APE Antibody


Western Blot: APE Antibody [NB100-101] - SH-SY5Y (nuclear extract) tested at 1:1000 dilution. Image provided by verified customer review.
Immunocytochemistry/ Immunofluorescence: APE Antibody [NB100-101] - IF on HeLa cells. Image from verified customer review.
Immunohistochemistry: APE Antibody [NB100-101] - Immunohistochemical staining of APE-ref-1 in prostate cancer.
Western Blot: APE Antibody [NB100-101] - analysis of APE in human melanoma cell lysate using anti-APE antibody. Image from verified customer review.
Western Blot: APE Antibody [NB100-101] - Analysis of APE1 in cell lysates: 1. HeLa, 2. Ntera2, 3. A431, 4. HepG2, 5. MCF7, 6. NIH 3T3, 7. PC12, and 8. Cos 7.
Immunocytochemistry/ Immunofluorescence: APE Antibody [NB100-101] - Detection of APE1 (Green) in HepG2 cells using NB100-101. Nuclei (Blue) are counterstained with Hoechst 33258.
Simple Western: APE Antibody [NB100-101] - Simple Western lane view shows a specific band for APE1 in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more

Product Details

Reactivity Hu, Mu, Rt, Pm, RbSpecies Glossary
Applications WB, Simple Western, B/N, ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, IP
0.5 mg/ml

APE Antibody Summary

Affinity purified human APE1 [UniProt# P27695]
In immunohistochemical experiments punctate nuclear staining is seen in most cell types. However, in hepatocytes, large neurons and granule cells, cytoplasmic staining is observed.
Immunogen affinity purified
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Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Tris-glycine, 150 mM NaCl
0.05% Sodium Azide
0.5 mg/ml
Immunogen affinity purified


  • Western Blot 1:1000
  • Simple Western 1:12.5
  • Chromatin Immunoprecipitation 1:10-1:500
  • Blocking/Neutralizing 50-200 molar excess
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 7 ug/ml
Application Notes
This APE1 antibody is useful for Blocking/Neutralizing, Chromatin Immunoprecipitation, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Western Blot. In WB this antibody detects a single band at 37 kDa. In IHC it can be competitively inhibited from recognizing the APE1 antigen in tissues using APE1 protein. This antibody can be used on frozen sections, fixed-paraffin sections and cytospin preps. NB100-101 can also be used following the apoptosis (TUNEL) procedure with the Boehringer-Mannheim TUNEL assay kit. Antibody staining should be performed AFTER the TUNEL assay. NB100-101 can inhibit the repair activity of APE1 protein.In Simple Western only 10-15 uL of the recommended dilution is used per data point. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
APE Protein (NB100-101PEP)
Reviewed Applications
Read 3 Reviews rated 5
NB100-101 in the following applications:

Read Publications using
NB100-101 in the following applications:

  • 1 publication
  • 5 publications
  • IHC
    8 publications
  • 4 publications
  • IP
    1 publication
  • WB
    33 publications

Reactivity Notes

Rabbit reactivity reported in the scientific literature (PMID: 15276530).


SH-SY5Y Lysate (nuclear extract) image in western blot provided via veririfed customer review.

Alternate Names for APE Antibody

  • AP endonuclease 1
  • APE
  • APE1
  • APE-1
  • APEAPEX nuclease (multifunctional DNA repair enzyme)
  • APENAP endonuclease class I
  • APEX deoxyribonuclease (apurinic or apyrimidinic)
  • APEX nuclease (multifunctional DNA repair enzyme) 1
  • APEX nuclease
  • APEX1
  • Apurinic-apyrimidinic endonuclease 1
  • APXAP lyase
  • DNA-(apurinic or apyrimidinic site) lyase
  • EC 3.1
  • EC
  • HAP1apurinic/apyrimidinic (abasic) endonuclease
  • multifunctional DNA repair enzyme
  • protein REF-1
  • redox factor 1
  • Redox factor-1
  • REF1 apurinic/apyrimidinic exonuclease
  • REF-1


APE1 (apurinic-apyrimidinic endonuclease 1) belongs to DNA repair enzymes AP/ExoA family and plays a key role in cellular response to oxidative stress, DNA repair and redox regulation of transcriptional factors. APE1 functions as apurinic/apyrimidinic (AP) endodeoxyribonuclease in DNA base excision repair (BER) pathway of DNA lesions induced by oxidative stress, alkylating agents, and ionizing radiation. It participates in DNA demethylation for epigenetic regulation of gene expression and delivers endoribonuclease activity for controlling single-stranded RNA metabolism. APE1 acts as a loading factor for POLB onto non-incised AP sites in DNA for stimulating the 5'-terminal dRp-excision activity of POLB and involve in DNA cleavage step of class switch recombination. Together with HNRNPL or XRCC5/XRCC6 dimer, APE1 associates with nCaRE to acting as an activator of transcriptional repression. When acetylated at Lys-6 and Lys-7, APE1 stimulates YBX1-mediated MDR1 promoter activity resulting in drug resistance. Ape1 functions as a redox factor that maintains transcription factors in an active, reduced state and also function in a redox-independent manner as a transcriptional cofactor to control different cellular fates such as apoptosis, proliferation and differentiation. APE1 stimulates DNA binding of several transcription factors known to be involved in tumor progression such as Fos, Jun, NF-kB, PAX, HIF-1, HLF and p53. Increased APE1 expression is associated with many types of cancers including cervical, ovarian, prostate, rhabdomyosarcomas and germ cell tumors, and APE1 mutation has also been associated with amyotrophic lateral sclerosis (ALS).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for APE Antibody (NB100-101)(55)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Rabbit.

We have publications tested in 6 applications: ChIP, ICC/IF, IHC, IHC-P, IP, WB.

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Showing Publications 1 - 10 of 55. Show All 55 Publications.
Publications using NB100-101 Applications Species
Nassour H, Wang Z, Saad A et al. Peroxiredoxin 1 interacts with and blocks the redox factor APE1 from activating interleukin-8 expression Sci Rep 2016 Jul 08 [PMID:27388124]
Poletto M, Legrand AJ, Fletcher SC et al. p53 coordinates base excision repair to prevent genomic instability. Nucleic Acids Res. 2016 Jan 14 [PMID:26773055] (WB, Human) WB Human
Jason L. Quinones,Upasna Thapar,Kefei Yu et al. Enzyme mechanism-based, oxidative DNA-protein cross-links formed with DNA polymerase beta in vivo. NCBI. 2015 Jul 14 [PMID:26124145] (WB, Mouse, Human) WB Mouse, Human
Iglesias-Ara A, Zenarruzabeitia O, Buelta L et al. E2F1 and E2F2 prevent replicative stress and subsequent p53-dependent organ involution Cell Death Differ. 2015 Feb 06 [PMID:25656653] (WB, Mouse) WB Mouse
Das D, Preet R, Mohapatra P et al. 5-Fluorouracil mediated anti-cancer activity in colon cancer cells is through the induction of Adenomatous Polyposis Coli: Implication of the long-patch base excision repair pathway. DnA Repair (Amst.). 2014 Nov 20 [PMID:25460919] (WB, Human) WB Human
Abdel-Fatah TM, Perry C, Arora A et al. Is there a role for base excision repair in estrogen driven breast cancers? Antioxid. Redox Signal. 2014 Aug 11 [PMID:25111287] (IHC-P, Human) IHC-P Human
Liu GD, Xia L, Zhu JW et al. Genistein Alleviates Radiation-Induced Pneumonitis by Depressing Ape1/Ref-1 Expression to Down-regulate Inflammatory Cytokines. Cell Biochem. Biophys. 3/23/2014 [PMID:24659138] (WB, Mouse) WB Mouse
Abdel-Fatah TM, Perry C, Moseley P et al. Clinicopathological significance of human apurinic/apyrimidinic endonuclease 1 (APE1) expression in oestrogen-receptor-positive breast cancer. Breast Cancer Res Treat 2014 Jan 1 [PMID:24381055] (IHC-P, Human) IHC-P Human
Alagoz M, Wells OS, El-Khamisy SF. TDP1 deficiency sensitizes human cells to base damage via distinct topoisomerase I and PARP mechanisms with potential applications for cancer therapy. Nucleic Acids Res 2013 Dec 13 [PMID:24335147] (WB, Human) WB Human
Das D, Preet R, Mohapatra P et al. 1,3-Bis(2-chloroethyl)-1-nitrosourea enhances the inhibitory effect of Resveratrol on 5-fluorouracil sensitive/resistant colon cancer cells. World J Gastroenterol. 2013 Nov 14 [PMID:24259968] (WB, Human) WB Human
Show All 55 Publications.

Reviews for APE Antibody (NB100-101) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.
We have 3 reviews tested in 2 application: WB, IF.

Reviews using NB100-101:
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Images Ratings Applications Species Date Details
Western Blot APE NB100-101
reviewed by:
WB Human 11/20/2014


ApplicationWestern Blot
Sample TestedMelanoma whole cell lysate


Blocking Details5% milk for 1 hour

Primary Anitbody

Dilution Ratio1:5000

Secondary Antibody

Secondary Descriptiongoat anti-rabbit IgG-HRP
Secondary Manufacturer Cat#sc-2004
Secondary Concentration1:5000


Detection NotesExposure time - 30 seconds
Immunofluorescence APE NB100-101
reviewed by:
IF Human 07/31/2014


Sample TestedHeLa cells


Blocking Details2% BSA in PBS, 1 h at RT

Primary Anitbody

Dilution Ratio1:2000 in 2% BSA/PBS, 14h at 4C

Secondary Antibody

Secondary DescriptionAlexa Fluor® 488 Goat Anti-Rabbit IgG (H+L)
Secondary Manufacturer Cat#A-11008 from Life Technologies (by Molecular Probes)
Secondary Concentration0.2 ug/ml


Fixation Details4% Paraformaldehyde as fixative, permeabilization with 0.2% Triton-X100
Wash Description3x washes in PBS for 5 min
Western Blot APE NB100-101
reviewed by:
WB Human 02/28/2014


ApplicationWestern Blot
Sample TestedSH-SY5Y lysate (nuclear extract)


Blocking Details5% milk in PBS, 0.2% Tween-20, 1 hr

Primary Anitbody

Dilution Ratio1:1,000 in 2.5% milk in PBS, o/n at 4 oC

Secondary Antibody

Secondary DescriptionGt anti-rabbit IgG-HRP
Secondary Manufacturer Cat#GE Healthcare
Secondary Concentration1:10,000


Detection NotesECL, Luminata Forte Reagent (EMD Millipore), 10 min exposure on G-Box imager (Syngene)

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for APE Antibody (NB100-101) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB100-101AF405
Alexa Fluor (R) 488 Labeled NB100-101AF488
Alexa Fluor (R) 647 Labeled NB100-101AF647
Alexa Fluor (R) 700 Labeled NB100-101AF700
Biotin Labeled NB100-101B
DyLight 405 Labeled NB100-101V
DyLight 488 Labeled NB100-101G
DyLight 550 Labeled NB100-101R
DyLight 650 Labeled NB100-101C
FITC Labeled NB100-101F
HRP Labeled NB100-101H

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Contact Information

Product PDFs


Gene Symbol APEX1

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