![Immunohistochemistry-Paraffin: CYP11A1 Antibody [NBP1-85368] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize2/CYP11A1-Antibody-Immunohistochemistry-Paraffin-NBP1-85368-img0007.jpg "Immunohistochemistry-Paraffin: CYP11A1 Antibody [NBP1-85368] - Staining of human cerebral cortex shows low expression as expected.")
| Reactivity | Hu, RMSpecies Glossary |
| Applications | IHC, IHC-P |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: RSVLVKGCQTFLSAPREGLGRLRVPTGEGAGISTRSPRPFNEIPSPGDNGWLNLYHFWRETGTHKVHLHHVQNFQKYGPIYREKLGNVESVYVIDPEDVALLFKSEGPNPERFLIPPWVAYHQYYQRPIGVLLKKSAA |
| Specificity | Specificity of human CYP11A1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CYP11A1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
| Publication using NBP1-85368 | Applications | Species |
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| Arboleda VA, Lee H, Parnaik R et al. Mutations in the PCNA-binding domain of CDKN1C cause IMAGe syndrome. Nat Genet 2012 May [PMID: 22634751] |
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Paula Aliberti |
Rhesus macaque | 03/09/2018 |
Summary
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reviewed by:
Paula Aliberti |
IHC-P | Rhesus macaque | 11/24/2017 |
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reviewed by:
Paula Aliberti |
IHC-P | Human | 10/09/2017 |
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reviewed by:
Paula Aliberti |
WB | Human | 10/09/2017 |
Summary
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Secondary Antibodies |
Isotype Controls |
Diseases for CYP11A1 Antibody (NBP1-85368)Discover more about diseases related to CYP11A1 Antibody (NBP1-85368).
| Pathways for CYP11A1 Antibody (NBP1-85368)View related products by pathway.
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PTMs for CYP11A1 Antibody (NBP1-85368)Learn more about PTMs related to CYP11A1 Antibody (NBP1-85368).
| Research Areas for CYP11A1 Antibody (NBP1-85368)Find related products by research area.
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| Paula Aliberti 03/09/2018 |
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| Application: | ||
| Species: | Rhesus macaque |
| Paula Aliberti 11/24/2017 |
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| Application: | IHC-P | |
| Species: | Rhesus macaque |
| Paula Aliberti 10/09/2017 |
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| Application: | IHC-P | |
| Species: | Human |
| Gene Symbol | CYP11A1 |
![Immunohistochemistry-Paraffin: CYP11A1 Antibody [NBP1-85368] - Staining of human adrenal gland shows high expression.](http://images.novusbio.com/fullsize2/CYP11A1-Antibody-Immunohistochemistry-Paraffin-NBP1-85368-img0006.jpg "Immunohistochemistry-Paraffin: CYP11A1 Antibody [NBP1-85368] - Staining of human adrenal gland shows high expression.")
![Immunohistochemistry-Paraffin: CYP11A1 Antibody [NBP1-85368] - Staining in human adrenal gland and cerebral cortex tissues using anti-CYP11A1 antibody. Corresponding CYP11A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/CYP11A1 Antibody-Immunohistochemistry-Paraffin-NBP1-85368-img0008.jpg "Immunohistochemistry-Paraffin: CYP11A1 Antibody [NBP1-85368] - Staining in human adrenal gland and cerebral cortex tissues using anti-CYP11A1 antibody. Corresponding CYP11A1 RNA-seq data are presented for the same tissues.")
![Immunofluorescence: CYP11A1 Antibody [NBP1-85368] - CYP11A1 expression (Leydig cells) present in early pubertal Rhesus monkey. Secondary antibody: Alexa Fluor488 anti rabbit. This image was submitted via customer review.](http://images.novusbio.com/fullsize2/CYP11A1-Antibody-Immunofluorescence-NBP1-85368-img0009.jpg "Immunofluorescence: CYP11A1 Antibody [NBP1-85368] - CYP11A1 expression (Leydig cells) present in early pubertal Rhesus monkey. Secondary antibody: Alexa Fluor488 anti rabbit. This image was submitted via customer review.")
![Western Blot: StAR Antibody [NBP1-33485] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with StAR antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody](http://images.novusbio.com/fullsize2/StAR-Antibody-Western-Blot-NBP1-33485-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: StAR Antibody [NBP1-33485] - HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: StAR protein stained by StAR antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize2/StAR-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33485-img0005.jpg)
![Immunohistochemistry-Paraffin: StAR Antibody [NBP1-33485] - Paraffin-embedded hepatoma. StAR antibody dilution: 1:500.](http://images.novusbio.com/fullsize2/StAR-Antibody-Immunohistochemistry-Paraffin-NBP1-33485-img0004.jpg)
![Western Blot: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytochrome P450 17A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cytochrome P450 17A1.](http://images.novusbio.com/fullsize2/Cytochrome-P450-17A1-Antibody-(3F11)-Western-Blot-NBP2-01151-img0024.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Staining of paraffin-embedded Human tonsil using anti-Cytochrome P450 17A1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize2/Cytochrome-P450-17A1-Antibody-(3F11)-Immunohistochemistry-Paraffin-NBP2-01151-img0023.jpg)
![Flow Cytometry: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Analysis of Jurkat cells, using anti-Cytochrome P450 17A1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize2/Cytochrome-P450-17A1-Antibody-(3F11)-Flow-Cytometry-NBP2-01151-img0014.jpg)
![Western Blot: Aromatase Antibody [NB100-1596] - Detection of aromatase in human fetal temporal lobe lysate using NB 100-1596. ECL exposure, 1-2 minutes.](http://images.novusbio.com/fullsize2/Aromatase Antibody-Western Blot-NB100-1596-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Aromatase Antibody [NB100-1596] - Aromatase antibody was tested in SH-SY-5Y cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize2/Aromatase-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1596-img0004.jpg)
![Western Blot: POMC Antibody [NB100-1533] - Western blot analysis of POMC using NB100-1533 at 0.01ug/ml in NIH 3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/POMC Antibody-Western Blot-NB100-1533-img0001.jpg)
![Immunohistochemistry-Paraffin: POMC Antibody [NB100-1533] - Analysis in human Anterior Pituitary Gland shows vesilulate staining in glandular cells.](http://images.novusbio.com/fullsize2/POMC-Antibody-Immunohistochemistry-Paraffin-NB100-1533-img0003.jpg)
![Western Blot: CYP11B1 Antibody [NBP1-68883] - 1. HEK293TN-GFP-hcyp11B1 (75ug) 2. HEK293TN-GFP-hcyp11B2 (75ug) Primary dilution: 1 : 100 Secondary Antibody: mouse anti-Rabbit HRP Secondary Dilution: 1 : 10,000Film Exposed for: 5 minutesImage Submitted by: Celso Gomez-SanchezMongomery VA Medical Center.](http://images.novusbio.com/fullsize2/CYP11B1 Antibody-Western Blot-NBP1-68883-img0007.jpg)
![Immunohistochemistry: CYP11B1 Antibody [NBP1-68883] - Monkey vagina Primary Antibody Dilution: 1 : 25 Secondary Antibody: Anti-rabbit-HRP Secondary Antibody Dilution: 1 : 1000 Color/Signal Descriptions: Brown: CYP11B1 Blue: Nucleus Gene name: CYP11B1 Submitted by: Jonathan Bertin, Endoceutics Inc.](http://images.novusbio.com/fullsize2/CYP11B1-Antibody-Immunohistochemistry-NBP1-68883-img0006.jpg)
![Western Blot: CYP11B1 Antibody [NBP1-68883] - Sample type: 1. HEK293TN-GFP-hcyp11B1 (75ug) 2. HEK293TN-GFP-hcyp11B2 (75ug) Primary Dilution: 1:100 Secondary Antibody: mouse anti-Rabbit HRP Secondary Dilution: 1:10,000 Film Exposed for: 5 minutes](http://images.novusbio.com/fullsize2/CYP11B1-Antibody-Western-Blot-NBP1-68883-img0002.jpg)
![Immunohistochemistry: HSD3B1 Antibody (FDO66Q) [NB110-78644] - Staining of placental villi.](http://images.novusbio.com/fullsize2/HSD3B1 Antibody (FDO66Q)-Immunohistochemistry-NB110-78644-img0002.jpg)
![Immunohistochemistry-Paraffin: CYP11B2 Antibody [NBP2-13891] - Staining of adrenal gland shows strong cytoplasmic positivity in cortical cells.](http://images.novusbio.com/fullsize2/CYP11B2 Antibody-Immunohistochemistry-Paraffin-NBP2-13891-img0005.jpg)
![Western Blot: BRD2 Antibody [NBP1-30475] - Detection of Human and Mouse BRD2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD2 antibody NBP1-30475 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize2/BRD2-Antibody-Western-Blot-NBP1-30475-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: BRD2 Antibody [NBP1-30475] - Affinity purified rabbit anti-BRD2 used at a dilution of 1:100 (2ug/ml). Detection: Red-fluorescent goat anti-rabbit IgG cross-adsorbed Antibody DyLight 594 conjugated.](http://images.novusbio.com/fullsize2/BRD2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30475-img0013.jpg)
![Immunohistochemistry: BRD2 Antibody [NBP1-30475] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-BRD2 used at a dilution of 1:50 (4ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit](http://images.novusbio.com/fullsize2/BRD2-Antibody-Immunohistochemistry-NBP1-30475-img0017.jpg)
![Western Blot: PLOD1 Antibody [NBP2-38770] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG](http://images.novusbio.com/fullsize2/PLOD1-Antibody-Western-Blot-NBP2-38770-img0002.jpg)
![Immunohistochemistry-Paraffin: PLOD1 Antibody [NBP2-38770] - Staining of human placenta shows cytoplasmic positivity in decidual cells.](http://images.novusbio.com/fullsize2/PLOD1-Antibody-Immunohistochemistry-Paraffin-NBP2-38770-img0003.jpg)
![Western Blot: CYP21A2 Antibody [NBP2-38698] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Adrenal Gland tissue](http://images.novusbio.com/fullsize2/CYP21A2-Antibody-Western-Blot-NBP2-38698-img0002.jpg)
![Immunohistochemistry-Paraffin: CYP21A2 Antibody [NBP2-38698] - Staining in human adrenal gland and pancreas tissues using anti-CYP21A2 antibody. Corresponding CYP21A2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/CYP21A2-Antibody-Immunohistochemistry-Paraffin-NBP2-38698-img0007.jpg)
![Immunohistochemistry-Paraffin: CYP21A2 Antibody [NBP2-38698] - Staining of human adrenal gland shows high expression.](http://images.novusbio.com/fullsize2/CYP21A2-Antibody-Immunohistochemistry-Paraffin-NBP2-38698-img0005.jpg)
![Western Blot: Beta Hydroxysteroid Dehydrogenase Antibody [NBP1-32353] - Sample(30 ug of whole cell lysate)A:HeLa S3 (GTX14654)B:MOLT4(GTX27912)12% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize2/Beta Hydroxysteroid Dehydrogenase Antibody-Western Blot-NBP1-32353-img0003.jpg)
![Immunohistochemistry: Beta Hydroxysteroid Dehydrogenase Antibody [NBP1-32353] - Paraffin-embedded normal liver; antibody dilution 1:500.](http://images.novusbio.com/fullsize2/Beta Hydroxysteroid Dehydrogenase Antibody-Immunohistochemistry-NBP1-32353-img0002.jpg)
![Western Blot: Ferredoxin Reductase Antibody [NBP2-38530] - Analysis in human cell lines A-549 and U-251MG using anti-FDXR antibody. Corresponding FDXR RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize2/Ferredoxin-Reductase-Antibody-Western-Blot-NBP2-38530-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Ferredoxin Reductase Antibody [NBP2-38530] - Staining of human cell line U-2 OS shows positivity in mitochondria.](http://images.novusbio.com/fullsize2/Ferredoxin-Reductase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38530-img0001.jpg)
![Immunohistochemistry-Paraffin: Ferredoxin Reductase Antibody [NBP2-38530] - Staining in human adrenal gland and pancreas tissues using anti-FDXR antibody. Corresponding FDXR RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/Ferredoxin-Reductase-Antibody-Immunohistochemistry-Paraffin-NBP2-38530-img0006.jpg)
![Western Blot: Androgen R/NR3C4 [p Ser213, p Ser210] Antibody (156C135.2) [NB100-56603] - Analysis using Azide Free version of NB100-56603. LNCaP cells (passage number 38) were serum-starved for 2 days. After serum starvation, cells were (A) left untreated, (B) treated with 100 ng/ml IGF-1 for 4h, or (C) incubated with 20 um LY294002 for 30 mi](http://images.novusbio.com/fullsize2/Androgen-R-NR3C4-[p-Ser213--p-Ser210]-Antibody-(156C135.2)-Western-Blot-NB100-56603-img0004.jpg)
![Western Blot: PBR Antibody [NB100-41398] - Western blot analysis of PBR in Human Breast Cancer lysate (35ug protein in RIPA buffer) using NB100-41398 at 0.1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize2/PBR-Antibody-Western-Blot-NB100-41398-img0006.jpg)
![Immunohistochemistry-Paraffin: PBR Antibody [NB100-41398] - Staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize2/PBR-Antibody-Immunohistochemistry-Paraffin-NB100-41398-img0007.jpg)
![Immunohistochemistry-Paraffin: PBR Antibody [NB100-41398] - IHC staining (2.5 ug/ml) of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize2/PBR-Antibody-Immunohistochemistry-Paraffin-NB100-41398-img0005.jpg)
![Western Blot: AKR1B1 Antibody [NBP1-89146] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize2/AKR1B1-Antibody-Western-Blot-NBP1-89146-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: AKR1B1 Antibody [NBP1-89146] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize2/AKR1B1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89146-img0006.jpg)
![Immunohistochemistry-Paraffin: AKR1B1 Antibody [NBP1-89146] - Staining in human adrenal gland and cerebral cortex tissues using anti-AKR1B1 antibody. Corresponding AKR1B1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/AKR1B1-Antibody-Immunohistochemistry-Paraffin-NBP1-89146-img0011.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - FLOW analysis of Mouse CD4+ T cells that were stimulated with anti-CD3/CD28 beads and insulin (1ug/mL) for 5 days in culture media with additional glucose provided. PE conjugated Glut1 antibody stained cells (NB110-39113PE, Orange) were included as positive control which were compared with Rb IgG PE, NBP2-24983 as Isotype Control (Blue) as well as with fluorescence minus one/FMO control (Red) [Image submitted by Verified Customer - see Review for Protocol Details]. Image using the PE form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0005.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - FLOW analysis of Mouse CD4+ T cells that were stimulated with anti-CD3/CD28 beads and insulin (1ug/mL) for 5 days in culture media with additional glucose provided. PE conjugated Glut1 antibody stained cells (NB110-39113PE, Orange) were included as positive control which were compared with Rb IgG PE, NBP2-24983 as Isotype Control (Blue) as well as with fluorescence minus one/FMO control (Red) [Image submitted by Verified Customer - see Review for Protocol Details]. Image using the PE form of this antibody.")