APE Antibody [FITC]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, RbSpecies Glossary
Applications ICC/IF, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
FITC

APE Antibody [FITC] Summary

Immunogen
Affinity purified human APE1 [UniProt# P27695]
Localization
In immunohistochemical experiments punctate nuclear staining is seen in most cell types. However, in hepatocytes, large neurons and granule cells, cytoplasmic staining is observed.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
APEX1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
This APE1 antibody is useful for Blocking/Neutralizing, Chromatin Immunoprecipitation, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Western Blot. In WB this antibody detects a single band at 37 kDa. In IHC it can be competitively inhibited from recognizing the APE1 antigen in tissues using APE1 protein. This antibody can be used on frozen sections, fixed-paraffin sections and cytospin preps. NB100-101 can also be used following the apoptosis (TUNEL) procedure with the Boehringer-Mannheim TUNEL assay kit. Antibody staining should be performed AFTER the TUNEL assay. NB100-101 can inhibit the repair activity of APE1 protein.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Antagonist

Reactivity Notes

Rabbit reactivity reported in the scientific literature (PMID: 15276530).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for APE Antibody [FITC]

  • AP endonuclease 1
  • APE
  • APE1
  • APE-1
  • APEAPEX nuclease (multifunctional DNA repair enzyme)
  • APENAP endonuclease class I
  • APEX deoxyribonuclease (apurinic or apyrimidinic)
  • APEX nuclease (multifunctional DNA repair enzyme) 1
  • APEX nuclease
  • APEX1
  • Apurinic-apyrimidinic endonuclease 1
  • APXAP lyase
  • DNA-(apurinic or apyrimidinic site) lyase
  • EC 3.1
  • EC 4.2.99.18
  • HAP1apurinic/apyrimidinic (abasic) endonuclease
  • multifunctional DNA repair enzyme
  • protein REF-1
  • redox factor 1
  • Redox factor-1
  • REF1 apurinic/apyrimidinic exonuclease
  • REF-1

Background

APE1 (apurinic-apyrimidinic endonuclease 1) belongs to DNA repair enzymes AP/ExoA family and plays a key role in cellular response to oxidative stress, DNA repair and redox regulation of transcriptional factors. APE1 functions as apurinic/apyrimidinic (AP) endodeoxyribonuclease in DNA base excision repair (BER) pathway of DNA lesions induced by oxidative stress, alkylating agents, and ionizing radiation. It participates in DNA demethylation for epigenetic regulation of gene expression and delivers endoribonuclease activity for controlling single-stranded RNA metabolism. APE1 acts as a loading factor for POLB onto non-incised AP sites in DNA for stimulating the 5'-terminal dRp-excision activity of POLB and involve in DNA cleavage step of class switch recombination. Together with HNRNPL or XRCC5/XRCC6 dimer, APE1 associates with nCaRE to acting as an activator of transcriptional repression. When acetylated at Lys-6 and Lys-7, APE1 stimulates YBX1-mediated MDR1 promoter activity resulting in drug resistance. Ape1 functions as a redox factor that maintains transcription factors in an active, reduced state and also function in a redox-independent manner as a transcriptional cofactor to control different cellular fates such as apoptosis, proliferation and differentiation. APE1 stimulates DNA binding of several transcription factors known to be involved in tumor progression such as Fos, Jun, NF-kB, PAX, HIF-1, HLF and p53. Increased APE1 expression is associated with many types of cancers including cervical, ovarian, prostate, rhabdomyosarcomas and germ cell tumors, and APE1 mutation has also been associated with amyotrophic lateral sclerosis (ALS).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol APEX1
Entrez