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CYP11A1 Antibody

 (2 Publications)
 (4 Reviews)
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Immunohistochemistry-Paraffin: CYP11A1 Antibody [NBP1-85368] - Staining in human adrenal gland and cerebral cortex tissues using anti-CYP11A1 antibody. Corresponding CYP11A1 RNA-seq data are presented for the same tissues.
Orthogonal Strategies: Immunohistochemistry-Paraffin: CYP11A1 Antibody [NBP1-85368] - Staining in human adrenal gland and cerebral cortex tissues using anti-CYP11A1 antibody. Corresponding CYP11A1 RNA-seq data ...read more
Western Blot: CYP11A1 Antibody [NBP1-85368] - protein extract from: 1) human liver (negative), 2) human testis (positve), 3) MW marker (drawn with special ECL reagent marker), 4) human testis, 5) H295R adrenal cell ...read more
Immunocytochemistry/ Immunofluorescence: CYP11A1 Antibody [NBP1-85368] - CYP11A1 expression (Leydig cells) present in early pubertal Rhesus monkey. Secondary antibody: Alexa Fluor488 anti rabbit. Image submitted by a ...read more
Immunohistochemistry-Paraffin: CYP11A1 Antibody [NBP1-85368] - Staining of human cerebral cortex shows low expression as expected.
Immunohistochemistry-Paraffin: CYP11A1 Antibody [NBP1-85368] - Staining of human adrenal gland shows high expression.

Product Details

Summary
Reactivity Hu, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

  

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CYP11A1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: RSVLVKGCQTFLSAPREGLGRLRVPTGEGAGISTRSPRPFNEIPSPGDNGWLNLYHFWRETGTHKVHLHHVQNFQKYGPIYREKLGNVESVYVIDPEDVALLFKSEGPNPERFLIPPWVAYHQYYQRPIGVLLKKSAA
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CYP11A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. CYP11A1 antibody validated for ICC/IF, WB from verified customer reviews.
Control Peptide
CYP11A1 Protein (NBP1-85368PEP)
Reviewed Applications
Read 4 Reviews rated 5
using
NBP1-85368 in the following applications:
Publications
Read Publications using NBP1-85368.

Reactivity Notes

Rhesus monkey reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CYP11A1 Antibody

  • Cholesterol desmolase
  • cholesterol monooxygenase (side-chain-cleaving)
  • cholesterol side-chain cleavage enzyme, mitochondrial
  • CYPXIA1
  • Cytochrome P450 11A1
  • Cytochrome P450(scc)
  • cytochrome P450, family 11, subfamily A, polypeptide 1
  • cytochrome P450C11A1
  • EC 1.14.15
  • EC 1.14.15.6
  • steroid 20-22-lyase
  • subfamily XIA (cholesterol side chain cleavage)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: StAR Antibody [NBP1-33485] - Human hepatoma. StAR antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Immunocytochemistry/Immunofluorescence: StAR Antibody [NBP1-33485] - HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: StAR protein stained by StAR antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Western Blot: StAR Antibody [NBP1-33485] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with StAR antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
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Immunohistochemistry: Cytochrome P450 17A1 Antibody (OTI3F11) [NBP2-01151] - Staining in 13 year old testis. Immunoexpression in Leydig cell. Image from a verified customer review.
Immunohistochemistry: Cytochrome P450 17A1 Antibody (OTI3F11) [NBP2-01151] - Staining in macaque Leydig cells. Image from a verified customer review.
Flow Cytometry: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Analysis of Hela cells, using anti-Cytochrome P450 17A1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
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Immunohistochemistry-Paraffin: SF-1/NR5A1/Steroidogenic Factor 1 Antibody (CL9101) [NBP2-88921] - Staining of human breast shows weak to moderate nuclear positivity in glandular cells.
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Applications: ICC/IF, IHC, IHC-P, WB
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Western Blot: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) and hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase. Image collected and cropped by CiteAb from the following publication (https://www.hindawi.com/journals/bmri/2017/3795950/), licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of hypothyroid (Hypo; solid bar; n = 6) rabbits. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of hypothyroid ovaries. Nonlabeling was observed when the primary antibody was omitted. Bars: 100 um (1st image); and 20 um (2nd image). Granulose cells, gc; theca cells, tc. Image collected and cropped by CiteAb from the following publication (https://www.hindawi.com/journals/bmri/2017/3795950/), licensed under a CC-BY licence.
(10 Publications)
Species: Hu, Mu, Pm, Rb, Rt
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
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Western Blot: POMC Antibody [NB100-1533] - Staining of NIH3T3 cell lysate with antibody at 0.3 ug/mL (A) and Rat Brain cell lysate with antibody at 2 ug/mL (B). 35 ug protein in RIPA buffer. Detected by chemiluminescence.
Flow Cytometry: POMC Antibody [NB100-1533] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunohistochemistry: POMC Antibody [NB100-1533] - Representative immunofluorescence images of (B) POMC as a prohormone (n = 5-6 each) in the ARC of mice approximately 5 to 10 weeks of age. Image collected and cropped by CiteAb from the following publication (jci.org/articles/view/96420), licensed under a CC-BY licence.
(10 Publications)
Species: Hu, Mu, Po, Rt, Sh
Applications: Flow, ICC/IF, IHC, IHC-P, PEP-ELISA, WB
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Western Blot: CYP11B1 Antibody [NBP1-68883] - Titration: 0.2-1 ug/ml, Positive Control: Human Liver.
Immunohistochemistry: CYP11B1 Antibody [NBP1-68883] - Monkey adrenal gland Primary Antibody Dilution: 1 : 25 Secondary Antibody: Anti-rabbit-HRP Secondary Antibody Dilution: 1 : 1000 Color/Signal Descriptions: Brown: CYP11B1 Blue: Nucleus Gene name: CYP11B1 Submitted by: Jonathan Bertin, Endoceutics Inc.
Immunohistochemistry: CYP11B1 Antibody [NBP1-68883] - Monkey vagina Primary Antibody Dilution: 1 : 25 Secondary Antibody: Anti-rabbit-HRP Secondary Antibody Dilution: 1 : 1000 Color/Signal Descriptions: Brown: CYP11B1 Blue: Nucleus Gene name: CYP11B1 Submitted by: Jonathan Bertin, Endoceutics Inc.
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Applications: IHC, IHC-P, WB
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Immunohistochemistry-Paraffin: BRD2 Antibody [NBP1-30475] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-BRD2 (NBP1-30475). Detection: DAB
Immunocytochemistry/Immunofluorescence: BRD2 Antibody [NBP1-30475] - Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti- BRD2 used at a dilution of 1:100 (2 ug/mL) (left) and rabbit anti-BRD2 recombinant monoclonal used at a dilution of 1:20 (right). Detection: Red-fluorescent goat anti-rabbit IgG cross-adsorbed Antibody DyLight 594 conjugated.
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(1 Review)
(1 Publication)
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(3 Publications)
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Immunohistochemistry-Paraffin: CYP21A2 Antibody [NBP2-13893] - Staining of human testis.
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Applications: IHC, IHC-P, WB
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Western Blot: Beta Hydroxysteroid Dehydrogenase Antibody [NBP1-32353] - Sample (30 ug of whole cell lysate)A:HeLa S3 B:MOLT4 12% SDS PAGE, antibody diluted at 1:500.
(1 Review)
(1 Publication)
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Western Blot: Ferredoxin Reductase Antibody [NBP2-38530] - Analysis using Anti-FDXR antibody NBP2-38530 (A) shows similar pattern to independent antibody NBP2-38706 (B).
Immunohistochemistry-Paraffin: Ferredoxin Reductase Antibody [NBP2-38530] - Staining of human adrenal gland shows strong granular cytoplasmic positivity in glandular cells.
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Applications: ICC/IF, IHC, IHC-P, WB
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Flow Cytometry: PBR Antibody [NB100-41398] - Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunocytochemistry/Immunofluorescence: PBR Antibody [NB100-41398] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative cLontrol: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody.
Immunocytochemistry/Immunofluorescence: PBR Antibody [NB100-41398] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
(12 Publications)
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Immunohistochemistry-Paraffin: AKR1B1 Antibody [NBP1-89146] - Staining of human cerebral cortex shows low expression as expected.
Immunohistochemistry-Paraffin: AKR1B1 Antibody [NBP1-89146] - Staining in human adrenal gland and cerebral cortex tissues using anti-AKR1B1 antibody. Corresponding AKR1B1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: AKR1B1 Antibody [NBP1-89146] - Analysis in human adrenal gland and liver tissues. Corresponding AKR1B1 RNA-seq data are presented for the same tissues.
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CYP11A1 Antibody
NBP1-85368
CYP11A1 Antibody
Species: Hu, RM
Applications: WB, ICC/IF, IHC, IHC-P

Publications for CYP11A1 Antibody (NBP1-85368)(2)

We have publications tested in 1 application: IHC.


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(1)
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Reviews for CYP11A1 Antibody (NBP1-85368) (4) 54

Average Rating: 5
(Based on 4 reviews)
We have 4 reviews tested in 2 species: Human, Rhesus Macaque.

Reviews using NBP1-85368:
Filter by Applications
Immunofluorescence-Paraffin
(1)
Immunohistochemistry-Paraffin
(2)
Western Blot
(1)
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(2)
Rhesus Macaque
(2)
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Images Ratings Applications Species Date Details
Other CYP11A1 NBP1-85368
Enlarge 		5
reviewed by:
Verified Customer
 Immunofluorescence-Paraffin Rhesus Macaque 03/09/2018
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Summary

ApplicationOther
Sample TestedEarly pubertal testis
SpeciesRhesus Macaque
LotB106749

Comments

CommentsPrimary antibody dilution 1:250.
Secondary antibody used: Alexa Fluor 488 anti rabbit
Immunohistochemistry-Paraffin CYP11A1 NBP1-85368
Enlarge 		5
reviewed by:
Verified Customer
 Immunohistochemistry-Paraffin Rhesus Macaque 11/24/2017
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedpubertal testis
SpeciesRhesus Macaque
LotB106749
Immunohistochemistry-Paraffin CYP11A1 NBP1-85368
Enlarge 		5
reviewed by:
Verified Customer
 Immunohistochemistry-Paraffin Human 10/09/2017
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman testis
SpeciesHuman
LotB106749

Comments

CommentsAntibody diluted 1/250
Western Blot CYP11A1 NBP1-85368
Enlarge 		5
reviewed by:
Verified Customer
 Western Blot Human 10/09/2017
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Summary

ApplicationWestern Blot
Sample TestedHuman liver,Human testis,Whole cell lysate and mitochondrial extract of H295R cells (human adrenal cortical cells)
SpeciesHuman
LotB106749

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FAQs for CYP11A1 Antibody (NBP1-85368) (0)

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Recent Reviews

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Other CYP11A1 NBP1-85368
Enlarge
 Verified Customer
03/09/2018
Application: Immunofluorescence-Paraffin
Species: Rhesus Macaque
read this review
Immunohistochemistry-Paraffin CYP11A1 NBP1-85368
Enlarge
 Verified Customer
11/24/2017
Application: Immunohistochemistry-Paraffin
Species: Rhesus Macaque
read this review
Immunohistochemistry-Paraffin CYP11A1 NBP1-85368
Enlarge
 Verified Customer
10/09/2017
Application: Immunohistochemistry-Paraffin
Species: Human
read this review

Bioinformatics

Gene Symbol CYP11A1