Amphiregulin Antibody

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Amphiregulin was detected in immersion fixed MCF‑7 human breast cancer cell line using Goat Anti-Human Amphiregulin Antigen Affinity-purified Poly­clonal Antibody (Catalog # AF262) at 10 µg/mL for 3 hours at room ...read more
Amphiregulin was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human Amphiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF262) at 15 µg/mL overnight at 4 °C. ...read more
Western blot shows conditioned media from T47D human breast cancer cell line untreated (-) or treated (+) with PMA and PHA for 3 days. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Amphiregulin Antigen ...read more
Recombinant Human Amphiregulin (Catalog # 262‑AR) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, B/N, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Amphiregulin Antibody Summary

Immunogen
E. coli-derived recombinant human Amphiregulin
Val107-Lys184
Accession # P15514
Specificity
Detects human Amphiregulin in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse (rm) Amphiregulin, rmEpiregulin, recombinant human (rh) HB-EGF, and rhTGF-alpha is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
AREG
Purity
Immunogen affinity purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunocytochemistry 5-15 ug/mL
  • Neutralization 0.3-1 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF262 in the following applications:

Publications
Read Publications using
AF262 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Amphiregulin Antibody

  • Amphiregulin
  • AR
  • AREG
  • AREGB
  • Colorectum cell-derived growth factor
  • CRDGF
  • MGC13647
  • schwannoma-derived growth factor
  • SDGF

Background

Amphiregulin (AR) is a member of the EGF family of cytokines which is comprised of at least ten proteins including EGF, TGF-alpha, HB-EGF, and the various heregulins. All of these cytokines are synthesized as transmembrane precursors and are characterized by the presence of one or several EGF structural units in their extracellular domain. The soluble forms of these cytokines are released by proteolytic cleavage. Amphiregulin was originally isolated from the conditioned media of a PMA-treated MCF-7 human breast carcinoma cell line. The AR cDNA encodes a 252 amino acid (aa) residue transmembrane precursor. Multiple forms of native AR containing either 78 or 84 aa residues and both N- and O-linked oligosaccharides have been found. Amphiregulin mRNA expression can be detected in numerous carcinoma cell lines and the epithelial cells of various human tissues including colon, stomach, breast, ovary, kidney, etc.

Human AR stimulates the proliferation of various human and mouse keratinocytes, mammary epithelial cells and some fibroblasts. AR is also a growth inhibitor for various tumor cell lines. In certain colon carcinoma cell lines, AR has been shown to be an autocrine growth factor. Amphiregulin can bind to the EGF receptor. It has been suggested that in certain cell types, AR bioactivity may be mediated through the EGF receptor. The 98 aa residue long form of recombinant amphiregulin has shown to be approximately 5 - 10 fold more active than the 78 aa residue form of recombinant AR in an in vitro proliferation assay using Balb/3T3 fibroblasts.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Amphiregulin Antibody (AF262)(13)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: Functional Assay, ICC, IHC Not Specified, IHC Paraffin-embedded, Neut, WB.


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Functional Assay
(1)
ICC
(3)
IHC Not Specified
(2)
IHC Paraffin-embedded
(1)
Neut
(5)
WB
(1)
All Applications
Filter By Species
Human
(12)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using AF262 Applications Species
W Dong, L Liu, Y Dou, M Xu, T Liu, S Wang, Y Zhang, B Deng, B Wang, H Cao Deoxycholic acid activates epidermal growth factor receptor and promotes intestinal carcinogenesis by ADAM17-dependent ligand release J. Cell. Mol. Med., 2018;0(0):. 2018 [PMID: 29956475] (Neut,, Mouse) Neut Mouse
Noguchi S, Saito A, Horie M, Mikami Y, Suzuki H, Morishita Y, Ohshima M, Abiko Y, Mattsson J, Konig H, Lohr M, Edlund K, Botling J, Micke P, Nagase T An integrative analysis of the tumorigenic role of TAZ in human non-small cell lung cancer. Clin Cancer Res, 2014;20(17):4660-72. 2014 [PMID: 24951773] (IHC Not Specified,, Human) IHC Not Specified Human
Chen, Z, Chen, J, Gu, Y, Hu, C, Li, J-L, Lin, S, Shen, H, Cao, C, Gao, R, Li, J, Ha, P K, Kaye, F J, Griffin, J D, Wu, L Aberrantly activated AREG-EGFR signaling is required for the growth and survival of CRTC1-MAML2 fusion-positive mucoepidermoid carcinoma cells. Oncogene, 2014;33(29):3869-77. 2014 [PMID: 23975434] (IHC Not Specified,, Human) IHC Not Specified Human
Nakayama H, Fukuda S, Matsushita N, Nishida-Fukuda H, Inoue H, Shirakata Y, Hashimoto K, Higashiyama S Human antigen R-mediated mRNA stabilization is required for ultraviolet B-induced autoinduction of amphiregulin in keratinocytes. J Biol Chem, 2013;288(15):10338-48. 2013 [PMID: 23430747] (WB,, Human) WB Human
Tinhofer I, Klinghammer K, Weichert W, Knodler M, Stenzinger A, Gauler T, Budach V, Keilholz U Expression of amphiregulin and EGFRvIII affect outcome of patients with squamous cell carcinoma of the head and neck receiving cetuximab-docetaxel treatment. Clin. Cancer Res., 2011;17(15):5197-204. 2011 [PMID: 21653686] (IHC Paraffin-embedded,, Human) IHC Paraffin-embedded Human
Egloff AM, Rothstein ME, Seethala R, Siegfried JM, Grandis JR, Stabile LP Cross-talk between estrogen receptor and epidermal growth factor receptor in head and neck squamous cell carcinoma. Clin. Cancer Res., 2009;15(21):6529-40. 2009 [PMID: 19825947] (Functional Assay,, Human) Functional Assay Human
Isokane M, Hieda M, Hirakawa S, Shudou M, Nakashiro K, Hashimoto K, Hamakawa H, Higashiyama S Plasma-membrane-anchored growth factor pro-amphiregulin binds A-type lamin and regulates global transcription. J. Cell. Sci., 2008;121(0):3608-18. 2008 [PMID: 18946024] (ICC,, Human) ICC Human
Eckstein N, Servan K, Girard L, Cai D, von Jonquieres G, Jaehde U, Kassack MU, Gazdar AF, Minna JD, Royer HD Epidermal growth factor receptor pathway analysis identifies amphiregulin as a key factor for cisplatin resistance of human breast cancer cells. J. Biol. Chem., 2007;283(2):739-50. 2007 [PMID: 17942395] (Neut,, Human) Neut Human
Rumelhard M, Ramgolam K, Hamel R, Marano F, Baeza-Squiban A Expression and role of EGFR ligands induced in airway cells by PM2.5 and its components. Eur. Respir. J., 2007;30(6):1064-73. 2007 [PMID: 17804444] (Neut,, Human) Neut Human
Liu M, Yang SC, Sharma S, Luo J, Cui X, Peebles KA, Huang M, Sato M, Ramirez RD, Shay JW, Minna JD, Dubinett SM EGFR signaling is required for TGF-beta 1 mediated COX-2 induction in human bronchial epithelial cells. Am. J. Respir. Cell Mol. Biol., 2007;37(5):578-88. 2007 [PMID: 17600311] (Neut,, Human) Neut Human
Show All 13 Publications.

Review for Amphiregulin Antibody (AF262) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF262:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 01/07/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 22445895
SpeciesHuman

Blocking

Blocking DetailsSee PMID 22445895

Details

Detection NotesSee PMID 22445895

Comments

CommentsPublished: See PMID 22445895Independent review from antibodyresource.com

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Recent Reviews

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Anonymous
01/07/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol AREG
Entrez
Uniprot