Glutathione Reductase Antibody

Western Blot: Glutathione Reductase Antibody [NBP2-16684] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutathione Reductase antibody [N2C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: Glutathione Reductase Antibody [NBP2-16684] - Rat brain. Glutathione reductase antibody [N2C2], Internal diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: Glutathione Reductase Antibody [NBP2-16684] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutathione reductase diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: Glutathione Reductase Antibody [NBP2-16684] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutathione reductase diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: Glutathione Reductase Antibody [NBP2-16684] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutathione Reductase antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: Glutathione Reductase Antibody [NBP2-16684] - Rat brain. Glutathione reductase antibody [N2C2], Internal diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Glutathione Reductase Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Glutathione reductase. The exact sequence is proprietary.
• Localization: Mitochondrion; Cytoplasm
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GSR
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 56 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Bovine (84%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol (pH7).
• Preservative: 0.025% Proclin 300
• Purity: Immunogen affinity purified

Alternate Names for Glutathione Reductase Antibody

• EC 1.8.1
• EC 1.8.1.7
• GLUR
• Glutathione Reductase
• glutathione reductase, mitochondrial
• GR
• GRase
• GRD1
• GSR
• HEL-752
• MGC78522

Background

This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. This enzyme is a homodimeric flavoprotein. It is a central enzyme of cellular antioxidant defense, and reduces oxidized glutathione disulfide (GSSG) to the sulfhydryl form GSH, which is an important cellular antioxidant. Rare mutations in this gene result in hereditary glutathione reductase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Glutathione Reductase Antibody (NBP2-16684)(2)

Publications using NBP2-16684

• Petrillo S, D’Amico J, Nicita F et al. Antioxidant Response in Human X-Linked Adrenoleukodystrophy Fibroblasts Antioxidants 2022-10-28 [PMID: 36358497] (WB, Human)
• Deka B, Barge SR, Bharadwaj S et al. Beneficial effect of the methanolic leaf extract of Allium hookeri on stimulating glutathione biosynthesis and preventing impaired glucose metabolism in type 2 diabetes Archives of biochemistry and biophysics 2021-09-15 [PMID: 34118216]

Bioinformatics

• Gene Symbol: GSR
• Uniprot:
  • P00390()