ATG16L1 Antibody [DyLight 650]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 650

ATG16L1 Antibody [DyLight 650] Summary

Immunogen
A synthetic peptide within residues 1-100 of mouse ATG16L1. [Swiss-Prot# Q8C0J2]
Localization
Cytoplasm. Preautophagosomal structure, preautophagosomal structure membrane, peripheral membrane protein. Note: Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATG16L1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This ATG16L1 antibody is useful for Western blot, where a band can be seen ~66 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for ATG16L1 Antibody [DyLight 650]

  • APG16 autophagy 16-like (S. cerevisiae)
  • APG16L beta
  • APG16LFLJ10828
  • APG16-like 1
  • ATG16 autophagy related 16-like (S. cerevisiae)
  • ATG16 autophagy related 16-like 1 (S. cerevisiae)
  • ATG16A
  • ATG16L
  • autophagy-related protein 16-1
  • FLJ00045
  • FLJ10035
  • FLJ22677
  • IBD10
  • WD repeat domain 30
  • WDR30

Background

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). The APG12-APG5-APG16L complex is essential for the elongation of autophagic isolation membranes. This complex initially associates in uniform distribution with small vesicle membranes. During membrane elongation, the complex partitions, with a great concentration building on the outer side of the isolation membrane. Upon completion of the formation of the autophagosome, the APG12-APG5-APG16L dissociates from the membrane.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB110-82384C
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Applications: WB

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ATG16L1 Antibody (NB110-82384C) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB110-82384AF350
Alexa Fluor 405 NB110-82384AF405
Alexa Fluor 488 NB110-82384AF488
Alexa Fluor 532 NB110-82384AF532
Alexa Fluor 594 NB110-82384AF594
Alexa Fluor 647 NB110-82384AF647
Alexa Fluor 700 NB110-82384AF700
Alexa Fluor 750 NB110-82384AF750
Biotin NB110-82384B
DyLight 350 NB110-82384UV
DyLight 405 NB110-82384V
DyLight 488 NB110-82384G
DyLight 550 NB110-82384R
DyLight 594 NB110-82384DL594
DyLight 650 NB110-82384C
DyLight 680 NB110-82384FR
DyLight 755 NB110-82384IR
HRP NB110-82384H

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Bioinformatics Tool for ATG16L1 Antibody (NB110-82384C)

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Blogs on ATG16L1.

Liver ASK1 activates autophagy to protect against hepatic fat accumulation, non-alcoholic steatohepatitis and fibrosis
By Jamshed Arslan, Pharm. D., PhD. The most common chronic liver disorder worldwide is non-alcoholic fatty liver disease (NAFLD). This obesity-linked disorder can manifest as hepatic fat accumulation (steatosis) wit...  Read full blog post.

ATG16L1 - a key player in the development of the autophagosome
Like apoptosis, autophagy is a highly regulated physiologic process that involves cellular degradation and recycling of organelles and macromolecules. Autophagy is a survival mechanism induced by states of stress, starvation, and infection. A do...  Read full blog post.

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Bioinformatics

Gene Symbol ATG16L1