DNA Ligase IV Antibody

Images

 
Western Blot: DNA Ligase IV Antibody [NBP2-16182] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase IV antibody diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: DNA Ligase IV Antibody [NBP2-16182] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: DNA Ligase IV Antibody [NBP2-16182] - Immunohistochemical analysis of paraffin-embedded OVCA, using antibody at 1:250 dilution.
Western Blot: DNA Ligase IV Antibody [NBP2-16182] - Sample (30 ug of whole cell lysate) A: U87-MG 7. 5% SDS PAGE gel, diluted at 1:1000.
Western Blot: DNA Ligase IV Antibody [NBP2-16182] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase IV antibody diluted at 1:500.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

DNA Ligase IV Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human DNA ligase IV. The exact sequence is proprietary.
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LIG4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
104 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Sheep (83%), Bovine (86%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DNA Ligase IV Antibody

  • DNA joinase
  • DNA ligase 4
  • DNA ligase IV
  • DNA repair enzyme
  • EC 6.5.1.1
  • ligase IV, DNA, ATP-dependent
  • Polydeoxyribonucleotide synthase [ATP] 4
  • polynucleotide ligase
  • sealase

Background

The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol LIG4
Uniprot