Amphiregulin Antibody [Biotin]

• Reactivity: Hu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Amphiregulin Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant human Amphiregulin
  Val107-Lys184
  Accession # P15514
• Specificity: Detects human Amphiregulin in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross‑reactivity with rmAR, rhEGF and rhTGF-alpha is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: AREG
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Human Amphiregulin Antibody (Catalog # MAB262)
  ELISA Detection: Human Amphiregulin Biotinylated Antibody (Catalog # BAF262)
  Standard: Recombinant Human Amphiregulin (Catalog # 262-AR)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Amphiregulin Antibody [Biotin]

• Amphiregulin
• AR
• AREG
• AREGB
• Colorectum cell-derived growth factor
• CRDGF
• MGC13647
• schwannoma-derived growth factor
• SDGF

Background

Amphiregulin (AR) is a member of the EGF family of cytokines which is comprised of at least ten proteins including EGF, TGF-alpha , HB-EGF, and the various heregulins. All of these cytokines are synthesized as transmembrane precursors and are characterized by the presence of one or several EGF structural units in their extracellular domain. The soluble forms of these cytokines are released by proteolytic cleavage. Amphiregulin was originally isolated from the conditioned media of a PMA-treated MCF-7 human breast carcinoma cell line. The AR cDNA encodes a 252 amino acid (aa) residue transmembrane precursor. Multiple forms of native AR containing either 78 or 84 aa residues and both N- and O-linked oligosaccharides have been found. Amphiregulin mRNA expression can be detected in numerous carcinoma cell lines and the epithelial cells of various human tissues including colon, stomach, breast, ovary, kidney, etc.

Human AR stimulates the proliferation of various human and mouse keratinocytes, mammary epithelial cells and some fibroblasts. AR is also a growth inhibitor for various tumor cell lines. In certain colon carcinoma cell lines, AR has been shown to be an autocrine growth factor. Amphiregulin can bind to the EGF receptor. It has been suggested that in certain cell types, AR bioactivity may be mediated through the EGF receptor. The 98 aa residue long form of recombinant amphiregulin has shown to be approximately 5 - 10 fold more active than the 78 aa residue form of recombinant AR in an in vitro proliferation assay using Balb/3T3 fibroblasts.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Amphiregulin Antibody (BAF262)(8)

Publications using BAF262

• AM Tsou, H Yano, CN Parkhurst, T Mahlakõiv, C Chu, W Zhang, Z He, KJ Jarick, C Zhong, GG Putzel, M Hatazaki, JRI IBD Li, IC Lorenz, D Andrew, P Balderes, CSN Klose, SA Lira, D Artis Neuropeptide regulation of non-redundant ILC2 responses at barrier surfaces Nature, 2022-11-02;0(0):. 2022-11-02 [PMID: 36323781] (Flow Cytometry, Human)
• Karen Agaronyan, Lokesh Sharma, Bharat Vaidyanathan, Keith Glenn, Shuang Yu, Charles Annicelli et al. Tissue remodeling by an opportunistic pathogen triggers allergic inflammation Immunity 2022-05-01 [PMID: 35483356]
• LMD Canto, SS Cury, MC Barros-Fil, BEC Kupper, MDFS Begnami, C Scapulatem, RF Carvalho, FA Marchi, DA Olsen, JS Madsen, BM Havelund, S Aguiar, SR Rogatto Locally advanced rectal cancer transcriptomic-based secretome analysis reveals novel biomarkers useful to identify patients according to neoadjuvant chemoradiotherapy response Sci Rep, 2019-06-18;9(1):8702. 2019-06-18 [PMID: 31213644] (ELISA Detection, Human)
• ADAM17 substrate release in proximal tubule drives kidney fibrosis JCI Insight, 2016-08-18;1(13):. 2016-08-18 [PMID: 27642633] (IHC, Mouse)
• Stoll S, Stuart P, Lambert S, Gandarillas A, Rittie L, Johnston A, Elder J Membrane-Tethered Intracellular Domain of Amphiregulin Promotes Keratinocyte Proliferation. J Invest Dermatol, 2016-02-01;136(2):444-52. 2016-02-01 [PMID: 26802239] (ELISA Development, Human)
• Maria U. Latasa, Fabiana Salis, Raquel Urtasun, Oihane Garcia-Irigoyen, Maria Elizalde, Iker Uriarte et al. Regulation of Amphiregulin Gene Expression by beta -Catenin Signaling in Human Hepatocellular Carcinoma Cells: A Novel Crosstalk between FGF19 and the EGFR System PLoS ONE 2012-12-20 [PMID: 23285165]
• Hassan Lemjabbar-Alaoui, Sukhvinder S. Sidhu, Aklilu Mengistab, Marianne Gallup, Carol Basbaum TACE/ADAM-17 Phosphorylation by PKC-Epsilon Mediates Premalignant Changes in Tobacco Smoke-Exposed Lung Cells PLoS ONE 2011-03-15 [PMID: 21423656]
• Gonzalez RM, Seurynck-Servoss SL, Crowley SA Development and validation of sandwich ELISA microarrays with minimal assay interference. J. Proteome Res., 2008-04-19;7(6):2406-14. 2008-04-19 [PMID: 18422355] (ELISA Microarray Development, Human)

Bioinformatics

• Gene Symbol: AREG
• Uniprot:
  • P15514()