APE Antibody [Alexa Fluor™ Plus 680]

• Reactivity: Hu, Mu, Rt, Pm, Rb
• Applications: WB, Flow, ICC/IF, IHC, IP, B/N, ChIP, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Alexa Fluor Plus 680

APE Antibody [Alexa Fluor™ Plus 680] Summary

• Immunogen: Affinity purified human APE1 [UniProt# P27695]
• Localization: In immunohistochemical experiments punctate nuclear staining is seen in most cell types. However, in hepatocytes, large neurons and granule cells, cytoplasmic staining is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: APEX1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • Chromatin Immunoprecipitation (ChIP)
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Rabbit reactivity reported in the scientific literature (PMID: 15276530).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for APE Antibody [Alexa Fluor™ Plus 680]

• AP endonuclease 1
• APE
• APE1
• APE-1
• APEAPEX nuclease (multifunctional DNA repair enzyme)
• APENAP endonuclease class I
• APEX deoxyribonuclease (apurinic or apyrimidinic)
• APEX nuclease (multifunctional DNA repair enzyme) 1
• APEX nuclease
• APEX1
• Apurinic-apyrimidinic endonuclease 1
• APXAP lyase
• DNA-(apurinic or apyrimidinic site) lyase
• EC 3.1
• EC 4.2.99.18
• HAP1apurinic/apyrimidinic (abasic) endonuclease
• multifunctional DNA repair enzyme
• protein REF-1
• redox factor 1
• Redox factor-1
• REF1 apurinic/apyrimidinic exonuclease
• REF-1

Background

DNA-(apurinic or apyrimidinic site) lyase, also known as APE1 or APEX1, is a multifunctional mammalian proteins responsible for the cellular response to oxidative stress. Specifically, APE-1 both functions in the DNA base excision repair pathway and regulates the redox of transcriptional factors.

For DNA repair, APE1 functions as a mammalian apurinic/apyrimidinic (AP) endodeoxyribonuclease responsible for the repair of AP sites in DNA lesions induced by oxidative and alkylating agents.

APEX1 also exerts a reversible nuclear redox activity to regulate the DNA binding affinity and the activity of transcriptional factors by regulating the redox status of their DNA-binding domains.

APE1 antibodies are useful for studies on both DNA repair and transcriptional regulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: APEX1