OGG1 Antibody - BSA Free

Images

 
Immunohistochemistry-Paraffin: OGG1 Antibody [NB100-106] - Analysis of OGG1 in human skin tissues. Image courtesy of product review by Arash Javeri.
Western Blot: OGG1 Antibody [NB100-106] - - Analysis of Ogg1 expression in Jurkat whole cell lysate.
Immunohistochemistry: OGG1 Antibody [NB100-106] - Figure 5 IHC-P analysis of hOGG1 protein expression in serous ovarian cancer: (A) Normal ovarian tissue treated with anti-hOGG1 antibody, biotinylated anti-rabbit IgG ...read more
Immunocytochemistry/ Immunofluorescence: OGG1 Antibody [NB100-106] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated ...read more
Immunohistochemistry-Frozen: OGG1 Antibody [NB100-106] - Detection of OGG1 in formaldehyde-fixed frozen sections of the substantia nigra from a Rhesus macaque (Macaca mulatta) using NB100-106 at 10 ug/mL. Photo courtesy ...read more
Flow (Intracellular): OGG1 Antibody [NB100-106] - An intracellular stain was performed on HeLa cells with NB100-106C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized ...read more
Immunohistochemistry: OGG1 Antibody [NB100-106] - The H&E staining ((a)-(c)) and IHC staining for oxidative DNA damage, 8-oxo-dGuo ((d)-(g)), and DNA repair enzyme hOGG1 ((h)-(j)) in control subjects and tumor and ...read more
Western Blot: OGG1 Antibody [NB100-106] - The protein expression levels of HVL and LVL sorted cells by western blotting. The ATM, phospho-ATM, GPX2, MRE11, OGG1, and XPC levels were measured by western blot analysis. ...read more
Immunohistochemistry-Paraffin: OGG1 Antibody [NB100-106] - Comparison of the expression of OGG1 protein in normal colon tissue, polyp and cancer tissue of adenoma (AD, n = 68) and carcinoma (CRC, n = 103) patients. ...read more
Flow Cytometry: OGG1 Antibody [NB100-106] - An intracellular stain was performed on HeLa cells with OGG1 Antibody NB100-106 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then ...read more
Immunohistochemistry: OGG1 Antibody [NB100-106] - Staining of human tonsil, germinal center and mantle zone.
Flow Cytometry: OGG1 Antibody [NB100-106] - Baseline Ogg1 expression in human PBMC from a healthy donor. Image from verified.customer review.
Flow Cytometry: OGG1 Antibody [NB100-106] - An intracellular stain was performed on Jurkat Cells with NB100-106 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Pm, RbSpecies Glossary
Applications WB, ELISA, Flow, Flow-IC, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

OGG1 Antibody - BSA Free Summary

Immunogen
A peptide derived from human Ogg1 (within amino acids 1-100). [UniProt# O15527]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
OGG1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:100 - 1:200
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:25-1:100
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500-1:1000
Application Notes
Use in Immunoprecipitation Co-IP (PMID: 20956573) and ELISA (PMID: 19506022) has been reported in the literature. Use in Immunoblotting reported in multiple pieces of scientific literature. In WB, it recognizes a band at ~39 kDa, representing Ogg1. Flow data from customer review. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Jurkat Whole Cell Lysate
Control Peptide
OGG1 Peptide (NB100-106PEP)
Reviewed Applications
Read 5 Reviews rated 3.8
using
NB100-106 in the following applications:

Publications
Read Publications using
NB100-106 in the following applications:

  • 1 publication
  • IB
    5 publications
  • 13 publications
  • IHC
    12 publications
  • 2 publications
  • 2 publications
  • IP
    3 publications
  • WB
    50 publications

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 16651612). Use in Porcine reported in scientific literature (PMID:28846530).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for OGG1 Antibody - BSA Free

  • 8-hydroxyguanine DNA glycosylase
  • 8-oxoguanine DNA glycosylase
  • AP lyase
  • DNA-apurinic or apyrimidinic site lyase
  • HOGG1
  • MMH
  • MUTMOGH1HMMH
  • N-glycosylase/DNA lyase
  • OGG1

Background

8-hydroxyguanine, a form of oxidative DNA damage induced by free radicals, causes G:C to T:A transversion. In E. coli, three DNA repair enzymes exist to prevent the mutagenic effects of 8-hydroxyguanine. One of these enzymes, MutM, was found to have a functional yeast (yOgg1) and human (hOgg1) homologue. hOgg1 proteins efficiently release the 8-hydroxyguanine opposite the pyrimidine from DNA and cleave the AP site in a manner similar to bacterial and yeast enzymes. Genetic backgrounds in control of the repair of damaged DNA are involved in the susceptibility to cancer development. The hOgg1 gene has been mapped to region 3p26.2, a region showing loss of heterozygosity (LOH) in a variety of cancers. In particular, 3p25-p26 is a common LOH region in lung cancer. Recent work has demonstrated that Ogg plays an important role in CAG expansion, a characteristic of several neurodegenerative diseases. Ogg appears to be responsible for progressive expansion of poly-Q tracts in response to oxidative damage. Thus, Ogg provides a direct link between DNA damage and toxicity in neurons.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-116
Species: Hu, Mu, Pm, Rt
Applications: ChIP, ChIP, ELISA, GS, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, PLA, Simple Western, WB
NBP1-87154
Species: Hu
Applications: ICC/IF, IHC, IHC-P, KD, WB
NBP2-16182
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
AF3296
Species: Hu, Mu, Rt
Applications: WB
NB100-108
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
H00004595-M01
Species: Hu, Mu
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, KD, WB
NB200-103
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NBP2-47358
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB100-109
Species: Hu, Mu, Rt
Applications: IB, ICC/IF, IHC, IHC-Fr, IHC-P, KD, KO, Simple Western, Single-Cell Western, WB
NBP2-38600
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NB600-326
Species: ET
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, Simple Western, WB
NBP3-03839
Species: Hu, Mu, Rt
Applications: IHC, WB
NB100-165
Species: Dr, Ha, Hu
Applications: ICC/IF (-), IP, WB
NB500-704
Species: ChHa, Hu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
AF3398
Species: Hu, Mu, Rt
Applications: ICC, KO, Simple Western, WB
NBP2-27335
Species: Hu, Mu, Rt(-)
Applications: CyTOF-ready, Flow, ICC/IF, WB
NBP2-24915
Species: Bv, Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NBP2-67301
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB

Publications for OGG1 Antibody (NB100-106)(116)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Porcine, Primate, Rabbit.

We have publications tested in 8 applications: ELISA, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB.


Filter By Application
ELISA
(1)
IB
(5)
ICC/IF
(13)
IHC
(12)
IHC-Fr
(2)
IHC-P
(2)
IP
(3)
WB
(50)
All Applications
Filter By Species
Human
(44)
Mouse
(18)
Rat
(17)
Porcine
(1)
Primate
(1)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 116. Show All 116 Publications.
Publications using NB100-106 Applications Species
Jung JY, Nam JY, Ryu KS et al. Salivary Immunoglobulin Gamma-3 Chain C Is a Promising Noninvasive Biomarker for Systemic Lupus Erythematosus International journal of molecular sciences Jan 29 2021 [PMID: 33573068] (WB, Human) WB Human
Kulikova E, Boreyko A, Bulanova T et al. Visualization of complex DNA damage along accelerated ions tracks EPJ Web Conf. 2018 Apr 18 (ICC/IF, Human) ICC/IF Human
van der Slikke EC, Star BS, van Meurs M, et al. Sepsis is associated with mitochondrial DNA damage and a reduced mitochondrial mass in the kidney of patients with sepsis-AKI Critical care (London, England) Jan 25 2021 [PMID: 33494815] (ICC/IF, Human) ICC/IF Human
Vitry G, Paulin R, Grobs Y et al. Oxidized DNA Precursors Cleanup by NUDT1 Contributes to Vascular Remodeling in PAH Am J Respir Crit Care Med Oct 6 2020 [PMID: 33021405]
Yang L, Liu G, Li X et al. Small GTPase RAB6 deficiency promotes alveolar progenitor cell renewal and attenuates PM2.5-induced lung injury and fibrosis Cell Death Dis Oct 4 2020 [PMID: 33012781] (WB, Mouse) WB Mouse
Yang L, Liu G, Fu L et al. DNA repair enzyme OGG1 promotes alveolar progenitor cell renewal and relieves PM2.5-induced lung injury and fibrosis Ecotoxicol. Environ. Saf. Sep 22 2020 [PMID: 32977282] (WB, Human) WB Human
Pan G, Deshpande M, Pang H et al. 4-Hydroxy-2-nonenal attenuates 8-oxoguanine DNA glycosylase 1 activity J. Cell. Biochem. Jul 6 2020 [PMID: 32628320]
Xie X, Chen Y, Liu J et al. High glucose induced endothelial cell reactive oxygen species via OGG1/PKC/NADPH oxidase pathway Life Sciences Jun 1 2020 [PMID: 32497631] (WB, ICC/IF, Human, Mouse) WB, ICC/IF Human, Mouse
Kim Sj, Cheresh P, Jablonski Rp et Al. Mitochondrial 8-Oxoguanine DNA Glycosylase Mitigates Alveolar Epithelial Cell PINK1 Deficiency, Mitochondrial DNA Damage, Apoptosis and Lung Fibrosis Am. J. Physiol. Lung Cell Mol. Physiol. Mar 25 2020 [PMID: 32209025] (WB, Mouse) WB Mouse
Sabnam S, Rizwan H, Pal S, Pal A CEES-induced ROS accumulation regulates mitochondrial complications and inflammatory response in keratinocytes Chem. Biol. Interact. Apr 25 2020 [PMID: 32142722] (IB, Mouse) IB Mouse
Show All 116 Publications.

Reviews for OGG1 Antibody (NB100-106) (5) 3.85

Average Rating: 3.8
(Based on 5 reviews)
We have 5 reviews tested in 2 species: Human, Mouse.

Reviews using NB100-106:
Filter by Applications
Simple Western
(1)
IP
(1)
Flow
(1)
WB
(1)
IHC
(1)
All Applications
Filter by Species
Human
(4)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Simple Western OGG1 NB100-106
Enlarge
1
reviewed by:
Verified Customer
Simple Western Mouse 05/19/2020
View

Summary

ApplicationSimple Western
Sample Tested Mouse brain,Mouse Heart Tissue, Sample Amount: 100 ug,mouse liver
SpeciesMouse
LotR-2

Comments

Comments*Low Rating Note: Novus Innovators Program - new species or application used on a primary antibody.
Immunoprecipitation OGG1 NB100-106
Enlarge
4
reviewed by:
TIRZAH LAJUS
IP Human 11/25/2014
View

Summary

ApplicationImmunoprecipitation
Sample TestedMRC5 WCE- IP with Ogg1 ab and immunoblot with Ogg1 ab
SpeciesHuman

Blocking

Blocking Details5% milk in TBS tween 0,1%, 1h @ RT

Secondary Antibody

Secondary Descriptiongoat-anti-mouse HRP

Details

Detection NotesDetection using ECL from Amersham and Kodak films
Immunoprecipitation DetailsIP Gbeads, incubation with ab o/n @4C, 3x wash with elution buffer
Flow Cytometry OGG1 NB100-106
Enlarge
5
reviewed by:
Verified Customer
Flow Human 06/30/2014
View

Summary

ApplicationFlow Cytometry
Sample TestedHuman PBMC
SpeciesHuman

Primary Anitbody

Dilution Ratio1:100 in BD Perm/Wash Buffer
Western Blot OGG1 NB100-106
Enlarge
4
reviewed by:
Saniya Fayzullina
WB Human 11/12/2012
View

Summary

ApplicationWestern Blot
Sample Testedhuman recombinant Ogg1, mouse skeletal muscle whole lysate, mouse spinal cord whole lysate
SpeciesHuman
CommentsThis antibody worked on western blot on human recombinant protein, but not on mouse whole protein lysates from skeletal muscle and spinal cord. It was clean using milk blocking buffer. It was very dirty using BSA blocking buffer.

Blocking

Blocking Details2.5% Milk, 0.1% Tween80 blocking buffer 2x30minutes at room temperature

Primary Anitbody

Dilution Ratio1:2000 in milk blocking buffer, 4C overnight

Secondary Antibody

Secondary Descriptiongoat anti-rabbit IgG, HRP (Invitrogen)
Secondary Concentration1:10000 in milk blocking buffer

Details

Detection NotesECL, film exposed for 1 hour

Comments

CommentsThis antibody worked on western blot on human recombinant protein, but not on mouse whole protein lysates from skeletal muscle and spinal cord. It was clean using milk blocking buffer. It was very dirty using BSA blocking buffer.
Immunohistochemistry OGG1 NB100-106
Enlarge
5
reviewed by:
Verified Customer
IHC Human 05/17/2011
View

Summary

ApplicationImmunohistochemistry
Sample TestedHuman foreskin, adult ski, engineered human skin, keratinocytes, HaCaT cells
SpeciesHuman
Pub Med ID18585103
FileView PDF

Blocking

Blocking DetailsBlocking Buffer: Protein Block Solution (DakoCytomation, Glostrup, Denmark), Blocking Time: 1 hour, Blocking Temp: RT

Primary Anitbody

Dilution RatioDilution Ratio:1:100, Incubation Time/Temp:1hr @ RT, Incubation Dilution Buffer:Ab Diluent w/ Background Reducing Components

Details

Detection NotesVisualization Method: LSAB+System-HRP and DAB+Chromogen, Negative Control: Rabbit polyclonal IgG
Fixation DetailsSample Preparation: OCT Embedded Cryosection, cell culture

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for OGG1 Antibody (NB100-106). (Showing 1 - 2 of 2 FAQs).

  1. Would you please help confirm if NB100-106 would work with human OGG1 alpha?
    • NB100-106 has been validated for use in human, primate and rat for the following applications: Western Blot, IHC-Paraffin and IHC-Frozen. The immunogen used for this antibody maps to the N-terminal region between amino acids 1-100. I believe OGG1 isoforms differ in the C-terminal regional and all have a conserved N-terminus which would indicate to me that our antibody would recognize all isoforms. To be sure, take a close look at the isoform of interest in and compare its sequence to OGG1. If the differences lie outside the 1-100 amino acid range then we are confident the antibody will work for OGG1 alpha.
  2. Is there a difference between Lot#J1 and Lot#L1 for Immunohistochemistry ? What does this product change for product specification between Lot#J1 and Lot#L1?
    • No, there should not have any significant differences between these two lots, J-1 and L-1 applying to any application. The different is the production time for the lots, as they could be bled, purified, and/or gone through other manufacturing processes at the different time points. However sometimes some minor issues do happen, and we call it "lot-to-lot" variations. These minor changes could readily due to how close the breeding time respective to the boosting injections (the closer, the higher titers of IgG), the healthy conditions of the hosts (infection or others), etc. But the specificities nor applicability of the different lots should not be changed.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional OGG1 Products

Bioinformatics Tool for OGG1 Antibody (NB100-106)

Discover related pathways, diseases and genes to OGG1 Antibody (NB100-106). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for OGG1 Antibody (NB100-106)

Discover more about diseases related to OGG1 Antibody (NB100-106).
 

Pathways for OGG1 Antibody (NB100-106)

View related products by pathway.

PTMs for OGG1 Antibody (NB100-106)

Learn more about PTMs related to OGG1 Antibody (NB100-106).
 

Research Areas for OGG1 Antibody (NB100-106)

Find related products by research area.

Blogs on OGG1.

You down with Ogg? Yeah, you know me
8-hydroxyguanine is a form of oxidative DNA damage where free radicals cause G:C to T:A transversions within the backbone. In E. coli, three DNA repair enzymes exist to prevent the mutagenic effects of 8-hydroxyguanine mutations. One of these enzymes,...  Read full blog post.

Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

3.8
5
2
4
2
3
0
2
0
1
1

Verified Customer
05/19/2020
Application: Simple Western
Species: Mouse

TIRZAH LAJUS
11/25/2014
Application: IP
Species: Human

Verified Customer
06/30/2014
Application: Flow
Species: Human

Bioinformatics

Gene Symbol OGG1
Entrez
Uniprot