review for VPS34 Antibody (NB110-87320) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB110-87320:
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Images Ratings Applications Species Date Details
Western Blot VPS34 NB110-87320
reviewed by:
Naoya Nakai
WB Mouse 07/27/2017


ApplicationWestern Blot
Sample TestedSkeletal muscle satellite cells (cultured in vitro)

Product General Protocols

View specific protocols for VPS34 Antibody (NB110-87320): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for VPS34 Antibody (NB110-87320) (0)

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Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Blogs on VPS34. Showing 1-10 of 12 blog posts - Show all blog posts.

Autophagy Research Update: What a difference a year makes!
By Christina Towers, PhD Over the last two decades the field of autophagy has exploded! Innovative techniques, comprehensive analysis and disease-relevant models have yielded basic and clinical discoveries of conseque...  Read full blog post.

Animal Models to Study Autophagy
By Christina Towers, PhD What is autophagy?Autophagy is the catabolic process that degrades cytoplasmic material via the lysosome. The process of macroautophagy was originally characterized in yeast, where the...  Read full blog post.

Best Methods to Induce and Inhibit Autophagy Pharmacologically
By Christina Towers, PhD Autophagy facilitates the degradation and recycling of damaged cytoplasmic material. The multistep process includes a double membrane structure called an autophagosome that engulfs pr...  Read full blog post.

E-syt in Autophagosome biogenesis: What is the source of it all?
By Christina Towers, PhD. Macroautophagy is a cellular recycling process that requires the formation of double membrane structures to engulf and degrade damaged cytoplasmic material. The pathway involves over 20 co...  Read full blog post.

Lysosomal Dysfunction is Linked to Exosomal Secretion
By Christina Towers, PhD. Lysosomal Dysfunction and DiseaseLysosomes are highly acidic organelles that are critical for cellular function and indispensable for degradative pathways like autophagy and endocytosis....  Read full blog post.

UVRAG - A regulator of membrane trafficking in autophagy and endocytosis
UV resistance-associated gene (UVRAG) is a tumor suppressor that is commonly mutated in colon and breast cancer. While UVRAG was discovered for its ability to complement UV sensitivity in xeroderma pigmentosum cells, its main functions are in auto...  Read full blog post.

VPS34 - autophagy initiator and regulator of endosomal trafficking
VPS34, vacuolar protein sorting 34, is the only identified Class III phosphoinositide-3 kinase (PI3K) in mammals and is ubiquitously expressed in all eukaryotic cells. VPS34 is a 100 kDa protein responsible for phosphorylating phosphatidylinositol...  Read full blog post.

ULK1 - mammalian homologue of the yeast ATG1 kinase
Autophagy is an important cellular process involved in degradation and recycling of cellular macromolecules in response to stress or starvation. Autophagy is carried out in four main phases: phagophore nucleation, autophagosome elongation, docking...  Read full blog post.

VPS41 - An important regulator of lysosomal trafficking
Membrane fusion is an essential step during the trafficking of endosomes and vesicles throughout the cell. Membrane fusion events are facilitated by multisubunit tethering complexes (MTC) including CORVET and HOPS. These complexes interact with Rab...  Read full blog post.

Beclin 2, a mammal-specific homolog of Beclin 1 with unique functional similarities and differences
Beclin 2 (BECN2) is also called Beclin-1-like protein 1/ BECN1P1 and it was recently identified by He et al 2013 as a mammal-specific homolog of the evolutionarily conserved protein Beclin 1 which is well established for its role in the regulation ...  Read full blog post.

Showing 1-10 of 12 blog posts - Show all blog posts.
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Recent Reviews


Naoya Nakai
Application: WB
Species: Mouse


Gene Symbol PIK3C3