Amphiregulin Antibodies: Novus Biologicals

AF262

Amphiregulin Antibody

(1 Review)

100 μg

AF262

HAF109). A specific band was detected for Amphiregulin at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

<b>Biological Strategies Validation. </b>Western blot shows conditioned media from T47D human breast cancer cell line untreated (-) or treated (+) with PMA and PHA for 3 days. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Amphiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF262) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF109). A specific band was detected for Amphiregulin at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows conditioned media from T47D human breast cancer cell line untreated (-) or treated (+) with PMA and PHA for 3 days. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Amphiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF262) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Amphiregulin at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL001) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

<b>Biological Strategies Validation. </b>Amphiregulin was detected in immersion fixed MCF-7 human breast cancer cell line using Goat Anti-Human Amphiregulin Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF262) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # <a class=

NL001) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

Amphiregulin was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human Amphiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF262) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

262-AR) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Amphiregulin (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Amphiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF262). The ND₅₀ is typically 0.3-1 µg/mL." class="big_lightbox">

Recombinant Human Amphiregulin (Catalog # <a class=

262-AR) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Amphiregulin (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Amphiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF262). The ND₅₀ is typically 0.3-1 µg/mL." class="big_thumb" />

N. gonorrhoeae infection causes an increase of membrane bound amphiregulin that co-localizes with the site of bacterial adherence.Non-permeabilized Me-180 cells were infected with N. gonorrhoeae for 6 hours. Amphiregulin was detected by using polyclonal antibodies against amphiregulin (MBS220633), followed by secondary IgG antibodies conjugated with fluorofor Alexa 488 nm. (A) Representative immunofluorescence images visualizing the protein levels of amphiregulin on the plasma membrane in control cells and infected cells. Cellular DNA and bacterial DNA was stained with the cell-permeable dye Hoechst 33342. (B) High magnification images of infected Me-180 cells. Image showing the co-localization between surface bound amphiregulin (red) and bacterial DNA staining (blue). (C) Flow cytometry histogram showing the protein levels of plasma membrane bound amphiregulin in non-permeabilized cells. Amphiregulin staining on uninfected control cells is shown in grey while staining of infected cells is shown with a black line. The histogram is representative of two independent experiments. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/21298020), licensed under a CC-BY license. Not internally tested by R&D Systems.

262-AR) was serially diluted and captured by Mouse Anti-Human Amphiregulin Monoclonal Antibody (Catalog # MAB262) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Amphiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF262) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

262-AR) was serially diluted and captured by Mouse Anti-Human Amphiregulin Monoclonal Antibody (Catalog # MAB262) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Amphiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF262) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

262-AR) was serially diluted and captured by Mouse Anti-Human Amphiregulin Monoclonal Antibody (Catalog # MAB262) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Amphiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF262) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

262-AR) was serially diluted and captured by Mouse Anti-Human Amphiregulin Monoclonal Antibody (Catalog # MAB262) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Amphiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF262) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF262U-100UG
Alexa Fluor 405	AF262V-100UG
Alexa Fluor 488	AF262G-100UG
Alexa Fluor 532	AF262X-100UG
Alexa Fluor 594	AF262T-100UG
Alexa Fluor 647	AF262R-100UG
Alexa Fluor 700	AF262N-100UG
Alexa Fluor 750	AF262S-100UG
Alexa Fluor Plus 405	AF262AFP405-100UG
Alexa Fluor Plus 488	AF262AFP488-100UG
Alexa Fluor Plus 555	AF262AFP555-100UG
Alexa Fluor Plus 594	AF262AFP594-100UG
Alexa Fluor Plus 647	AF262AFP647-100UG
Alexa Fluor Plus 680	AF262AFP680-100UG
Allophycocyanin	AF262A
Biotin	BAF262
CoraFluor 1	AF262CL1
DyLight 350	AF262D
DyLight 405	AF262E
DyLight 488	AF262K
DyLight 550	AF262L
DyLight 594	AF262M
DyLight 650	AF262W
DyLight 680	AF262Y
DyLight 755	AF262Z
FITC	AF262F
HRP	AF262H
Janelia Fluor 525	AF262JF525
Janelia Fluor 549	AF262I
Janelia Fluor 646	AF262J
Janelia Fluor 669	AF262JF669
PE	AF262P
PerCP	AF262C
mFluor Violet 450 SE	AF262MFV450
mFluor Violet 500 SE	AF262MFV500
mFluor Violet 610 SE	AF262MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF262-SP

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AF989

Amphiregulin Antibody [Unconjugated]

(25 Publications)

100 μg

AF989

989-AR), Recombinant Human Amphiregulin (Catalog # 262-AR), and Recombinant Mouse HB-EGF. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse Amphiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF989) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Amphiregulin at approximately 16-25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_lightbox">

Western blot shows 25 ng of Recombinant Mouse Amphiregulin (Catalog # <a class=

989-AR), Recombinant Human Amphiregulin (Catalog # 262-AR), and Recombinant Mouse HB-EGF. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse Amphiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF989) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Amphiregulin at approximately 16-25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." alt="Western blot shows 25 ng of Recombinant Mouse Amphiregulin (Catalog # 989-AR), Recombinant Human Amphiregulin (Catalog # 262-AR), and Recombinant Mouse HB-EGF. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse Amphiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF989) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Amphiregulin at approximately 16-25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to keratinocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

Amphiregulin was detected in immersion fixed frozen sections of mouse skin using Goat Anti-Mouse Amphiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF989) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to keratinocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

989-AR) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Amphiregulin (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Amphiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF989). The ND₅₀ is typically 0.3-1.5 µg/mL." class="big_lightbox">

Recombinant Mouse Amphiregulin (Catalog # <a class=

989-AR) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Amphiregulin (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Amphiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF989). The ND₅₀ is typically 0.3-1.5 µg/mL." class="big_thumb" />

GPR174 regulates neovascularization by inhibiting AREG expression in Tregs. d Representative immunofluorescent images of AREG (red) & DAPI (blue) staining in Tregs isolated from ischemic muscle of WT & Gpr174−/Y mice 7 days after HLI. Scale bar, 10 μm. Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36473866), licensed under a CC-BY license. Not internally tested by R&D Systems.

GPR174 regulates neovascularization by inhibiting AREG expression in Tregs. b Serum AREG, IL-10, & VEGF protein content in Rag1−/− mice receiving Tregs 7 days after adoptive transplantation experiments (n = 6 for PBS → Rag1−/− mice; n = 7 for wild-type Tregs→Rag1−/− mice; n = 7 for GPR174-deficient Tregs→Rag1−/− mice). Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36473866), licensed under a CC-BY license. Not internally tested by R&D Systems.

MyD88 silencing and NF kappa B inhibition restrain extracellular AREG-induced macrophage pyroptosis. NLRP3, CASPASE1-p20, and GSDMD-N expression levels were detected in extracellular AREG-induced Myd88−/− and Trif−/−BMDM via Western blot (A, B). NLRP3, CASPASE1-p20, and GSDMD-N expression levels were detected in the inhibitor of NF kappa B (P65) (20 μM) pretreating extracellular AREG-induced BMDM for 2 h via Western blot (C, D). Data are presented as mean ± SEM (n ≥ 3). *P < 0.05, **P < 0.01, ***P < 0.001 vs. Control. ns, no significient; AREG, amphiregulin; BMDM, bone marrow-derived macrophages; EGFR, epidermal growth factor receptor; GSDMD, gasdermin D. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/40292295), licensed under a CC-BY license. Not internally tested by R&D Systems.

EGFR inhibition and TLR4 silencing impair AREG-induced macrophage pyroptosis. BMDM was stimulated with AREG+ATP or LPS+ATP, and the expression of NLRP3, p-P65, p-I kappa B, CASPASE-1-p20, and GSDMD-N was detected via Western blot (A-C). BMDM was stimulated with AREG+ATP or LPS+ATP, and oligomerization of ASC was detected using immunofluorescence (D, E). Experimental diagram of AREG-induced macrophage pyroptosis. For the priming step, BMDM was treated with AREG for 2.5 as the first signal and the ATP as the second signal (F). NLRP3, CASPASE-1-p20, and GSDMD-N expressions were detected in the EGFR inhibitor (1 mM) pretreating AREG +ATP-induced BMDM for 4 h through Western blot (G, J). NLRP3, CASPASE-1-p20, and GSDMD-N expressions were detected in AREG +ATP-induced TLR4−/−BMDM via Western blot (H, K). The expression of IL-1b and IL-18 was detected in the supernatant of AREG +ATP-induced TLR4−/−BMDM via ELISA (I). Data are presented as mean ± SEM (n ≥ 3). *P < 0.05, **P < 0.01, ***P < 0.001 vs. Control. ns, no significant; AREG, amphiregulin; BMDM, bone marrow-derived macrophages; EGFR, epidermal growth factor receptor; GSDMD, gasdermin D; ATP, adenosine triphosphate. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/40292295), licensed under a CC-BY license. Not internally tested by R&D Systems.

Dynamic expression of AREG in sepsis. RAW264.7 cells were stimulated with LPS (100 ng/mL or 1 μg/mL) for 1, 3, 6, 12, and 24 h. AREG mRNA and protein expressions were detected using RT-PCR and ELISA (A-C). WT C57BL/6 mice were intraperitoneally injected with LPS (20 mg/kg) and constructed with the CLP model, whereas AREG protein expression in serum was detected via ELISA (D). BMDM was stimulated with LPS (100 ng/mL or 1 μg/mL) for 12 h, and AREG protein expression was detected via Immunofluorescence (E). Data presented as mean ± SEM (n ≥ 3). *P < 0.05, **P < 0.01, ***P < 0.001 vs. Control. AREG, amphiregulin; WT, wild type; BMDM, bone marrow-derived macrophages; CLP, cecal ligation and puncture. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/40292295), licensed under a CC-BY license. Not internally tested by R&D Systems.

DTT-pretreated extracellular AREG restrains macrophage pyroptosis. NLRP3, CASPASE1-p20, and GSDMD-N expressions were detected in LPS-induced BMDM after DTT (1 mM) or H2O2 (100 μM) pretreating LPS for 1 h (A, B). NLRP3, Caspase1-p20, and GSDMD-N expression were detected in AREG-induced BMDM after DTT (1 mM) or H2O2 (100 μM) pretreating AREG for 1 h (C, D). Data are presented as mean ± SEM (n ≥ 3).*P < 0.05, **P < 0.01, ***P < 0.001 vs. Control. ns, no significient; AREG, amphiregulin; BMDM, bone marrow-derived macrophages; EGFR, epidermal growth factor receptor; GSDMD, gasdermin D. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/40292295), licensed under a CC-BY license. Not internally tested by R&D Systems.

Neutralizing extracellular AREG decreases LPS-induced TLR4 expression and pyroptosis in macrophages. LPS-induced BMDM was pretreated with a neutralizing antibody of AREG. p-EGFR, TLR4, and GSDMD-N expression levels were detected via Western blot and immunofluorescence (A-C, E, F), TLR4 expression and ASC oligomerization was detected through immunofluorescence (D, G, H). Formation of pyrosomes (red arrows) was detected using electron microscopy, scale bars, 2 μm (I). Data are presented as mean ± SEM (n ≥ 3).*P < 0.05, **P < 0.01, ***P < 0.001 vs. Control. AREG, amphiregulin. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/40292295), licensed under a CC-BY license. Not internally tested by R&D Systems.

989-AR) was serially diluted and captured by Goat Anti-Mouse Amphiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF989) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Amphiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF989) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

989-AR) was serially diluted and captured by Goat Anti-Mouse Amphiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF989) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Amphiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF989) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

989-AR) was serially diluted and captured by Goat Anti-Mouse Amphiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF989) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Amphiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF989) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

989-AR) was serially diluted and captured by Goat Anti-Mouse Amphiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF989) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Amphiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF989) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF989U-100UG
Alexa Fluor 405	AF989V-100UG
Alexa Fluor 488	AF989G-100UG
Alexa Fluor 532	AF989X-100UG
Alexa Fluor 594	AF989T-100UG
Alexa Fluor 647	AF989R-100UG
Alexa Fluor 700	AF989N-100UG
Alexa Fluor 750	AF989S-100UG
Alexa Fluor Plus 405	AF989AFP405-100UG
Alexa Fluor Plus 488	AF989AFP488-100UG
Alexa Fluor Plus 555	AF989AFP555-100UG
Alexa Fluor Plus 594	AF989AFP594-100UG
Alexa Fluor Plus 647	AF989AFP647-100UG
Alexa Fluor Plus 680	AF989AFP680-100UG
Allophycocyanin	AF989A
Biotin	BAF989
CoraFluor 1	AF989CL1
DyLight 350	AF989D
DyLight 405	AF989E
DyLight 488	AF989K
DyLight 550	AF989L
DyLight 594	AF989M
DyLight 650	AF989W
DyLight 680	AF989Y
DyLight 755	AF989Z
FITC	AF989F
HRP	AF989H
Janelia Fluor 525	AF989JF525
Janelia Fluor 549	AF989I
Janelia Fluor 646	AF989J
Janelia Fluor 669	AF989JF669
PE	AF989P
PerCP	AF989C
mFluor Violet 450 SE	AF989MFV450
mFluor Violet 500 SE	AF989MFV500
mFluor Violet 610 SE	AF989MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF989-SP

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MAB262

Amphiregulin Antibody (31221)

(20 Publications)

100 μg

MAB262

262-AR) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Amphiregulin (50 ng/mL) is neutralized (green line) by increasing concentrations of Human Amphiregulin Monoclonal Antibody (Catalog # MAB262). The ND₅₀ is typically 5-15 µg/mL." class="big_lightbox">

262-AR) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Amphiregulin (50 ng/mL) is neutralized (green line) by increasing concentrations of Human Amphiregulin Monoclonal Antibody (Catalog # MAB262). The ND₅₀ is typically 5-15 µg/mL." alt="Recombinant Human Amphiregulin (Catalog # 262-AR) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Amphiregulin (50 ng/mL) is neutralized (green line) by increasing concentrations of Human Amphiregulin Monoclonal Antibody (Catalog # MAB262). The ND₅₀ is typically 5-15 µg/mL." class="big_thumb" />

262-AR) was serially diluted and captured by Mouse Anti-Human Amphiregulin Monoclonal Antibody (Catalog # MAB262) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Amphiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF262) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

262-AR) was serially diluted and captured by Mouse Anti-Human Amphiregulin Monoclonal Antibody (Catalog # MAB262) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Amphiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF262) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB262U-100UG
Alexa Fluor 405	FAB262V-100UG
Alexa Fluor 488	FAB262G-100UG
Alexa Fluor 532	FAB262X-100UG
Alexa Fluor 594	FAB262T-100UG
Alexa Fluor 647	FAB262R-100UG
Alexa Fluor 700	FAB262N-100UG
Alexa Fluor 750	FAB262S-100UG
Alexa Fluor Plus 405	FAB262AFP405-100UG
Alexa Fluor Plus 488	FAB262AFP488-100UG
Alexa Fluor Plus 555	FAB262AFP555-100UG
Alexa Fluor Plus 594	FAB262AFP594-100UG
Alexa Fluor Plus 647	FAB262AFP647-100UG
Alexa Fluor Plus 680	FAB262AFP680-100UG
Allophycocyanin	FAB262A
Biotin	FAB262B
CoraFluor 1	FAB262CL1
DyLight 350	FAB262D
DyLight 405	FAB262E
DyLight 488	FAB262K
DyLight 550	FAB262L
DyLight 594	FAB262M
DyLight 650	FAB262W
DyLight 680	FAB262Y
DyLight 755	FAB262Z
FITC	FAB262F
HRP	FAB262H
Janelia Fluor 525	FAB262JF525
Janelia Fluor 549	FAB262I
Janelia Fluor 646	FAB262J
Janelia Fluor 669	FAB262JF669
PE	FAB262P
PerCP	FAB262C
mFluor Violet 450 SE	FAB262MFV450
mFluor Violet 500 SE	FAB262MFV500
mFluor Violet 610 SE	FAB262MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB262-SP
Carrier Free	MAB262-500

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MAB989

Amphiregulin Antibody (206220) [Unconjugated]

(7 Publications)

100 μg

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB989U-100UG
Alexa Fluor 405	FAB989V-100UG
Alexa Fluor 488	FAB989G-100UG
Alexa Fluor 532	FAB989X-100UG
Alexa Fluor 594	FAB989T-100UG
Alexa Fluor 647	FAB989R-100UG
Alexa Fluor 700	FAB989N-100UG
Alexa Fluor 750	FAB989S-100UG
Alexa Fluor Plus 405	FAB989AFP405-100UG
Alexa Fluor Plus 488	FAB989AFP488-100UG
Alexa Fluor Plus 555	FAB989AFP555-100UG
Alexa Fluor Plus 594	FAB989AFP594-100UG
Alexa Fluor Plus 647	FAB989AFP647-100UG
Alexa Fluor Plus 680	FAB989AFP680-100UG
Allophycocyanin	FAB989A
Biotin	FAB989B
CoraFluor 1	FAB989CL1
DyLight 350	FAB989D
DyLight 405	FAB989E
DyLight 488	FAB989K
DyLight 550	FAB989L
DyLight 594	FAB989M
DyLight 650	FAB989W
DyLight 680	FAB989Y
DyLight 755	FAB989Z
FITC	FAB989F
HRP	FAB989H
Janelia Fluor 525	FAB989JF525
Janelia Fluor 549	FAB989I
Janelia Fluor 646	FAB989J
Janelia Fluor 669	FAB989JF669
PE	FAB989P
PerCP	FAB989C
mFluor Violet 450 SE	FAB989MFV450
mFluor Violet 500 SE	FAB989MFV500
mFluor Violet 610 SE	FAB989MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB989-SP
Carrier Free	MAB989-500

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AB-262-NA

Amphiregulin Antibody

(2 Publications)

1 mg

AB-262-NA

262-AR) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Amphiregulin (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Amphiregulin Polyclonal Antibody (Catalog # AB-262-NA). The ND₅₀ is typically 5-20 µg/mL." class="big_lightbox">

262-AR) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Amphiregulin (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Amphiregulin Polyclonal Antibody (Catalog # AB-262-NA). The ND₅₀ is typically 5-20 µg/mL." alt="Recombinant Human Amphiregulin (Catalog # 262-AR) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Amphiregulin (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Amphiregulin Polyclonal Antibody (Catalog # AB-262-NA). The ND₅₀ is typically 5-20 µg/mL." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Unconjugated

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NBP1-87071

Amphiregulin Antibody - BSA Free

(1 Publication)

0.1 ml

NBP1-87071

Immunohistochemistry-Paraffin: Amphiregulin Antibody [NBP1-87071] - Staining of human Fallopian tube shows moderate membranous/cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: Amphiregulin Antibody [NBP1-87071] - Staining of human liver shows no positivity in hepatocytes, as expected.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP2-15379

Amphiregulin Antibody

(1 Publication)

0.1 ml

NBP2-15379

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP3-41077

Amphiregulin Antibody (A3) - BSA Free

100 ul

NBP3-41077

Immunocytochemistry /Immunofluorescence: Amphiregulin Antibody (A3) [NBP3-41077] - FITC staining on IF; Samples: Human MCF7 cell; Primary Ab: 20ug/ml NBP3-41077; Second Ab: 0.75ug/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody

Reactivity:

Hu, Rt

Species Glossary

Reactivity: Hu, Rt

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP3-41091

Amphiregulin Antibody (A7) - BSA Free

100 ul

NBP3-41091

Immunohistochemistry-Paraffin: Amphiregulin Antibody (A7) [NBP3-41091] - DAB staining on IHC-P; Sample: Human Prostate cancer Tissue; Primary Ab: 20ug/ml NBP3-41091; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP3-41076

Amphiregulin Antibody (A6) - BSA Free

100 ul

NBP3-41076

Immunohistochemistry-Paraffin: Amphiregulin Antibody (A6) [NBP3-41076] - DAB staining on IHC-P; Samples: Human Breast cancer Tissue; Primary Ab: 20ug/ml NBP3-41076; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP3-41096

Amphiregulin Antibody (A2) - BSA Free

100 ul

NBP3-41096

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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AF989AFP405

Amphiregulin Antibody [Alexa Fluor™ Plus 405]

100 μg

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Alexa Fluor Plus 405

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

Amphiregulin Antibodies

Amphiregulin Antibody

Amphiregulin Antibody [Unconjugated]

Amphiregulin Antibody (31221)

Amphiregulin Antibody (206220) [Unconjugated]

Amphiregulin Antibody

Amphiregulin Antibody - BSA Free

Amphiregulin Antibody

Amphiregulin Antibody (A3) - BSA Free

Amphiregulin Antibody (A7) - BSA Free

Amphiregulin Antibody (A6) - BSA Free

Amphiregulin Antibody (A2) - BSA Free

Amphiregulin Antibody [Alexa Fluor™ Plus 405]