Progesterone R/NR3C3 Antibody [Unconjugated]

HAF016). Specific bands were detected for PR-A and PR-B at approximately 90 and 118 kDa, respectively (as indicated). This experiment was conducted using Immunoblot Buffer Group 1." > HAF016). Specific bands were detected for PR-A and PR-B at approximately 90 and 118 kDa, respectively (as indicated). This experiment was conducted using Immunoblot Buffer Group 1." title="Western blot shows lysates of T47D human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Progesterone R/NR3C3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5415) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for PR-A and PR-B at approximately 90 and 118 kDa, respectively (as indicated). This experiment was conducted using Immunoblot Buffer Group 1." />

Western blot shows lysates of T47D human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Progesterone R/NR3C3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5415) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=

NL010) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." > NL010) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." title="Progesterone R/NR3C3 was detected in immersion fixed T47D human breast cancer cell line using Sheep Anti-Human Progesterone R/NR3C3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5415) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." />

Progesterone R/NR3C3 was detected in immersion fixed T47D human breast cancer cell line using Sheep Anti-Human Progesterone R/NR3C3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5415) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # <a class=

CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." > CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." title="Progesterone R/NR3C3 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Sheep Anti-Human Progesterone R/NR3C3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5415) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." />

Progesterone R/NR3C3 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Sheep Anti-Human Progesterone R/NR3C3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5415) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." > HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." title="Simple Western lane view shows lysates of T47D human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Progesterone R/NR3C3 at approximately 122 kDa (as indicated) using 25 µg/mL of Sheep Anti-Human Progesterone R/NR3C3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5415) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." />

Simple Western lane view shows lysates of T47D human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Progesterone R/NR3C3 at approximately 122 kDa (as indicated) using 25 µg/mL of Sheep Anti-Human Progesterone R/NR3C3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5415) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (<a class=

• Reactivity: Hu
• Applications: WB, Simple Western, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Progesterone R/NR3C3 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human PR-B
  Met1-Leu189
  Accession # P06401
• Specificity: Detects human PR-A and PR-B in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: PGR
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 1-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Progesterone R/NR3C3 Antibody [Unconjugated]

• NR3C3
• NR3C3PRNuclear receptor subfamily 3 group C member 3
• PGR
• Progesterone R
• progesterone receptor
• ProgesteroneR

Background

Progesterone Receptor B (PR-B) is a 118 kDa member of the NR3 subfamily within the nuclear hormone receptor family of proteins. It is expressed in female reproductive tissues as well as neurons throughout the CNS. PR-B is particularly important in the mammary gland where it mediates proliferative responses to progesterone. Human PR-B is 933 amino acids (aa) in length. It contains an N-terminal regulatory region (aa 1‑566), a DNA binding domain (aa 567‑639), and a
steroid-binding region (aa 681‑933). Ligand binding induces a key phosphorylation event at Ser294 by ERK1/2. An alternate start site at Met165 generates 90 kDa
PR‑A, an isoform particularly important in the ovary and uterus that insures fertility. Other isoforms show a deletion of either aa 637‑738 or 598‑636, or a 17 aa substitution for aa 787‑933.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Progesterone R/NR3C3 Antibody (AF5415)(2)

Publications using AF5415

• Hannah M. Peterson, Lip Ket Chin, Yoshi Iwamoto, Juhyun Oh, Jonathan C. T. Carlson, Hakho Lee et al. Integrated Analytical System for Clinical Single‐Cell Analysis Advanced Science 7/1/2022 [PMID: 35508767]
• Schillace R, Skinner A, Pommier R, O'Neill S, Muller P, Naik A, Hansen J, Pommier S Estrogen receptor, progesterone receptor, interleukin-6 and interleukin-8 are variable in breast cancer and benign stem/progenitor cell populations. BMC Cancer, 2014-09-30;14(0):733. 2014-09-30 [PMID: 25269750] (Functional Assay, Human)

FAQs for Progesterone R/NR3C3 Antibody (AF5415). (Showing 1 - 1 of 1 FAQs).

1. I am interested in getting antibodies for PR (progesterone receptor) that would work in flow cytometry. Could you recommend some choices?
   • We do not currently have any antibodies directed towards the progesterone receptor that have been validated in flow cytometry, but any of our antibodies that work in ICC should work just fine in flow as they are essentially the same application. Please see this link for our Progesterone Receptor antibodies validated in ICC.

Bioinformatics

• Gene Symbol: PGR
• Uniprot:
  • P06401()