Orthogonal Strategies: Immunohistochemistry-Paraffin: DNA Polymerase beta Antibody [NBP2-38600] - Staining in human testis and liver tissues using anti-POLB antibody. Corresponding POLB RNA-seq data are presented ...read more
Western Blot: DNA Polymerase beta Antibody [NBP2-38600] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4
Immunocytochemistry/ Immunofluorescence: DNA Polymerase beta Antibody [NBP2-38600] - Staining of human cell line A-431 shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: DNA Polymerase beta Antibody [NBP2-38600] - Staining of human liver shows low expression as expected.
Immunohistochemistry: DNA Polymerase beta Antibody [NBP2-38600] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.2) and 40% Glycerol
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for DNA Polymerase beta Antibody
DNA pol beta
DNA polymerase beta subunit
DNA Polymerase beta
polymerase (DNA directed), beta
DNA polymerase beta comprises an amino terminal 8kDa domain and a carboxy terminal 31kDa domain. DNA Polymerase beta, a DNA repair polymerase is constitutively expressed in cultured cells. DNA polymerase beta fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Genomic DNA samples from LN428 cells -treated by copper complex to induce DNA-protein cross-links
After the rabbit anti-DNA polymerase beta antibody from Novus Biologicals NB100-91734, which this lab has been using for DPC assay, being discontinued by Novus biologicals, we have been looking for an available antibody for the application of DPC assay.
LN428 human cancer cells were treated by 100 uM of 1,10-Phenanthroline-Copper (II) complex to induce genomic DNA damages (DNA-protein cross-links); a serial of amount of gDNA samples (0, 200, 500, 1000 and 2000ng) were prepared and gDNA samples were blotted onto nitrocellulose membrane and probed for DNA polymerase beta which was trapped on the sites of gDNA damages.
The NBP2-38600 antibody was compared with the discontinued DNA Polymerase beta antibody NB100-91734, which we have been using and has been working very well for DPC assay,
The result showed that NBP2-38600 worked as good as, or evenbetter than, the discontinued NB100-91734.
Slot blot DPC assay, a new and widely-used technique to detect DNA-Protein Cross-links (DPC) - induced by oxidative stresses in vitro (in tubes) or in vivo (in cells).
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