AIF-1/Iba1 Antibody

Images

 
Western Blot: AIF-1/Iba1 Antibody [NB100-2833] - (0.5ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NB100-2833] - (2ug/ml) staining of paraffin embedded Mouse Brain. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

AIF-1/Iba1 Antibody Summary

Immunogen
Peptide with sequence C-NKQFLDDPKYSSDED corresponding to internal region according to NP_001614.3.
Marker
pan-Microglia Marker
Specificity
This antibody is expected to recognise isoform 3 (NP_001614.3) only.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
AIF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1.5ug/ml
  • Immunohistochemistry 2 - 4 ug/ml
  • Immunohistochemistry-Paraffin 2-3ug/ml
  • Peptide ELISA detection limit 1:32000
Application Notes
WB: Approx. 17 kDa band observed in human brain (frontal cortex) and peripheral blood Lymphocytes lysates (calculated MW of 16.7 kDa band according to NP_001614.3). IHC-P: Mouse brain shows staining of astrocytes.
Positive Control
Blood Leukocyte Lysate (NB820-59174)
AIF-1/Iba1 Lysate (NBL1-07408)
Publications
Read Publications using
NB100-2833 in the following applications:

  • WB
    1 publication

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for AIF-1/Iba1 Antibody

  • AIF1
  • AIF-1
  • AIF-1IRT1
  • allograft inflammatory factor 1
  • G1
  • IBA1
  • IBA1Em:AF129756.17
  • IbaI
  • interferon gamma responsive transcript
  • Ionized calcium-binding adapter molecule 1
  • IRT1
  • IRT-1
  • Protein G1

Background

This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. The gene expresses three transcripts.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AIF-1/Iba1 Antibody (NB100-2833)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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(1)
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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol AIF1
Entrez
Uniprot