alcohol dehydrogenase 1B Antibody (OTI4F12)

Images

 
Western Blot: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - Analysis of extracts (10ug) from 9 Human tissue by using anti-ADH1B monoclonal antibody at 1:1000 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: ...read more
Immunocytochemistry/ Immunofluorescence: alcohol dehydrogenase 1B Antibody (4F12) [NBP2-00649] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY alcohol dehydrogenase 1B.
Immunohistochemistry-Paraffin: alcohol dehydrogenase 1B Antibody (4F12) [NBP2-00649] - Staining of paraffin-embedded Human lymph node tissue using anti-alcohol dehydrogenase 1B mouse monoclonal antibody.
Flow Cytometry: alcohol dehydrogenase 1B Antibody (4F12) [NBP2-00649] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-alcohol ...read more
Western Blot: alcohol dehydrogenase 1B Antibody (4F12) [NBP2-00649] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY alcohol dehydrogenase 1B (Right lane) cDNA for 48 hrs and ...read more
Western Blot: alcohol dehydrogenase 1B Antibody (4F12) [NBP2-00649] Analysis of extracts (35ug) from 9 different cell lines by using anti-alcohol dehydrogenase 1B monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: alcohol dehydrogenase 1B Antibody (4F12) [NBP2-00649] - Immunofluorescent staining of HeLa cells using anti-alcohol dehydrogenase 1B mouse monoclonal antibody.
Immunohistochemistry-Paraffin: alcohol dehydrogenase 1B Antibody (4F12) [NBP2-00649] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-alcohol dehydrogenase 1B mouse monoclonal antibody.
Immunohistochemistry-Paraffin: alcohol dehydrogenase 1B Antibody (4F12) [NBP2-00649] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-alcohol dehydrogenase 1B mouse monoclonal antibody.
Immunohistochemistry-Paraffin: alcohol dehydrogenase 1B Antibody (4F12) [NBP2-00649] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-alcohol dehydrogenase 1B mouse monoclonal antibody.
Immunohistochemistry-Paraffin: alcohol dehydrogenase 1B Antibody (4F12) [NBP2-00649] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-alcohol dehydrogenase 1B mouse monoclonal antibody.
Immunohistochemistry-Paraffin: alcohol dehydrogenase 1B Antibody (4F12) [NBP2-00649] - Staining of paraffin-embedded Human liver tissue using anti-alcohol dehydrogenase 1B mouse monoclonal antibody.
Immunohistochemistry-Paraffin: alcohol dehydrogenase 1B Antibody (4F12) [NBP2-00649] - Staining of paraffin-embedded Human lung tissue using anti-alcohol dehydrogenase 1B mouse monoclonal antibody.
Flow Cytometry: alcohol dehydrogenase 1B Antibody (4F12) [NBP2-00649] - Analysis of Hela cells, using anti-alcohol dehydrogenase 1B antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: alcohol dehydrogenase 1B Antibody (4F12) [NBP2-00649] - Analysis of Jurkat cells, using anti-alcohol dehydrogenase 1B antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Rt, CaSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
OTI4F12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

alcohol dehydrogenase 1B Antibody (OTI4F12) Summary

Immunogen
Full length human recombinant protein of human ADH1B (NP_000659) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ADH1B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150
Theoretical MW
39.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for alcohol dehydrogenase 1B Antibody (OTI4F12)

  • ADH, beta subunit
  • ADH2alcohol dehydrogenase 1B
  • alcohol dehydrogenase 1B (class I), beta polypeptide
  • alcohol dehydrogenase 2 (class I), beta polypeptide
  • Alcohol dehydrogenase subunit beta
  • aldehyde reductase
  • DKFZp686C06125
  • EC 1.1.1
  • EC 1.1.1.1

Background

The protein encoded by this gene is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This encoded protein, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for alcohol dehydrogenase 1B Antibody (NBP2-00649) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-70092AF350
Alexa Fluor 405 NBP2-70092AF405
Alexa Fluor 488 NBP2-70092AF488
Alexa Fluor 532 NBP2-70092AF532
Alexa Fluor 594 NBP2-70092AF594
Alexa Fluor 647 NBP2-70092AF647
Alexa Fluor 700 NBP2-70092AF700
Alexa Fluor 750 NBP2-70092AF750
Allophycocyanin NBP2-70092APC
Allophycocyanin/Cy7 NBP2-70092APCCY7
Biotin NBP2-70092B
DyLight 350 NBP2-70092UV
DyLight 405 NBP2-70092V
DyLight 488 NBP2-70092G
DyLight 550 NBP2-70092R
DyLight 594 NBP2-70092DL594
DyLight 650 NBP2-70092C
DyLight 680 NBP2-70092FR
DyLight 755 NBP2-70092IR
FITC NBP2-70092F
HRP NBP2-70092H
Janelia Fluor 549 NBP2-70092JF549
Janelia Fluor 646 NBP2-70092JF646
PE NBP2-70092PE
PE/Atto594 NBP2-70092PEATT594
PE/Cy5.5 NBP2-70092PECY55
PE/Cy7 NBP2-70092PECY7
PerCP NBP2-70092PCP

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Bioinformatics

Gene Symbol ADH1B
Entrez