ATG9A Antibody


Western Blot: ATG9A Antibody [NB110-56893] - Detection of ATG9A protein in HEK293 lysates using NB110-56893. Lane 1: siRNA ATG9A knockdown Lane 2: wildtype ATG9A.
Immunohistochemistry: ATG9A Antibody [NB110-56893] - Staining in mouse intestine.

Product Details

Reactivity Hu, Mu, Rt, Bv, Ch, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
1 mg/ml

ATG9A Antibody Summary

A synthetic peptide made to a region within the C-terminus of human ATG9A (between residues 750-839). [Swiss-Prot# Q7Z3C6].
Membrane; multi-pass membrane protein.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
PBS, 30% glycerol
0.1% Sodium Azide
1 mg/ml
Immunogen affinity purified


Application Notes
This ATG9A antibody is useful for Immunohistochemistry paraffin embedded sections and Western blot. In Western blot this antibody recognizes a band at ~95 kDa, representing ATG9A protein. Boiling samples prior to running the gel may affect the protein. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 24705551) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
95 kDa
Control Peptide
ATG9A Peptide (NB110-56893PEP)
Reviewed Applications
Read 1 Review rated 5
NB110-56893 in the following application:

Read Publications using
NB110-56893 in the following applications:

  • 1 publication
  • IHC
    2 publications
  • IP
    2 publications
  • WB
    7 publications

Alternate Names for ATG9A Antibody

  • APG9 autophagy 9-like 1 (S. cerevisiae)
  • APG9 autophagy 9-like 1
  • APG9L1APG9-like 1
  • ATG9 autophagy related 9 homolog A (S. cerevisiae)
  • autophagy 9-like 1 protein
  • autophagy-related protein 9A
  • FLJ22169
  • mATG9
  • MGD3208


ATG9A (autophagy-related protein 9A) is the only transmembrane member identified among the classical ATG proteins family which are essential to autophagy. Yeast ATG9 protein localizes to the pre-autophagosomal structure (PAS) as well as cytoplasmic punctate structures, whereas, the mammalian ATG9A localizes to endocytic compartments, the trans-Golgi network and autophagic membranes. Moreover, ATG9 is recruited to the PAS in an ATG11-dependent manner under nutrient-rich conditions, whereas during starvation, recruitment of ATG9 depends on Atg17p. ATG9A does not steadily reside at one place in cells, but rather dynamically shuttles between a juxta-nuclear trans-Golgi network compartment and late endosomes under autophagic or starvation conditions. Moreover, ATG9A accumulation is upregulated upon starvation in autophagosomes and the starvation-dependent trafficking requires ULK1, ATG13 and SUPT20H. ATG9A interacts with SUPT20H and has been proposed to mediate membrane transport to generate autophagosomes.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Pm
Applications: WB, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IF
Species: Hu, Mu, Rt, Ca, Fi, Ze
Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, SB
Species: Hu, Mu, Rt, Bv, Ca
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Mk
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Bv, Ca, Pm
Applications: WB, EM, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu
Applications: WB, IHC-P
Species: Hu
Applications: WB, IHC, ICC
Species: Hu
Applications: WB, IHC
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Po, Bv, Pm, Xp, Ze
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, IP, PLA
Species: Hu, Mu, Rt, Ca, Ch, Pm
Applications: WB
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Rt, Pm
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, IP
Species: Hu, Mu, Rt, Bv, Ch, Pm
Applications: WB, ICC/IF, IHC, IHC-P

Publications for ATG9A Antibody (NB110-56893)(12)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Drosophila.

We have publications tested in 4 applications: ICC/IF, IHC, IP, WB.

Filter By Application
All Applications
Filter By Species
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NB110-56893 Applications Species
Staudt C, Gilis F, Boonen M, Jadot M. Molecular determinants that mediate the sorting of human ATG9A from the endoplasmic reticulum. Biochim. Biophys. Acta Jun 15 2016 12:00AM [PMID:27316455] (IP, Human) IP Human
Staudt C, Gilis F, Boonen M, Jadot M. Molecular determinants that mediate the sorting of human ATG9A from the endoplasmic reticulum. Biochim. Biophys. Acta 2016 Jun 15 [PMID:27316455] (IP, Human) IP Human
Bader CA, Shandala T, Ng YS et al. Atg9 is required for intraluminal vesicles in amphisomes and autolysosomes. Biol Open 2015 Sep 09 [PMID:26353861] (WB, IHC, Drosophila) WB, IHC Drosophila
Ge L, Zhang M, Schekman R. Phosphatidylinositol 3-kinase and COPII generate LC3 lipidation vesicles from the ER-Golgi intermediate compartment. Elife. 2014 Dec 16 [PMID:25432021] (WB, Mouse) WB Mouse
Juarez E, Carranza C, Hernandez-Sanchez F et al. Nucleotide-oligomerizing domain-1 (NOD1) receptor activation induces pro-inflammatory responses and autophagy in human alveolar macrophages. BMC Pulm Med. 2014 Sep 25 [PMID:25253572] (WB, Human) WB Human
Bejarano E, Yuste A, Patel B et al. Connexins modulate autophagosome biogenesis. Nat. Cell Biol. 2014 Apr 06 [PMID:24705551] (WB, ICC/IF, Rat) WB, ICC/IF Rat
Zhang L, Zhang J, Shea K et al. Autophagy in Pancreatic Acinar Cells in Caerulein-treated Mice: Immunolocalization of Related Proteins and Their Potential as Markers of Pancreatitis. Toxicol Pathol 2013 May 2 [PMID:23640381] (WB, Mouse) WB Mouse
Chen H, Luo Z, Dong L et al. CD133/Prominin-1-Mediated Autophagy and Glucose Uptake Beneficial for Hepatoma Cell Survival. PLoS One 2013 [PMID:23437259] (WB, Human) WB Human
Curtis S, Jones CJ, Garrod A et al. Identification of Autophagic Vacuoles and Regulators of Autophagy in Villous Trophoblast from Normal Term Pregnancies and in Fetal Growth Restriction J Matern Fetal Neonatal Med 2012 Oct 8 [PMID:23039021] (IHC, Human) IHC Human
Cant JS, Goodale MA. Scratching beneath the surface: new insights into the functional properties of the lateral occipital area and parahippocampal place area. J Neurosci 2011 Jun 1 [PMID:21632946] (Human) Human
Show All 12 Publications.

Reviews for ATG9A Antibody (NB110-56893) (1) 51

Average Rating: 5
(Based on 1 reviews)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB110-56893:
Filter by Applications
All Applications
Filter by Species
All Species
Images Ratings Applications Species Date Details
reviewed by:
WB Human 09/08/2009


ApplicationWestern Blot
Sample TestedHuman brain extract, Sample Amount: 40 ug
CommentsI was very happy to use the product and another one for LC3 from your company and would gladly recommend it for any one interested.<br />


Blocking DetailsBlocking Buffer: 4% Milk in Tris buffered saline, Blocking Time: 1 hour, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioDilution Ratio: 1/1000, Incubation Dilution Buffer:1% BSA in PBS with 0.1 % tween 20, Incubation Time: 2 hr, Incubation Temp: RT

Secondary Antibody

Secondary DescriptionSecondary Ab: Anti rabbit HRP, Secondary Ab Dilution Ratio: 1/5000


Detection NotesDetection Method: Chemiluminescence, Exposure Time: 1-5 min, Specific Bands: 90 kDa, Non-Specific Bands: minor


CommentsI was very happy to use the product and another one for LC3 from your company and would gladly recommend it for any one interested.<br />

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ATG9A Antibody (NB110-56893) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB110-56893AF405
Alexa Fluor (R) 488 Labeled NB110-56893AF488
Alexa Fluor (R) 647 Labeled NB110-56893AF647
Alexa Fluor (R) 700 Labeled NB110-56893AF700
Biotin Labeled NB110-56893B
DyLight 405 Labeled NB110-56893V
DyLight 488 Labeled NB110-56893G
DyLight 550 Labeled NB110-56893R
DyLight 650 Labeled NB110-56893C
FITC Labeled NB110-56893F
HRP Labeled NB110-56893H

Additional ATG9A Antibody Products

Related Products by Gene

Bioinformatics Tool for ATG9A Antibody (NB110-56893)

Discover related pathways, diseases and genes to ATG9A Antibody (NB110-56893). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ATG9A Antibody (NB110-56893)

Discover more about diseases related to ATG9A Antibody (NB110-56893).

Pathways for ATG9A Antibody (NB110-56893)

View related products by pathway.

PTMs for ATG9A Antibody (NB110-56893)

Learn more about PTMs related to ATG9A Antibody (NB110-56893).

Research Areas for ATG9A Antibody (NB110-56893)

Find related products by research area.

Blogs on ATG9A.

Atg9b - a marker for autophagosome induction and assembly
Atg9 is the only essential transmembrane protein involved in cellular autophagy. Autophagy regulates cellular homeostasis by allowing the turnover and recycling of misfolded proteins and damaged organelles. Formation of the double-membrane isolatio...  Read full blog post.

ATG9A - early marker autophagosome assembly
ATG9A is the only essential integral membrane protein involved in autophagy. ATG9A contains six transmembrane domains and initiates the assembly of autophagosomes. The autophagosome is a double-membrane structure that engulfs and eventually degrade...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol ATG9A

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NB110-56893 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought