Aromatase Antibody [FITC]

• Reactivity: Hu, Mu, Rt, Pm, Rb, Bv, Eq
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: FITC

Aromatase Antibody [FITC] Summary

• Immunogen: A synthetic peptide made to a C-terminal portion of the human Aromatase protein (between residues 400-502). [UniProt# P11511]
• Localization: Membrane-bound
• Predicted Species: Bovine (90%), Equine (91%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CYP19A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Use in Rabbit reported in scientific literature (PMID:35245530). Rabbit reactivity reported in scientific literature (PMID: 25850953). Immunogen displays the following percentage of sequence identity for non-tested species: horse (91%), cow (90%), pig (83%), sheep (81%), goat (81%), and dog (81%) proteins. Rat reactivity reported in scientific literature (PMID: 29898754). Use in Mouse reported in scientific literature (PMID:32634520).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Aromatase Antibody [FITC]

• ARO1
• ARO1EC 1.14.14.1
• Aromatase
• AROsubfamily XIX (aromatization of androgens)
• CYAR
• CYARMGC104309
• CYP19
• CYP19A1
• CYPXIX
• cytochrome P450, family 19, subfamily A, polypeptide 1
• Cytochrome P-450AROM
• EC 1.14.14.14
• Estrogen synthase
• microsomal monooxygenase

Background

Aromatase (ARO1) or cytochrome P450 19A1 (CYP19A1) belongs to cytochrome P450 family of enzymes, which are monooxygenases known for their essential role in drug catabolism and cholesterol/steroid synthesis. Employing heme-group act as a co-factor, ARO1 catalyzes aromatic C18 estrogens formation from C19 androgens and is responsible for conversion of testosterone to estradiol and androstenedione to estrone. ARO1 is localized to cellular membranes as a peripheral membrane protein and in addition to female reproductive tract and adipose tissue, aromatase can be found in testis, liver, brain as well as certain tumors including breast cancer. During ontogenesis, it plays an important role in the development of CNS and gonads as well as sex differentiation. Defective ARO1 may leads to aromatase excess syndrome (AEXS) and aromatase deficiency (AROD). Moreover, obesity has been linked to abnormally high ARO1 expression in mammary tissues, increased local estrogen production, and susceptibility to breast hyperplasia and cancer. ARO1 is the key enzyme in intratumoral estrogen production in postmenopausal mammary cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CYP19A1