OGG1 Antibody

Images

 
Western Blot: OGG1 Antibody [NB100-106] - Analysis of Ogg1 recombinant protein using Ogg1 antibody at 1 ug/ml.
Immunocytochemistry/ Immunofluorescence: OGG1 Antibody [NB100-106] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were ...read more
Immunohistochemistry-Paraffin: OGG1 Antibody [NB100-106] - Analysis of OGG1 in human skin tissues. Image courtesy of product review by Arash Javeri.
Flow Cytometry: OGG1 Antibody [NB100-106] - Baseline Ogg1 expression in human PBMC from a healthy donor. Image from confirmed customer review.
Western Blot: OGG1 Antibody [NB100-106] - - Analysis of Ogg1 expression in Jurkat whole cell lysate.
Western Blot: OGG1 Antibody [NB100-106] - 1, 5, 10 ng titration of human recombinant Ogg1 protein, detected by NB 100-106.
Immunohistochemistry-Frozen: OGG1 Antibody [NB100-106] - Detection of OGG1 in formaldehyde fixed frozen sections of the substantia nigra from a Rhesus macaque (Macaca mulatta) using NB100-106 at 10 ug/ml. Photo ...read more
Immunohistochemistry: OGG1 Antibody [NB100-106] - Staining of tonsil, germinal center and mantle zone.

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

OGG1 Antibody Summary

Immunogen
A peptide derived from human Ogg1 (within amino acids 1-100). [UniProt# O15527]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
OGG1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:100 - 1:500
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:25-1:100
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:10-1:500
Application Notes
This Ogg1 antibody is useful for Immunohistochemistry on frozen and paraffin-embedded sections, Immunoprecipitation Co-IP (PMID: 20956573), ELISA (PMID: 19506022) and Western Blot. In WB, it recognizes a band at ~39 kDa, representing Ogg1. In ICC/IF nuclear staining was observed in Hek293 cells. Flow data from customer review. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Jurkat Lysate (NB800-PC2)
Control Peptide
OGG1 Peptide (NB100-106PEP)
Reviewed Applications
Read 4 Reviews rated 4.5
using
NB100-106 in the following applications:

Publications
Read Publications using
NB100-106 in the following applications:

  • 1 publication
  • IB
    4 publications
  • 8 publications
  • IHC
    9 publications
  • 2 publications
  • 2 publications
  • IP
    3 publications
  • WB
    36 publications

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for OGG1 Antibody

  • 8-hydroxyguanine DNA glycosylase
  • 8-oxoguanine DNA glycosylase
  • AP lyase
  • DNA-apurinic or apyrimidinic site lyase
  • HOGG1
  • MMH
  • MUTMOGH1HMMH
  • N-glycosylase/DNA lyase
  • OGG1

Background

8-hydroxyguanine, a form of oxidative DNA damage induced by free radicals, causes G:C to T:A transversion. In E. coli, three DNA repair enzymes exist to prevent the mutagenic effects of 8-hydroxyguanine. One of these enzymes, MutM, was found to have a functional yeast (yOgg1) and human (hOgg1) homologue. hOgg1 proteins efficiently release the 8-hydroxyguanine opposite the pyrimidine from DNA and cleave the AP site in a manner similar to bacterial and yeast enzymes. Genetic backgrounds in control of the repair of damaged DNA are involved in the susceptibility to cancer development. The hOgg1 gene has been mapped to region 3p26.2, a region showing loss of heterozygosity (LOH) in a variety of cancers. In particular, 3p25-p26 is a common LOH region in lung cancer. Recent work has demonstrated that Ogg plays an important role in CAG expansion, a characteristic of several neurodegenerative diseases. Ogg appears to be responsible for progressive expansion of poly-Q tracts in response to oxidative damage. Thus, Ogg provides a direct link between DNA damage and toxicity in neurons.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for OGG1 Antibody (NB100-106)(87)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Primate, Rabbit.

We have publications tested in 8 applications: ELISA, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB.


Filter By Application
ELISA
(1)
IB
(4)
ICC/IF
(8)
IHC
(9)
IHC-Fr
(2)
IHC-P
(2)
IP
(3)
WB
(36)
All Applications
Filter By Species
Human
(32)
Mouse
(12)
Rat
(14)
Primate
(1)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 87. Show All 87 Publications.
Publications using NB100-106 Applications Species
Rivera-Barahona A, Alonso-Barroso E, Perez B et al. Treatment with antioxidants ameliorates oxidative damage in a mouse model of propionic acidemia. Mol. Genet. Metab. 2017 Jul 25 [PMID: 28774709] (Mouse) Mouse
Pillai VB, Kanwal A, Fang YH et al. Honokiol, an activator of Sirtuin-3 (SIRT3) preserves mitochondria and protects the heart from doxorubicin-induced cardiomyopathy in mice. Oncotarget. 2017 May 23 [PMID: 28423723] (IP, Mouse) IP Mouse
Wang SC, Lai KR, Li CY et al. The Paradoxical Effects of Different Hepatitis C Viral Loads on Host DNA Damage and Repair Abilities PLoS ONE Jan 4 2017 [PMID: 28052067] (WB, Human) WB Human
Yang Jl, Chen Wy, Chen Yp et al. Activation of GLP-1 Receptor Enhances Neuronal Base Excision Repair via PI3K-AKT-Induced Expression of Apurinic/Apyrimidinic Endonuclease 1. Theranostics Oct 4 2016 [PMID: 27698937] (WB) WB
Nikitaki Z, Nikolov V, Mavragani IV et al. Measurement of complex DNA damage induction and repair in human cellular systems after exposure to ionizing radiations of varying linear energy transfer (LET). Free Radic Res 2016 Nov [PMID: 27593437] (ICC/IF, Human) ICC/IF Human
Tarry-Adkins Jl, Fernandez-Twinn Ds, Chen Jh et al. Poor maternal nutrition and accelerated postnatal growth induces an accelerated aging phenotype and oxidative stress in skeletal muscle of male rats. Dis Model Mech Sep 1 2016 [PMID: 27585884]
Leon J, Sakumi K, Castillo E et al. Corrigendum: 8-Oxoguanine accumulation in mitochondrial DNA causes mitochondrial dysfunction and impairs neuritogenesis in cultured adult mouse cortical neurons under oxidative conditions. Sci Rep Apr 30 2016 [PMID: 27128497]
Chaisiriwong L, Wanitphakdeedecha R, Sitthinamsuwan P et al. A Case-Control Study of Involvement of Oxidative DNA Damage and Alteration of Antioxidant Defense System in Patients with Basal Cell Carcinoma: Modulation by Tumor Removal. Oxid Med Cell Longev 2016 Jan 13 [PMID: 27057281] (IHC) IHC
Moreli JB, Santos JH, Lorenzon-Ojea AR et al. Hyperglycemia Differentially Affects Maternal and Fetal DNA Integrity and DNA Damage Response Int. J. Biol. Sci. Mar 29 2016 [PMID: 27019630]
Kohno Y, Yamamoto H, Hirahashi M et al. Reduced MUTYH, MTH1, and OGG1 expression and TP53 mutation in diffuse-type adenocarcinoma of gastric cardia. Hum Pathol 2016 Jun [PMID: 26980051]
Show All 87 Publications.

Reviews for OGG1 Antibody (NB100-106) (4) 4.54

Average Rating: 4.5
(Based on 4 reviews)
We have 4 reviews tested in 1 species: Human.
We have 4 reviews tested in 4 application: IP, Flow, WB, IHC.

Reviews using NB100-106:
Filter by Applications
IP
(1)
Flow
(1)
WB
(1)
IHC
(1)
All Applications
Filter by Species
Human
(4)
All Species
Images Ratings Applications Species Date Details
Immunoprecipitation OGG1 NB100-106
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reviewed by:
TIRZAH LAJUS
IP Human 11/25/2014
View

Summary

ApplicationImmunoprecipitation
Sample TestedMRC5 WCE- IP with Ogg1 ab and immunoblot with Ogg1 ab
SpeciesHuman

Blocking

Blocking Details5% milk in TBS tween 0,1%, 1h @ RT

Secondary Antibody

Secondary Descriptiongoat-anti-mouse HRP

Details

Detection NotesDetection using ECL from Amersham and Kodak films
Immunoprecipitation DetailsIP Gbeads, incubation with ab o/n @4C, 3x wash with elution buffer
Flow Cytometry OGG1 NB100-106
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reviewed by:
Anonymous
Flow Human 06/30/2014
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Summary

ApplicationFlow Cytometry
Sample TestedHuman PBMC
SpeciesHuman

Primary Anitbody

Dilution Ratio1:100 in BD Perm/Wash Buffer
Western Blot OGG1 NB100-106
Enlarge
reviewed by:
Saniya Fayzullina
WB Human 11/12/2012
View

Summary

ApplicationWestern Blot
Sample Testedhuman recombinant Ogg1, mouse skeletal muscle whole lysate, mouse spinal cord whole lysate
SpeciesHuman
CommentsThis antibody worked on western blot on human recombinant protein, but not on mouse whole protein lysates from skeletal muscle and spinal cord. It was clean using milk blocking buffer. It was very dirty using BSA blocking buffer.

Blocking

Blocking Details2.5% Milk, 0.1% Tween80 blocking buffer 2x30minutes at room temperature

Primary Anitbody

Dilution Ratio1:2000 in milk blocking buffer, 4C overnight

Secondary Antibody

Secondary Descriptiongoat anti-rabbit IgG, HRP (Invitrogen)
Secondary Concentration1:10000 in milk blocking buffer

Details

Detection NotesECL, film exposed for 1 hour

Comments

CommentsThis antibody worked on western blot on human recombinant protein, but not on mouse whole protein lysates from skeletal muscle and spinal cord. It was clean using milk blocking buffer. It was very dirty using BSA blocking buffer.
Immunohistochemistry OGG1 NB100-106
Enlarge
reviewed by:
Anonymous
IHC Human 05/17/2011
View

Summary

ApplicationImmunohistochemistry
Sample TestedHuman foreskin, adult ski, engineered human skin, keratinocytes, HaCaT cells
SpeciesHuman
Pub Med ID18585103
FileView PDF

Blocking

Blocking DetailsBlocking Buffer: Protein Block Solution (DakoCytomation, Glostrup, Denmark), Blocking Time: 1 hour, Blocking Temp: RT

Primary Anitbody

Dilution RatioDilution Ratio:1:100, Incubation Time/Temp:1hr @ RT, Incubation Dilution Buffer:Ab Diluent w/ Background Reducing Components

Details

Detection NotesVisualization Method: LSAB+System-HRP and DAB+Chromogen, Negative Control: Rabbit polyclonal IgG
Fixation DetailsSample Preparation: OCT Embedded Cryosection, cell culture

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for OGG1 Antibody (NB100-106). (Showing 1 - 2 of 2 FAQs).

  1. Would you please help confirm if NB100-106 would work with human OGG1 alpha?
    • NB100-106 has been validated for use in human, primate and rat for the following applications: Western Blot, IHC-Paraffin and IHC-Frozen. The immunogen used for this antibody maps to the N-terminal region between amino acids 1-100. I believe OGG1 isoforms differ in the C-terminal regional and all have a conserved N-terminus which would indicate to me that our antibody would recognize all isoforms. To be sure, take a close look at the isoform of interest in and compare its sequence to OGG1. If the differences lie outside the 1-100 amino acid range then we are confident the antibody will work for OGG1 alpha.
  2. Is there a difference between Lot#J1 and Lot#L1 for Immunohistochemistry ? What does this product change for product specification between Lot#J1 and Lot#L1?
    • No, there should not have any significant differences between these two lots, J-1 and L-1 applying to any application. The different is the production time for the lots, as they could be bled, purified, and/or gone through other manufacturing processes at the different time points. However sometimes some minor issues do happen, and we call it "lot-to-lot" variations. These minor changes could readily due to how close the breeding time respective to the boosting injections (the closer, the higher titers of IgG), the healthy conditions of the hosts (infection or others), etc. But the specificities nor applicability of the different lots should not be changed.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-106AF405
Alexa Fluor 488 Labeled NB100-106AF488
Alexa Fluor 647 Labeled NB100-106AF647
Alexa Fluor 700 Labeled NB100-106AF700
Biotin Labeled NB100-106B
DyLight 405 Labeled NB100-106V
DyLight 488 Labeled NB100-106G
DyLight 550 Labeled NB100-106R
DyLight 650 Labeled NB100-106C
FITC Labeled NB100-106F
HRP Labeled NB100-106H

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Blogs on OGG1.

You down with Ogg? Yeah, you know me
8-hydroxyguanine is a form of oxidative DNA damage where free radicals cause G:C to T:A transversions within the backbone. In E. coli, three DNA repair enzymes exist to prevent the mutagenic effects of 8-hydroxyguanine mutations. One of these enzymes,...  Read full blog post.

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Recent Reviews

4.5
5
2
4
2
3
0
2
0
1
0

TIRZAH LAJUS
11/25/2014
Application: IP
Species: Human

Anonymous
06/30/2014
Application: Flow
Species: Human

Saniya Fayzullina
11/12/2012
Application: WB
Species: Human

Bioinformatics

Gene Symbol OGG1
Entrez
Uniprot