OGG1 Antibody

Images

 
Western Blot: OGG1 Antibody [NB100-106] - Analysis of Ogg1 recombinant protein using Ogg1 antibody at 1 ug/mL.
Immunocytochemistry/ Immunofluorescence: OGG1 Antibody [NB100-106] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated ...read more
Immunohistochemistry-Paraffin: OGG1 Antibody [NB100-106] - Analysis of OGG1 in human skin tissues. Image courtesy of product review by Arash Javeri.
Flow Cytometry: OGG1 Antibody [NB100-106] - An intracellular stain was performed on Jurkat Cells with OGG1 Antibody NB100-106 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% ...read more
Western Blot: OGG1 Antibody [NB100-106] - - Analysis of Ogg1 expression in Jurkat whole cell lysate.
Western Blot: OGG1 Antibody [NB100-106] - 1, 5, 10 and 20 ng titration of human recombinant Ogg1 protein, detected by NB 100-106.
Immunohistochemistry-Frozen: OGG1 Antibody [NB100-106] - Detection of OGG1 in formaldehyde-fixed frozen sections of the substantia nigra from a Rhesus macaque (Macaca mulatta) using NB100-106 at 10 ug/mL. Photo courtesy ...read more
Immunohistochemistry: OGG1 Antibody [NB100-106] - Staining of human tonsil, germinal center and mantle zone.
Flow Cytometry: OGG1 Antibody [NB100-106] - Baseline Ogg1 expression in human PBMC from a healthy donor. Image from verified.customer review.
Flow (Intracellular): OGG1 Antibody [NB100-106] - An intracellular stain was performed on HeLa cells with NB100-106C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, RbSpecies Glossary
Applications WB, ELISA, Flow, Flow-IC, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

OGG1 Antibody Summary

Immunogen
A peptide derived from human Ogg1 (within amino acids 1-100). [UniProt# O15527]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
OGG1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • ELISA
  • Flow Cytometry
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:100 - 1:200
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:25-1:100
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:10-1:500
  • Flow (Intracellular)
Application Notes
Use in Immunoprecipitation Co-IP (PMID: 20956573) and ELISA (PMID: 19506022) has been reported in the literature. Use in Immunoblotting reported in multiple pieces of scientific literature. In WB, it recognizes a band at ~39 kDa, representing Ogg1. Flow data from customer review. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Jurkat Whole Cell Lysate
OGG1 Knockout HeLa Cell Lysate
Control Peptide
Reviewed Applications
Read 4 Reviews rated 4.5
using
NB100-106 in the following applications:

Publications
Read Publications using
NB100-106 in the following applications:

  • 1 publication
  • IB
    4 publications
  • 9 publications
  • IHC
    11 publications
  • 2 publications
  • 2 publications
  • IP
    3 publications
  • WB
    41 publications

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 16651612).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for OGG1 Antibody

  • 8-hydroxyguanine DNA glycosylase
  • 8-oxoguanine DNA glycosylase
  • AP lyase
  • DNA-apurinic or apyrimidinic site lyase
  • HOGG1
  • MMH
  • MUTMOGH1HMMH
  • N-glycosylase/DNA lyase
  • OGG1

Background

8-hydroxyguanine, a form of oxidative DNA damage induced by free radicals, causes G:C to T:A transversion. In E. coli, three DNA repair enzymes exist to prevent the mutagenic effects of 8-hydroxyguanine. One of these enzymes, MutM, was found to have a functional yeast (yOgg1) and human (hOgg1) homologue. hOgg1 proteins efficiently release the 8-hydroxyguanine opposite the pyrimidine from DNA and cleave the AP site in a manner similar to bacterial and yeast enzymes. Genetic backgrounds in control of the repair of damaged DNA are involved in the susceptibility to cancer development. The hOgg1 gene has been mapped to region 3p26.2, a region showing loss of heterozygosity (LOH) in a variety of cancers. In particular, 3p25-p26 is a common LOH region in lung cancer. Recent work has demonstrated that Ogg plays an important role in CAG expansion, a characteristic of several neurodegenerative diseases. Ogg appears to be responsible for progressive expansion of poly-Q tracts in response to oxidative damage. Thus, Ogg provides a direct link between DNA damage and toxicity in neurons.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for OGG1 Antibody (NB100-106)(96)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Primate, Rabbit.

We have publications tested in 8 applications: ELISA, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB.


Filter By Application
ELISA
(1)
IB
(4)
ICC/IF
(9)
IHC
(11)
IHC-Fr
(2)
IHC-P
(2)
IP
(3)
WB
(41)
All Applications
Filter By Species
Human
(37)
Mouse
(13)
Rat
(16)
Primate
(1)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 96. Show All 96 Publications.
Publications using NB100-106 Applications Species
Lorenzo Y, Haug Berg K, Ringvold A et al. Levels of oxidative DNA damage are low in exvivo engineered human limbal epithelial tissue. Acta Ophthalmol Sep 21 2018 [PMID: 30239138] (IHC, Human) IHC Human
Rahmani Yeganeh P. The impact of apple peel polyphenols on intestinal and mitochondrial functions in experimental colitis Thesis 2017 (Mouse) Mouse
Fouquerel E, Barnes RP, Uttam S et al. Targeted and Persistent 8-Oxoguanine Base Damage at Telomeres Promotes Telomere Loss and Crisis Mol. Cell May 10 2019 [PMID: 31101499]
Aiken CE, Tarry-Adkins JL, Spiroski AM et al. Chronic fetal hypoxia disrupts the peri-conceptual environment in next-generation adult female rats J. Physiol. (Lond.) Feb 21 2019 [PMID: 30791124] (WB, Rat) WB Rat
Kumagae Y, Hirahashi M, Takizawa K et al. Overexpression of MTH1 and OGG1 proteins in ulcerative colitis-associated carcinogenesis Oncol Lett May 25 2018 [PMID: 30008864] (IHC, Human) IHC Human
Kleme ML, Sane A, Garofalo C et al. CFTR Deletion Confers Mitochondrial Dysfunction and Disrupts Lipid Homeostasis in Intestinal Epithelial Cells. Nutrients Jun 27 2018 [PMID: 29954133] (WB, Human) WB Human
Sony S. Oxidative damage to dna in alzheimers disease. PhD Theses -Chemistry 2013 (WB, Human) WB Human
Aiken CE, Tarry-Adkins JL, Spiroski AM et al. Chronic gestational hypoxia accelerates ovarian aging and lowers ovarian reserve in next-generation adult rats FASEB J. Mar 19 2019 [PMID: 30888848] (WB, Rat) WB Rat
Iida R, Ueki M, Yasuda T. knockout of Mpv17-Like Protein (M-LPH) Gene in Human Hepatoma Cells Results in Impairment of mtDNA Integrity through Reduction of TFAM, OGG1, and LIG3 at the Protein Levels. Oxid Med Cell Longev. Sep 17 2018 [PMID: 30310528] (WB, Human) WB Human
Rivera-Barahona A, Alonso-Barroso E, Perez B et al. Treatment with antioxidants ameliorates oxidative damage in a mouse model of propionic acidemia. Mol. Genet. Metab. 2017 Jul 25 [PMID: 28774709] (Mouse) Mouse
Show All 96 Publications.

Reviews for OGG1 Antibody (NB100-106) (4) 4.54

Average Rating: 4.5
(Based on 4 reviews)
We have 4 reviews tested in 1 species: Human.

Reviews using NB100-106:
Filter by Applications
IP
(1)
Flow
(1)
WB
(1)
IHC
(1)
All Applications
Filter by Species
Human
(4)
All Species
Images Ratings Applications Species Date Details
Immunoprecipitation OGG1 NB100-106
Enlarge
reviewed by:
TIRZAH LAJUS
IP Human 11/25/2014
View

Summary

ApplicationImmunoprecipitation
Sample TestedMRC5 WCE- IP with Ogg1 ab and immunoblot with Ogg1 ab
SpeciesHuman

Blocking

Blocking Details5% milk in TBS tween 0,1%, 1h @ RT

Secondary Antibody

Secondary Descriptiongoat-anti-mouse HRP

Details

Detection NotesDetection using ECL from Amersham and Kodak films
Immunoprecipitation DetailsIP Gbeads, incubation with ab o/n @4C, 3x wash with elution buffer
Flow Cytometry OGG1 NB100-106
Enlarge
reviewed by:
Anonymous
Flow Human 06/30/2014
View

Summary

ApplicationFlow Cytometry
Sample TestedHuman PBMC
SpeciesHuman

Primary Anitbody

Dilution Ratio1:100 in BD Perm/Wash Buffer
Western Blot OGG1 NB100-106
Enlarge
reviewed by:
Saniya Fayzullina
WB Human 11/12/2012
View

Summary

ApplicationWestern Blot
Sample Testedhuman recombinant Ogg1, mouse skeletal muscle whole lysate, mouse spinal cord whole lysate
SpeciesHuman
CommentsThis antibody worked on western blot on human recombinant protein, but not on mouse whole protein lysates from skeletal muscle and spinal cord. It was clean using milk blocking buffer. It was very dirty using BSA blocking buffer.

Blocking

Blocking Details2.5% Milk, 0.1% Tween80 blocking buffer 2x30minutes at room temperature

Primary Anitbody

Dilution Ratio1:2000 in milk blocking buffer, 4C overnight

Secondary Antibody

Secondary Descriptiongoat anti-rabbit IgG, HRP (Invitrogen)
Secondary Concentration1:10000 in milk blocking buffer

Details

Detection NotesECL, film exposed for 1 hour

Comments

CommentsThis antibody worked on western blot on human recombinant protein, but not on mouse whole protein lysates from skeletal muscle and spinal cord. It was clean using milk blocking buffer. It was very dirty using BSA blocking buffer.
Immunohistochemistry OGG1 NB100-106
Enlarge
reviewed by:
Anonymous
IHC Human 05/17/2011
View

Summary

ApplicationImmunohistochemistry
Sample TestedHuman foreskin, adult ski, engineered human skin, keratinocytes, HaCaT cells
SpeciesHuman
Pub Med ID18585103
FileView PDF

Blocking

Blocking DetailsBlocking Buffer: Protein Block Solution (DakoCytomation, Glostrup, Denmark), Blocking Time: 1 hour, Blocking Temp: RT

Primary Anitbody

Dilution RatioDilution Ratio:1:100, Incubation Time/Temp:1hr @ RT, Incubation Dilution Buffer:Ab Diluent w/ Background Reducing Components

Details

Detection NotesVisualization Method: LSAB+System-HRP and DAB+Chromogen, Negative Control: Rabbit polyclonal IgG
Fixation DetailsSample Preparation: OCT Embedded Cryosection, cell culture

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for OGG1 Antibody (NB100-106). (Showing 1 - 2 of 2 FAQs).

  1. Would you please help confirm if NB100-106 would work with human OGG1 alpha?
    • NB100-106 has been validated for use in human, primate and rat for the following applications: Western Blot, IHC-Paraffin and IHC-Frozen. The immunogen used for this antibody maps to the N-terminal region between amino acids 1-100. I believe OGG1 isoforms differ in the C-terminal regional and all have a conserved N-terminus which would indicate to me that our antibody would recognize all isoforms. To be sure, take a close look at the isoform of interest in and compare its sequence to OGG1. If the differences lie outside the 1-100 amino acid range then we are confident the antibody will work for OGG1 alpha.
  2. Is there a difference between Lot#J1 and Lot#L1 for Immunohistochemistry ? What does this product change for product specification between Lot#J1 and Lot#L1?
    • No, there should not have any significant differences between these two lots, J-1 and L-1 applying to any application. The different is the production time for the lots, as they could be bled, purified, and/or gone through other manufacturing processes at the different time points. However sometimes some minor issues do happen, and we call it "lot-to-lot" variations. These minor changes could readily due to how close the breeding time respective to the boosting injections (the closer, the higher titers of IgG), the healthy conditions of the hosts (infection or others), etc. But the specificities nor applicability of the different lots should not be changed.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-106AF350
Alexa Fluor 405 NB100-106AF405
Alexa Fluor 488 NB100-106AF488
Alexa Fluor 532 NB100-106AF532
Alexa Fluor 594 NB100-106AF594
Alexa Fluor 647 NB100-106AF647
Alexa Fluor 700 NB100-106AF700
Alexa Fluor 750 NB100-106AF750
Allophycocyanin/Cy7 NB100-106APCCY7
Biotin NB100-106B
DyLight 350 NB100-106UV
DyLight 405 NB100-106V
DyLight 488 NB100-106G
DyLight 550 NB100-106R
DyLight 594 NB100-106DL594
DyLight 650 NB100-106C
DyLight 680 NB100-106FR
DyLight 755 NB100-106IR
FITC NB100-106F
HRP NB100-106H
Janelia Fluor 549 NB100-106JF549
Janelia Fluor 646 NB100-106JF646
PE/Atto594 NB100-106PEATT594
PE/Cy5.5 NB100-106PECY55
PE/Cy7 NB100-106PECY7

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Blogs on OGG1.

You down with Ogg? Yeah, you know me
8-hydroxyguanine is a form of oxidative DNA damage where free radicals cause G:C to T:A transversions within the backbone. In E. coli, three DNA repair enzymes exist to prevent the mutagenic effects of 8-hydroxyguanine mutations. One of these enzymes,...  Read full blog post.

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Recent Reviews

4.5
5
2
4
2
3
0
2
0
1
0

TIRZAH LAJUS
11/25/2014
Application: IP
Species: Human

Anonymous
06/30/2014
Application: Flow
Species: Human

Saniya Fayzullina
11/12/2012
Application: WB
Species: Human

Bioinformatics

Gene Symbol OGG1
Entrez
Uniprot