APE Antibody [DyLight 405]


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Product Details

Reactivity Hu, Mu, Rt, Pm, RbSpecies Glossary
Applications WB, ICC/IF, IHC-Fr, IHC-P
DyLight 405

APE Antibody [DyLight 405] Summary

Affinity purified human APE1 [UniProt# P27695]
In immunohistochemical experiments punctate nuclear staining is seen in most cell types. However, in hepatocytes, large neurons and granule cells, cytoplasmic staining is observed.
Immunogen affinity purified
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  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
This APE1 antibody is useful for Blocking/Neutralizing, Chromatin Immunoprecipitation, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Western Blot. In WB this antibody detects a single band at 37 kDa. In IHC it can be competitively inhibited from recognizing the APE1 antigen in tissues using APE1 protein. This antibody can be used on frozen sections, fixed-paraffin sections and cytospin preps. NB100-101 can also be used following the apoptosis (TUNEL) procedure with the Boehringer-Mannheim TUNEL assay kit. Antibody staining should be performed AFTER the TUNEL assay. NB100-101 can inhibit the repair activity of APE1 protein.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rabbit reactivity reported in the scientific literature (PMID: 15276530).

Packaging, Storage & Formulations

Store at 4C in the dark.
50mM Sodium Borate
0.05% Sodium Azide
Immunogen affinity purified


Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for APE Antibody [DyLight 405]

  • AP endonuclease 1
  • APE
  • APE1
  • APE-1
  • APEAPEX nuclease (multifunctional DNA repair enzyme)
  • APENAP endonuclease class I
  • APEX deoxyribonuclease (apurinic or apyrimidinic)
  • APEX nuclease (multifunctional DNA repair enzyme) 1
  • APEX nuclease
  • APEX1
  • Apurinic-apyrimidinic endonuclease 1
  • APXAP lyase
  • DNA-(apurinic or apyrimidinic site) lyase
  • EC 3.1
  • EC
  • HAP1apurinic/apyrimidinic (abasic) endonuclease
  • multifunctional DNA repair enzyme
  • protein REF-1
  • redox factor 1
  • Redox factor-1
  • REF1 apurinic/apyrimidinic exonuclease
  • REF-1


APE1 (apurinic-apyrimidinic endonuclease 1) belongs to DNA repair enzymes AP/ExoA family and plays a key role in cellular response to oxidative stress, DNA repair and redox regulation of transcriptional factors. APE1 functions as apurinic/apyrimidinic (AP) endodeoxyribonuclease in DNA base excision repair (BER) pathway of DNA lesions induced by oxidative stress, alkylating agents, and ionizing radiation. It participates in DNA demethylation for epigenetic regulation of gene expression and delivers endoribonuclease activity for controlling single-stranded RNA metabolism. APE1 acts as a loading factor for POLB onto non-incised AP sites in DNA for stimulating the 5'-terminal dRp-excision activity of POLB and involve in DNA cleavage step of class switch recombination. Together with HNRNPL or XRCC5/XRCC6 dimer, APE1 associates with nCaRE to acting as an activator of transcriptional repression. When acetylated at Lys-6 and Lys-7, APE1 stimulates YBX1-mediated MDR1 promoter activity resulting in drug resistance. Ape1 functions as a redox factor that maintains transcription factors in an active, reduced state and also function in a redox-independent manner as a transcriptional cofactor to control different cellular fates such as apoptosis, proliferation and differentiation. APE1 stimulates DNA binding of several transcription factors known to be involved in tumor progression such as Fos, Jun, NF-kB, PAX, HIF-1, HLF and p53. Increased APE1 expression is associated with many types of cancers including cervical, ovarian, prostate, rhabdomyosarcomas and germ cell tumors, and APE1 mutation has also been associated with amyotrophic lateral sclerosis (ALS).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies


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Gene Symbol APEX1