ATG16L1 Antibody

Images

 
Western Blot: ATG16L1 Antibody [NB110-82384] - Analysis of ATG16L1 in HeLa whole cell extracts.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.65 mg/ml

ATG16L1 Antibody Summary

Immunogen
A synthetic peptide within residues 1-100 of mouse ATG16L1. [Swiss-Prot# Q8C0J2]
Localization
Cytoplasm. Preautophagosomal structure, preautophagosomal structure membrane, peripheral membrane protein. Note: Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATG16L1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Immunoprecipitation
Application Notes
This ATG16L1 antibody is useful for Western blot, where a band can be seen ~66 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in immunoprecipitation reported in scientific literature (PMID: 29361549).
Theoretical MW
66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
NIH 3T3 Whole Cell Lysate
Control Peptide
ATG16L1 Peptide (NB110-82384PEP)
Publications
Read Publication using NB110-82384.

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
0.65 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ATG16L1 Antibody

  • APG16 autophagy 16-like (S. cerevisiae)
  • APG16L beta
  • APG16LFLJ10828
  • APG16-like 1
  • ATG16 autophagy related 16-like (S. cerevisiae)
  • ATG16 autophagy related 16-like 1 (S. cerevisiae)
  • ATG16A
  • ATG16L
  • autophagy-related protein 16-1
  • FLJ00045
  • FLJ10035
  • FLJ22677
  • IBD10
  • WD repeat domain 30
  • WDR30

Background

Atg16L1 (autophagy-related protein 16-1) forms a component of a protein complex that directs LC3 to autophagosomes en route to their fusion with lysosomes, thereby playing a key role in the process of autophagosome maturation. Atg16L1 belongs to WD repeat ATG16 family, exist as homooligomer and interacts with ATG5 as well as RAB33B, and forms a part of either the minor and major complexes respectively composed of 4 sets of ATG12-ATG5 and ATG16L1 (400 kD) or 8 sets of ATG12-ATG5 and ATG16L1 (800 kD). It is found in cytoplasm and gets localized to preautophagosomal structure where it is implicated in the membrane targeting of ATG5. Mice deficient in Atg16L1 (Atg16L1HM) display reduced autophagy and develop, on viral infection, intestinal abnormalities similar to pathologies found in Crohn's disease patients, including abnormalities in the integrity, architecture, and function of Paneth cells, which are specialized secretory epithelial cells of the small intestine. Atg16L1 has also been shown to play a role in modulating proinflammatory responses and population genetic studies have positively linked a common polymorphism in ATG16L1 gene with inflammatory bowel disease type 10 (IBD10).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, IP

Publications for ATG16L1 Antibody (NB110-82384)(1)


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Product General Protocols

View specific protocols for ATG16L1 Antibody (NB110-82384): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB110-82384AF350
Alexa Fluor 405 NB110-82384AF405
Alexa Fluor 488 NB110-82384AF488
Alexa Fluor 532 NB110-82384AF532
Alexa Fluor 594 NB110-82384AF594
Alexa Fluor 647 NB110-82384AF647
Alexa Fluor 700 NB110-82384AF700
Alexa Fluor 750 NB110-82384AF750
Biotin NB110-82384B
DyLight 350 NB110-82384UV
DyLight 405 NB110-82384V
DyLight 488 NB110-82384G
DyLight 550 NB110-82384R
DyLight 594 NB110-82384DL594
DyLight 650 NB110-82384C
DyLight 680 NB110-82384FR
DyLight 755 NB110-82384IR
HRP NB110-82384H

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Blogs on ATG16L1.

Liver ASK1 activates autophagy to protect against hepatic fat accumulation, non-alcoholic steatohepatitis and fibrosis
By Jamshed Arslan, Pharm. D., PhD. The most common chronic liver disorder worldwide is non-alcoholic fatty liver disease (NAFLD). This obesity-linked disorder can manifest as hepatic fat accumulation (steatosis) wit...  Read full blog post.

ATG16L1 - a key player in the development of the autophagosome
Like apoptosis, autophagy is a highly regulated physiologic process that involves cellular degradation and recycling of organelles and macromolecules. Autophagy is a survival mechanism induced by states of stress, starvation, and infection. A do...  Read full blog post.

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Bioinformatics

Gene Symbol ATG16L1