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HAF109). A specific band was detected for Amphiregulin at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox" target="_blank">
262-AR) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Amphiregulin (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Amphiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF262). The ND50 is typically 0.3-1 µg/mL." class="big_lightbox" target="_blank">
989-AR), Recombinant Human Amphiregulin (Catalog # 262-AR), and Recombinant Mouse HB-EGF. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse Amphiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF989) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Amphiregulin at approximately 16-25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_lightbox" target="_blank">
989-AR) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Amphiregulin (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Amphiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF989). The ND50 is typically 0.3-1.5 µg/mL." class="big_lightbox" target="_blank">
262-AR) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Amphiregulin (50 ng/mL) is neutralized (green line) by increasing concentrations of Human Amphiregulin Monoclonal Antibody (Catalog # MAB262). The ND50 is typically 5-15 µg/mL." class="big_lightbox" target="_blank">
The protein encoded by the Amphiregulin gene is a member of the epidermal growth factor family. It is an autocrine growth factor aswell as a mitogen for astrocytes, Schwann cells, and fibroblasts. It is related to epidermal growth factor (EGF) andtransforming growth factor alpha (TGF-alpha). This protein interacts with the EGF/TGF-alpha receptor to promote thegrowth of normal epithelial cells and inhibits the growth of certain aggressive carcinoma cell lines. This encodedprotein is associated with a psoriasis-like skin phenotype. (provided by RefSeq)
![Western Blot Amphiregulin Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Amphiregulin_AF989_Western_Blot_21594.jpg)
![N/A Amphiregulin [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_Amphiregulin_DY262_ELISA_1858.jpg)
![N/A Amphiregulin [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_Amphiregulin_DAR00_ELISA_578.jpg)
![N/A Amphiregulin [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_Amphiregulin_DY989_ELISA_2295.jpg)
![Immunohistochemistry Amphiregulin Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Amphiregulin_AF989_Immunohistochemistry_18726.jpg)
![Neutralization Amphiregulin Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Amphiregulin_AF989_Block_Neutralize_8992.jpg)
![Immunocytochemistry/ Immunofluorescence Amphiregulin Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af989_mouse-amphiregulin-affinity-purified-polyclonal-ab-immunocytochemistry-immunofluorescence-12122025963922.jpg)
![Flow Cytometry Amphiregulin Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af989_mouse-amphiregulin-affinity-purified-polyclonal-ab-flow-cytometry-121220259224811.jpg)
![Immunocytochemistry/ Immunofluorescence Amphiregulin Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af989_mouse-amphiregulin-affinity-purified-polyclonal-ab-immunocytochemistry-immunofluorescence-12122025961611.jpg)
![Flow Cytometry Amphiregulin Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af989_mouse-amphiregulin-affinity-purified-polyclonal-ab-flow-cytometry-12122025921116.jpg)
![Western Blot Amphiregulin Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af989_mouse-amphiregulin-affinity-purified-polyclonal-ab-western-blot-1212202592597.jpg)
![Western Blot Amphiregulin Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af989_mouse-amphiregulin-affinity-purified-polyclonal-ab-western-blot-12122025930522.jpg)
![Western Blot Amphiregulin Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af989_mouse-amphiregulin-affinity-purified-polyclonal-ab-western-blot-121220259311211.jpg)
![Immunocytochemistry/ Immunofluorescence Amphiregulin Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af989_mouse-amphiregulin-affinity-purified-polyclonal-ab-immunocytochemistry-immunofluorescence-121220259305220.jpg)
![Western Blot Amphiregulin Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af989_mouse-amphiregulin-affinity-purified-polyclonal-ab-western-blot-12122025926916.jpg)
![Western Blot Amphiregulin Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af989_mouse-amphiregulin-affinity-purified-polyclonal-ab-western-blot-12122025930527.jpg)
![Western Blot Amphiregulin Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af989_mouse-amphiregulin-affinity-purified-polyclonal-ab-western-blot-121220259323724.jpg)
![Western Blot Amphiregulin Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af989_mouse-amphiregulin-affinity-purified-polyclonal-ab-western-blot-12122025932158.jpg)
![Western Blot Amphiregulin Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af989_mouse-amphiregulin-affinity-purified-polyclonal-ab-western-blot-12122025924520.jpg)
![N/A Amphiregulin [HRP]](http://images.novusbio.com/fullsize/elisa/Amphiregulin_DAR00_ELISA_459.jpg)
