CYP11A1 Antibody

Immunohistochemistry-Paraffin: CYP11A1 Antibody [NBP1-85368] - Staining in human adrenal gland and cerebral cortex tissues using anti-CYP11A1 antibody. Corresponding CYP11A1 RNA-seq data are presented for the same tissues.

Western Blot: CYP11A1 Antibody [NBP1-85368] - protein extract from: 1) human liver (negative), 2) human testis (positve), 3) MW marker (drawn with special ECL reagent marker), 4) human testis, 5) H295R adrenal cell line. Image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: CYP11A1 Antibody [NBP1-85368] - CYP11A1 expression (Leydig cells) present in early pubertal Rhesus monkey. Secondary antibody: Alexa Fluor488 anti rabbit. Image submitted by a verified customer review.

Immunohistochemistry-Paraffin: CYP11A1 Antibody [NBP1-85368] - Staining of human cerebral cortex shows low expression as expected.

Immunohistochemistry-Paraffin: CYP11A1 Antibody [NBP1-85368] - Staining of human adrenal gland shows high expression.

• Reactivity: Hu, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

CYP11A1 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: RSVLVKGCQTFLSAPREGLGRLRVPTGEGAGISTRSPRPFNEIPSPGDNGWLNLYHFWRETGTHKVHLHHVQNFQKYGPIYREKLGNVESVYVIDPEDVALLFKSEGPNPERFLIPPWVAYHQYYQRPIGVLLKKSAA
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CYP11A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. CYP11A1 antibody validated for ICC/IF, WB from verified customer reviews.
• Control Peptide:

  CYP11A1 Protein (NBP1-85368PEP)

Reactivity Notes

Rhesus monkey reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for CYP11A1 Antibody

• Cholesterol desmolase
• cholesterol monooxygenase (side-chain-cleaving)
• cholesterol side-chain cleavage enzyme, mitochondrial
• CYPXIA1
• Cytochrome P450 11A1
• Cytochrome P450(scc)
• cytochrome P450, family 11, subfamily A, polypeptide 1
• cytochrome P450C11A1
• EC 1.14.15
• EC 1.14.15.6
• steroid 20-22-lyase
• subfamily XIA (cholesterol side chain cleavage)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CYP11A1 Antibody (NBP1-85368)(3)

Publications using NBP1-85368

• Ali HE, Scoggin K, Murase H et al. Transcriptomic and histochemical analysis reveal the complex regulatory networks in equine Chorioallantois during spontaneous term labor Biology of reproduction 2022-08-01 [PMID: 35913756] (IHC-P, Human)
• El-Sheikh Ali H, Loux Sc, Kennedy L Et Al. Transcriptomic analysis of equine chorioallantois reveals immune networks and molecular mechanisms involved in nocardioform placentitis Veterinary research 2021-07-08 [PMID: 34238364] (IF/IHC)
• Arboleda VA, Lee H, Parnaik R et al. Mutations in the PCNA-binding domain of CDKN1C cause IMAGe syndrome. Nat Genet 2012-05-01 [PMID: 22634751]

Reviews for CYP11A1 Antibody (NBP1-85368) (4)

Average Rating: 5

(Based on 4 reviews)

We have 4 reviews tested in 2 species: Human, Rhesus Macaque.

reviewed by: Verified Customer Immunofluorescence-Paraffin Rhesus Macaque 03/09/2018

Summary

• Application: Other
• Sample Tested: Early pubertal testis
• Species: Rhesus Macaque
• Lot: B106749

Comments

• Comments: Primary antibody dilution 1:250.
  Secondary antibody used: Alexa Fluor 488 anti rabbit

reviewed by: Verified Customer IHC-P Rhesus Macaque 11/24/2017

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: pubertal testis
• Species: Rhesus Macaque
• Lot: B106749

reviewed by: Verified Customer IHC-P Human 10/09/2017

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Human testis
• Species: Human
• Lot: B106749

Comments

• Comments: Antibody diluted 1/250

reviewed by: Verified Customer WB Human 10/09/2017

Summary

• Application: Western Blot
• Sample Tested: Human liver,Human testis,Whole cell lysate and mitochondrial extract of H295R cells (human adrenal cortical cells)
• Species: Human
• Lot: B106749

Bioinformatics

• Gene Symbol: CYP11A1