Blood Leukocyte Whole Cell Lysate

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Concentration
5.0 mg/ml

Order Details

Blood Leukocyte Whole Cell Lysate Summary

Specificity
Total Protein - Human Adult Normal Tissue: Peripheral Blood Leukocyte

Applications/Dilutions

Application Notes
Electrophoresis, Enzymatic activity analysis, Protein-protein interaction and Tissue specific expression.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors
Concentration
5.0 mg/ml

Lysate Details for Array

Type
Cell
Tissue
Blood Lymph
Protein State
Native
Subcellular Fraction
Whole

Notes

We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.

Background

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Blood Leukocyte Lysate (NB820-59174). (Showing 1 - 1 of 1 FAQ).

  1. Blood Leukocyte Whole Cell Lysate (NB820-59174): Is this product pooled leukocytes or a single donor, and does it include neutrophils or just PBMCs?
    • The current lot is from a single donor. The lysate is simply from buffy coat. The neutrophils should be included.

Other Available Formats

NB820-59174

Additional Blood Leukocyte Products

Array NB820-59174

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