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CYP11A1 Antibody

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 (4 Reviews)
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Western Blot: CYP11A1 Antibody [NBP1-85368] - protein extract from: 1) human liver (negative), 2) human testis (positve), 3) MW marker (drawn with special ECL reagent marker), 4) human testis, 5) H295R adrenal cell line. Image submitted by a verified customer review.
Western Blot: CYP11A1 Antibody [NBP1-85368] - protein extract from: 1) human liver (negative), 2) human testis (positve), 3) MW marker (drawn with special ECL reagent marker), 4) human testis, 5) H295R adrenal cell ...read more
Immunocytochemistry/ Immunofluorescence: CYP11A1 Antibody [NBP1-85368] - CYP11A1 expression (Leydig cells) present in early pubertal Rhesus monkey. Secondary antibody: Alexa Fluor488 anti rabbit. Image submitted by a ...read more
Immunohistochemistry-Paraffin: CYP11A1 Antibody [NBP1-85368] - Staining of human cerebral cortex shows low expression as expected.
Immunohistochemistry-Paraffin: CYP11A1 Antibody [NBP1-85368] - Staining of human adrenal gland shows high expression.
Orthogonal Strategies: Immunohistochemistry-Paraffin: CYP11A1 Antibody [NBP1-85368] - Staining in human adrenal gland and cerebral cortex tissues using anti-CYP11A1 antibody. Corresponding CYP11A1 RNA-seq data ...read more

Product Details

Summary
Reactivity Hu, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

  

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CYP11A1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: RSVLVKGCQTFLSAPREGLGRLRVPTGEGAGISTRSPRPFNEIPSPGDNGWLNLYHFWRETGTHKVHLHHVQNFQKYGPIYREKLGNVESVYVIDPEDVALLFKSEGPNPERFLIPPWVAYHQYYQRPIGVLLKKSAA
Specificity
Specificity of human CYP11A1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CYP11A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 0.04-0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. CYP11A1 antibody validated for ICC/IF, WB from verified customer reviews.
Control Peptide
CYP11A1 Protein (NBP1-85368PEP)
Reviewed Applications
Read 4 Reviews rated 5
using
NBP1-85368 in the following applications:
Publications
Read Publication using NBP1-85368.

Reactivity Notes

Rhesus monkey reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CYP11A1 Antibody

  • Cholesterol desmolase
  • cholesterol monooxygenase (side-chain-cleaving)
  • cholesterol side-chain cleavage enzyme, mitochondrial
  • CYPXIA1
  • Cytochrome P450 11A1
  • Cytochrome P450(scc)
  • cytochrome P450, family 11, subfamily A, polypeptide 1
  • cytochrome P450C11A1
  • EC 1.14.15
  • EC 1.14.15.6
  • steroid 20-22-lyase
  • subfamily XIA (cholesterol side chain cleavage)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: StAR Antibody [NBP1-33485] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with StAR antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody
Immunocytochemistry/Immunofluorescence: StAR Antibody [NBP1-33485] - HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: StAR protein stained by StAR antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: StAR Antibody [NBP1-33485] - Human hepatoma. StAR antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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Western Blot: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytochrome P450 17A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cytochrome P450 17A1.
Immunohistochemistry: Cytochrome P450 17A1 Antibody (OTI3F11) [NBP2-01151] - Staining in macaque Leydig cells. Image from a verified customer review.
Flow Cytometry: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Analysis of Jurkat cells, using anti-Cytochrome P450 17A1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
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(1 Publication)
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SF-1/NR5A1/Steroidogen ...
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Western Blot: SF-1/NR5A1/Steroidogenic Factor 1 Antibody [NBP1-52823] - Titration: 0.2-1 ug/ml, Positive Control: THP-1 cell lysate.
Immunohistochemistry-Paraffin: SF-1/NR5A1/Steroidogenic Factor 1 Antibody [NBP1-52823] - Human adrenal tissue at an antibody concentration of 4-8ug/ml.
(3 Publications)
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Western Blot: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) and hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase. Image collected and cropped by CiteAb from the following publication (https://www.hindawi.com/journals/bmri/2017/3795950/), licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: Aromatase Antibody [NB100-1596] - Aromatase antibody was tested in SH-SY-5Y cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry-Paraffin: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control rabbits. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of control ovaries. Nonlabeling was observed when the primary antibody was omitted. Bars: 200 um (1st image) and 20 um (2nd image). Granulose cells, gc; theca cells, tc. Image collected and cropped by CiteAb from the following publication (https://www.hindawi.com/journals/bmri/2017/3795950/), licensed under a CC-BY licence.
(6 Publications)
Species: Hu, Rt, Pm, Rb
Applications: WB, ICC/IF, IHC, IHC-P
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Western Blot: POMC Antibody [NB100-1533] - Staining of NIH3T3 cell lysate with antibody at 0.3 ug/mL (A) and Rat Brain cell lysate with antibody at 2 ug/mL (B). 35 ug protein in RIPA buffer. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: POMC Antibody [NB100-1533] - Staining of paraffin embedded Human Pituitary Gland. Antibody at 2 ug/mL. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
Flow Cytometry: POMC Antibody [NB100-1533] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
(10 Publications)
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NBP1-68883
Western Blot: CYP11B1 Antibody [NBP1-68883] - Titration: 0.2-1 ug/ml, Positive Control: Human Liver.
Immunohistochemistry: CYP11B1 Antibody [NBP1-68883] - Monkey vagina Primary Antibody Dilution: 1 : 25 Secondary Antibody: Anti-rabbit-HRP Secondary Antibody Dilution: 1 : 1000 Color/Signal Descriptions: Brown: CYP11B1 Blue: Nucleus Gene name: CYP11B1 Submitted by: Jonathan Bertin, Endoceutics Inc.
Immunohistochemistry: CYP11B1 Antibody [NBP1-68883] - Monkey adrenal gland Primary Antibody Dilution: 1 : 25 Secondary Antibody: Anti-rabbit-HRP Secondary Antibody Dilution: 1 : 1000 Color/Signal Descriptions: Brown: CYP11B1 Blue: Nucleus Gene name: CYP11B1 Submitted by: Jonathan Bertin, Endoceutics Inc.
Species: Hu, Pm
Applications: WB, IHC
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Immunohistochemistry: HSD3B1 Antibody (FDO66Q) [NB110-78644] - Staining of placental villi.
(7 Publications)
Species: Hu, Rt, Po, Pm, Bv, Gt, Pm
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Western Blot: CYP11B2 Antibody [NBP2-92862] - Analysis of extracts of various cell lines, using CYP11B2.Exposure time: 20s.
Immunocytochemistry/Immunofluorescence: CYP11B2 Antibody [NBP2-92862] - Analysis of HeLa cells using CYP11B2. Blue: DAPI for nuclear staining.
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Species: Hu, Mu, Rt
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Western Blot: BRD2 Antibody [NBP1-30475] - Detection of Human and Mouse BRD2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD2 antibody NBP1-30475 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunocytochemistry/Immunofluorescence: BRD2 Antibody [NBP1-30475] - Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti- BRD2 used at a dilution of 1:100 (2ug/ml) (left) and rabbit anti-BRD2 recombinant monoclonal used at a dilution of 1:20 (right). Detection: Red-fluorescent goat anti-rabbit IgG cross-adsorbed Antibody DyLight 594 conjugated.
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(1 Publication)
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
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Western Blot: PLOD1 Antibody [NBP2-38770] - Analysis in human cell line U-87 MG.
Western Blot: PLOD1 Antibody [NBP2-38770] - SC65 directly interacts with lysyl-hydroxylase 1 (LH1). Western blot of primary calvarial osteoblast and skin fibroblast lysates from WT and Sc65KO 3 day-old mice (N = 2) showing significantly decreased levels of LH1 protein in Sc65KO samples. Densitometric quantification of LH1 protein normalized to beta-actin from the western blot shown above (*p<0.05; error bars represent SD). All experiments were performed at least 3 times. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pgen.1006002), licensed under a CC-BY licence.
Western Blot: PLOD1 Antibody [NBP2-38770] - Analysis in human cell line U-251 MG and human cell line RT-4.
(2 Publications)
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Western Blot: CYP21A2 Antibody [NBP2-38698] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Adrenal Gland tissue
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Immunohistochemistry-Paraffin: CYP21A2 Antibody [NBP2-38698] - Staining of human adrenal gland shows high expression.
Species: Hu
Applications: WB, Simple Western, IHC, IHC-P
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Immunocytochemistry/Immunofluorescence: Ferredoxin Reductase Antibody [NBP2-38530] - Staining of human cell line U-2 OS shows positivity in mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Ferredoxin Reductase Antibody [NBP2-38530] - Staining of human ovary.
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Western Blot: Androgen R/NR3C4 [p Ser213, p Ser210] Antibody (156C135.2) [NB100-56603] - Analysis using Azide Free version of NB100-56603. LNCaP cells (passage number 38) were serum-starved for 2 days. After serum starvation, cells were (A) left untreated, (B) treated with 100 ng/ml IGF-1 for 4h, or (C) incubated with 20 um LY294002 for 30 mi
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Immunohistochemistry-Paraffin: PBR Antibody [NB100-41398] - Staining of paraffin embedded Human Kidney. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: PBR Antibody [NB100-41398] - Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Immunohistochemistry-Paraffin: AKR1B1 Antibody [NBP1-89146] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: AKR1B1 Antibody [NBP1-89146] - Staining of human cerebral cortex shows low expression as expected.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
CYP11A1 Antibody
NBP1-85368
CYP11A1 Antibody
Species: Hu, RM
Applications: WB, ICC/IF, IHC, IHC-P

Publications for CYP11A1 Antibody (NBP1-85368)(1)


Reviews for CYP11A1 Antibody (NBP1-85368) (4) 54

Average Rating: 5
(Based on 4 reviews)
We have 4 reviews tested in 2 species: Human, Rhesus macaque.

Reviews using NBP1-85368:
Filter by Applications
IHC-P
(2)
WB
(1)
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Human
(2)
Rhesus macaque
(2)
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Images Ratings Applications Species Date Details
CYP11A1 NBP1-85368
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reviewed by:
Paula Aliberti
 Rhesus macaque 03/09/2018
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Summary

Sample TestedEarly pubertal testis
SpeciesRhesus macaque
LotB106749

Comments

CommentsPrimary antibody dilution 1:250.
Secondary antibody used: Alexa Fluor 488 anti rabbit
Immunohistochemistry-Paraffin CYP11A1 NBP1-85368
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reviewed by:
Paula Aliberti
 IHC-P Rhesus macaque 11/24/2017
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedpubertal testis
SpeciesRhesus macaque
LotB106749
Immunohistochemistry-Paraffin CYP11A1 NBP1-85368
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reviewed by:
Paula Aliberti
 IHC-P Human 10/09/2017
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman testis
SpeciesHuman
LotB106749

Comments

CommentsAntibody diluted 1/250
Western Blot CYP11A1 NBP1-85368
Enlarge
reviewed by:
Paula Aliberti
 WB Human 10/09/2017
View

Summary

ApplicationWestern Blot
Sample TestedHuman liver,Human testis,Whole cell lysate and mitochondrial extract of H295R cells (human adrenal cortical cells)
SpeciesHuman
LotB106749

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FAQs for CYP11A1 Antibody (NBP1-85368) (0)

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Recent Reviews

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CYP11A1 NBP1-85368
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 Paula Aliberti
03/09/2018
Application:
Species: Rhesus macaque
read this review
Immunohistochemistry-Paraffin CYP11A1 NBP1-85368
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 Paula Aliberti
11/24/2017
Application: IHC-P
Species: Rhesus macaque
read this review
Immunohistochemistry-Paraffin CYP11A1 NBP1-85368
Enlarge
 Paula Aliberti
10/09/2017
Application: IHC-P
Species: Human
read this review

Bioinformatics

Gene Symbol CYP11A1