ATG9A Antibody

Images

 
Western Blot: ATG9A Antibody [NB110-56893] - Detection of ATG9A protein in HEK293 lysates using NB110-56893. Lane 1: siRNA ATG9A knockdown Lane 2: wildtype ATG9A.
Immunohistochemistry: ATG9A Antibody [NB110-56893] - Staining in mouse intestine.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ch, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml
     

Genetic Strategies

   

ATG9A Antibody Summary

Immunogen
A synthetic peptide made to a region within the C-terminus of human ATG9A (between residues 750-839). [Swiss-Prot# Q7Z3C6].
Localization
Membrane; multi-pass membrane protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATG9A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:100-1:250
  • Immunohistochemistry-Paraffin 1:100-1:250
Application Notes
This ATG9A antibody is useful for Immunohistochemistry paraffin embedded sections and Western blot. In Western blot this antibody recognizes a band at ~95 kDa, representing ATG9A protein. Boiling samples prior to running the gel may affect the protein. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 24705551) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
95 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
ATG9A Peptide (NB110-56893PEP)
Reviewed Applications
Read 1 Review rated 5
using
NB110-56893 in the following applications:

Publications
Read Publications using
NB110-56893 in the following applications:

  • 1 publication
  • IHC
    3 publications
  • IP
    1 publication
  • WB
    9 publications

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ATG9A Antibody

  • APG9 autophagy 9-like 1 (S. cerevisiae)
  • APG9 autophagy 9-like 1
  • APG9L1APG9-like 1
  • ATG9 autophagy related 9 homolog A (S. cerevisiae)
  • autophagy 9-like 1 protein
  • autophagy-related protein 9A
  • FLJ22169
  • mATG9
  • MGD3208

Background

ATG9A (autophagy-related protein 9A) is the only transmembrane member identified among the classical ATG proteins family which are essential to autophagy. Yeast ATG9 protein localizes to the pre-autophagosomal structure (PAS) as well as cytoplasmic punctate structures, whereas, the mammalian ATG9A localizes to endocytic compartments, the trans-Golgi network and autophagic membranes. Moreover, ATG9 is recruited to the PAS in an ATG11-dependent manner under nutrient-rich conditions, whereas during starvation, recruitment of ATG9 depends on Atg17p. ATG9A does not steadily reside at one place in cells, but rather dynamically shuttles between a juxta-nuclear trans-Golgi network compartment and late endosomes under autophagic or starvation conditions. Moreover, ATG9A accumulation is upregulated upon starvation in autophagosomes and the starvation-dependent trafficking requires ULK1, ATG13 and SUPT20H. ATG9A interacts with SUPT20H and has been proposed to mediate membrane transport to generate autophagosomes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, Bv, Ch, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P

Publications for ATG9A Antibody (NB110-56893)(14)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Drosophila.

We have publications tested in 4 applications: ICC/IF, IHC, IP, WB.


Filter By Application
ICC/IF
(1)
IHC
(3)
IP
(1)
WB
(9)
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Filter By Species
Human
(6)
Mouse
(4)
Rat
(1)
Drosophila
(1)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using NB110-56893 Applications Species
Ugun-Klusek A, Tatham MH, Elkharaz J et al. Continued 26S proteasome dysfunction in mouse brain cortical neurons impairs autophagy and the Keap1-Nrf2 oxidative defence pathway Cell Death Dis Jan 5 2017 [PMID: 28055010] (IHC, Mouse) IHC Mouse
Staudt C, Gilis F, Boonen M, Jadot M. Molecular determinants that mediate the sorting of human ATG9A from the endoplasmic reticulum. Biochim. Biophys. Acta Jun 15 2016 [PMID: 27316455] (IP, Human) IP Human
Cheong JW, Kim Y, Eom JI et al. Enhanced autophagy in cytarabine arabinoside-resistant U937 leukemia cells and its potential as a target for overcoming resistance. Mol Med Rep 2016 Apr [PMID: 26935591] (WB) WB
Bader CA, Shandala T, Ng YS et al. Atg9 is required for intraluminal vesicles in amphisomes and autolysosomes. Biol Open 2015 Sep 09 [PMID: 26353861] (WB, IHC, Drosophila) WB, IHC Drosophila
Mitter SK, Song C, Qi X et al. Dysregulated autophagy in the RPE is associated with increased susceptibility to oxidative stress and AMD. Autophagy 2014 [PMID: 25484094] (WB) WB
Ge L, Zhang M, Schekman R. Phosphatidylinositol 3-kinase and COPII generate LC3 lipidation vesicles from the ER-Golgi intermediate compartment. Elife. 2014 Dec 16 [PMID: 25432021] (WB, Mouse) WB Mouse
Juarez E, Carranza C, Hernandez-Sanchez F et al. Nucleotide-oligomerizing domain-1 (NOD1) receptor activation induces pro-inflammatory responses and autophagy in human alveolar macrophages. BMC Pulm Med. 2014 Sep 25 [PMID: 25253572] (WB, Human) WB Human
Bejarano E, Yuste A, Patel B et al. Connexins modulate autophagosome biogenesis. Nat. Cell Biol. 2014 Apr 06 [PMID: 24705551] (WB, ICC/IF, Rat) WB, ICC/IF Rat
Zhang L, Zhang J, Shea K et al. Autophagy in Pancreatic Acinar Cells in Caerulein-treated Mice: Immunolocalization of Related Proteins and Their Potential as Markers of Pancreatitis. Toxicol Pathol 2013 May 2 [PMID: 23640381] (WB, Mouse) WB Mouse
Chen H, Luo Z, Dong L et al. CD133/Prominin-1-Mediated Autophagy and Glucose Uptake Beneficial for Hepatoma Cell Survival. PLoS One 2013 [PMID: 23437259] (WB, Human) WB Human
Show All 14 Publications.

Review for ATG9A Antibody (NB110-56893) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB110-56893:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 09/08/2009
View

Summary

ApplicationWestern Blot
Sample TestedHuman brain extract, Sample Amount: 40 ug
SpeciesHuman
LotSSB
CommentsI was very happy to use the product and another one for LC3 from your company and would gladly recommend it for any one interested.

Blocking

Blocking DetailsBlocking Buffer: 4% Milk in Tris buffered saline, Blocking Time: 1 hour, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioDilution Ratio: 1/1000, Incubation Dilution Buffer:1% BSA in PBS with 0.1 % tween 20, Incubation Time: 2 hr, Incubation Temp: RT

Secondary Antibody

Secondary DescriptionSecondary Ab: Anti rabbit HRP, Secondary Ab Dilution Ratio: 1/5000

Details

Detection NotesDetection Method: Chemiluminescence, Exposure Time: 1-5 min, Specific Bands: 90 kDa, Non-Specific Bands: minor

Comments

CommentsI was very happy to use the product and another one for LC3 from your company and would gladly recommend it for any one interested.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ATG9A Antibody (NB110-56893) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-56893AF405
Alexa Fluor 488 Labeled NB110-56893AF488
Alexa Fluor 647 Labeled NB110-56893AF647
Alexa Fluor 700 Labeled NB110-56893AF700
Biotin Labeled NB110-56893B
DyLight 405 Labeled NB110-56893V
DyLight 488 Labeled NB110-56893G
DyLight 550 Labeled NB110-56893R
DyLight 650 Labeled NB110-56893C
FITC Labeled NB110-56893F
HRP Labeled NB110-56893H

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Bioinformatics Tool for ATG9A Antibody (NB110-56893)

Discover related pathways, diseases and genes to ATG9A Antibody (NB110-56893). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on ATG9A.

Atg9b - a marker for autophagosome induction and assembly
Atg9 is the only essential transmembrane protein involved in cellular autophagy. Autophagy regulates cellular homeostasis by allowing the turnover and recycling of misfolded proteins and damaged organelles. Formation of the double-membrane isolatio...  Read full blog post.

ATG9A - early marker autophagosome assembly
ATG9A is the only essential integral membrane protein involved in autophagy. ATG9A contains six transmembrane domains and initiates the assembly of autophagosomes. The autophagosome is a double-membrane structure that engulfs and eventually degrade...  Read full blog post.

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Recent Reviews

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Anonymous
09/08/2009
Application: WB
Species: Human

Bioinformatics

Gene Symbol ATG9A