Progesterone R/NR3C3 Antibody [Unconjugated]

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Western blot shows lysates of T47D human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Progesterone R/NR3C3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5415) followed ...read more
Progesterone R/NR3C3 was detected in immersion fixed T47D human breast cancer cell line using Sheep Anti-Human Progesterone R/NR3C3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5415) at 1.7 µg/mL ...read more
Progesterone R/NR3C3 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Sheep Anti-Human Progesterone R/NR3C3 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

Progesterone R/NR3C3 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human PR-B
Met1-Leu189
Accession # P06401
Specificity
Detects human PR-A and PR-B in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
PGR
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunocytochemistry 1-15 ug/mL
Publications
Read Publication using
AF5415 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Progesterone R/NR3C3 Antibody [Unconjugated]

  • NR3C3
  • NR3C3PRNuclear receptor subfamily 3 group C member 3
  • PGR
  • Progesterone R
  • progesterone receptor
  • ProgesteroneR

Background

Progesterone Receptor B (PR-B) is a 118 kDa member of the NR3 subfamily within the nuclear hormone receptor family of proteins. It is expressed in female reproductive tissues as well as neurons throughout the CNS. PR-B is particularly important in the mammary gland where it mediates proliferative responses to progesterone. Human PR-B is 933 amino acids (aa) in length. It contains an N-terminal regulatory region (aa 1‑566), a DNA binding domain (aa 567‑639), and a
steroid-binding region (aa 681‑933). Ligand binding induces a key phosphorylation event at Ser294 by ERK1/2. An alternate start site at Met165 generates 90 kDa
PR‑A, an isoform particularly important in the ovary and uterus that insures fertility. Other isoforms show a deletion of either aa 637‑738 or 598‑636, or a 17 aa substitution for aa 787‑933.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Progesterone R/NR3C3 Antibody (AF5415)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Functional Assay.


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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Progesterone R/NR3C3 Antibody (AF5415). (Showing 1 - 1 of 1 FAQ).

  1. I am interested in getting antibodies for PR (progesterone receptor) that would work in flow cytometry. Could you recommend some choices?
    • We do not currently have any antibodies directed towards the progesterone receptor that have been validated in flow cytometry, but any of our antibodies that work in ICC should work just fine in flow as they are essentially the same application. Please see this link for our Progesterone Receptor antibodies validated in ICC.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PGR
Uniprot